Isolation of a selected microbial consortium from a pesticide-contaminated mix-load site soil capable of degrading the herbicides atrazine and alachlor

Contaminated soil from a 100-year-old mix-load site located in Reading, PA was evaluated for its potential to provide indigenous microorganisms capable of degrading two widely utilized herbicides, atrazine (2-chloro-4-ethylamino-6-isopropylamino-S-triazine; AT) and alachlor (2-chloro-2′,6′-diethyl-N-[methoxymethyl]-acetanilide; AL). Three different locations from the site were chosen for experimentation based on herbicide handling activities. Standard enrichment techniques were used to isolate a selective microbial consortium (SCM) with the desirable degrading capabilities. Three enrichment treatment schemes were evaluated; AT and AL, AL alone, and only AT. Degradative organisms were isolated from only one of the sample locations. Considerable differences in the soil parameters of the three sample locations were found that might have had an effect on the ability of the indigenous microbial populations within the soil to degrade AT and AL. In the initial cultures from this location, degradation occurred in the AT and AL treatment only. Because the AT and AL were the only sources of carbon and nitrogen (N) for the microbes, these results suggest that AL alone was not a sufficient N source. In general, the ability to degrade AL by the SMC was dependent on AT degradation. Alachlor degradation did not begin until approximately 15% of the AT was transformed. Once all of the AT was removed very little further AL degradation occurred. The average half-life (t_1/2) of AT was 7.5 d, while average t_1/2 for AL degradation was 11 d. Individual colonies from the SMC were identified by fatty acids methyl ester (FAME) analysis. Five strains were identified with similarity indexes above 70%. These isolates included the following: Alcaligenes xylosoxydans subsp. denitrificans, Alcaligenes xylosoxydans subsp. xylosoxydans, Pseudomonas putida, Pseudomonas marginalis, and Providencia rustigianii.     

Gaps and biases in the protection of transnational lakes: a global assessment

Context

National borders remain an impediment to efficient preservation of biodiversity and ecosystems. For transboundary water resources, conservation planning becomes more challenging, as competitive interests make these sensitive and productive systems focal points of interstate conflicts.

Objectives

This global study aims to explore the patterns of protection coverage for transnational lakes and their catchments, highlighting gaps and inconsistencies at the protection observed among countries sharing the same lake.

Methods

Identifying 793 transnational lakes globally, we initially investigated protection coverage at their water bodies. Next, we explored protection coverage patterns across each lake’s catchment, in which we also quantified human pressures’ extent and intensity. Socio-economic and political parameters were examined as potential predictors of the observed patterns.

Results

Only half of the world’s transnational lakes are fully or partly covered by existing protected areas. Our analysis demonstrated that in only 37% of the protected transnational lakes, the extent of protection coverage was similar in the neighboring counties sharing the same lake. Protection patterns were not driven by the relative area of the transnational lakes found in the neighboring countries. Moreover, protection cover focuses mainly on lakes’ water surface ignoring the terrestrial surroundings, while more than 75% of the catchments are subjected to intense human pressures.

Conclusions

Providing the first overview of the protection gaps of transnational lakes globally, we highlight a failure of policy responses to cross-border conservation of sensitive freshwater ecosystems. Consequently, such limitations are likely to loom risks for human well‐being and for the initiation of intense conflicts.

     

Aspergillus fumigatus Bronchopneumonia in a Hellenic Shepherd Dog

A 3 yr old intact female Hellenic shepherd dog was referred due to depression, partial anorexia, fever, and a mild productive cough of 2 mo duration. Thoracic radiographs showed increased opacity of all of the left lung lobes. Upon bronchoscopy, a sanguineous, purulent discharge was detected in the tracheal lumen with hyperplastic tissue narrowing the left main stem bronchus. Cultures were positive for bacteria (Bacillus spp. and Clostridium spp.) but negative for fungi. Due to the severity of the lesions, a complete left lung pneumonectomy was performed. Histopathological examination of the excised lung tissues revealed a severe granulomatous bronchopneumonia with numerous alveolar macrophages laden with structures stained positively by periodic acid-Schiff and Grocott stain that had morphology consistent with fungi. PCR and sequencing of internal transcribed spacer regions 1 and 2 from genetic material extracted from paraffin-embedded pulmonary tissue confirmed the presence of Aspergillus fumigatus. Itraconazole was administrated for 5.5 mo and the dog was clinically normal 26 mo after surgery.     

Climatology of Discomfort Index and Air Quality Index in a Large Urban Mediterranean Agglomeration

The paper studies the climatology of the Discomfort Index (DI) and the Common Air Quality Index (CAQI) in a large Mediterranean urban center (Greater Thessaloniki Area), examines the relations between the indices and the climatic conditions in the study area and investigates the linkage between the Urban Heat Island (UHI) effect and the enhanced discomfort and poor air quality in the city center. Discomfort conditions are more enhanced during July and August. Atmospheric conditions when the population suffers discomfort and feels severe stress are found mainly in the summer season hours with total frequency that can be up to about 4% in a month. Throughout the year, air quality conditions are unhealthier in the stations of urban characterization. Very high daily CAQI values are more frequent during the cold period (about 14%) than during the warm period (1?C2%) of the year in the urban stations. Daily DI???24°C could be more associated to increased temperature than relative humidity and is strongly related to high and very high CAQI values. During summer, when daily CAQI is ??76, the feeling of discomfort is the most probable. Consequently, a strong synergy between discomfort and bad air quality exists in summertime. High and very high CAQI values and the discomfort feeling by the majority of the population are related to stagnant atmospheric conditions. During summer season, the poor air quality and the unfavorable comfort conditions for the majority of the population in the city center are associated with the greater frequency of a more intense UHI effect. The above phenomenon is more pronounced during nighttime than daytime.     

