全文获取类型
收费全文 | 457篇 |
免费 | 23篇 |
专业分类
林业 | 29篇 |
农学 | 12篇 |
117篇 | |
综合类 | 53篇 |
农作物 | 27篇 |
水产渔业 | 54篇 |
畜牧兽医 | 148篇 |
园艺 | 13篇 |
植物保护 | 27篇 |
出版年
2023年 | 3篇 |
2022年 | 3篇 |
2021年 | 21篇 |
2020年 | 21篇 |
2019年 | 18篇 |
2018年 | 12篇 |
2017年 | 19篇 |
2016年 | 28篇 |
2015年 | 20篇 |
2014年 | 16篇 |
2013年 | 14篇 |
2012年 | 38篇 |
2011年 | 48篇 |
2010年 | 18篇 |
2009年 | 25篇 |
2008年 | 26篇 |
2007年 | 30篇 |
2006年 | 17篇 |
2005年 | 19篇 |
2004年 | 26篇 |
2003年 | 11篇 |
2002年 | 17篇 |
2001年 | 1篇 |
2000年 | 4篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1993年 | 3篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
排序方式: 共有480条查询结果,搜索用时 0 毫秒
31.
Cocchi M Franchini G Manzini D Manfredini M Marchetti A Ulrici A 《Journal of agricultural and food chemistry》2004,52(13):4047-4056
A comparison of different digestion procedures has been carried out for the analysis of metal concentration in samples of vinegars and Aceto Balsamico Tradizionale of Modena (ABTM) coming from an unique barrel set. In particular, classical wet, dry ashing, and closed vessel microwave digestion procedure have been utilized and compared for each investigated species. In a few cases, direct metal determination on ABTM (without treatment procedure) is proposed as possible alternative to sample manipulation. Flame atomic absorption spectrometry was used for the quantification of iron and zinc, while graphite furnace atomic absorption spectrometry was used for all the other elements (i.e., chromium, manganese, cobalt, nickel, copper, cadmium, and lead). The comparison among the different sample treatments was carried out by the use of statistical and chemometric tools. In particular, principal component analysis and ANOVA approaches were used to discriminate between the diverse analytical methods. Furthermore, for all the dissolving techniques, the analytical metal recovery was always evaluated by the application of the recovery function on the same sample matrix. In general, the recoveries were fairly good, ranging from 90 to 103%, except for Cd and Pb with dry ashing, which showed recovery values close to 55% and 67%, respectively. As regards the metals concentration of the investigated samples, the experimental data reveal for some species the presence of concentration slightly over the legal limit fixed for wine and wine vinegar. 相似文献
32.
Nino Tavares Amazonas Luiz Antonio MartinelliMarisa de Cássia Piccolo Ricardo Ribeiro Rodrigues 《Forest Ecology and Management》2011,262(8):1551-1557
We considered whether ecological restoration using high diversity of native tree species serves to restore nitrogen dynamics in the Brazilian Atlantic Forest. We measured δ15N and N content in green foliage and soil; vegetation N:P ratio; and soil N mineralization in a preserved natural forest and restored forests of ages 21 and 52 years. Green foliage δ15N values, N content, N:P ratio, inorganic N and net mineralization and nitrification rates were all higher, the older the forest. Our findings indicate that the recuperation of N cycling has not been achieved yet in the restored forests even after 52 years, but show that they are following a trajectory of development that is characterized by their N cycling intensity becoming similar to a natural mature forest of the same original forest formation. This study demonstrated that some young restored forests are more limited by N compared to mature natural forests. We document that the recuperation of N cycling in tropical forests can be achieved through ecological restoration actions. 相似文献
33.
