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101.
102.
María del Mar Alguacil Fuensanta Caravaca Gisela Díaz Purificacin Marín Antonio Roldn 《植物养料与土壤学杂志》2004,167(5):637-644
A field experiment was carried out to evaluate the effectiveness of mycorrhizal inoculation with three arbuscular mycorrhizal (AM) fungi (Glomus intraradices Schenck & Smith, Glomus deserticola (Trappe, Bloss. & Menge), and Glomus mosseae (Nicol & Gerd.) Gerd. & Trappe) and the addition of composted sewage sludge (SS) with respect to the establishment of Retama sphaerocarpa L. seedlings, in a semiarid Mediterranean area. Associated changes in soil chemical (nutrient content and labile carbon fractions), biochemical (enzyme activities), and physical (aggregate stability) parameters were observed. Six months after planting, both the addition of composted SS and the mycorrhizal‐inoculation treatments had increased total N content, available‐P content, and aggregate stability of the soil. Values of water‐soluble C and water‐soluble carbohydrates were increased only in the mycorrhizal‐inoculation treatments. Rhizosphere soil from the mycorrhizal‐inoculation treatments had significantly higher enzyme activities (dehydrogenase, protease‐BAA, acid phosphatase, and β‐glucosidase) than the control soil. In the short‐term, mycorrhizal inoculation with AM fungi was the most effective treatment for enhancement of shoot biomass, particularly with G. mosseae (about 146% higher with respect to control plants). The addition of the composted SS alone was sufficient to restore soil structural stability but was not effective with respect to improving the performance of R. sphaerocarpa plants. 相似文献
103.
Carlos Antonio F. Santos 《Genetic Resources and Crop Evolution》1999,46(5):455-460
The umbu tree (Spondias tuberosa) is a xerophytic woody species endemic to the northeast of Brazil. The present work reports a three-year in situ evaluation of major yield components in a random set of trees in Petrolina city, Pernambuco State, Brazil and the application of repeatability coefficient in the context of genetic resources utilization. Five traits were assessed: total fruit yield per plant (FY); total number of fruits per plant (NF); average fruit weight (FW); average pulp weight (PW) and average fruit diameter (FD). The values observed for FY ranged from 4.2–184 kg with mean of 61.5 kg. The values for NF ranged from 257 to 12,981 fruits/tree with mean 3,993 fruits. For FW, the range was from 10.82–23.36 g with mean of 16.03 g. The values for PW ranged from 7 to 17 g with mean of 11.2 g, while FD values varied from 2.5 to 3.5 cm with mean of 3.0 cm. Six methods were employed to estimate repeatability coefficient (r) for all parameters. The r ranged from 0.68–0.76 for NF; 0.87–0.89 for FW; 0.65–0.75 for FY; 0.64–0.78 for PW and 0.70–0.84 for FD. Narrower ranges across methods were observed to r-values greater than 0.80, as observed for FW and FD. Three to four years of measurements will be necessary for FY, NF, and PW and one year for FW to obtain a precision of 90% to provide a reliable identification of individuals for in situ or ex situ conservation. 相似文献
104.
Measurement of coupled nitrification-denitrification in paddy fields affected by Terrazole,a nitrification inhibitor 总被引:2,自引:0,他引:2
David?CarrascoEmail author Eduardo?Fernández-Valiente Yoanna?Ariosa Antonio?Quesada 《Biology and Fertility of Soils》2004,39(3):186-192
Coupled nitrification-denitrification and potential denitrification were measured as 15N2O and 15N2 evolution rates in ammonium sulphate-treated rice soils with or without Terrazole [5-ethoxy-3 (trichloromethyl) 1,2,3 Thiadizole] under laboratory and field conditions. The greatest coupled nitrification-denitrification activity was found after drying and rewetting the soil, with maximum values of 322 ng N cm–2 h–1 in the laboratory and 90.8 ng N cm–2 h–1 in the field. These 15N2O + 15N2 evolution rates were about 10 times lower than potential denitrification in these soils. These results and the observed decrease in 15N2O + 15N2 evolution rate in soils treated with Terrazole (60% under laboratory conditions and 52% under field conditions) indicate that denitrification was limited by coupled nitrification-denitrification activity. Oxygen and previous addition of ammonium sulphate appear to control the rate of 15N2O + 15N2 evolution in ammonium sulphate-fertilised soils. 相似文献
105.
