Clinically significant,nontraumatic, degenerative joint disease of the temporomandibular joints in a horse

Horses with degenerative joint disease (DJD) of the temporomandibular joint (TMJ) have been reported infrequently, with the majority of cases describing the disease as a consequence of an earlier traumatic event. A case of clinically significant TMJ‐DJD due to a nontraumatic event has not been published. This retrospective case report describes a case of bilateral, DJD of the TMJ. Case management, subsequent review and interpretation of the clinical records, tangential radiographic views of each TMJ, computed tomography and magnetic resonance imaging of the head, and post mortem histological examination of the TMJs were performed. The horse exhibited both quidding and a ‘clicking’ sound during mastication; episodes of intermittent colic were also prevalent in the horse's history. Computed tomography illustrated bilateral mineralisation of the rostral aspect of both intra‐articular discs. Treatment, by intra‐articular injection of corticosteroid, resulted in temporary resolution of both the quidding and the ‘clicking’ sound, as well as the recurrent episodes of colic. Repeated treatment over time was required. Ultimately the horse was subjected to euthanasia for reasons other than disease of the TMJ. The development of TMJ‐DJD may not be confined to traumatic events. Age‐related degeneration of this joint may occur and manifest through quidding and abnormal sounds noted during mastication.     

Effect of microbial‐based inoculants on nutrient concentrations and early root morphology of corn (Zea mays)

Microbial‐based inoculants have been reported to stimulate plant growth and nutrient uptake. However, their effect may vary depending on the growth stage when evaluated or fertilizer applied. Thus, the objective of this study was to test the hypothesis that microbial‐based inoculants known to promote root growth and nutrient uptake will promote plant growth, enhance early root development, and increase nutrient concentrations of corn (Zea mays L.). Plants were evaluated at four different growth stages and in the presence of three different nitrogen (N) fertilizers. The microbial‐based treatments evaluated were: SoilBuilder™ (SB), a filtered metabolite extract of SoilBuilder™ (SBF), a mixture of four strains of plant growth‐promoting Bacillus spp (BM), and a water‐inoculated control. The experiment also included four fertilizer treatments: urea (U), urea‐ammonium nitrate (UAN), calcium‐ammonium nitrate (CAN), and an unfertilized control. Corn plants were evaluated at growth stages V2, V4, V6, and VT. Plant growth parameters for biomass, height, and SPAD readings were enhanced by the three microbial‐based treatments. A greater effect of microbial‐based treatments was observed when plants were evaluated at V6 and VT stages. Parameters of early root development such as total root length (TRL), root surface area (RSA), and length of fine roots were enhanced when microbial‐based treatments were applied. Concentrations of N, P, and K were also increased by microbial‐based treatments compared to the non‐inoculated control. Increases in plant N concentration due to microbial‐based treatments were on average 72% for CAN, 61% for UAN, 72% for urea, and 54% for the unfertilized control. Phosphorus concentration was increased most (138%) when BM was applied with CAN. In the same way, when CAN was present, K concentration was increased by 95% with BM and 65% when SB and SBF were applied. Overall, the results demonstrate that microbial‐based inoculants evaluated in this study can positively impact corn growth and nutrient concentration, especially during the late vegetative stages. Furthermore, the results indicate that the enhancement of nutrient concentrations (N, P, and K) in this case was related to the capacity of microbial‐based treatments to impact root morphology at early stages of corn growth.     

Variation of mineral nutrient contents of modern and heirloom cultivars of cabbage in different regimes of soil fertility

An improvement in the mineral nutrient contents of fruits and vegetables is needed to offset reported declines in concentrations of these elements in fruits and vegetables. The declines have been associated with the high productivity of modern cultivars and to depleted soil fertility. This research addressed differences in mineral nutrient concentrations between modern F1 hybrids and heirloom cultivars of cabbage (Brassica oleraceae var. capitata L.)and among fertilization practices with conventional chemical or organic fertilizers and compost. Crop production was greater with the chemical or organic fertilizers than with the compost. Mineral nutrient composition did not vary between modern or heirloom cultivars or among fertilization regimes but varied among cultivars, suggesting that cultivar selection could lead to production of nutrient-rich cabbage. Neither mass of heads nor days to maturation of crops affected nutrient composition.     

