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71.
Tong Ly Hiroyuki Fukuoka Asami Otaka Atsushi Hoshino Shigeru Iida Eiji Nitasaka Nobuyoshi Watanabe Tsutomu Kuboyama 《Breeding Science》2012,62(1):99-104
Although Japanese morning glory (Ipomoea nil (L.) Roth.) has been used intensively for genetic studies, DNA markers have not been developed in Ipomoea nil sufficient to cover all chromosomes. Therefore, we conducted microsatellite (simple sequence repeats, SSR) marker development in I. nil for future genetic studies. From 92,662 expressed sequence tag (EST) sequences, 514 unique microsatellite-containing ESTs were identified. Primer pairs were designed automatically in 326 SSRs. Of 150 SSRs examined, 75 showed polymorphisms among strains. A phenogram based on the SSR genotypes revealed the genetic relation among seven Japanese morning glories from five different regions of the world and an ivyleaf morning glory (I. hederacea Jacq.). The developed SSR markers might be applicable for genetic studies of morning glories and their relatives. 相似文献
72.
Nigussie Haregeweyn Bekure Melesse Atsushi Tsunekawa Mitsuru Tsubo Derege Meshesha Bedru Babulo Balana 《Journal of Soils and Sediments》2012,12(2):291-305
Purpose
The Angereb dam in northwestern Ethiopia was commissioned in 1997 to serve as a domestic water supply for 25 years. However, its sustainability is being threatened by rapid sedimentation. The overall objective of this study was to understand reservoir sedimentation in this tropical highland watershed and to propose its mitigating strategies that would contribute to the improved planning and management of reservoirs in similar regions. 相似文献73.
Nakamura A Sasaki F Watanabe K Ojima T Ahn DH Saeki H 《Journal of agricultural and food chemistry》2006,54(25):9529-9534
The effect of the Maillard reaction on the allergenicity of squid tropomyosin (TM) was investigated. When TM was reacted with ribose (TM-ribose), its human-specific IgE-binding ability decreased markedly and alpha-chymotryptic digestibility of TM was also altered at the early stage of the Maillard reaction. On the other hand, the modification of the lysine residues in TM using 2,4,6-trinitrobenzenesulfonic acid had no effect on the allergenicity and alpha-chymotryptic digestibility of TM. Therefore, the structural change in TM induced by the Maillard reaction would cause the reduction of the allergenicity, rather than the block of lysine residues. Although peptic digestion diminished the specific IgE-binding ability of TM, the reduction of the allergenicity by the Maillard reaction remained after peptic digestion. These results suggest that hypersensitive reaction of TM-ribose in the human body might be lower than that of native TM. 相似文献
74.
Kodama S Yamamoto A Sato A Suzuki K Yamashita T Kemmei T Taga A Hayakawa K 《Journal of agricultural and food chemistry》2007,55(16):6547-6552
Chiral resolution of isoxanthohumol (IX) in beer samples was performed by hydroxypropyl-gamma-cyclodextrin-modified micellar electrokinetic chromatography. The optimum running conditions were found to be 20 mM phosphate buffer (pH 7.0) containing 45 mM hydroxypropyl-gamma-cyclodextrin and 100 mM sodium dodecyl sulfate with an effective voltage of +20 kV at 20 degrees C using direct detection at 210, 295, and 370 nm. IX was detected in 12 beer samples and the total levels of (+)- and (-)-IX ranged from 0.15 to 1.4 mg/L. But the amount of xanthohumol (XN) was below the detection limit (0.017 mg/L). Each level of (-)-IX was almost the same as that of (+)-IX, suggesting that IX was a racemic mixture in these beer samples. The racemization of IX in beer could be attributed to the production of a racemic mixture from XN during boiling and to the fact that IX enantiomers were easily interconverted. 相似文献
75.
Atsushi Ikegaya Tomoaki Kawata Toru Ikari Yuji Emoto Yosuke Sato Takashi Takeuchi 《Soil Science and Plant Nutrition》2020,66(3):449-457
ABSTRACT The fertilizer absorption characteristics of strawberries are not clear, although appropriate fertilization is definitely necessary to ensure produce quality and quantity. This study aimed to determine the amounts of macro- and micronutrients absorbed during cultivation of strawberries and their biodistribution and utilization in the plant body. We cultivated Japanese strawberries ‘Benihoppe’ and ‘Kirapika’ in small hydroponic equipment containing a nutrient solution and determined the amounts of N, P, K, Ca, Mg, Fe, Mn, B, Zn, Cu, and Mo absorbed during and at the end of cultivation. The results revealed the adsorption levels of these elements during the cultivation period. The nutrient concentrations varied greatly among plant organs. In particular, P and B accumulated at high levels in the leaves and stem, K, Ca, Mg, Mn, Zn, and Cu accumulated in the crown, and N, Fe, and Mo accumulated in the roots. In addition, the uptake levels of N, P, K, Mg, Mn, Zn, and Cu differed between Benihoppe and Kirapika. Our results provide useful information for determining fertilizer application rates in strawberry cultivation. 相似文献
76.
