Antibiotic Resistance in Salmonella Isolated from Tegus (Tupinambis spp.)

In recent years, an increase in human clinical cases of reptile-associated salmonellosis has been identified, and it has been attributed to the increased popularity of these animals as pets. Limited information is available regarding the distribution of Salmonella spp. serotypes in different reptile species and the antimicrobial resistance patterns of Salmonella spp. isolated from pet reptiles. This article describes the prevalence of Salmonella spp., distribution of serotypes, and antibiotic susceptibility patterns from isolates cultured from cloacal swabs obtained from 14 tegu lizards (Tupinambis spp.). Eighteen strains of Salmonella belonging to different serotypes were obtained from the 14 tegu lizards. Of the 18 Salmonella spp. isolates, 8 (44.4%) were from Salmonella subspecies I, with a majority of isolates belonging to the Eastbourne serotype (3 strains), Nottingham serotype (2 strains), and Brancaster serotype (2 strains), and only 1 belonging to the Apapa serotype. Less common serotypes were detected in 5 isolates, including 2 each belonging to Salmonella subspecies II and IIIb, respectively, and 1 to Salmonella subspecies IIIa. The serotype of 5 other Salmonella isolates could not be determined. All 18 isolates were resistant to at least 6 of the antimicrobial drugs tested. These results confirm the potential zoonotic risk from handling reptiles, suggesting that measures to educate the reptile-owning public are necessary.     

Effect of Thermal Processing on Potato Sensory Profile and off-Odours Detection during Storage

One of the most used thermal treatments in potatoes to inactivate enzymes and prevent their oxidation is the boiling process. This treatment significantly decreases the taste of the potato and causes “off-odours” during storage, whatsoever, represents a big problem for the production of potato-based foodstuffs. This study focused on evaluating the sensory profile of potato slices subjected to a boiling process of three Mexican potato varieties (Alpha, Chica and Gallo) and on off-odours detection in potato produced during storage. According to the examined parameters through PanelCheck, the trained panel had a good performance, resulting 8 of the 12 attributes evaluated were significant. The formation of “cardboard-like off-odour” was detected in the Alpha and Chica varieties at the 24 and 33 h of storage, respectively. Chica potato presented this off-odours with at level significantly lower than Alpha potato (about 30% lower). With respect of Gallo variety, this presented different oxidation olfactory perceptions described by the assessors as “burnt note”, detected after 5 h of refrigerated storage. An adequate election of the variety of potato according to processing will allow an effective utilization of this vegetable and the obtention of potato-based foodstuffs much more stable.     

Outcome of tactile conditioning of neonates, or "imprint training" on selected handling measures in foals

Behavioural reactions to selected handling procedures were compared between conditioned, or imprint-trained, and untrained foals raised on the same farm. Nineteen randomly chosen healthy foals were imprint trained at birth and 24 h later (Group A). Twenty-one similar foals that were not imprint-trained served as age-matched controls (Group B). Training began within 10 min of birth and consisted of touch desensitization by gentle rubbing. Each tactile stimulus was repeated 30-50 times over 45-60 min, until the foal no longer resisted the procedure and appeared relaxed. The procedure was then repeated at 24 h of age. At that time a physical examination and blood analysis were performed to assess the foals' health status. Group B animals were not handled except for a brief physical examination and blood analysis at 24 h of age. Thereafter all foals were kept on pastures with their dams with no further handling until they were three months of age. Any foals handled for other reasons before that time were excluded from the study. At three months, each of the 28 foals that completed the study experienced the following handling procedures: acceptance of restraint, haltering, complete physical examination, acceptance of a plastic rebreathing bag, touching the whole body, intramuscular vaccination in the neck, intranasal vaccination, and deworming with oral paste. Response to each procedure was scored (1=not resistant, 2=low resistance, 3=strong resistance, 4=not possible without major physical restraint). Conditioned foals (Group A) were significantly less resistant to touching the front and hind legs and picking up the hind feet (P < 0.05). The administration of vaccines and paste dewormer and the collection of blood were tolerated by the majority of the foals of both groups with no or low resistance. It appeared that neonatal imprint training resulted in a learned behaviour that resulted in decreased self-defence responses towards handling the limbs at three months of age.     

Multi-scale mismatches between urban sprawl and landscape fragmentation create windows of opportunity for conservation development

Context

Urban sprawl and the expanding transportation infrastructure drive land consumption and landscape fragmentation, causing environmental deterioration and loss of species. Current understanding of how these drivers interact to shape landscape fragmentation is still poor. However, a strong correlation between urban sprawl and landscape fragmentation patterns is commonly assumed.

Objectives

Our main objective was to test the strength, non-stationarity, and scale-dependency of the relationship between urban sprawl and landscape fragmentation patterns (‘sprawl-fragmentation relationship’). Subsequently, we propose an extended framework for the links between urban sprawl, expansion of transport infrastructure, and landscape fragmentation.

Methods

We quantified spatial patterns of urban sprawl and landscape fragmentation for mainland Spain at multiple scales. We then fitted global regression models and geographically weighted regression models with metrics of landscape fragmentation and urban sprawl.

Results

Most variation in landscape fragmentation values (almost 80 % on average) is not explained by urban sprawl metrics through global modeling. Local models show substantial improvements in model performance, with an average of 37 % of the variance remaining unexplained. The contribution of urban sprawl to landscape fragmentation patterns varies locally and depends on scale, with higher contributions at coarser scales and at higher organizational levels.

Conclusions

Our investigation revealed three critical characteristics of the sprawl-fragmentation relationship: it does not prevail, is non-stationary, and scale-dependent. We propose four mechanisms that may have resulted in this mismatch: scale, time-lagged development, spatial arrangement of development, and other external variables including teleconnections. These spatial mismatches provide windows of opportunity for conservation through better development strategies.

     

Frequency of appropriate and inappropriate presentation and analysis methods of ordered categorical data in the veterinary dermatology literature from January 2003 to June 2006

Clinical outcomes that are difficult to measure directly are often graded with ordinal scales in the veterinary dermatology literature to approximate objective evaluation. Ordered categorical scales require statistical presentation and analysis methods consistent with the structure of the data. The objective of this study was to determine the frequency of inappropriate presentation and analysis methods of ordered categorical data in the recent veterinary dermatology literature. A total of 62 articles published between 1 January 2003 and 30 June 2006 in 16 journals reported categorical scales and were included in the study. The presentation and analysis methods of ordered categorical data were classified as appropriate or inappropriate based on published recommendations. Forty articles (64.5%) utilized a median of four ordinal scales (range 1-13). Inappropriate presentation methods of ordered categorical data were identified in 23 of 40 articles (57.5%). These included reporting inappropriate summary statistics (n = 17) and summation of ad hoc numerical rating scales (n = 15). Inappropriate analytical methods were used in nine of 40 articles (22.5%). These included inappropriate use of t-tests (n = 3) and analysis of variance (anova, n = 6). The frequency of inappropriate presentation and analysis methods of ordered categorical data in the veterinary dermatology literature is similar to that reported for several fields in the human medical literature. In order to reduce the likelihood of making unwarranted implications or conclusions regarding ordinal data, authors should follow established guidelines for methods of presentation and analysis of ordered categorical scales.