Carbon limitations to nitrous oxide emissions in a humid tropical forest of the Brazilian Amazon 总被引:2,自引:0,他引:2
D.?C.?Garcia-MontielEmail author J.?M.?Melillo P.?A.?Steudler C.?C.?Cerri M.?C.?Piccolo 《Biology and Fertility of Soils》2003,38(5):267-272
The availability of labile organic C for microbial metabolic processes could be an important factor regulating N2O emissions from tropical soils. We explored the effects of labile C on the emissions of N2O from a forest soil in the State of Rondônia in the southwestern quadrant of the Brazilian Amazon. We measured emissions of N2O from a forest soil after amendments with solutions containing glucose, water only or NO3–. Addition of glucose to the forest soil resulted in very large increases in N2O emissions whereas the water only and NO3– additions did not. These results suggest a strong C limitation on N2O production in this forest soil in the southwestern Amazon. 相似文献
34.
Quantitative aspects of solid-state C-NMR spectra of humic substances from soils of volcanic systems
Cross-Polarisation Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance spectroscopy (CPMAS 13C-NMR) represents one of the most powerful tools to investigate soil organic matter (SOM) mainly because of its inherent capacity to provide a semi-quantitative evaluation of carbon distribution. A critical parameter during acquisition of CPMAS 13C-NMR spectra is the contact time required to obtain the cross-polarisation between proton and carbon nuclei. The procedure to evaluate the best contact time for the acquisition of a quantitative CPMAS 13C-NMR spectrum is to perform Variable Contact Time (VCT) experiments. In this work the structural features of a number of purified humic substances from Italian and Costarican volcanic soils were investigated by CPMAS 13C-NMR spectroscopy after having performed preliminary VCT experiments. The VCT experiments showed that the average contact times vary according to the origin and chemical structure of the humic material. The optimal contact times (OCT) for nine humic samples were between 250 and 800 μs These values were different from the time of 1000 μs that is commonly applied as the best average contact time for humic materials. Moreover, by comparing the NMR data to those obtained by elemental analysis (C/H ratio), it appeared that the efficiency of the cross-polarisation between protons and carbons, and hence the contact time, is affected not only by the number of protons, but also by their distribution over the molecules. The evaluation of errors in quantitative estimation of the different carbons revealed that the use of OCT generally reduced by half the loss of signals occurring when the average contact time of 1000 μs is used in CPMAS 13C-NMR spectra of humic substances. 相似文献
35.
Frank-Thorsten Krell Arthur Y.C. Chung Emma DeBoise Paul Eggleton Alessandro Giusti Kelly Inward Sylvia Krell-Westerwalbesloh 《Pedobiologia》2005,49(2):175-186
Winkler extractors, a simple device presumed to extract macro-invertebrates efficiently from soil and litter samples, is being used increasingly in ecological surveys and functional studies of soil macro-invertebrate communities. In this study the extraction efficiency and taxonomic bias of the Winkler extraction are evaluated for extraction periods of 3 h up to 7 weeks, calibrated by hand-sorting after 7 weeks. The method extracts most macro-invertebrates completely or to a proportion of over 90% except Isopoda, Diplopoda and Mollusca. However, for an exhaustive result, a long extraction period of several weeks is necessary. For the most speciose group (adult beetles) and for the commonly most abundant group (ants), a short extraction of 3 days was sufficient to get 70% of the individuals and nearly all species. Three days was also sufficient to recover the rank abundance order of beetle families, while for ‘higher taxa’ and for Chilopoda species, 4 and 3 weeks were necessary, respectively. Optimum extraction times for the abundant macro-invertebrate groups and possible adjustment factors for the soil macro-invertebrates of temperate woodlands are proposed to compensate the taxonomic bias caused by short extraction periods. However, for recording an accurate snapshot of the soil and litter fauna at a particular time, shorter extraction periods are advisable because of the short life cycle of many soil invertebrates causing emergence of later stages or a second generation during longer extraction periods. The problem of contamination of samples is also discussed. 相似文献
36.
