Evaluation of a modified exogenous creatinine clearance as a suitable renal function test for the small animal practice

A suitable method in the routine veterinary practice for the quantitative determination of the glomerular filtration rate (GFR) in dogs and cats has not been available until to date. Therefore, we modified the known plasma clearance model (=P-CL). The resulting P-CLterminal was assessed concerning its diagnostic value. P-CL of exogenous creatinine (P-CLcrea) and of inulin were determined in dogs (n=12, Beagle, 6 months of age) and cats (n=11, Domestic Short Hair, 14 months of age). The marker substances were administered as a bolus injection. In fasted dogs, P-CLcrea was 84.3 +/- 14.85 ml/min/m2 after a creatinine dose of 2.4 g/m2. An electrolyte infusion during the clearance determination did not alter the resulting values (p>0.05). In fasted cats, P-CLcrea was 54.7 +/- 5.8 ml/min/m2 (creatinine dose 2.0 g/m2). The inulin clearance, determined at the same time, was 104.5 +/- 19.81 ml/min/m2. Feeding the cats just before and during the test increased P-CL of both markers significantly (p<0.05). In order to adapt the clearance method for diagnostic assessment of GFR in the small animal practice, we aimed at minimizing the number of required blood samples (3 instead of 7 or more) and introduced the modified exogenous creatinine clearance (P-CLterminal). These values determined were 108.4 +/- 20.81 ml/min/m2 in fasted dogs and 66.3 +/- 11.81 ml/min/m2 in fasted cats. An electrolyte infusion (dogs) and feeding (cats) had the same effect on P-CLterminal values as described above for P-CL. In conclusion,the modified exogenous creatinine clearance is a suitable renal function test for the early diagnosis of renal disease in dogs and cats presented in small animal practices.     

Intra- and inter-observer reliability of combined segmental measurement techniques for predicting immediate post-deployment intraluminal tracheal stent length in dogs

This study evaluated segmental measurement techniques for predicting immediate post-deployment intraluminal tracheal stent length in dogs with naturally occurring tracheal collapse. Radiographs of 12 client-owned dogs that underwent intraluminal tracheal stent placement were retrospectively reviewed. Tracheal lengths were divided into 1, 2, 3, or 4 equal segments. Stent lengths were predicted using the widest dorsoventral height of each segment, with and without the addition of 10%, and an accompanying foreshortening chart. Techniques were compared for intra- and inter-observer reliability, and post-deployment stent length predictability. There was good to high intra- and inter-observer reliability for all segmental measurements; median intra-class correlation coefficients were 0.98 and 0.92, respectively. Measuring 2 segments without the addition of 10% to the widths was significantly more accurate in predicting immediate post-deployment stent length in terms of percent (P = 0.03) and absolute difference (P = 0.02). Segmental measuring techniques are repeatable amongst observers and may help guide stent selection.     

Evaluation of the oral immunisation of wild boar against classical swine fever in Baden-Württemberg

The oral immunisation of wild boar against classical swine fever (CSF) in Baden-Württemberg is described and evaluated. The bait vaccine based on the CSF virus (CSFV) strain "C" proved to be safe in wild boar of all age classes. The modified immunisation procedure consisting of three double vaccinations per year was very effective. CSFV was not detected beyond the second immunisation campaign. The average rate of seropositive wild boar diagnosed over all immunisation periods was 49.2%. The seroprevalence rate increased significantly during the first year of immunisation and reached its maximum after the third vaccination period with 72% antibody positive animals. The higher percentage of seropositive young boars in this field trial compared to the seroprevalence rates in this age class in other field trials in Germany may be attributed to the new vaccination scheme. Factors that may be responsible for the decreased herd immunity after the fourth or sixth immunisation period are discussed.     

The complete genome sequence of Propionibacterium acnes, a commensal of human skin

Propionibacterium acnes is a major inhabitant of adult human skin, where it resides within sebaceous follicles, usually as a harmless commensal although it has been implicated in acne vulgaris formation. The entire genome sequence of this Gram-positive bacterium encodes 2333 putative genes and revealed numerous gene products involved in degrading host molecules, including sialidases, neuraminidases, endoglycoceramidases, lipases, and pore-forming factors. Surface-associated and other immunogenic factors have been identified, which might be involved in triggering acne inflammation and other P. acnes-associated diseases.     

