全文获取类型
收费全文 | 2545篇 |
免费 | 145篇 |
专业分类
林业 | 214篇 |
农学 | 306篇 |
基础科学 | 32篇 |
584篇 | |
综合类 | 124篇 |
农作物 | 198篇 |
水产渔业 | 301篇 |
畜牧兽医 | 649篇 |
园艺 | 72篇 |
植物保护 | 210篇 |
出版年
2023年 | 36篇 |
2022年 | 73篇 |
2021年 | 82篇 |
2020年 | 102篇 |
2019年 | 122篇 |
2018年 | 152篇 |
2017年 | 156篇 |
2016年 | 121篇 |
2015年 | 88篇 |
2014年 | 118篇 |
2013年 | 234篇 |
2012年 | 183篇 |
2011年 | 161篇 |
2010年 | 137篇 |
2009年 | 93篇 |
2008年 | 106篇 |
2007年 | 98篇 |
2006年 | 86篇 |
2005年 | 60篇 |
2004年 | 53篇 |
2003年 | 43篇 |
2002年 | 30篇 |
2001年 | 40篇 |
2000年 | 36篇 |
1999年 | 25篇 |
1998年 | 18篇 |
1997年 | 15篇 |
1996年 | 10篇 |
1995年 | 6篇 |
1994年 | 7篇 |
1993年 | 6篇 |
1992年 | 12篇 |
1991年 | 14篇 |
1990年 | 13篇 |
1989年 | 10篇 |
1988年 | 11篇 |
1987年 | 12篇 |
1986年 | 7篇 |
1985年 | 6篇 |
1984年 | 7篇 |
1983年 | 8篇 |
1982年 | 5篇 |
1981年 | 9篇 |
1980年 | 5篇 |
1979年 | 14篇 |
1974年 | 8篇 |
1973年 | 8篇 |
1971年 | 7篇 |
1969年 | 6篇 |
1967年 | 4篇 |
排序方式: 共有2690条查询结果,搜索用时 15 毫秒
11.
Pantu Kumar Roy Ahmad Yar Qamar Xun Fang Ghangyong Kim Seonggyu Bang Mahanama De Zoysa Sang Tae Shin Jongki Cho 《Reproduction in domestic animals》2021,56(2):342-350
Oxidative stress is inevitable as it is derived from the handling, culturing, inherent metabolic activities and medium supplementation of embryos. This study was performed to investigate the protective effect of chitosan nanoparticles (CNPs) on oxidative damage in porcine oocytes. For this purpose, cumulus–oocyte complexes (COCs) derived from porcine slaughterhouse ovaries were exposed to different concentrations of CNPs (0, 10, 25 and 50 µg/ml) during in vitro maturation (IVM). Oocytes treated with 25 µg/ml CNPs showed significantly higher levels of GSH, along with a significant reduction in ROS levels compared to control, CNPs10 and CNPs50 groups. In parthenogenetic embryo production, the maturation rate was significantly higher in the CNPs25 group than that in the control and all other treated groups. In addition, when compared to the CNPs50 and control groups, CNPs25-treated oocytes showed significantly higher cleavage and blastocyst development rates. The highest concentration of CNPs reduced the total cell number and ratio of ICM: TE cells in parthenogenetic embryos, suggesting that there is a threshold where benefits are lost if exceeded. In cloned embryos, the CNPs25 group, as compared to all other treated groups, showed significantly higher maturation and cleavage rates. Furthermore, the blastocyst development rate in the CNPs25-treated group was significantly higher than that in the CNPs50-treated group, as was the total cell number. Moreover, we found that cloned embryos derived from the CNPs25-treated group showed significantly higher expression levels of Pou5f1, Dppa2, and Ndp52il genes, compared with those of the control and other treated groups. Our results demonstrated that 25 µg/ml CNPs treatment during IVM improves the developmental competence of porcine oocytes by reducing oxidative stress. 相似文献
12.
Land degradation is a global problem. Best management of degraded land can be done by evaluating the spatial variability of soil properties including chemical properties of degraded land and mapping such variations. Since, a significant portion of arable land in India is chemically degraded due to soil acidity; the present study was conducted to study the spatial variability of soil acidity (pH), electrical conductivity (EC), soil organic carbon (OC) content, exchangeable potassium (K+), calcium (Ca2+) and magnesium (Mg2+) contents in some cropped acid soils of India. A total of four hundred (one hundred from each series) representative surface (0–0.15 m depth) soil samples were collected from arable soils representing four soil series namely Hariharapur, Debatoli, Rajpora and Neeleswaram situated in Orissa, Jharkhand, Himachal Pradesh and Kerala states of India, respectively, and were analyzed. Soil acidity (pH between 3.90 and 6.45) showed a low variability, in contrast to other soil properties, which showed moderate variability. The coefficients of variation varied from 32.4 to 74.3, 31.2 to 50.9, 45.6 to 100, 71.9 to 93.0 and 59.0 to 79.8% for EC (mean between 0.05 and 0.09 dS m−1), OC (mean between 0.29 to 1.86%), exchangeable K+ (mean between 39.1 and 77.7 mg kg−1), Ca2+ (mean between 148 and 293 mg kg−1) and Mg2+ (mean between 111 and 191 mg kg−1), respectively. Soil pH and OC content were positively and significantly correlated with exchangeable K+, Ca2+ and Mg2+ content. Geostatistical analysis revealed that the best fit models were gaussian, exponential and spherical for different soil properties with moderate to strong spatial dependency. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
13.
Identification of internal control genes in milk‐derived mammary epithelial cells during lactation cycle of Indian zebu cow 下载免费PDF全文
14.
