Neurotransmitter release from a vertebrate neuromuscular synapse affected by a food dye

The food dye erythrosine (Erythrosin B; FD & C No. 3) was applied to isolated neuromuscular synapses in the frog, and its effects on the spontaneous quantal release of acetylcholine were examined with electrophysiological techniques. At concentrations of 10 muM or greater this anionic dye produced an irreversible, dose-dependent increase in neurotransmitter release. This increase did not depend on the presence of calcium ions in the bathing medium. These increase did not depend on the presence of calcium ions in the bathing medium. These results suggest that erythrosine might prove a useful pharmacological tool for studying the process of transmitter release, but that its use as a food additive should be reexamined.     

Sequence variants in the RNF212 gene associate with genome-wide recombination rate

The genome-wide recombination rate varies between individuals, but the mechanism controlling this variation in humans has remained elusive. A genome-wide search identified sequence variants in the 4p16.3 region correlated with recombination rate in both males and females. These variants are located in the RNF212 gene, a putative ortholog of the ZHP-3 gene that is essential for recombinations and chiasma formation in Caenorhabditis elegans. It is noteworthy that the haplotype formed by two single-nucleotide polymorphisms (SNPs) associated with the highest recombination rate in males is associated with a low recombination rate in females. Consequently, if the frequency of the haplotype changes, the average recombination rate will increase for one sex and decrease for the other, but the sex-averaged recombination rate of the population can stay relatively constant.     

Development of a dragonfly awareness trail in an African botanical garden

The IUCN/SSC Status Survey and Conservation Action Plan: Dragonflies calls for an increase in educating the public and increasing awareness of dragonflies (Odonata). Dragonflies are conspicuous and attractive, and can ‘stand in’ for other invertebrates in raising awareness of the necessity to conserve invertebrates. While reserves have been set aside for dragonfly awareness and conservation in the northern hemisphere, no such dragonfly reserves appear to exist in the southern hemisphere, despite its rich dragonfly fauna. This paper describes the development of a dragonfly-awareness trail in an established and well-visited botanical garden (The National Botanical Gardens, Pietermaritzburg, South Africa). Correlations between species and environmental variables were significantly high for six measured environmental variables: pH, percentage shade, vegetation (structural and compositional), ambient and water temperature, and water depth. Multivariate analyses of data, classified 20 a priori selected sampling units into four ecologically meaningful biotope types, each with a characteristic dragonfly assemblage. The four biotopes provided potential viewing points. To these were added a further three duplicate biotopes so as to link the trail into a circuit. Questionnaires assessed public awareness of dragonflies, and helped design a preliminary leaflet. A final trail design was drawn up along with an expanded booklet. The trail has now been implemented. Recommendations are made on the minimal scientific underpinning required for future trail design.     

Development of protective immunity against Eimeria tenella and E. acervulina in White Leghorn chickens inoculated repeatedly with high doses of turkey coccidia

Repeated inoculation (immunization) of 2-week-old white leghorn chickens with 10(6) oocysts of the turkey coccidia Eimeria adenoeides or E. meleagrimitis partially protected chickens against moderate challenge with E. tenella or E. acervulina oocysts, but not with E. necatrix oocysts. After challenge, mean weight gains of the immunized chickens and the unchallenged controls did not differ significantly, but weight gains of unimmunized chickens were significantly lower. The mean feed-conversion ratio of the immunized challenged chickens was 3.14, as compared with 4.42 for unimmunized challenged control chickens. In general, immunization did not markedly reduce intestinal lesions. Repeated inoculation of chickens with the turkey coccidium E. gallopavonis failed to produce statistically significant protection against challenge with E. tenella, E. acervulina, or E. necatrix, as determined by weight gain, feed-conversion efficiency, and lesion scores. Antibody profiles of individual chickens did not correlate with protection.     

Preliminary Study in Immature Canine Oocytes Stained with Brilliant Cresyl Blue and Obtained From Bitches with Low and High Progesterone Serum Profiles

