全文获取类型
收费全文 | 1683篇 |
免费 | 110篇 |
专业分类
林业 | 99篇 |
农学 | 29篇 |
基础科学 | 11篇 |
255篇 | |
综合类 | 357篇 |
农作物 | 63篇 |
水产渔业 | 105篇 |
畜牧兽医 | 773篇 |
园艺 | 36篇 |
植物保护 | 65篇 |
出版年
2023年 | 16篇 |
2022年 | 26篇 |
2021年 | 42篇 |
2020年 | 49篇 |
2019年 | 51篇 |
2018年 | 37篇 |
2017年 | 35篇 |
2016年 | 47篇 |
2015年 | 24篇 |
2014年 | 43篇 |
2013年 | 65篇 |
2012年 | 103篇 |
2011年 | 121篇 |
2010年 | 68篇 |
2009年 | 50篇 |
2008年 | 115篇 |
2007年 | 90篇 |
2006年 | 95篇 |
2005年 | 72篇 |
2004年 | 56篇 |
2003年 | 72篇 |
2002年 | 70篇 |
2001年 | 17篇 |
2000年 | 13篇 |
1999年 | 14篇 |
1996年 | 13篇 |
1994年 | 11篇 |
1993年 | 8篇 |
1992年 | 15篇 |
1991年 | 13篇 |
1990年 | 10篇 |
1989年 | 10篇 |
1988年 | 8篇 |
1987年 | 10篇 |
1986年 | 18篇 |
1985年 | 15篇 |
1984年 | 10篇 |
1983年 | 9篇 |
1979年 | 11篇 |
1978年 | 12篇 |
1977年 | 10篇 |
1976年 | 9篇 |
1975年 | 10篇 |
1974年 | 10篇 |
1973年 | 12篇 |
1972年 | 7篇 |
1971年 | 13篇 |
1969年 | 12篇 |
1968年 | 7篇 |
1967年 | 13篇 |
排序方式: 共有1793条查询结果,搜索用时 15 毫秒
981.
Roberta B. Ferreira Jair C. Moraes Alexander M. Auad Marcy G. Fonseca 《Journal of pest science》2013,86(2):161-166
This study evaluated the impact of different CO2 levels on the biological characteristics of Mahanarva spectabilis (Distant) and on the performance of forage grasses. The signal grasses Brachiaria decumbens Stapf (susceptible) and Brachiaria brizantha (A. Rich.) (resistant), the elephant grass Pennisetum purpureum (Schum.), including the Roxo de Botucatu cultivar (susceptible) and the Pioneiro cultivar (resistant) and the insects were kept in climate-controlled chambers with constant low (250 ppm) CO2 levels, constant high (500 ppm) CO2 levels, or fluctuating CO2 levels (mean, 368 ppm). Among these three CO2 treatments, no significant differences were found in the nymphal survival of M. spectabilis when the nymphs were fed on two signal grass species. On the other hand, under a constant low CO2 level (250 ppm), nymphal survival rates were significantly lower when the insects were kept in Roxo de Botucatu and Pioneiro cultivars. The mean adult longevity values for M. spectabilis were not significantly different among the three levels of CO2 evaluated. We observed that increased CO2 levels improved the performance of M. spectabilis and elephant grass cultivars in accumulating dry mass, which was not evident in signal grass. We further conclude that the levels of susceptibility and resistance of the forages tested will be maintained in future scenarios under which atmospheric CO2 levels are expected to increase. 相似文献
982.
Alexander Kobler Yves Humblet Guy Knaepkens Brecht Engelen Marcel Eens 《Ecology of Freshwater Fish》2012,21(3):453-460
Abstract – Passive integrated transponder tags have been successfully applied in Cottus spp. and have enabled researchers to gather more information about the movement patterns of individual fish in the wild. In two succeeding years during springtime, a portable antenna was used to determine diel movements of bullhead (Cottus perifretum). In 2007, bullhead (N = 26) moved significantly farther distances at night (mean, 0.42 m·h?1) and dawn (mean, 0.35 m·h?1) than during daytime (mean, 0.11 m·h?1; mixed model, P < 0.001, respectively; P = 0.001), which may be due to foraging activities for Gammarus spp. Irrespective of diel period, smaller fish covered significantly longer distances (P = 0.001). In 2008, similar diel movement patterns were observed, but the differentiation between daytime (mean, 0.12 m·h?1) and night periods (mean, 0.18 m·h?1) was not significant (mixed model, P = 0.087; N = 49 bullhead). It is discussed that longer tracking intervals used in 2008 (three times per 24‐h instead of every two hours) were not suitable to detect the sheer magnitude of distances covered during a diel period. It is shown that this may be due to ‘site fidelity’ of some individuals: after swimming several metres at night, they returned to the exact location they previously occupied during daytime. In 2008, sex and body size were not related to diel movement. The present study is the first to present a quantitative differentiation between diel distances covered in a Cottus spp. 相似文献
983.
