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81.
Toshie ISERI Junichiro SHIMIZU Hideo AKIYOSHI Kayo KUSUDA Akiyoshi HAYASHI Keiichiro MIE Takeshi IZAWA Mitsuru KUWAMURA Jyoji YAMATE Yuka FUJIMOTO Fumihito OHASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):857-860
A Bengal tiger was presented for evaluation of weakness, ataxia and inappetance. Computed
tomography (CT) and magnetic resonance imaging (MRI) revealed a mass extending from the
T7-8 vertebral body to the left rib and compressing the spinal cord. On CT, the bone
destruction and sequestrum were shown. On MRI, the multilobulated mass appeared hypo- to
isointense in T1-weighted and hyperintense in T2-weighted images. The tiger died after
imaging, most likely from renal failure. Chordoma without metastasis was diagnosed on
necropsy. The imaging characteristics were similar to those found in chordoma in humans.
This report describes the use of CT and MRI in an exotic species. 相似文献
82.
Yuka Onozawa Masaaki Chiwa Hikaru Komatsu Kyoichi Otsuki 《Journal of Forest Research》2009,14(2):111-116
In recent years, moso bamboo (Phyllostachys pubescens) forest areas in Japan have rapidly expanded, and bamboo is now invading nearby natural or plantation forests. To date, only
one study has examined the rainfall interception of a moso bamboo forest. In that study, it was reported that the interception
ratio (interception/rainfall) of the bamboo forest did not exceed the interception ratios of other natural and plantation
forests (n = 4) in Japan. To expand the current state of knowledge about rainfall interception of bamboo forests, we measured throughfall
and stemflow at another bamboo forest site. Annual rainfall (Rf), throughfall (Tf), and stemflow (Sf) during the measurement period were 2,105, 1,556, and 322 mm, respectively. Annual rainfall interception at the plot (I) was 228 mm. Tf/Rf, Sf/Rf, and I/Rf were 73.9, 15.3, 10.8%, respectively. I/Rf was less than 20% throughout the year except in October, the month with lowest rainfall. We also summarized rainfall interception
data from 19 other natural and plantation forests. The I/Rf value of our site did not exceed the I/Rf values of these natural and plantation forests (n = 19). Our data will be useful for assessing changes in water resources that result from replacement of natural or plantation
forests by bamboo forests. 相似文献
83.
Degradation of high-molecular-weight polyethylene membrane by lignin-degrading fungi, IZU-154, Phanerochaete chrysosporium, and Trametes versicolor, was investigated under various nutritional conditions. IZU-154 showed the most significant polyethylene degradation among the three lignin-degrading fungi under nitrogen- or carbon-limited culture conditions. Furthermore, for T. versicolor and P. chrysosporium, the addition of Mn(II) into nitrogen- or carbon-limited culture medium enhanced polyethylene degradation. These results suggest that polyethylene degradation is related to ligninolytic activity of lignin-degrading fungi. Treatment of polyethylene membrane with partially purified manganese peroxidase (MnP) caused significant degradation in the presence of Tween 80, Mn(II), and Mn(III) chelator. This result demonstrates that MnP is the key enzyme in polyethylene degradation by lignin-degrading fungi.This study was presented in part at the 9th International Symposium on Wood and Pulping Chemistry, Montreal, Canada, June 9–12, 1997 相似文献
84.
Yosuke Tanaka Hiroshi Minami Yuka Ishihi Kazunori Kumon Kentaro Higuchi Takeshi Eba Akefumi Nishi Hideki Nikaido Satoshi Shiozawa 《Aquaculture Research》2014,45(3):537-545
In mass culture of Pacific bluefin tuna Thunnus orientalis, a marked growth variation is observed after they start feeding at 6–7 mm in body length (BL) on yolk‐sac larvae of other species, and the growth variation in tuna larvae is a factor leading to the prevalence of cannibalism. To examine the relationship between prey utilization and growth variation, nitrogen stable isotope ratios (δ15N) of individual larvae were analysed. A prey switch experiment was conducted under two different feeding regimes: a group fed rotifers (rotifer fed group), and a group fed yolk‐sac larvae of spangled emperor, Lethrinus nebulosus (fish fed group) from 15 days after hatching (6.87 mm BL). The fish fed group showed significantly higher growth than the rotifer fed group. Changes in the δ15N of the fish fed group were expressed as an exponential model and showed different patterns from those of the rotifer fed group. The δ15N of fast‐growing tuna larvae collected in an actual mass culture tank after the feeding of yolk‐sac larvae was significantly higher than those of the slow‐growing larvae, indicating that slow glowing larvae depended largely on rotifers rather than the yolk‐sac larvae. 相似文献
85.