15.
16.
Kumar Sandopu Sravan Arya Monisha Nagbhushan Priyanka Giridhar Parvatam Shetty Nandini P. Yannam Sudheer Kumar Mahadevappa Paramesha 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(2):283-291
Plant Foods for Human Nutrition - The aim of this study was to assess the efficiency of different drying methods (room, sun, oven, microwave, cross-flow, infra-red, dehumidifier, and freeze-drying)... 相似文献
17.
Heat shock protein 70 expression in different tissues of Cirrhinus mrigala (Ham.) following heat stress 总被引:2,自引:0,他引:2
Members of heat shock protein 70 (HSP70) are highly conserved proteins of about 70 kDa and play important roles in protein folding. Levels of these proteins increase when cells are under stress. Environmental temperature influences both the basal and induced levels of HSPs. However, studies on HSPs in fishes from a tropical country such as India are lacking. In the present study, Indian major carp (IMC) Cirrhinus mrigala (Ham.) acclimatized at 25±2°C had high levels of HSP70, viz., 1.2–1.3 ng μg?1 total protein in kidney and gill and 4.2–5.3 ng μg?1 total protein in liver and brain tissues, indicating the presence of biochemically significant levels of stress. However, maintenance of acclimatized fish at 17°C for up to 48 h did not lead to a significant decrease in stress protein levels. A heat shock at 37°C for up to 48 h resulted in only two to threefold increase in HSP70 levels in these organs. Although the increase in HSP70 levels was apparent from the first hour of heat stress in all these tissues, the increase was significant from the second hour in the brain, the sixth hour in liver and kidney and the 20th hour in the gills. 相似文献
18.
Sujata Mohapatra Rajesh Kumar Srianga Tilak Patnaik Choudhury Suryakant Mishra Lakshman Sahoo Jitendra Kumar Sundaray 《Aquaculture Research》2020,51(8):3230-3236
Monocrotophos (MCP) is one of the organophosphate pesticides extensively used in agriculture and animal husbandry. The present study reports the effects of sub‐lethal concentrations of MCP on gonadosomatic index (GSI), gonadal histology and breeding fitness of the fish Anabas testudineus. The fish were exposed to three sub‐lethal concentrations of monocrotophos (T1: 3.5 mg/L, T2: 5.3 mg/L and T3: 10.6 mg/L) for 45 days with 12 fish (males and females in equal numbers) in each tank, and one group was kept as control (C) without any treatment. GSI decreased in all treatment groups in comparison with control. Significant decrease in fertilization and hatching rate was observed at all concentrations in comparison with control on 15, 30 and 45 days of monocrotophos exposure. The decreased vitellogenesis, disruption of follicular wall and oocyte atresia were observed in all the treated females at 30 and 45 days of exposure. Rupture of seminiferous tubules was noticed in all the treated males irrespective of exposure periods. The results thus indicated that monocrotophos could deleteriously impact the gonadal structure and function in A. testudineus, which could seriously impact the reproductive success of the animal. 相似文献
19.
Swagatika Sahu Basanta Kumar Das Bibhudendu Kumar Mishra Jyotirmayee Pradhan Surya Kanta Samal Niranjan Sarangi 《Aquaculture Research》2008,39(16):1720-1730
The aim of this study was to evaluate the dietary dosages of turmeric that enhance immune response and disease resistance against the opportunistic pathogens Aeromonas hydrophila in Labeo rohita fingerlings. Hence, four different dosages of turmeric at 0.1, 0.5, 1.0 and 5.0 g kg?1 feed were given to the fingerlings of L. rohita for 60 days at 4% body weight. At every 20‐day interval, different biochemical, haematological, enzymatic and immunological parameters of fish were evaluated. After 60 days, fish were challenged with A. hydrophila. The mortality (%) was recorded on the tenth day post challenge. Most of the immune parameters including lysozyme activity, superoxide anion production and serum bactericidal activity were significantly (P<0.05) higher on 60 days of feeding of 1.0 g of turmeric per kg of feed. Challenge study indicated 100% and 89% survivability in the group of fish fed with 5.0 and 1.0 g of turmeric per kg of feed respectively. Feeding of turmeric might have maintained long‐term protection in fish by elevating the nonspecific immune system such as Nitroblue tetrazolium (NBT), lysozyme and serum bactericidal activity. The result showed that turmeric at a dose of 1.0 g kg?1 feed for 60 days provided the greatest protection to pathogen challenge. 相似文献
20.
R. Ramesh Kumar L. Prasanna Anjaneya ReddyJ. Vinay Kumar K. KomaraiahS. Purnanand K.P. Sastry 《Industrial Crops and Products》2012,35(1):199-202
Ashwagandha (Withania somnifera) is an important medicinal plant and its dried roots are used in traditional systems of medicine. The market price of roots is determined by physical (textural) quality. Brittle roots with high starch and low fiber are considered to be superior because of ease in grinding. Genetic diversity studies based on root textural parameters have not been done so far. So the present study was designed to assess genetic diversity for morphometric traits and root textural quality parameters among two morphologically distinct groups: Poshita and Nagore. The PCA separated the morphometric and root texture variables distinctly into two different principal components: PC-1 and PC-2 respectively, indicating that both are negatively associated. All the morphotypes in Poshita group showed high positive loadings in PC-1 indicating that component genotypes are high root yielding. Nagore morphotypes were low yielding but the root texture was good. Clustering of morphotypes grouped Poshita and Nagore separately with high inter-cluster distances indicating that both groups are highly divergent from each other, suggesting that there is sufficient scope for varietal improvement through hybridization. 相似文献