Dilier Olivera Viciedo Renato de Mello Prado Carlos A. Martinez Eduardo Habermann Roberto Botelho Ferraz Branco Marisa de Cssia Piccolo Alexander Calero Hurtado Kolima Pea Calzada Luis F. Lata Tenesaca 《Journal of Agronomy and Crop Science》2021,207(1):128-138
Temperature and other abiotic factors, such water and nutrient availability, play an important role for plants in response to the changing environments. At this regard, both warming and drought might affect the nutrient use efficiency (NUE) and growth of Megathyrsus maximus a C4 forage grass of high interest for cattle feeding. However, the nutrient requirements of this species under climate change are unknown. Therefore, we aimed to evaluate the individual and combined effects of two levels of temperature: ambient and elevated temperature (2°C above ambient temperature), and two levels of soil water availability: irrigated plants and non‐irrigated plants on accumulation of leaf nutrients, NUE and biomass production of M. maximus. Temperature control was performed by a temperature free‐air‐controlled enhancement (T‐FACE) system under field conditions. In general, we observed that warming under well‐irrigated conditions increased the leaf accumulation of most nutrients, improving the NUE of N, P, K, Ca, Mg, Cu, Mn and Zn. Plant growth was also enhanced under warming effects, with higher leaf dry mass accumulation and root development. Meanwhile, drought decreased NUE of K, Ca, B and leaf dry biomass, while root growth was stimulated. The combined effects of warming and drought on nutrient accumulation, NUE and plant growth tended to be greater than the individual effects expected from a single factor; thus, warming mitigated the negative impacts of individual drought. In summary, our findings suggest that warming and drought, both as individual and combined factors, will change the nutrient requirements of M. maximus in tropical ecosystems. 相似文献
984.
A significant drop in the prevalence of feline leukaemia virus (FeLV) antigenaemic cats and antigen-associated lymphomas has been observed after the introduction of FeLV vaccination and antigen-testing with removal of persistently antigenaemic cats. However, recent reports have indicated that regressively infected cats may contain FeLV provirus DNA and that lymphoma development may be associated with the presence of provirus alone. In the present study, we investigated the presence of FeLV antigen and provirus DNA in 50 lymphomas by immunohistochemistry and semi-nested polymerase chain reaction, respectively. Interestingly, almost 80% of T-cell lymphomas and 60% of B-cell lymphomas contained provirus DNA while only 21% of T-cell lymphomas and 11% of B-cell lymphomas expressed FeLV antigen. In conclusion, our results support previous hypotheses that vaccination and removal of persistently antigenaemic cats have led to a drop in FeLV antigen-expressing lymphomas. However, FeLV provirus DNA is still present in a high percentage of feline lymphomas. 相似文献
985.
986.
Koutinas AF Carlotti DN Koutinas C Papadogiannakis EI Spanakos GK Saridomichelakis MN 《Veterinary dermatology》2010,21(6):572-577
Histological lesions and the presence of Leishmania spp. amastigotes in claw tissues were investigated in 40 dogs with leishmaniosis, with (16/40--group A) or without (24/40--group B) generalized onychogryphosis. Following euthanasia, the entire third phalanx with intact claw was amputated, formalin fixed, decalcified in a formic acid solution, embedded in paraffin, sectioned longitudinally and stained with haematoxylin and eosin, and acid orcein-Giemsa. Nested polymerase chain reaction (PCR) was used for the detection of Leishmania amastigotes. Lichenoid mononuclear infiltration (all dogs in group A, 21 of 24 dogs in group B), basal keratinocyte vacuolation (nine of 16 dogs in group A, 15 of 24 dogs in group B) and dermoepidermal clefting (13 of 16 dogs in group A, 18 of 24 dogs in group B) were the most prominent histopathological findings. There was no difference in the frequency and severity of these lesions between the two groups. Leishmania amastigotes could not be visualized in the dermis of any of the H&E sections, but their presence was demonstrated by nested PCR in three of 16 dogs in group A and two of 24 dogs in group B. However, the frequency of positive nested PCRs was not significantly different between the two groups. In conclusion, claw histopathology in symptomatic dogs with leishmaniosis, either with or without onychogryphosis is mainly characterized by mononuclear lichenoid dermatitis with or without interface dermatitis and dermoepidermal clefting, and is not accompanied by substantial local parasitism. 相似文献
987.