CCCSNs施入对毛竹叶片光合及生理特性的影响

以3年生水培毛竹实生苗为研究对象,研究不同质量浓度的铜/碳-核/壳结构纳米颗粒(CCCSNs)处理对毛竹抗氧化酶活性、H2O2、MDA含量、光合参数和光系统II(PSII)快速叶绿素荧光诱导动力学参数的影响。结果表明,CCCSNs处理显著提高了毛竹叶片的过氧化氢酶(CAT)活性和H2O2含量,随着CCCSNs处理浓度的增加,叶片的MDA含量逐渐降低,超氧化物歧化酶(SOD)和过氧化物酶(POD)均呈先降低后升高的趋势;CCCSNs处理后毛竹叶片对光的利用率增大,但并未对其光合作用造成显著影响,对毛竹叶片叶绿素荧光动力学参数影响也不显著。表明CCCSNs的施入并未对毛竹叶片的生理和光合作用造成较大影响,不足以对毛竹的生长造成危害,可为后续的CCCSNs对活体毛竹材的防腐效果评价提供参考。     

Making use of the ecosystem services concept in regional planning—trade-offs from reducing water erosion

In this article we demonstrate how to integrate the ecosystem services concept into regional planning using the example of a case study in Saxony, Germany. We analysed how the reduction of water erosion as a regulating service impacts six other ecosystem services. Ecological integrity, provisioning services (provision of food and fibre, provision of biomass), regulating services (soil erosion protection, drought-risk regulation, flood regulation), and the cultural service landscape aesthetics are taken into account. Using a decision support software, we found that the greening of preferential discharge paths can reduce water erosion by 2–7 %. The introduction of hedgerows and the change in the soil management system from tillage to no-till practices revealed a reduction in the total soil loss by 33 and 89 %, respectively. A combination of the three erosion control measures—greening, hedgerows, and no-till management—reduced the soil loss most efficiently by 92 %. We found synergies between the measures for reducing erosion and the provision of ecological integrity, of regulating and cultural ecosystem services. In contrast, the impact on provisioning services was slightly negative. For the land use planning in the case study region we recommend therefore a combination of greening, hedges, and management change. We found that the applied integrated ecosystem services assessment approach, in combination with stakeholder involvement in the scenario development, helped communicating cross-sectoral effects of different management strategies in a comprehensive way and therefore supports regional planning.     

Visualizing tmRNA entry into a stalled ribosome

Bacterial ribosomes stalled on defective messenger RNAs (mRNAs) are rescued by tmRNA, an approximately 300-nucleotide-long molecule that functions as both transfer RNA (tRNA) and mRNA. Translation then switches from the defective message to a short open reading frame on tmRNA that tags the defective nascent peptide chain for degradation. However, the mechanism by which tmRNA can enter and move through the ribosome is unknown. We present a cryo-electron microscopy study at approximately 13 to 15 angstroms of the entry of tmRNA into the ribosome. The structure reveals how tmRNA could move through the ribosome despite its complicated topology and also suggests roles for proteins S1 and SmpB in the function of tmRNA.     

Interference by amino acids during the determination of N ammonium in soil

In the study of terrestrial N cycling, NH₄⁺ concentration and ¹⁵N enrichment are routinely determined by colorimetric continuous flow analysis and microdiffusion methods. Amino acids can interfere in these determinations; consequently the aim of the present study was to evaluate the significance of the interference. Glycine and glutamine are key amino acids in soil and were therefore used as ‘models’. Both glycine and glutamine interfered during continuous flow analysis, whereas interference during microdiffusion was of little importance. The effects of interference can be significant, e.g. estimates of gross mineralisation rate were reduced up to 33%, where we allowed for amino acid interference during determination of NH₄⁺ concentration. The potential influence of amino acid interference emphasises that development of continuous flow analysis to increase NH₄⁺ specificity is needed.