Impact of vitronectin concentration and surface properties on the stable propagation of human embryonic stem cells

The standard method for culturing human embryonic stem cells (hESC) uses supporting feeder layers of cells or an undefined substrate, Matrigel(?), which is a basement membrane extracted from murine sarcoma. For stem cell therapeutic applications, a superior alternative would be a defined, artificial surface that is based on immobilized human plasma vitronectin (VN), which is an adhesion-mediating protein. Therefore, VN adsorbed to diverse polymer surfaces was explored for the continuous propagation of hESC. Cells propagated on VN-coated tissue culture polystyrene (TCPS) are karyotypically normal after >10 passages of continuous culture, and are able to differentiate into embryoid bodies containing all three germ layers. Expansion rates and pluripotent marker expression verified that a minimal VN surface density threshold is required on TCPS. Further exploration of adsorbed VN was conducted on polymer substrates with different properties, ranging from hydrophilic to hydrophobic and including cationic and anionic polyelectrolyte coatings. Despite differing surface properties, these substrates adsorbed VN above the required surface density threshold and were capable of supporting hESC expansion for >10 passages. Correlating wettability of the VN-coated surfaces with the response of cultured hESC, higher cell expansion rates and OCT-4 expression levels were found for VN-coated TCPS, which exhibits a water contact angle close to 65°. Importantly, this simple, defined surface matches the performance of the benchmark Matrigel, which is a hydrogel with highly complex composition.     

Effect of polymyxin B and environmental conditions on isolation of Brucella species and the vaccine strain RB51

Brucella are resistant to polymyxin B (PB), but their relative susceptibility to PB and its derivative, colistin (COL) has not been rigorously or systematically studied. Comparative susceptibility of Brucella reference strains, vaccine strain RB51, and Brucella isolates from marine mammals to these two cationic peptides were determined by Etest. Vast differences among Brucella species were found in susceptibility to both PB and COL. Brucella demonstrated similar pattern of relative susceptibility to PB as that of COL, but they were less susceptible to COL. Both B. melitensis and B. suis were the least susceptible to polymyxins and rough strains were more susceptible to both PB and COL than the smooth except for the BvrR mutant. Strains were generally less susceptible to PB when cultured in CO(2) rather than ambient air; some became more susceptible in acidified medium. Results show that environment cultural conditions must be considered when selecting for CO(2)-independent strains of Brucella especially the vaccine strain RB51 on selective media containing PB. Our observations extend basic knowledge of the differential resistance of Brucella to polymyxins.     

Diviner Lunar Radiometer observations of cold traps in the Moon's south polar region

Diviner Lunar Radiometer Experiment surface-temperature maps reveal the existence of widespread surface and near-surface cryogenic regions that extend beyond the boundaries of persistent shadow. The Lunar Crater Observation and Sensing Satellite (LCROSS) struck one of the coldest of these regions, where subsurface temperatures are estimated to be 38 kelvin. Large areas of the lunar polar regions are currently cold enough to cold-trap water ice as well as a range of both more volatile and less volatile species. The diverse mixture of water and high-volatility compounds detected in the LCROSS ejecta plume is strong evidence for the impact delivery and cold-trapping of volatiles derived from primitive outer solar system bodies.     

Control of Zebra Disease of Agave Hybrids by Breeding for Resistance to Phytophthora spp.

Abstract

A zebra disease resistance factor has been incorporated into the high-yielding Agave hybrid no. 11648 by crossing this hybrid with A. lespinassei. The progeny of three crosses have been screened for resistance to Phytophthora nicotianae, the chief pathogen of zebra disease and results show that 56.7% of the plants have sufficient resistance to be of economic value. The two screening techniques used are described, and the nature of the resistance factor discussed.