The effect of GnRH on the pregnancy ratio in low-yielding local race cows: comparison of different injection times

Tropical Animal Health and Production - The objective of this article was to investigate the efficiency of GnRH administrations at different time points after induced luteolysis on pregnancy rates...     

Extraction of light hydrocarbons from soil

The recovery of light hydrocarbon constituents from three soils using three kinds of extraction methods, conducted in duplicates, was evaluated. Higher molecular weight compounds were recovered at greater than 80% whereas the recoveries of benzene, toluene, ethylbenzene, and xylene were generally less than 50%. Using the most efficient extraction procedure, the average recovery of light hydrocarbon from a soil, was 78%. Increased soil moisture content decreased the extent of recovery. Methanol and 2-propanol showed similar extracting potential for light hydrocarbon from soil. The rate of hydrocarbon removal was faster when methanol was used as a solvent, but its efficiency was similar to 2-propanol. The batch and column extraction methods gave very similar results for both solvents. However, column extraction offered an advantage over the soxhlet and batch procedures by reaching equilibrium faster.     

Cattle brucellosis in traditional livestock husbandry practice in Southern and Eastern Ethiopia,and its zoonotic implication

Background

Cattle brucellosis has significant economic and zoonotic implication for the rural communities in Ethiopia in consequence of their traditional life styles, feeding habits and disease patterns. Hence, knowledge of brucellosis occurrence in traditional livestock husbandry practice has considerable importance in reducing the economic and public health impacts of the disease.

Methods

A total of 1623 cattle sera were serially tested using the rose Bengal test as screening and complement fixation test as confirmatory tests. The Stata survey command was used to establish prevalences for the overall and individual variables, while potential risk factors for seropositivity were analyzed using a multivariable logistic regression analysis.

Results

The results showed that 3.5% (95% CI = 2.4, 4.5%) of the animals and 26.1% (95% CI = 18.6, 33.7) of the herds tested had antibodies against Brucella species. Village level seroprevalence ranged from 0% to 100%. A higher seroprevalence was observed in pastoral system than mixed farming although this variable was not significant in the final model. The final logistic regression model identified herd size; with large (odd ratio (OR) = 8.0, 95% CI = 1.9, 33.6) and medium herds (OR = 8.1, 95% CI = 1.9, 34.2) showing higher risk of Brucella infection when compared to small herds. Similarly, the odds of Brucella infection was higher in cattle aged above 4 years when compared to age groups of 1-2 (OR = 5.4, 2.1, 12.9) and 3-4 years (OR = 3.1, 95% CI = 1.0, 9.6). Herd level analysis of the risk factors revealed that large and medium herds as well as herds kept with multiple livestock species were at higher risk of acquiring Brucella infection. Brucellosis in traditional livestock husbandry practices certainly poses a zoonotic risk to the public, in consequence of raw milk consumption, close contact with animals and provision of assistance during parturition. Due to lack of diagnostic facilities and information on its occurrence, human brucellosis is most likely misdiagnosed for other febrile diseases prevailing in the areas and treated empirically.

Conclusions

The results of this study demonstrated that bovine brucellosis is widely prevalent in the study areas particularly in pastoral production system. Hence, the study suggests the need for implementing control measures and raising public awareness on prevention methods of brucellosis.     

Effect of pentoxifylline on endotoxin-induced haemostatic disturbances in rabbits

Pentoxifylline (PTX, a methylxanthine derivative) has been found to interrupt early gene activation for tumour necrosis factor, interleukin-1, interleukin-6 and tissue factor production and to improve survival from experimental sepsis. During endotoxaemia, lipopolysaccharide (LPS, endotoxin) and proinflammatory cytokines trigger the development of disseminated intravascular coagulation (DIC) via the tissue factor-dependent pathway of coagulation. The present study was undertaken to determine whether pentoxifylline could prevent coagulation disturbances in LPS-treated rabbits. Endotoxaemia was induced with E. coli lipopolysaccharide in New Zealand White rabbits. Forty rabbits were used and divided into four equal groups. Group 1 served as a control group; Group 2: lipopolysaccharide was injected intravenously, Group 3: pentoxifylline was injected intraperitoneally, Group 4: lipopolysaccharide and pentoxifylline were injected simultaneously. Blood samples were collected 6 h after the treatments. In rabbits with endotoxin-induced DIC, platelet count, leukocyte count, percentage of differential leukocyte values, fibrinogen level, antithrombin III (AT-III) and protein C (PC) activity were decreased. Moreover, activated partial thromboplastin time (APTT) and prothrombin time (PT) were prolonged when compared to the control group. In conclusion, haemostatic disturbances associated with endotoxin-induced DIC were moderately suppressed by the administration of PTX.     

