To date, the mechanisms of inflammation have been poorly studied in fish of commercial interest, due to the lack of development of appropriate experimental models. The current study evaluated a local inflammation triggered by a polymeric carrageenin mixture (a mucopolysaccharide derived from the red seaweed Chondrus crispus) in the skin of gilthead seabream (Sparus aurata). Fish were injected subcutaneously with phosphate-buffered saline (as control) or λ/κ-carrageenin (1%), and skin samples from the injection sites were collected 1.5, 3 and 6 hr post-injection, processed for inclusion in paraplast and stained with haematoxylin–eosin, Alcian blue or periodic acid–Schiff. Furthermore, immunohistochemistry and expression analyses of several cells’ markers and proinflammatory genes were also analysed in samples of the injected sites. Microscopic results indicated an increased number of skin mucus-secreting cells and acidophilic granulocytes in the skin of fish studied at 1.5 hr and 3 hr post-injection with carrageenin, respectively, with respect to the data obtained in control fish. Otherwise, both the gene expression of the non-specific cytotoxic cell marker (granzyme B, grb) and the proinflammatory cytokine (interleukin-1β, il-1β) were up-regulated at 1.5 hr in the skin of fish injected with carrageenin compared with the control fish, whilst the gene expression of acidophilic granulocyte markers (NADPH oxidase subunit Phox22 and Phox40, phox22 and phox40) was up-regulated at 3 and 6 hr in the carrageenin group, compared with the control group. In addition, the gene expression of myeloperoxidase (mpo) was also up-regulated at 6 hr in the skin of fish injected with carrageenin in comparison with control samples. The present results indicate the chronological participation of two important immune cells involved in the resolution of the inflammation in the skin of gilthead seabream. 相似文献
The porbeagle shark Lamna nasus is a large highly migratory shark distributed in cold and temperate marine waters of the North Atlantic and Southern Hemisphere (SH). According to the International Union for Conservation of Nature, the porbeagle is assessed globally as Vulnerable and regionally as Critically Endangered in the North Atlantic and the Mediterranean Sea. This study explored, for the first time, the population genetics of L. nasus at a regional (south-east Pacific Ocean) and global scale.
In this study, the null hypotheses of no genetic discontinuities among populations (i) within the SH, and (ii) between the SH and Northern Hemisphere (NH) were tested. Also, the demographic history of L. nasus in different ocean basins was assessed. Two mitochondrial markers (Control Region [CR] and cox1) well suited for population genetics inferences in sharks were used.
Spatial–genetic analyses suggested two genetic clusters co-occurring in the south-eastern Pacific Ocean. A two-way ANOVA using the cox1 but not the CR mtDNA fragment detected an effect of genetic identity on shark body size. Phylogeographic analyses, haplotype networks, and analyses of molecular variance demonstrated genetic differences between populations from the NH and SH but not among populations in the SH.
Migration estimates indicated limited current maternal gene flow between the two hemispheres but high gene flow within hemispheres. Two well-defined haplotype groups with star-like shapes inhabited all ocean basins in the SH. These results could reflect a historical scenario of reproductive isolation and more recent mixture among previously isolated populations in the SH. A Bayesian skyline plot analysis indicated sudden population expansion in the SH occurring ~100–125 kya.
This study highlights the need for additional studies focusing on the population genomics (using nuclear markers, i.e. single nucleotide polymorphisms) and the general biology of L. nasus to explore the existence of genetically dissimilar populations in the SH. Such studies will help implementing efficient genetic monitoring programmes.
