甘肃省肃南县草地治蝗灭鼠工作经验

总结了肃南县１９５５年以来草原治蝗灭鼠为单一人工药物防治发展到生物新技术与新药物相结合的综合治理经验,提出了今后科技力量,开展多渠道,多学科,多部门协作防治的发展方向。     

Mise en évidence de l'expression du virus leucémogène bovin (BLV) dans les lymphocytes de mouton par une technique d'immunofluorescence utilisant des anticorps monoclonauxDetection of bovine leukemia virus (BLV) in sheep lymphocytes by immunofluorescencence test using monoclonal antibodies

An indirect immunofluorescence (IF) test was developed to detect bovine leukemia virus (BLV) antigen expression in infected sheep lymphocytes, using monoclonal antibodies anti BLV-major envelope glycoprotein gp51. Peripheral blood lymphocytes were cultivated for 48 h in presence of phytohemagglutinin (PHA) (50 μg/ml), and then fixed with acetone. The cells were assayed for the IF test. All experimentally infected sheep were positive with this test.     

Equine chronic diarrhea: Traditional Chinese veterinary medicine review

Chronic diarrhea is a common problem in equine practice. Herbal medicine has been used for the treatment of gastrointestinal disease in horses for at least one thousand years. In present-day equine practice in China, the most common approach to treat chronic diarrhea is herbal medicine, or a combination of herbal medicine and acupuncture. Three types of chronic diarrhea in horses will be described in detail in term of symptoms, diagnosis and herbal treatments in this paper. Nineteen herbal formulas for treatment of different types of diarrhea will be introduced. Clinical results of each herbal formula will be evaluated. Acupuncture treatment of this problem will also be included.     

A distinct Mendelian autosomal recessive syndrome involving the association of anotia, palate agenesis, bifid tongue, and polydactyly in the dog.

A presumed genetic syndrome is described in a family of St. Bernards. Four identically affected littermates presented the association of palate agenesis, anotia, incomplete bifid tongue, preaxial hind paw polydactyly, and an extra thoracic vertebra and rib. Pedigree analysis is compatible with an autosomal recessive gene.     

A comparison of the clinical field efficacy and safety of florfenicol and tilmicosin for the treatment of undifferentiated bovine respiratory disease of cattle in western Canada.

We compared the field efficacy of a new antibiotic, florfenicol, with tilmicosin in the treatment of naturally occurring undifferentiated bovine respiratory disease. Beef calves with rectal temperatures greater than 40.5 degrees C and signs compatible with undifferentiated bovine respiratory disease were entered into the trial. Calves were randomly assigned to receive either florfenicol (20 mg/kg bodyweight intramuscularly; 2 injections 48 h apart) or tilmicosin (10 mg/kg bodyweight subcutaneously; 1 injection). Clinical measures of efficacy included mortality, rectal temperature, illness index score, assessment of treatment success or failure, and the number of relapses or reinfections. Performance was assessed based on weight gains from day 0 to day 90. Two hundred and twenty calves entered the trial; 112 received florfenicol and 108 received tilmicosin. Seventeen deaths occurred between day 0 and day 90, but only 10 during the 28-day trial period. Seven calves receiving tilmicosin died, compared with 3 receiving florfenicol (P = 0.20). Of the 220 initial treatments, 45 (20%) were categorized as treatment failures; 27 in the tilmicosin group and 18 in the florfenicol group (P = 0.10). The number of calves experiencing a 2nd relapse was significantly different, with 17 of 30 (57%) calves on tilmicosin compared with 7 of 26 (27%) calves on florfenicol relapsing at least twice (P = 0.02). Average daily gains over 90 days were 1.55 kg/day for florfenicol-treated calves and 1.51 kg/day for tilmicosin-treated calves. No significant adverse reactions were noticed with either drug. Results indicate that florfenicol and tilmicosin are comparable in the treatment of undifferentiated bovine respiratory disease in western Canada.     

低温环境下维生素E水平对初产蛋鸭性激素分泌和抗氧化旨标的影响

本试验旨在研究低温环境(1～7℃)下维生素E水平对初产蛋鸭性激素分泌和抗氧化指标的影响.试验采用玉米-豆粕型基础饲粮(含维生素E 7.9 IU/kg),分别添加17.1(对照组)、57.1、92.1、142.1、192.1、242.1 IU/kg维生素E,组成6种试验饲粮.选取18周龄、平均体重(1.68±0.01) ...     

盐分浓度对星星草种子萌发的影响

星星草（Ｐｕｃｃｉｎｅｌｌｉａｔｅｎｕｉｆｌｏｒａ）种子经不同浓度ＮａＣｌ溶液处理结果表明；与对照相比，低于０．６ｇ／１００ｍｌ的盐浓度对种子萌发有促进作用；介于０．７ｇ／１００ｍｌ—３．６ｇ／１００ｍｌ间，种子的萌发势和累积发芽率显著下降，但尚不影响其萌发潜势，解除盐分限制后，还可正常萌发；高于３．７ｇ／１００ｍｌ后，不仅抑制种子萌发，并对其萌发潜势有显著影响。     

Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene （LHCGR） with superovulation traits in Chinese Holstein heifers

Background: Upon binding luteinizing hormone in the ovary, the luteinizing hormone/choriogonadotropin receptor (LHCGR) is necessary for follicular maturation and ovulation, as well as luteal function. We detected mutations in the LHCGR gene and evaluated their association with superovulation. Methods: Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing, we examined polymorphisms in LHCGR and the genotypes associated with superovulation traits in 127 Chinese Holstein heifers. Results: A G/T polymorphism (ss52050737) in exon 11 was significantly associated with the total number of ova and the number of transferable embryos. Conclusions: LHCGR may be a new predictor for superovulation in Chinese Holstein heifers.     

抗病虫基因新资源:海洋绿藻孔石莼凝集素基因

植物凝集素(Lecin)具有抵抗植物病原菌和农业害虫的活性以及抗病毒活性,在植物保护上具有广阔的应用前景。从海洋绿藻孔石莼中分离到一种凝集素基因序列,为培育抗病虫作物新品种提供了新资源。