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61.
62.
Fornazari F Teixeira CR da Silva RC Leiva M de Almeida SC Langoni H 《Veterinary parasitology》2011,179(1-3):238-241
Considering that Toxoplasma gondii is a parasite of global importance which affects several animal species including humans, the current study aimed to investigate the prevalence of antibodies against T. gondii among 72 white-eared opossums (Didelphis albiventris) from Botucatu Municipality (22°53'S 48°26'W), S?o Paulo State, Brazil. The investigation was carried out from January 2008 to December 2009, when the animals had their blood samples collected and subjected to the modified agglutination test (MAT); 12 specimens had brain samples bioassayed in mice. Seroprevalence was 5.5% (n=4) and bioassays were negative. Older animals had higher prevalence of antibodies against T. gondii. Opossums in closer contact with the urban environment are likely more exposed to T. gondii than animals from the sylvatic environment. 相似文献
63.
Silvia Ribeiro de Souza e Silva Luiz Claúdio de Almeida Barbosa Beatriz King-Diaz Blas Lotina-Hennsen 《Pesticide biochemistry and physiology》2007,87(2):109-114
3,4-Seco-friedelan-3-oic acid was isolated from Maytenus imbricata (Celastraceae). At low concentrations it inhibited non-cyclic electron transport and ATP synthesis in spinach chloroplasts, i.e., it behaved as a Hill reaction inhibitor, and at high concentrations it acts as an uncoupler by enhancing uncoupled electron transport and Mg2+-ATPase activity. 3,4-Seco-friedelan-3-oic acid did not inhibit PSII electron transport from DPC to DCPIPox and photosystem I activity, but it enhanced from TMQH2 to MV, corroborating its action as uncoupler. It inhibits electron flow through PSII from water to sodium silicomolybdate. The whole results indicate that the 3,4-seco-friedelan-3-oic acid target is at the OEC complex enzyme, the donor side of PSII. The fluorescence decay data shows the formation of the K-band, which match this result, acting as inhibitor at the donor side of PSII and it as an uncoupler. 相似文献
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66.
Vieira P Banora MY Castagnone-Sereno P Rosso MN Engler G de Almeida Engler J 《Phytopathology》2012,102(10):990-996
Plant-parasitic nematodes possess a large number of proteins that are secreted in planta, allowing them to be successful parasites of plants. The majority of these proteins are synthesized mainly in the nematode subventral and dorsal glands as well as in other organs. To improve the immunovisualization of these proteins, we adapted a methacrylate embedding method for the localization of proteins inside nematode tissues, and extracellularly when secreted in planta or within plant cells. An important advantage is that the method is applicable for all nematode stages: preparasitic as well as parasitic stages, including large mature females. Herein, the method has been successfully applied for the localization of four nematode secreted proteins, such as Mi-MAP-1, Mi-CBM2-bearing proteins, Mi-PEL3, and Mi-6D4. In addition, we could also localize 14-3-3 proteins, as well as two cytoskeletal proteins, by double-immunolabeling on preparasitic juveniles. Superior preservation of nematode and plant morphology, allowed more accurate protein localization as compared with other methods. Besides excellent epitope preservation, dissolution of methacrylate from tissue sections unmasks target proteins and thereby drastically increases antibody access. 相似文献
67.
Oliveira SC de Almeida LA Carvalho NB Oliveira FS Lacerda TL 《Veterinary immunology and immunopathology》2012,148(1-2):129-135
The innate immune system constitutes an efficient defense mechanism against invading microbial pathogens. Recent studies have revealed the intracellular signaling cascades involved in the TLR-initiated immune response to Brucella spp. infection. However, there is a piece of the puzzle missing that is the role of non-TLR receptors in innate immunity. The involvement of TLR receptors in brucellosis has been investigated by different research groups. It was demonstrated that TLR2 clearly does not play any role in controlling Brucella abortus infection in vivo, whereas TLR9 has been shown to be required for clearance of this bacterium in infected mice. The participation of adaptor molecules, such as MyD88 and TRIF has also been discussed. Recently, we and others have reported the critical role of MyD88- and not TRIF-mediated signaling in dendritic cell maturation and in vivo resistance during B. abortus infection. However, the relationship between specific Brucella molecules and non-TLR receptors and signal transduction pathways needs to be better understood. It is now clear that the interaction between TLRs and recently identified cytosolic innate immune sensors is crucial for mounting effective immune responses. Finally, this review discusses the mechanisms used by Brucella to escape detection by the host innate immune system. 相似文献
68.
