Tuber yield, water and fertilizer productivity in early potato as affected by a combination of irrigation and fertilization

Excessive amounts of irrigation water and fertilizers are often utilized for early potato cultivation in the Mediterranean basin. Given that water is expensive and limited in the semi-arid areas and that fertilizers above a threshold level often prove inefficacious for production purposes but still risk nitrate and phosphorous pollution of groundwater, it is crucial to provide an adequate irrigation and fertilization management. With the aim of achieving an appropriate combination of irrigation water and nutrient application in cultivation management of a potato crop in a Mediterranean environment, a 2-year experiment was conducted in Sicily (South Italy). The combined effects of 3 levels of irrigation (irrigation only at plant emergence, 50% and 100% of the maximum evapotranspiration - ETM) and 3 levels of mineral fertilization (low: 50, 25 and 75 kg ha⁻¹, medium: 100, 50 and 150 kg ha⁻¹ and high: 300, 100 and 450 kg ha⁻¹ of N, P₂O₅ and K₂O) were studied on the tuber yield and yield components, on both water irrigation and fertilizer productivity and on the plant source/sink (canopy/tubers dry weight) ratio. The results show a marked interaction between level of irrigation and level of fertilization on tuber yield, on Irrigation Water Productivity and on fertilizer productivity of the potato crop. We found that the treatments based on 50% ETM and a medium level of fertilization represent a valid compromise in early potato cultivation management. Compared to the high combination levels of irrigation and fertilization, this treatment entails a negligible reduction in tuber yield to save 90 mm ha⁻¹ year⁻¹ of irrigation water and 200, 50 and 300 kg ha⁻¹ year⁻¹ of N, P₂O₅ and K₂O, respectively, with notable economic savings for farmers compared to the spendings that are usually made.     

Predicting Weed Species Occurrence Based on Site Properties and Previous Year's Weed Presence

Probabilities of Setaria spp., Solanum ptycanthum, Helianthus annuus and Abutilon theophrasti occurrence were predicted based on two site property factors and weed species presence in a previous year using logistic regression models. Weed seedling surveys were conducted just prior to post-emergence weed management in two grower-managed fields in the central Platte River Valley of Nebraska, USA at Alda in 1995 and 1996 and at Shelton in 1994, 1995 and 1997. Weed species density data were re-classified as present or absent at each pair of points on the sampling grid, representing quadrat locations either in the pre-emergence herbicide band or between the crop rows. Site property data were collected in March 1995 at Alda and March 1994 at Shelton. Using factor analysis, two independent factors were derived from correlated attributes of relative elevation, percent organic carbon, pH, nitrate, phosphate, and soil texture measured at Alda. Logistic regression models were estimated and parameterized for each weed species at Alda in 1996 based on the two factors (topography and soil type and soil fertility status) and weed species presence in 1995. Performance of these models for each weed species was evaluated using the independent data set from Shelton. Between and on crop row Setaria spp. and Solanum ptycanthum models described these populations at Alda. At Shelton, on row Setaria spp. occurrence and between row Solanum ptycanthum occurrence were adequately predicted. Helianthus annuus or Abutilon theophrasti occurrence was not well predicted even with knowledge of their presence in the previous year, probably as a result of low actual occurrence within a given year. Maps of predicted occurrence have value in directing weed scouting to field locations where the species is most likely to occur.     

Cultivar evaluation and effect of fermentation on antioxidant capacity and in vitro inhibition of α-amylase and α-glucosidase by highbush blueberry (Vaccinium corombosum)

The berry fruits of highbush blueberry (Vaccinium corymbosum) contain bioactive compounds with potential health benefits. The objective was to evaluate blueberries grown in southern Illinois as well as the effect of fermentation, at two different temperatures, on chemical and physical parameters. Fruits from fifteen blueberry cultivars were analyzed. Fruit diameter ranged from 12.8 mm to 18.7 mm, pH from 2.6 to 3.7, reducing sugars from 6.4% to 15.2%, total sugars from 13.9% to 21.6%, total polyphenols from 0.39 to 1.00 mg gallic acid equivalents (GAE)/g blueberry and antioxidant capacity from 5.8 to 10.9 μM Trolox equivalents (TE)/g. In vitro α-amylase and α-glucosidase inhibitory capacity relative to the positive control acarbose, a known anti-diabetic drug, showed a range from 91.8 to 103.3% for α-amylase and from 103.2% to 190.8% for α-glucosidase. Wines prepared from several of these blueberry cultivars were analyzed throughout fermentation and compared at room temperature and cold temperature fermentation for pH (3.5 to 6.3), °Brix (13.6 to 29.7), total polyphenols (375.4 to 657.1 μg GAE/mL wine), and antioxidant capacity (4.5 to 25.1 mM TE). The wines were also tested for their in vitro capacity to inhibit α-amylase and α-glucosidase and maintained similar inhibitory action as the berries. Highbush blueberry cultivars and their fermented beverages are good natural sources of antioxidants and starch-degrading enzyme inhibitors important for type 2 diabetes management.     