Imaging and surgical outcomes of spinal tumors in 18 dogs and one cat

Clinical and magnetic resonance imaging (MRI) findings, histological appearances and surgical outcomes of 18 dogs and one cat with spinal tumors are presented. Medical records of the cases admitted for spinal disorders were reviewed, and cases of spinal tumors that were diagnosed by MRI and confirmed by histological examination were included in this study. T1 weighted, T2 weighted and contrast enhanced T1 weighted images were taken and interpreted to evaluate the spinal tumors. The tumors were diagnosed as: meningioma (n = 6), ependymoma (n = 1), nerve sheath tumor (n = 4), metastatic spinal tumor (n = 3), osteosarcoma (n = 2), osteoma (n = 1), rhabdomyosarcoma (n = 1), and nephroblastoma (n = 1). Thirteen cases underwent surgical operation and the remaining six cases were euthanized at the request of the owners. The neurological status of the surgical cases did not deteriorate, except for one dog that showed ependymoma in the early period after the operation. These results indicate the potential for surgical gross total tumor removal of vertebral tumors to provide better quality of life and surgical collection of histological specimens for definitive diagnosis. For effective case management, dedicated MRI examination is important to accurate evaluation of the spinal tumors, and surgical treatment is useful for extradural and intradural-extramedullary spinal tumors.     

Performance of Arsi-Bale sheep fed urea treated maize cob as basal diet and supplemented with graded levels of concentrate mixture

The experiment was conducted using 20 yearling intact male Arsi-Bale sheep with a mean body weight of 20.56?±?0.45 (mean?±?SD) to investigate the performance and economic benefit of graded levels of concentrate supplementation to urea-treated maize cob (UTMC)-based diet. The experiment consisted of 7 days of digestibility trial and subsequent 90 days of feeding trial. Before the commencement of the experiment, sheep were vaccinated against common infectious diseases, dewormed, and disinfected against internal and external parasites, respectively. The experimental design was randomized complete block design. The sheep were grouped into five blocks of four animals based on their initial body weight, and one animal from each block was randomly assigned to one of the four treatments. The treatments included ad libitum feeding of UTMC (T1) and supplementation with a concentrate mixture composed of wheat bran (WB) and noug seed cake (NSC) in 2:1 ratio, which were offered at 150 g (T2), 250 g (T3), and 350 g (T4) DM/head/day. The CP content of the UTMC was 9.3% on DM basis. The daily DM intake of UTMC was higher (P?<?0.001) for T4 (614.6 g) compared to T1 (505.1 g), T2 (538.9 g), and T3 (590.3 g). Total DM intake was higher (P?<?0.001) for supplemented treatments T2 (688.4 g), T3 (844.1 g), and T4 (966.9 g). Supplementation did not improve (P?>?0.05) digestibility of DM, OM, NDF, and ADF, except CP digestibility which was significant (P?<?0.001) across treatments. Supplementation increased (P?<?0.001) final body weight (FBWT), feed conversion efficiency (FCE), and average daily weight gain (ADG). The average daily gain (ADG) was highest (P?<?0.001) in T4 (80.8?±?7.74 g/day) followed by T3 (33.3?±?7.74 g/day) and T2 (23.1?±?0.95 kg) whereas the un-supplemented animals lost about 9.2 g/day. The highest total return, net income, and marginal rate of return were observed in higher level supplemented sheep than the control. Therefore, T4 improved biological performance and marginal rate of return than the other treatments and can be considered as better alternative feed supplement in UTMC-based feeding of Arsi-Bale sheep in Ethiopia.     