G.D. Almeida D.C. Feliz R.P. Heckler D.G.L. Borges M.K.V. Onizuka L.E.R. Tavares F. Paiva F.A. Borges 《Veterinary parasitology》2013,191(1-2):59-65
Ivermectin (IVM) resistance of Cooperia spp. in cattle has become an increasing and global problem. The early detection of anthelmintic resistance (AR) is important to propose strategies to slow down the development of resistance and requires sensitive, reliable, economic high-throughput and practical tests. The purpose of the present study was to apply a larval migration inhibition test (LMIT) for evaluating IVM and MOX efficacy against well-characterized field isolates of Cooperia spp. infecting cattle in Brazil. Eight isolates were used for IVM and seven for MOX. The following EC50 values of IVM were observed for the isolates: susceptible, 1.16 ηmol; Nova Alvorada do Sul I, 4.09 ηmol (RF = 3.52); Campo Grande BNA, 3.57 ηmol (RF = 3.07); Campo Grande TBR, 4.09 ηmol (RF = 3,52); Nova Alvorada do Sul II, 2.50 ηmol (RF = 2.15); Bandeirantes, 11.35 ηmol (RF = 9.78); Campo Grande II, 6.03 ηmol (RF = 5.20); and Porto Mortinho, 8.63 ηmol (RF = 7.44). For MOX, the following EC50 values were observed: susceptible, 0.75 ηmol; Campo Grande BNA, 0.93 ηmol (RF = 1.24); Campo Grande TBR, 0.36 ηmol (RF = 0.48); Nova Alvorada do Sul II, 2.57 ηmol (RF = 3.42); Bandeirantes, 1.43 ηmol (RF = 1.90); Campo Grande II, 1.08 ηmol (RF = 1.44); and Porto Mortinho, 0.49 ηmol (RF = 0.65). The LMIT used in the present study can be a useful tool for in vitro evaluation of IVM, but not of MOX. However, such methodology cannot be used in large-scale studies yet. The isolates of Cooperia spp. showed various degrees of resistance to IVM, though remaining susceptible to MOX. 相似文献
69.
Adelaide Almeida ?ngela Cunha Newton C.M. Gomes Eliana Alves Liliana Costa Maria A.F. Faustino 《Marine drugs》2009,7(3):268-313
Owing to the increasing importance of aquaculture to compensate for the progressive worldwide reduction of natural fish and to the fact that several fish farming plants often suffer from heavy financial losses due to the development of infections caused by microbial pathogens, including multidrug resistant bacteria, more environmentally-friendly strategies to control fish infections are urgently needed to make the aquaculture industry more sustainable. The aim of this review is to briefly present the typical fish farming diseases and their threats and discuss the present state of chemotherapy to inactivate microorganisms in fish farming plants as well as to examine the new environmentally friendly approaches to control fish infection namely phage therapy and photodynamic antimicrobial therapy. 相似文献
70.
Reto M Figueira ME Filipe HM Almeida CM 《Plant foods for human nutrition (Dordrecht, Netherlands)》2007,62(4):139-144
To evaluate the potential benefits and risks associated with tea consumption it is important to identify the constituents
of this beverage. Levels of some minerals, caffeine and catechins in green tea samples commercialized in Portugal were evaluated.
Potassium is the metal present in larger amount (92–151 mg/l). The content of sodium, calcium, fluoride, aluminium, manganese
and iron were 35–69, 1.9–3.5, 0.80–2.0, 1.0–2.2, 0.52–1.9, 0.020–0.128 mg/l, respectively. Chromium and selenium were not
detected. The resulting data showed considerable variability in catechins content. The levels of epigallocatechin gallate
(EGCG) ranged from 117 to 442 mg/l, epicatechin 3-gallate (EGC) from 203 to 471 mg/l, epigallocatechin (ECG) from 16.9 to
150 mg/l, epicatechin (EC) from 25 to 81 mg/l and catechin (C) from 9.03 to 115 mg/l. Caffeine contents in the green tea infusions
studied were between 141–338 mg/l. Green tea infusions provide significant amounts of catechins and could be an important
source of some minerals. 相似文献