The aim of this study was to perform a morphometric analysis of the different structural tissue layers of the goat stomach to study their prenatal growth from mathematical models fitted to these morphometric data. A total of 90 embryos and fetuses were used, from the early stages of prenatal life until birth. The growth rate of the gastric wall was slower than that of body length; rumen was the stomach compartment displaying slowest growth. In the three non‐glandular compartments, the epithelial layer grew faster than the gastric wall itself, while the growth rate of the abomasal epithelium declined in the early stages of development. A decline in growth rate was also observed for the lamina propria and submucosa in rumen and reticulum from the early embryonic stages, whereas in omasum and abomasum these layers continued to grow as gestation progressed. The tunica muscularis displayed consistent growth in all compartments, growing faster than the gastric wall. Serosa thickness increased as gestation progressed, displaying a decline in growth‐rate only in the omasum. In conclusion, the dynamics of gastric wall growth were governed by the growth rate of each of the component tissue layers. 相似文献
Asobara japonica (Hymenoptera: Braconidae), Ganaspis brasiliensis and Leptopilina japonica (Hymenoptera: Figitidae) are Asian larval parasitoids of spotted wing drosophila, Drosophila suzukii (Diptera: Drosophilidae). This study evaluated these parasitoids’ capacity to attack and develop from 24 non-target drosophilid species. Results showed that all three parasitoids were able to parasitize host larvae of multiple non-target species in artificial diet; A. japonica developed from 19 tested host species, regardless of the phylogenetic position of the host species, L. japonica developed from 11 tested species; and G. brasiliensis developed from only four of the exposed species. Success rate of parasitism (i.e., the probability that an adult wasp successfully emerged from a parasitized host) by the two figitid parasitoids was low in hosts other than the three species in the melanogaster group (D. melanogaster, D. simulans, and D. suzukii). The failure of the figitids to develop in most of the tested host species appears to correspond with more frequent encapsulation of the parasitoids by the hosts. The results indicate that G. brasiliensis is the most host specific to D. suzukii, L. japonica attacks mainly species in the melanogaster group and A. japonica is a generalist, at least physiologically. Overall, the developmental time of the parasitoids increased with the host’s developmental time. The body size of female A. japonica (as a model species) was positively related to host size, and mature egg load of female wasps increased with female body size. We discuss the use of these parasitoids for classical biological control of D. suzukii.
Journal of Pest Science - Formula, Equation 2 and the table 3 are published incorrectly in the original publication of the article. The correct version of the article is given below 相似文献
In several recent reports related to biocatalysis the enormous pool of biodiversity found in marine ecosystems is considered a profitable natural reservoir for acquiring an inventory of useful biocatalysts. These enzymes are characterized by well-known habitat-related features such as salt tolerance, hyperthermostability, barophilicity and cold adaptivity. In addition, their novel chemical and stereochemical characteristics increase the interest of biocatalysis practitioners both in academia and research industry. In this review, starting from the analysis of these featuring habitat-related properties, important examples of marine enzymes in biocatalysis will be reported. Completion of this report is devoted to the analysis of novel chemical and stereochemical biodiversity offered by marine biocatalysts with particular emphasis on current or potential applications of these enzymes in chemical and pharmaceutical fields. The analysis of literature cited here and the many published patent applications concerning the use of marine enzymes supports the view that these biocatalysts are just waiting to be discovered, reflecting the importance of the marine environment. The potential of this habitat should be thoroughly explored and possibly the way to access useful biocatalysts should avoid destructive large-scale collections of marine biomass for enzyme production. These two aspects are day by day increasing in interest and a future increase in the use of marine enzymes in biocatalysis should be expected. 相似文献
