On the origin of artichoke and cardoon from the <Emphasis Type="Italic">Cynara</Emphasis> gene pool as revealed by rDNA sequence variation

The evolutionary history of artichoke and cultivated cardoon and their relationships to wild allies of the genus Cynara are not fully understood yet. To try resolve the evolutionary patterns leading to the domestication of these two crops, a study of molecular evolution was undertaken. The species C. cardunculus, including artichoke, cultivated and wild cardoon, together with four wild Cynara species were taken into consideration. Internal (ITS) and external (ETS) rDNA transcribed spacers were used as markers of nuclear genome, the psbA-trnH spacer as a marker of chloroplast genome. Sequences were analysed using phylogenetic analysis packages. Molecular data indicate that the whole genus is quite recent and that the domestication of artichoke and cultivated cardoon, crops diverging for reproduction system and use, are independent events which diverge in time and space. As for wild Cynara species, an evolutionary pattern consistent with their present geographical distribution was hypothesized in relation to the climatic changes occurring in the Mediterranean during the last 20 millennia: C. humilis and C. cornigera appeared to have differentiated first, C. syriaca and C. baetica were differentiated in a second period, while C. cardunculus showed to be the most recent and plastic species. The high plasticity of C. cardunculus has not only allowed its nowadays wide distribution, but has also given the potential for domestication. Dedicated to the memory of Richard Neville Lester (1937–2006) who greatly contributed to the understanding of the evolution, domestication, and genetic resources of eggplants as well as to the biosystematics and taxonomy of allied species. IGV Publication N. 78 of the Institute of Plant Genetics, CNR     

Alternatives, traditions, and diversity in agriculture

This review essay examines several recentbooks about agriculture, including two books on thelinks between cultural and biological diversity intraditional agriculture, two books on the US farmcrisis, and a collected volume examining globalaspects of agricultural restructuring andsustainability. Finally, a history of ``alternative'agriculture provides a framework for thinking aboutthe ways the different cases shed light on the complexrelations between tradition and innovation inagriculture. A historical perspective highlights theextent to which ``alternative' is a relative term. Themonocrop, ``factory' mode that dominate US agriculturetoday certainly differs from what has characterizedfarming for most of history and in most of the world.Small-scale, more or less organic, diversified farms,which appear so ``alternative' in the present context,have until lately counted as the norm, here andelsewhere. These books also highlight both the dangersof the currently dominant industrial agriculture andthe potential for genuine alternatives.     

Anterior-posterior guidance of commissural axons by Wnt-frizzled signaling

Commissural neurons in the mammalian dorsal spinal cord send axons ventrally toward the floor plate, where they cross the midline and turn anteriorly toward the brain; a gradient of chemoattractant(s) inside the spinal cord controls this turning. In rodents, several Wnt proteins stimulate the extension of commissural axons after midline crossing (postcrossing). We found that Wnt4 messenger RNA is expressed in a decreasing anterior-to-posterior gradient in the floor plate, and that a directed source of Wnt4 protein attracted postcrossing commissural axons. Commissural axons in mice lacking the Wnt receptor Frizzled3 displayed anterior-posterior guidance defects after midline crossing. Thus, Wnt-Frizzled signaling guides commissural axons along the anterior-posterior axis of the spinal cord.     

Blanching Improves Anthocyanin Absorption from Highbush Blueberry ( Vaccinium corymbosum L.) Purée in Healthy Human Volunteers: A Pilot Study

Blueberries ( Vaccinium corymbosum L.) are rich sources of phenolics and anthocyanins (ACNs). We investigated the absorption of ACNs after consumption of one portion (300 g) of minimally processed blueberry purée (P) obtained from blanched (BL) or unblanched (NB) berries. A repeated-measures, crossover design study was conducted on healthy human volunteers. Blood was drawn between baseline and 24 h after BL-P or NB-P consumption, while urine were collected from the day before the experiment up to 48 h. Total plasma ACN content was not significantly different, while phenolics content was higher in BL-P with respect to NB-P. The maximum ACN absorption in plasma was observed after 1.5 h from the intake of the purées and was significantly higher (p ≤ 0.05) after the intake of BL-P. Both products increased the excretion of hippuric acid in urine. In conclusion, blanching had no significant effect on total ACN content and enhanced their absorption from minimally processed purées.     

Evaluation of the Removal of Pollutants from Petrochemical Wastewater Using A Membrane Bioreactor Treatment Plant

Water, Air, & Soil Pollution - A submerged membrane bioreactor (MBR) was used to treat two types of petrochemical wastewater: olefin process wastewater and total petrochemical wastewater....     