Hitoshi Fujiie Atsushi Maruyama Masako Fujiie Michiko Takagaki Douglas J. Merrey Masao Kikuchi 《Irrigation and Drainage Systems》2011,25(1):39-60
It is well-known that major irrigation projects have a strong scale economy, handicapping irrigation development in sub-Saharan
Africa (SSA) because of the difficulty in formulating large-scale projects. Using project-level investment cost and performance
data of major and minor irrigation projects, this paper examines the causes of the economy of scale phenomenon. We find that
strong scale economy exists not only for major but also for minor projects, i.e., small- and micro-scale, projects. This is
largely because of the existence of indivisible overhead costs such as high-opportunity-cost human resources for planning,
designing and engineering management and supervision. We also find that large differences between major and minor projects
in the absolute level of overhead as well as construction costs creates a strong scale diseconomy and results in better performance
of minor projects. The advantage of minor projects holds even when their higher risk associated with the water source is taken
into consideration. We argue that there is an urgent need to promote irrigation development in SSA through developing minor
projects, and to reduce the heavy burden of overhead costs by developing the capacity of human resources at the national,
local and farmer levels in the fields of irrigation engineering, irrigation agronomy, institutional development, and micro
water management technologies. 相似文献
77.
78.
79.
Shun-ichiro Takano Atsushi Mochizuki Keiji Takasu Kazuhiko Konishi Jelfina C. Alouw Donata S. Pandin Satoshi Nakamura 《Journal of pest science》2013,86(2):151-155
Brontispa longissima (Coleoptera: Chrysomelidae) is a serious invasive pest of coconut palm (Cocos nucifera) supposedly originating from Indonesia and New Guinea. It has recently invaded Southeast and East Asia, where it has caused serious damage to coconut plants. Brontispa longissima as currently defined contains two cryptic species: we herein referred to one as the “Asian clade”, which is distributed over a wide area, including Asia and the Pacific region; and we referred to the other one as the “Pacific clade”, which is found in a limited area in the Pacific region. We developed a PCR–RFLP method for differentiating the two clades. Digestion of the PCR product of a 1,014-bp region within the mitochondrial cytochrome oxidase I gene (COI) with BslI, HpyCH4III, or NlaIV resulted in clade-specific patterns as estimated by the sequence data. We applied the method to specimens newly obtained from various locations to investigate the geographical distribution of B. longissima. Although B. longissima collected from Samoa in April 2003 had been placed in the Pacific clade, specimens collected from the same island in April 2010 were placed in the Asian clade, suggesting that the predominant clade may have been changing from the former to the latter. On Timor, specimens included both clades in apparently segregated habitats. 相似文献
80.
Hajime Ozawa Atsushi Watanabe Kentaro Uchiyama Yoko Saito Yuji Ide 《Journal of Forest Research》2012,17(6):488-498
We attempted to evaluate the genetic diversity of long-distance transported pollen flowing over fragmented Pinus densiflora populations during a mating season. A P.?densiflora clonal seed orchard, which was located in a rural area where many fragmented populations exist, was selected for pollen capture. Immigrant pollen captured by three clones having different flowering times was regarded as the pollen flowing over fragmented populations during a mating season. The genetic diversity (H e) values of the immigrant pollen captured by the three clones were high (H e?>?0.894). The correlation of paternity (r p) values of the seeds having immigrant parent generated from the three clones were calculated to be negative. From these parameters, the pollen cloud is considered to have maintained high genetic diversity during the mating season. The genetic composition of the pollen cloud showed slight variation. The pollen captured by different trees (i.e., clonal ramets of the three clones) was significantly different based on analysis of molecular variance. Especially, the pollen pools captured by trees planted in the western side of the orchard were significantly different from the gene pool of the surrounding populations. Factors affecting this differentiation could be that the donors of the pollen transported to the orchard vary with time, as well as nonuniform dispersal of the pollen. From these results, the pollen flowing over fragmented P.?densiflora populations is considered to have high genetic diversity, compensating to some extent for fragmentation. 相似文献