The present study evaluated the effect of feed particle size, thermal processing, several levels of fat inclusion and moisture addition on pellet quality and protein solubility in potassium hydroxide (KOH) in a corn, soybean meal and animal by products based broiler diets. The different processing factors were combined in a 2 x 4 x 4 x 2 factorial arrangement in an eight randomized block consisting of eight production series: two particle sizes (coarse: 1,041 microns and medium: 743 microns), four fat inclusion levels at the mixer (15, 25, 35 and 45 g/kg of feed), four moisture addition levels in the conditioner (0, 7, 14 and 21 g/kg of feed) and two thermal processing treatments (conditioner-pellet press treatment or conditioner-expander-pellet treatment) which resulted in 64 different processed feeds. For the determination of the pellet durability index (PDI), the amount of intact pellets and protein solubility determinations, eight feed samples (replicates) were collected for each treatment. The data were transformed using a variation of Box-Cox transformation in order to fit a normal distribution (p 〉 0.05). Adding moisture up to 21 g/kg of feed in the conditioner improved pellet quality of the diets (p 〈 0.05). Expansion of diets before pelleting improved (P 〈 0.05) PDI and amount of intact pellets by 26% and 31%, respectively, as compared to a simple conditioning-pelleting feed processing. Expander treatment (at 110 ℃) decreased (p 〈 0.05) protein solubility in KOH from 686 g/kg to 643 g/kg total protein as compared to pelleting process (at 80-82 ℃). The amount of intact pellets reduced from 773 g/kg to 746 g/kg of feed (/7 〈 0.05) as particle size increased from medium to coarse grinding. Pellet quality was significantly reduced with fat inclusion levels higher than 35 g/kg of diet. 相似文献
The development of a reliable technique to freeze epididymal semen would provide a unique opportunity to preserve valuable genetic material from unexpectedly lost stallions. The aim of this study was to compare the apoptotic indices of sperm obtained from ejaculate, sperm recently recovered from the epididymides (EP), and sperm recovered from epididymides stored at 5°C for 24 hours (EP-stored). For the first category, two ejaculates from seven stallions were collected and then submitted to cryopreservation using an egg yolk-based extender. One week after the last semen collection, the stallions were submitted to bilateral orchiectomy, and sperm from one of the cauda epididymis was harvested immediately after castration (EP). The remaining testicle was stored in a passive refrigeration container at 5°C for 24 hours before the cauda epididymal sperm was harvested (EP-stored). Sperm harvesting from the epididymis for EP and EP-stored was performed by retrograde flushing of the caudal portion of the epididymis using a skim milk-based extender. The recovered sperm was then cryopreserved using the egg yolk-based extender. Sperm motility parameters were studied by computer-assisted semen analysis, and apoptosis was estimated by measuring caspase activity and membrane phospholipid translocation using epifluorescence microscopy. The samples were evaluated immediately (0 hour) and 8 hours after thawing. At 0 hour, no differences in sperm parameters were observed among the groups, but after 8 hours, significant statistical differences were observed in sperm motility parameters and plasma membrane integrity among the treatment groups. In addition, viable cells with no apoptotic signs were more prevalent in EP and EP-stored, suggesting that epididymal sperm is less sensitive to the cold shock caused by sperm cryopreservation. 相似文献
Abstract Francisellosis is an emergent disease in cultured and wild aquatic animals. The causative agent, Francisella noatunensis subsp. orientalis (Fno), is a gram-negative bacterium recognized as one of the most virulent pathogens of warmwater fish. The main objective of this project was to investigate the prevalence of Fno in cultured tilapia (specifically, Mozambique Tilapia Oreochromis mossambicus, Koilapia [also known as Wami Tilapia] O. hornorum, Blue Tilapia O. aureus, and Nile Tilapia O. niloticus hybrids) on the island of Oahu, Hawaii, using conventional and real-time PCR assays followed by statistical modeling to compare the different diagnostic methods and identify potential risk factors. During 2010 and 2012, 827 fish were collected from different geographical locations throughout the island of Oahu. Upon collection of fish, the water temperature in the rearing system and the length of individual fish were measured. Extraction of DNA from different tissues collected aseptically during necropsy served as a template for molecular diagnosis. High correlation between both molecular methods was observed. Moreover, the bacterium was isolated from infected tilapia on selective media and confirmed to be Fno utilizing a species-specific Taqman-based real-time PCR assay. Although a direct comparison of the prevalence of Fno between the different geographical areas was not possible, the results indicate a high prevalence of Fno DNA in cultured tilapia throughout the farm sites located on Oahu. Of the different tilapia species and hybrids currently cultured in Hawaii, Mozambique Tilapia were more susceptible to infection than Koilapia. Water temperature in the rearing systems and fish size also had a strong effect on the predicted level of infection, with fish held at lower temperatures and smaller fish being more susceptible to piscine francisellosis. Received November 30, 2012; accepted February 22, 2013 相似文献