To verify the presence of glycolaldehyde in wine resulting from reduction of glyoxal after malolactic fermentation, sterilized solutions of synthetic cultures were inoculated with a lactic bacterium of the type Oenococcus oeni. Fermentation was also carried out on solutions with glyoxal added. The resulting glycolaldehyde concentrations turned out to be associated with the amounts of glyoxal present, and glyoxal was seen to decrease as glycolaldehyde increased. In addition, it was observed that glyoxal principally forms from breakdown of sugars and that reduction to glycolaldehyde is mainly promoted by bacterial activity. Finally, the ability of glycolaldehyde to induce browning of (+)-catechin in a model wine system was verified and turned out to be about 10 times higher than that of ascorbic acid. 相似文献
106.
Muegge BD Kuczynski J Knights D Clemente JC González A Fontana L Henrissat B Knight R Gordon JI 《Science (New York, N.Y.)》2011,332(6032):970-974
Coevolution of mammals and their gut microbiota has profoundly affected their radiation into myriad habitats. We used shotgun sequencing of microbial community DNA and targeted sequencing of bacterial 16S ribosomal RNA genes to gain an understanding of how microbial communities adapt to extremes of diet. We sampled fecal DNA from 33 mammalian species and 18 humans who kept detailed diet records, and we found that the adaptation of the microbiota to diet is similar across different mammalian lineages. Functional repertoires of microbiome genes, such as those encoding carbohydrate-active enzymes and proteases, can be predicted from bacterial species assemblages. These results illustrate the value of characterizing vertebrate gut microbiomes to understand host evolutionary histories at a supraorganismal level. 相似文献
107.
Jeffery W. Bentley Robert Tripp Roberto Delgado de la Flor 《Agriculture and Human Values》2001,18(3):319-331
During the 1990s, the Government of Peru began to aggressivelyprivatize agriculture. The government stopped loaning money to farmers' cooperatives and closed the government rice-buying company. The government even rented out most of its researchstations and many senior scientists lost their jobs. As part of this trend, the government eliminated its seed certification agency. Instead, private seed certification committees were set up with USAID funding and technical advise from a US university. The committees were supposed to become self-financing (bycertifying seed grown by small seed producers) and each committee was supposed to encourage the development of a group of small seed-producing firms, clustered around the seedcertification agency. The amazing thing is that many of the seed committees actually accomplished these goals. The agronomists who staffed the committees stood by their jobs,even after US funding ended, even though the committees' income was (at best) modest, and occasionally under the threat of violence from the extreme left. Some seed certificationcommittees failed and others did not. Some of the problems with Peruvian agricultural liberalization can be seen in regard to the seed programs of maize, rice, potatoes, and beans. For example, the government abandoned most research, yet could not resist creating certain distortions in the seed market (e.g.,buying large amounts of seed and distributing them for political ends). 相似文献
108.
109.
A neutralizing antibody selected from plasma cells that binds to group 1 and group 2 influenza A hemagglutinins 总被引:2,自引:0,他引:2
Corti D Voss J Gamblin SJ Codoni G Macagno A Jarrossay D Vachieri SG Pinna D Minola A Vanzetta F Silacci C Fernandez-Rodriguez BM Agatic G Bianchi S Giacchetto-Sasselli I Calder L Sallusto F Collins P Haire LF Temperton N Langedijk JP Skehel JJ Lanzavecchia A 《Science (New York, N.Y.)》2011,333(6044):850-856
The isolation of broadly neutralizing antibodies against influenza A viruses has been a long-sought goal for therapeutic approaches and vaccine design. Using a single-cell culture method for screening large numbers of human plasma cells, we isolated a neutralizing monoclonal antibody that recognized the hemagglutinin (HA) glycoprotein of all 16 subtypes and neutralized both group 1 and group 2 influenza A viruses. Passive transfer of this antibody conferred protection to mice and ferrets. Complexes with HAs from the group 1 H1 and the group 2 H3 subtypes analyzed by x-ray crystallography showed that the antibody bound to a conserved epitope in the F subdomain. This antibody may be used for passive protection and to inform vaccine design because of its broad specificity and neutralization potency. 相似文献
110.
Melosh NA Boukai A Diana F Gerardot B Badolato A Petroff PM Heath JR 《Science (New York, N.Y.)》2003,300(5616):112-115
We describe a general method for producing ultrahigh-density arrays of aligned metal and semiconductor nanowires and nanowire circuits. The technique is based on translating thin film growth thickness control into planar wire arrays. Nanowires were fabricated with diameters and pitches (center-to-center distances) as small as 8 nanometers and 16 nanometers, respectively. The nanowires have high aspect ratios (up to 10(6)), and the process can be carried out multiple times to produce simple circuits of crossed nanowires with a nanowire junction density in excess of 10(11) per square centimeter. The nanowires can also be used in nanomechanical devices; a high-frequency nanomechanical resonator is demonstrated. 相似文献