Seroprevalence and factors associated with bovine viral diarrhea virus (BVDV) infection in dairy cattle in three milksheds in Ethiopia

This work was conducted to estimate the seroprevalence, to identify potential factors that influence seroprevalence of bovine viral diarrhea virus (BVDV), and to investigate the association between BVDV serostatus and occurrence of reproductive disorders in dairy cattle in three milksheds in Ethiopia. A total of 1379 serum samples were obtained from cattle randomly selected from 149 herds from three milksheds representing central, southern, and western Ethiopia. Sera samples were examined for bovine viral diarrhea virus (BVDV) antibodies using commercial competitive enzyme-linked immunosorbent assay (ELISA) kit. Logistic regression analysis was employed to investigate associations between risk factors and the risk of BVDV seroprevalence, and BVDV serostatus and reproductive disorders. Seroreaction to BVDV antigens was detected in 32.6% of the 1379 cattle and 69.8% of the 149 herds sampled. Factors associated with BVDV seroplevalence were age, breed, and herd size (P?<?0.05). Adult cattle ≥?18 months old had 2.1 (95% CI 1.5, 3.1) times the odds of BVDV seroreaction than younger cattle. Holstein-Friesian (HF) local crosses (OR?=?2.1, 95% CI 1.3, 3.4) and HFs (OR?=?1.3, 95% CI 0.9, 1.9) were more likely to be seropositive than Jersey and the odds of seropositivity in cattle in large herds with 11 or more animals were higher (OR?=?1.8, 95% CI 1.3, 2.5) than the odds of BVDV seropositivity in smaller herds. Seroprevalence was not associated with geographical region (P?>?0.05). Risk of reproductive disorders was not affected by BVDV serostatus, except for repeat breeding (P?>?0.05). The present study demonstrated that BVDV has wide distribution in the country being detected in all the 15 conurbations and 69.8% of herds involved in the study.     

On farm evaluation of sun-dried Ziziphus spina-christi leaves substitution for natural pasture hay on feed intake and body weight change of Bati goat breeds in Ethiopia

The study was conducted to evaluate the effect of feeding different levels of sun-dried Ziziphus spina-christi (ZC) leaves on feed intake and live weight gain of local male goats. Twenty-five male local breed of goats at 1 year of age (body weight; BW?=?20.7?±?0.72 kg) were randomly allocated to five treatments and five replications. Treatments were as follows: T1 (grass hay only; control group); T2 (75% grass hay + 25% ZC leaves); T3 (50% grass hay + 50% ZC leaves); T4 (25% grass hay + 75% ZC leaves), and T5 (100% ZC leaves). The study lasted for 90 days. During this period, feed intake, BW change, and average daily gain were evaluated. There was a significant difference in total dry matter intake of goats fed on different levels of ZC leaves compared with the control group. Mixing of the pasture grass hay diet improved (P?<?0.05) goats feed intake from 440 to 592 g/head/day at the minimum proportion of 25% ZC and natural pasture mixture. However, there was no significant difference (P?>?0.05) in feed intake among the supplemented groups (T2–T5) as ZC leaves proportion increased. Goats in the control group and in the 25% ZC treatment diet gained 31.4 and 46.7 g, which was not different (P?>?0.05). The highest average daily BW was gained (P?<?0.05) in goats supplemented with the highest level (100%) of ZC leaves. T5 (100% ZC leaves supplementation) showed a significant increment (P?<?0.05) in body weight gain of goats over other treatments and the control. Feed conversion ratio was increased (P?<?0.05) with increased inclusion level of ZC leaves. Goats on supplemented treatments 1 and 2; treatments 4 and 5 had no significant difference (P?>?0.05) in feed conversion efficiency while there was a significant (P?<?0.05) difference between treatments 2 and 3 diets which had higher (P?<?0.05) feed conversion ratio than the control groups. Generally, the results of the current study showed that supplementation of goats with above 50% level of dried ZC leaves in their diet significantly increased feed intake and body weight gain by improving feed conversion efficiency of goats.