Cordenonsi M Montagner M Adorno M Zacchigna L Martello G Mamidi A Soligo S Dupont S Piccolo S 《Science (New York, N.Y.)》2007,315(5813):840-843
During development and tissue homeostasis, cells must integrate different signals. We investigated how cell behavior is controlled by the combined activity of transforming growth factor-beta (TGF-beta) and receptor tyrosine kinase (RTK) signaling, whose integration mechanism is unknown. We find that RTK/Ras/MAPK (mitogen-activated protein kinase) activity induces p53 N-terminal phosphorylation, enabling the interaction of p53 with the TGF-beta-activated Smads. This mechanism confines mesoderm specification in Xenopus embryos and promotes TGF-beta cytostasis in human cells. These data indicate a mechanism to allow extracellular cues to specify the TGF-beta gene-expression program. 相似文献
37.
Much current experimental research on transport in molecular junctions focuses on finite voltages, where substantial polarization-induced nonlinearities may result in technologically relevant device-type responses. Because molecules have strong polarization responses to changing charge state or external field, molecules isolated between electrodes can show strongly nonlinear current-voltage responses. For small applied voltages (up to approximately 0.3 volt), weak interaction between transporting electrons and molecular vibrations provides the basis for inelastic electron tunneling spectroscopy. At higher voltages and for certain time scale regimes, strong coupling effects occur, including Coulomb blockade, negative differential resistance, dynamical switching and switching noise, current hysteresis, heating, and chemical reactions. We discuss a general picture for such phenomena that arise from charging, strong correlation, and polarization (electronic and vibrational) effects in the molecule and at the interface. 相似文献
38.
Aiuti A Slavin S Aker M Ficara F Deola S Mortellaro A Morecki S Andolfi G Tabucchi A Carlucci F Marinello E Cattaneo F Vai S Servida P Miniero R Roncarolo MG Bordignon C 《Science (New York, N.Y.)》2002,296(5577):2410-2413
Hematopoietic stem cell (HSC) gene therapy for adenosine deaminase (ADA)-deficient severe combined immunodeficiency (SCID) has shown limited clinical efficacy because of the small proportion of engrafted genetically corrected HSCs. We describe an improved protocol for gene transfer into HSCs associated with nonmyeloablative conditioning. This protocol was used in two patients for whom enzyme replacement therapy was not available, which allowed the effect of gene therapy alone to be evaluated. Sustained engraftment of engineered HSCs with differentiation into multiple lineages resulted in increased lymphocyte counts, improved immune functions (including antigen-specific responses), and lower toxic metabolites. Both patients are currently at home and clinically well, with normal growth and development. These results indicate the safety and efficacy of HSC gene therapy combined with nonmyeloablative conditioning for the treatment of SCID. 相似文献
39.
Employing the case of theredwood Headwaters forest in rural NorthernCalifornia, this paper investigates the extentto which an anti-corporate progressive alliancebetween labor and the environmental movement ispossible in contemporary global capitalism.Progressive alliances between labor and theenvironmental movement have been historicallydifficult. This has been particularly the casein the timber industry, where companies havebeen able to mobilize workers againstenvironmentalists' designs. The caseillustrates the events that led to the purchaseof the Headwaters Forest by the state ofCalifornia and the Federal Government fromPacific Lumber. This is a subsidiary of Maxxam,a corporation with interests in a variety ofeconomic sectors. The objective was to haltMaxxam's rampant deforestation of old growthredwood. Though conflict between labor andenvironmentalists existed, the casedemonstrates that the labor and theenvironmental movement were united againstMaxxam. They, however, were not completelysuccessful in their struggle as Maxxam greatlybenefited from the state-sponsored landpurchase. Maxxam gained economically, was ableto shape the ideological framework throughwhich the purchasing agreement was achieved,and extended its hegemony over the state. Thisoutcome cast doubts on the possibility thatcurrent forms of environmental protection couldtranscend commodified and reductionistpostures. Simultaneously, the existence of acommon anti-corporate consciousness among largesegments of labor and the environmentalmovement makes the environment a contestedterrain and allows for some optimism about thefuture of the struggle for ecologically soundsocial arrangements. 相似文献
40.
Laviano A Meguid MM Rossi-Fanelli F 《Science (New York, N.Y.)》2002,298(5593):539-41; author reply 539-41