Coralligenous assemblages along their geographical distribution: Testing of concepts and implications for management

• 1. The coralligenous habitat was studied at the large Mediterranean scale, by applying a standardized, non‐destructive photo‐sampling protocol, developed in the framework of the CIGESMED project.
• 2. The results provided evidence to support the following statements: (a) the assemblage pattern is not homogeneously distributed across the four Mediterranean ecoregions studied (biotic gradients hypothesis); and (b) the assemblage pattern does not change significantly when the information is aggregated to higher taxonomic levels (taxonomic sufficiency hypothesis).
• 3. Surrogate taxonomic categories higher than species, such as genus and family, can be used to reveal the multivariate pattern of the coralligenous assemblages.
• 4. Although preliminary at the pan‐Mediterranean scale, these outcomes set the scene for future comparisons as more data sets become available but also for comparisons between taxonomic and functional patterns.

     

Improved bioavailability of dietary phenolic acids in whole grain barley and oat groat following fermentation with probiotic Lactobacillus acidophilus , Lactobacillus johnsonii , and Lactobacillus reuteri

The aim of this study was to improve the bioavailability of the dietary phenolic acids in flours from whole grain barley and oat groat following fermentation with lactic acid bacteria (LAB) exhibiting high feruloyl esterase activity (FAE). The highest increase of free phenolic acids was observed after fermentation with three probiotic strains, Lactobacillus johnsonii LA1, Lactobacillus reuteri SD2112, and Lactobacillus acidophilus LA-5, with maximum increases from 2.55 to 69.91 μg g(-1) DM and from 4.13 to 109.42 μg g(-1) DM in whole grain barley and oat groat, respectively. Interestingly, higher amounts of bound phenolic acids were detected after both water treatment and LAB fermentation in whole grain barley, indicating higher bioaccessibility, whereas some decrease was detected in oat groat. To conclude, cereal fermentation with specific probiotic strains can lead to significant increase of free phenolic acids, thereby improving their bioavailability.     

Hepatotoxic Seafood Poisoning (HSP) Due to Microcystins: A Threat from the Ocean?

Cyanobacterial blooms are a major and growing problem for freshwater ecosystems worldwide that increasingly concerns public health, with an average of 60% of blooms known to be toxic. The most studied cyanobacterial toxins belong to a family of cyclic heptapeptide hepatotoxins, called microcystins. The microcystins are stable hydrophilic cyclic heptapeptides with a potential to cause cell damage following cellular uptake via organic anion-transporting proteins (OATP). Their intracellular biologic effects presumably involve inhibition of catalytic subunits of protein phosphatases (PP1 and PP2A) and glutathione depletion. The microcystins produced by cyanobacteria pose a serious problem to human health, if they contaminate drinking water or food. These toxins are collectively responsible for human fatalities, as well as continued and widespread poisoning of wild and domestic animals. Although intoxications of aquatic organisms by microcystins have been widely documented for freshwater ecosystems, such poisonings in marine environments have only occasionally been reported. Moreover, these poisonings have been attributed to freshwater cyanobacterial species invading seas of lower salinity (e.g., the Baltic) or to the discharge of freshwater microcystins into the ocean. However, recent data suggest that microcystins are also being produced in the oceans by a number of cosmopolitan marine species, so that Hepatotoxic Seafood Poisoning (HSP) is increasingly recognized as a major health risk that follows consumption of contaminated seafood.     

Lethal Ozolaimus megatyphlon infection in a green iguana (Iguana iguana rhinolopa).

An imported 2.5-yr-old female green iguana (Iguana iguana rhinolopa) kept in Greece was presented with a history of anorexia and allotriophagy of 1 mo duration. Upon clinical examination, it was cachectic and had severe abdominal distension and fibrous osteodystrophy. Despite treatment, it died a month later. On necropsy, massive accumulations of threadlike nematode parasites were observed in the large intestine, identified as Ozolaimus megatyphlon, a member of the Oxyuridae family of Pharyngodonidae, a usually nonpathogenic intestinal parasite of iguanas. To the authors' knowledge, its presence has not been reported previously in Europe, although one pathogenic infection has been reported previously in Japan. The animal was presumably infected before importation. Although death was attributed to the heavy parasitic overload, the poor diet and terrarium hygiene, and absence of an anthelminthic regime further contributed to the deterioration of the animal's condition. Recognition of this condition, which may be recently introduced or underdiagnosed, may help improve medical and trade standards concerning this species in practice.     

Expressed sequence tags from the phytopathogenic fungus Botrytis cinerea

A large set of genes was identified in the phytopathogenic fungus Botrytis cinerea by using an expressed sequence tag approach. The fungus was grown in axenic culture and a cDNA library was produced. From this library, 6559 ESTs were obtained. The combined sequences represent 3026 unisequences that corresponds to approximately one-quarter of the estimated total number of genes in B. cinerea. Approximately 18% of the ESTs showed significant similarities with genes coding for proteins with known functions,~56% were similar to genes coding for proteins with unknown functions and ~26% were orphans. A substantial B. cinerea gene inventory including putative virulence factors was therefore obtained and is now available at the Génoplante-Info Database interface (http://urgi.infobiogen.fr///Projects/GPiDB/Interface/).