The evolution and future of Earth's nitrogen cycle 总被引:4,自引:0,他引:4
Atmospheric reactions and slow geological processes controlled Earth's earliest nitrogen cycle, and by ~2.7 billion years ago, a linked suite of microbial processes evolved to form the modern nitrogen cycle with robust natural feedbacks and controls. Over the past century, however, the development of new agricultural practices to satisfy a growing global demand for food has drastically disrupted the nitrogen cycle. This has led to extensive eutrophication of fresh waters and coastal zones as well as increased inventories of the potent greenhouse gas nitrous oxide (N(2)O). Microbial processes will ultimately restore balance to the nitrogen cycle, but the damage done by humans to the nitrogen economy of the planet will persist for decades, possibly centuries, if active intervention and careful management strategies are not initiated. 相似文献
988.
Mitrovich QM Tuch BB De La Vega FM Guthrie C Johnson AD 《Science (New York, N.Y.)》2010,330(6005):838-841
The evolutionary forces responsible for intron loss are unresolved. Whereas research has focused on protein-coding genes, here we analyze noncoding small nucleolar RNA (snoRNA) genes in which introns, rather than exons, are typically the functional elements. Within the yeast lineage exemplified by the human pathogen Candida albicans, we find--through deep RNA sequencing and genome-wide annotation of splice junctions--extreme compaction and loss of associated exons, but retention of snoRNAs within introns. In the Saccharomyces yeast lineage, however, we find it is the introns that have been lost through widespread degeneration of splicing signals. This intron loss, perhaps facilitated by innovations in snoRNA processing, is distinct from that observed in protein-coding genes with respect to both mechanism and evolutionary timing. 相似文献
989.
Marchitto TM Muscheler R Ortiz JD Carriquiry JD van Geen A 《Science (New York, N.Y.)》2010,330(6009):1378-1381
We present a high-resolution magnesium/calcium proxy record of Holocene sea surface temperature (SST) from off the west coast of Baja California Sur, Mexico, a region where interannual SST variability is dominated today by the influence of the El Ni?o-Southern Oscillation (ENSO). Temperatures were lowest during the early to middle Holocene, consistent with documented eastern equatorial Pacific cooling and numerical model simulations of orbital forcing into a La Ni?a-like state at that time. The early Holocene SSTs were also characterized by millennial-scale fluctuations that correlate with cosmogenic nuclide proxies of solar variability, with inferred solar minima corresponding to El Ni?o-like (warm) conditions, in apparent agreement with the theoretical "ocean dynamical thermostat" response of ENSO to exogenous radiative forcing. 相似文献
990.
Rubtsov YP Niec RE Josefowicz S Li L Darce J Mathis D Benoist C Rudensky AY 《Science (New York, N.Y.)》2010,329(5999):1667-1671
Tissue maintenance and homeostasis can be achieved through the replacement of dying cells by differentiating precursors or self-renewal of terminally differentiated cells or relies heavily on cellular longevity in poorly regenerating tissues. Regulatory T cells (T(reg) cells) represent an actively dividing cell population with critical function in suppression of lethal immune-mediated inflammation. The plasticity of T(reg) cells has been actively debated because it could factor importantly in protective immunity or autoimmunity. By using inducible labeling and tracking of T(reg) cell fate in vivo, or transfers of highly purified T(reg) cells, we have demonstrated notable stability of this cell population under physiologic and inflammatory conditions. Our results suggest that self-renewal of mature T(reg) cells serves as a major mechanism of maintenance of the T(reg) cell lineage in adult mice. 相似文献