Antifeedant activity of some pentacyclic triterpene acids and their fatty acid ester analogues

The 3-O-fatty acid ester derivatives (C(12)-C(18)) of two pentacyclic triterpenic acids, ursolic acid and oleanolic acid, were synthesized under mild esterification conditions in excellent yields (80-85%) and screened for their antifeedant activity, together with the parent acids, against the agricultural pest tobacco caterpillar larvae (Spodoptera litura F) in a no-choice laboratory study. The Urs-12-ene-28-carboxy-3beta-octadecanoate and olean-12-ene-28-carboxy-3beta-hexadecanoate were found to exhibit exceptionally potent antifeedant activities at 50 microg/cm(2) concentration, even after 48 h.     

Analysing the diets of invertebrate predators using terminal restriction fragments

Analysing food webs in agricultural habitats is essential for the development of natural control strategies. Several molecular tools to investigate trophic interactions on a species-specific level have been developed in recent years and their advantages and limitations have been discussed. With this study we introduce another tool, terminal restriction fragment length polymorphism (tRFLP). Generalist predators found in Australian brassica crops, their prey and abundant parasitoids were chosen as a model system to adapt and evaluate the tRFLP approach. Using general primers and selecting six restriction enzymes, we obtained species-specific tRF patterns for 21 of the most abundant arthropods in brassica crops. We detected up to three prey species in the gut contents of laboratory-fed predators. Detection rates differed among predators, ranging between 28 and 100%. The identification of a species-specific tRF pattern was strongly affected by the presence and concentrations of DNA from other species. In a preliminary field study, prey could be identified from 20% of the collected brown lacewings. The advantage of the tRFLP method is the possibility of identifying multiple species at once. This advantage is counterbalanced by methodological limitations, among which the most critical one is the fact that it will be difficult to maintain specificity in highly diverse ecosystems. However, if species diversity is limited and the target sequence and the primers are chosen to increase detection success, the tRFLP method can be used to study trophic interactions in the field.     

Identification of Four New agr Quorum Sensing-Interfering Cyclodepsipeptides from a Marine Photobacterium

During our search for new natural products from the marine environment, we discovered a wide range of cyclic peptides from a marine Photobacterium, closely related to P. halotolerans. The chemical fingerprint of the bacterium showed primarily non-ribosomal peptide synthetase (NRPS)-like compounds, including the known pyrrothine antibiotic holomycin and a wide range of peptides, from diketopiperazines to cyclodepsipeptides of 500–900 Da. Purification of components from the pellet fraction led to the isolation and structure elucidation of four new cyclodepsipeptides, ngercheumicin F, G, H, and I. The ngercheumicins interfered with expression of virulence genes known to be controlled by the agr quorum sensing system of Staphylococcus aureus, although to a lesser extent than the previously described solonamides from the same strain of Photobacterium.     

Antimutagenicity and antiproliferative studies of lipidic extracts from white shrimp (Litopenaeus vannamei)

An organic extract from fresh shrimp (Litopenaeus vannamei) was studied for antimutagenic and antiproliferative properties using Salmonella typhimurium tester strains TA98 and TA100 with metabolic activation (S9) and a cancer cell line (B-cell lymphoma), respectively. Shrimp extract was sequentially fractionated by thin layer chromatography (TLC) and each fraction was tested for antimutagenic and antiproliferative activities. Crude organic extracts obtained from shrimp reduced the number of revertants caused by aflatoxina B(1), showing a dose-response type of relationship. Sequential TLC fractionation of the active extracts produced several antimutagenic and/or antiproliferative fractions. These results suggested that the lipid fraction of the tested species contained compounds with chemoprotective properties that reduce the mutagenicity of AFB(1) and proliferation of a cancer cell line.     

Inheritance of hypoadrenocorticism in bearded collies

OBJECTIVE: To assess heritability and mode of inheritance for hypoadrenocorticism in Bearded Collies. ANIMALS: 635 Bearded Collies. PROCEDURES: Dogs were classified as affected by hypoadrenocorticism or unaffected. Phenotypic and pedigree data were analyzed. Heritability was estimated by use of Bayesian statistical methods. Regressive logistic models for complex segregation analyses were used to characterize mode of inheritance. RESULTS: Hypoadrenocorticism was diagnosed in 60 (9.4%) dogs. Heritability of hypoadrenocorticism was estimated to be 0.76 with both sexes affected with equal probability. Evaluation of the pedigrees did not support a Mendelian autosomal dominant mode of inheritance. Evidence from the complex segregation analysis for a single locus of large effect on hypoadrenocorticism was not convincing. CONCLUSIONS AND CLINICAL RELEVANCE: Hypoadrenocorticism in Bearded Collies is highly heritable. Although a precise genetic mechanism responsible for inheritance of the disorder remains undetermined, breeding decisions must include consideration of the genetic likelihood of passing on this deleterious disorder to offspring of affected dams and sires.