Lignan content of selected foods from Japan

Lignans constitute a group of phytochemicals widely distributed in the human diet. Once ingested, most dietary lignans are metabolized by the gut microflora to enterolactone and enterodiol, also known as enterolignans. Together with isoflavones and coumestrol, enterolignans have been traditionally classified as phytoestrogens, plant-derived compounds that exhibit estrogen-like activity. In addition to a higher intake of vegetables, many studies have suggested that the presence of phytoestrogens in the Japanese diet is one of the factors that might explain the reduced incidence of certain chronic diseases in Japanese populations compared with Western countries. Being a vegetable-based diet, exposure to dietary lignans should be high, but to assess this exposure, a lignan food database is required. Stable isotope-dilution gas chromatography coupled with mass spectrometry was used to quantify six plant lignans in 86 food items commonly consumed in Japan. These data will complement the previous databases and most importantly expand the knowledge of occurrence of lignans in food to Eastern diets.     

Community structure of Lumbricidae in beech woodland of the Bieszczady National Park, Southeast Poland

As ecosystem engineers, earthworms play a key role in the soil environment. However, due to increasing anthropogenic pressure, soil organisms,including earthworms, are being threatened by habitat loss. In this study, we undertook a qualitative and quantitative investigation of earthworms of the family Lumbricidae in four types of Carpathian beech woodland(Fagetum carpaticum), characterized by their understory vegetation(I, F. c. festucetosum drymejae; II, F. c. typicum; III, F. c. lunarietosum; and IV, F. c. allietosum), in the Bieszczady National Park(Eastern Carpathians, Southeast Poland). At each investigated site, soil monoliths(25 cm × 25 cm × 25 cm) were examined by hand sorting. Earthworms were expelled from deep soil layers using a weak formalin solution(0.4%). Depending on the phytocoenosis, 7 species of Lumbricidae were identified at each of the sites I, II, and III and 10 at site IV. Site IV(F. c. allietosum) differed significantly(P 0.05) from the other three sites with respect to earthworm biomass(59.71 ± 39.53 g m~(-2))and the Shannon-Wiener diversity index(0.52 ± 0.12). Although present three decades ago, the deep-burrowing species Octodrilus transpadanus and the litter-dwelling species Dendrobaena octaedra were not found at site IV in the present study. We suspect that these two species may have been displaced by the invasive Lumbricus terrestris, which was not found at the same site in the 1980 s. Such observations warrant further investigation to verify the predicted effects of an invasive earthworm, including the potential effects on soils and other fauna and flora, which have been documented in numerous countries.     

Parental concerns about childhood obesity and the strategies employed to prevent unhealthy weight gain in children

OBJECTIVES: To describe parental concerns about their child's weight, to determine the proportion of parents taking preventive action to avoid obesity in their children and the predictors of taking preventive action, and to describe the strategies adopted by parents. DESIGN: A cross-sectional survey was conducted. Children's heights and weights were measured, and parents completed a questionnaire that included measures of their own weight status, perceptions of their child's weight, concerns about their child's current weight and future weight as an adolescent and adult, and the strategies used to prevent obesity. SETTING: The study was conducted in Melbourne, Australia. SUBJECTS: A total of 291 families of children aged 5-6 years and 919 families of children aged 10-12 years participated.Results: Eighty-nine per cent of parents of overweight 5-6-year-olds and 63% of parents of overweight 10-12-year-olds were unaware their child was overweight. Seventy-one per cent of parents of overweight 5-6-year-olds and 43% of parents of overweight 10-12-year-olds were not concerned about their child's current weight. Although 31% of parents of 5-6-year-olds and 43% of parents of 10-12-year-olds were taking action to prevent unhealthy weight gain in their children, less-educated parents were less likely to do so. CONCLUSIONS: Public health programmes are required to raise parental recognition of childhood overweight and of related risk behaviours, and to provide parents with practical strategies to prevent unhealthy weight gain in their children.     

Root morphology and shoot growth in seedlings of chia (Salvia hispanica L.)

The use of chia (Salvia hispanica L.) seeds as functional food is increasing worldwide and research on plant traits is important as the crop spreads. The seedling stage has been identified as crucial for establishment, survival, and competition with weeds but information on chia at this stage is scanty, especially regarding root traits. In this study, 8 genotypes of chia: 4 populations (Australia, Mexico, Peru, Basilicata) and 4 breeding lines (G3, G8, G17, WS) were evaluated under controlled conditions at two early stages (six leaves, S-6; ten leaves, S-10) with the aim to assess growth characteristics and variability in root morphological traits. The shoot height and dry matter were measured together with the following root traits: dry matter and architectural parameters characterized through image analysis of root scans: length, surface, average diameter, tips, forks and crossings. Plants showed a greater phenotypic variation at S-6 than at S-10 (average CV?=?40% and 22%, respectively). A high variability was recorded for root biomass, root volume, number of forks and crossings (average CV?=?54, 40, 44 and 48%, respectively), while root diameter showed lower variability (average CV?=?10%). Four principal components explained more than 97% of the total variance and root length, surface area, volume and average diameter contributed mainly to the variability. Cluster analysis showed that genotypes were very different for morphological root traits. Australia and G8 had the most developed root system with longer and coarser roots. Our results could be used in a breeding program for new cultivars with a more vigorous seedling.