This study was conducted: (i) to observe the features and levels of blue colour impregnation in morphologically selected immature canine cumulus oocyte complexes (COCs) stained with the brilliant cresyl blue (BCB) dye, as indicators of quality, and integrity of nuclear oocyte chromatin configuration before in vitro maturation (IVM); (ii) to observe the relationship between the influence of serum progesterone (SP) concentrations from ovary donors and BCB staining of immature dog oocytes. The results showed that out of 138 canine COCs, germinal vesicle (GV) stage prevailed in BCB+ oocytes at percentages of 67.4% (60/89), which were statistically higher than those observed in BCB+/− (52.2%; 23/44) and BCB− (20%; 1/5) oocytes (p = 0.023). Oocytes BCB+ were interpreted as those having completed their growth and therefore possessing the capacity to mature and develop in vitro . Ooplasm and cumulus cells (CCs) of canine oocytes were BCB staining independent. Ooplasm blue colour staining reaction varied between grown oocytes, revealing different levels of glucose-6-phosphate dehydrogenase activity among and within oocytes. Additionally, SP profile of ovary donors was not a relevant indicator for selection of oocytes screened with the BCB stain. Similar numbers of high quality oocytes were observed to be BCB+, BCB+/− and BCB− between groups of females with SP varying from 0 to 2.5 ng/ml (n = 5), and those with SP varying from 2.6 to 16.7 ng/ml (n = 4) (p = 0.680). It may be inferred that bitches with low and high SP profiles have grown oocytes in their ovaries, as determined by the BCB absorbance in their ooplasms.     

USING TISSUE ANALYSIS AS A TOOL TO PREDICT BAHIAGRASS PHOSPHORUS FERTILIZATION REQUIREMENT

Tissue analysis in combination with soil testing has been recently incorporated in the fertilization recommendations for bahiagrass in Florida; however, limited research is available to validate the proposed critical tissue phosphorus (P) level. A greenhouse experiment was conducted to identify the critical minimum tissue P concentration below which bahiagrass dry matter (DM) yield is impacted. Treatments consisted of three nitrogen (N) rates (0, 50, and 100 kg N ha^?1), four P rates (0, 20, 40, 60 kg P₂O₅ ha^?1) and two clipping intervals (28 and 56 d). Bahiagrass yields and tissue P increased in response to P application. Phosphorus uptake showed a quadratic response to P rates when N was applied. Phosphorus recovery increased linearly as P rate increased. Root mass responded to P, even at the smallest (20 kg ha^?1) rate. Data indicated that tissue P concentration of 1.3 (±0.2) g kg^?1 can be used as an indicator of bahiagrass P deficiency.     

A short note on seed dispersal by colobines: the case of the proboscis monkey

Although the role of primates in seed dispersal is generally well recognized, this is not the case for colobines, which are widely distributed in Asian and African tropical forests. Colobines consume leaves, seeds and fruits, usually unripe. A group of proboscis monkeys (Colobinae, Nasalis larvatus) consisting of 1 alpha‐male, 6 adult females and several immatures, was observed from May 2005 to May 2006. A total of 400 fecal samples from focal group members covering 13 months were examined, with over 3500 h of focal observation data on the group members in a forest along the Menanggul River, Sabah, Malaysia. Intact small seeds were only found in 23 of 71 samples in Nov 2005, 15 of 38 in Dec 2005 and 5 of 21 in Mar 2006. Seeds of Ficus (all <1.5 mm in length) were found in all 3 months and seeds from Antidesma thwaitesianum (all <3 mm) and Nauclea subdita (all <2 mm) only in Nov and Dec, which was consistent with members of the study group consuming fruits of these species mostly at these times. To our knowledge, these are the first records of seeds in the fecal samples of colobines. Even if colobines pass relatively few seeds intact, their high abundance and biomass could make them quantitatively significant in seed dispersal. The potential role of colobines as seed dispersers should be considered by colobine researchers.     

Cattle Grazing Distribution in Shortgrass Steppe: Influences of Topography and Saline Soils

The distribution of livestock across heterogeneous landscapes is often uneven, which has important implications for vegetation dynamics and how rangeland managers achieve desired outcomes from these landscapes. Here, we use data from widely available digital elevation models to classify a landscape in the shortgrass steppe with subtle topographic variation using two different approaches: topographic wetness index (TWI) and topographic position classes (TPCs) derived from topographic position indices. We used global positioning system collars to track the grazing locations of cattle within replicate pastures and fit generalized linear mixed models to their locations to quantify the influence of topography on grazing distribution. In addition, we examine the influence of the presence of saline vegetation communities on cattle use of lowlands. The resulting models indicate that TPC more effectively predicts grazing distribution than TWI and that the patterns are strongest in the second half of the growing season (August ? October). Model performance was improved with the inclusion of saline vegetation communities, although the magnitude of cattle grazing time in these communities was not consistent across multiple pastures. These models, in combination with local knowledge, can be used by managers to predict and manage livestock distribution even in landscapes with relatively subtle topographic variability.