Sugiyama A Sun J Nishinohara M Fujita Y Masuda A Ochi T Takeuchi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(6):821-826
The purpose of this study was to evaluate whether N(ε)-hexanoyl lysine (N(ε)-HEL) and acrolein reflect the severity of cisplatin-induced nephrotoxicity. Immunoexpression of N(ε)-HEL and acrolein in kidneys and their urinary concentration were examined up to day 4 post-cisplatin injection in rats. Cisplatin-induced tubular injury was observed histopathologically on days 2-4 after injection and became more severe time-dependently. On days 2-4, N(ε)-HEL and acrolein were immunostained in the cytoplasm of damaged tubular cells. Their immunostaining intensity and urinary levels increased as tubular injury became more severe. These results suggest that expressions of N(ε)-HEL and acrolein were associated with the pathogenesis of cisplatin-induced nephrotoxicity. 相似文献
86.
Matsuura K Kuroda Y Yamashita K Funahashi H 《The Journal of reproduction and development》2011,57(1):163-167
Motile porcine sperms adhere to hydrophilic materials such as glass and plastics. The adsorption of sperms to a hydrophobic poly(dimethylsiloxane) (PDMS) membrane is less compared with that to glass. We investigated the linear velocity (LV) and amplitude of lateral head displacement (ALHD) of motile porcine sperm on glass and PDMS preparations using computer-assisted sperm analysis (CASA). Significant decreases were observed in the 15-min LV (P<0.05) and ALHD (P<0.05) in motile porcine sperm on glass preparations compared with those on PDMS preparations. These differences were due to adsorption of the head and/or neck to hydrophilic substrates. Because of the elasticity of PDMS, we propose that a PDMS membrane should be used for CASA. To investigate the dynamics of motile porcine sperms with microfluidics, we do not recommend plasma treatment to bond PDMS and glass in the microchannel preparation; instead, we suggest that a PDMS molding process without plasma treatment be used for preparation of microfluidic channels. 相似文献
87.
Lee SH Ichii O Otsuka S Hashimoto Y Namiki Y Kon Y 《The Japanese journal of veterinary research》2011,59(2-3):79-88
MRL/MpJ (MRL) is a mouse model for autoimmune disease and develops ovarian cysts with age. The ovarian cysts originate from the rete ovarii, which is considered to be the remnant of fetal mesonephric tubules. In a previous study, we analyzed the genetic background of ovarian cysts by using backcross progenies between MRL and C57BL/6N (B6) mice. By interval mapping, suggestive linkages were detected on several chromosomes (Chrs), and a significant linkage on Chr 14 was designated as MRL Rete Ovarian Cyst (mroc). In the present study, which evaluated 113 F2 intercross progenies, a significant linkage appeared on Chr 6 at the marker position D6Mit188 (likelihood ratio statistic = 18.5). In particular, the peak regions of Chrs 6 and 14, which contain major causative loci by backcross analysis, showed close reverse interaction. From these results, a locus on Chr 6 was identified as mroc2, the second major locus associated with ovarian cyst formation in MRL mice. 相似文献
88.
89.
Shotarou Nukui Satomi Kitamura Tomoyo Hioki Hideaki Ootsuka Kazumitsu Miyoshi Takao Satou Yuka Takatori Tomo Oomiya Keiichi Okazaki 《Breeding Science》2011,61(4):327-337
Fertile plants undergoing male gametogenesis can be treated with nitrous oxide (N2O) gas to obtain 2n male gametes. N2O treatment is also expected to restore the fertility of interspecific hybrids through meiotic restitution or mitotic amphidiploidization. However, this technique has few applications to date, and it is un-known how N2O treatment restores fertility in sterile hybrids. To establish optimal N2O treatment conditions and determine its cytological mechanism of action, we treated various sized floral buds with N2O gas at different anther developmental stages from fertile and sterile hybrid lilies. N2O treatment using the optimal 1–4 mm floral buds induced mitotic polyploidization of male archesporial cells to produce 2n pollen in fertile hybrid lilies. In sterile hybrid lilies, N2O treatment doubled the chromosome number in male archesporial cells followed by homologous chromosome pairing and normal meiosis in pollen mother cells (PMC), resulting in restoration of pollen fertility. Backcrossing the resultant fertile pollen to Lilium × formolongi produced many triploid BC1 plants. Thus N2O treatment at the archesporial cell proliferating stage effectively overcame pollen sterility in hybrid lilies, resulting in fertile, 2n pollen grains that could produce progeny. The procedure presented here will promote interspecific or interploidy hybridization of lilies. 相似文献
90.
进行了臂形草内生菌特异DNA片段的克隆及分子鉴定方法的研究。从5个臂形草品种分离的5个内生菌纯培养特中,提取基因组DNA,通过140条随机引物的RAPD分析,引物OPAK10扩增出1条约500bp的共有PCR谱带。此DNA片段命名为BE1。对BE1进行分离、纯化,经点杂交证实BE1为臂形草内生菌特异DNA片段。进一步将BE1片段进行回收、克隆和DNA序列分析。BE1在基因库中进行序列分析,未发现相关同源片段。臂形草内生菌特异DNA片段的获得,为建立一种准确、快速检测臂形草内生菌及特异内生菌方法奠定了分子生物学基础。 相似文献