Effect of testicular tissue lysate on developmental competence of porcine oocytes matured and fertilized in vitro

The objective of the present study was to investigate the effect of testicular tissue lysate (TTL) on developmental competence of germinal vesicle (GV) stage porcine oocytes. Two types of TTL were prepared through repeated freeze–thaw in liquid nitrogen, one from whole testicular tissue (wTTL) and other from either of four different sections of testes, namely just beneath the tunica albuginea (TA), from the transitional area between the seminiferous cord/tubules and the mediastinum testis (TR) and from the intermediate area (parenchymal tissue origin) and CE (cauda epididymis origin). The whole or section‐wise TTL treatments were given for 44 hr during in vitro maturation (IVM). Oocyte maturation was done in either of the two media, namely defined (high‐performance basic medium for porcine oocyte maturation, commercially available) and serum containing (TCM199). After maturation, oocytes were co‐incubated with fresh spermatozoa for 6 hr and then transferred to embryo culture media. Treatment of GV stage oocytes with wTTL (1 mg/ml) increased the cleavage and morula percentage rate (69.23 ± 6.23 and 48.15 ± 6.77, respectively) than that of their control (58.33 ± 8.08 and 32.54 ± 5.53, respectively) in defined media, and in serum‐containing media, cleavage and morula percentage rate were almost equal in both treatment (54.56 ± 7.79 and 34.70 ± 6.78, respectively) and control (59.52 ± 8.21 and 38.52 ± 6.54, respectively). However, effect of wTTL was not significant. In case of section‐wise TTL supplements, TR section significantly (p < .01) improved cleavage and morula rate (58.43 ± 7.98 and 36.14 ± 6.89, respectively) followed by TA. In conclusion, present study indicates that IVM, in vitro fertilization and in vitro culture of embryo are improved in the presence of TTL, particularly its TR section. Further study is expected to reveal the principal components of TTL which may prove useful for IVM.     

Antidiarrhoeal activity of Strychnos potatorum seed extract in rats

The antidiarrhoeal activity of the methanol extract of the dried seeds of Strychnos potatorum (MESP) has been evaluated out in rats using different models (castor oil-induced diarrhoea, effects on gastrointestinal motility and on PGE(2)-induced gastric enteropooling. MESP (100, 200 and 400 mg/kg, p.o.) significantly (P<0.001) inhibited the frequency of defaecation and reduced the wetness of faecal droppings in castor oil-induced diarrhoea, decreased the propulsion of charcoal meal through the gastrointestinal tract, and also reduced the PGE(2)-induced enteropooling.     

孟加拉国丘陵地带桔园和灌木林地土壤物理性质的比较评价(英文)

对孟加拉国丘陵地带Mro部落经营的桔园与灌木林35%和55%两种坡地土壤物理性质进行了比较与评价。每一块用地内挖掘3个土壤剖面采集不同深度(0-5cm,5-15cm and 15-30cm)的土壤样品,测定土壤水分、有机质含量和土壤密度。最大持水量、田间持水量、干或湿土壤容重和土壤孔隙度的测定仅限于表层土壤。两种坡地桔园3个深度的土壤水分含量均显著高于灌木林地相同深度的土壤水分。桔园在55%坡地内土壤有机质含量低于林地含量值,而在35%坡地内的含量值则高于林地值。两块样地内上述两项测定值均在表层土壤中最高,且随土层深度的增加而降低。与灌木林地比较,桔园地表层土壤最大持水量、孔隙度和3个不同深度的土壤密度均较低。两块用地内不同坡地的表层土田间持水量有所差异,且桔园地干或湿表层土壤容重高于林地内的数值。表5参23。     

Brucella suis in three dogs: presentation,diagnosis and clinical management

Brucella suis is an emerging, zoonotic disease predominantly affecting dogs and humans that engage in feral pig hunting in Australia and other countries. Although B. suis infection in dogs shares some clinical similarities to the host-adapted species (B. canis), B. suis remains an incompletely understood pathogen in dogs with limited published data on its pathogenesis and clinical features. This case series describes the presentations, diagnosis, and clinical management of B. suis infection in three dogs: (1) a bitch with dystocia, abortion and mastitis; (2) an entire male dog with septic arthritis and presumptive osteomyelitis; and (3) a castrated male dog with lymphadenitis. Unique features of these cases are reported including the first documented detection of B. suis from milk and isolation from lymph nodes of canine patients, as well as the follow-up of pups born to a B. suis-infected bitch. Consistent with previous reports, all three dogs showed a favourable clinical response to combination antibiotic therapy with rifampicin and doxycycline. Individually tailored drug regimens were required based on the clinical presentation and other factors, including owner expectations and compliance with therapy as well as a zoonotic risk assessment (generally considered low, except around time of whelping). The authors include their recommendations for the clinical management of dogs that are at-risk or seropositive for B. suis with or without clinical signs or laboratory-confirmed infection.     

Cold‐pressed rapeseed cake as a component of the diet of common carp (Cyprinus carpio L.): effects on growth,nutrient utilization,body composition and meat quality

The aim of the study was to determine the possibility of inclusion of cold‐pressed rapeseed cake (CPRC) in diets for common carp, Cyprinus carpio L. Four isonitrogenous (322 g kg^?1 crude protein) and isoenergetic diets (17.5 MJ kg^?1) with four levels of CPRC incorporation 0, 110, 220 and 330 g kg^?1 (treatments RC0, RC11, RC22 and RC33, respectively) were evaluated in a 50‐day growth test with common carp of 500 g initial weight. Statistically significant higher final weights (1215–1243 g) were obtained in fish fed diets including CPRC. Fish growth rates were nearly identical in all treatments (specific growth rate of 1.7–1.8% day^?1). Similar results were obtained in respect of feeding coefficients (FCR) that ranged from 1.6 to 1.8. Crude protein and ash concentration in fish bodies did not change. Statistically significant decreases in moisture content were found in carp in groups RC22 and RC33. Statistically significant increase of crude lipid in fish bodies were found in groups RC22 and RC33. The slaughter traits and meat quality analysis of carp did not show significant differences. We conclude that it is possible to include up to 330 g kg^?1 of CPRC in diets of 2‐year‐old common carp without significant effects on growth and food utilization.     

Yield and water use efficiency of cauliflower under varying irrigation frequencies and water application methods in Lower Gangetic Plain of India

A field study (1999-2000 to 2001-2002) was carried out to optimize the irrigation frequency and suitable water application methods for cauliflower with a view to increase curd yield (CY) and water use efficiency (WUE). Check Basin (CB), Each Furrow (EF) and Alternate Furrow (AF) methods were tested with three irrigation frequencies depending on the attainment of soil matric potential (Ψ_m) value at 0.2 m depth as: −0.03 MPa (F₁), −0.05 MPa (F₂) and −0.07 MPa (F₃). Maximum CY was recorded under F₁ and decreased by 10.4 and 31.4%, respectively under F₂ and F₃ frequencies. In contrast, WUE decreased by 9.3% from F₃ to F₁. Highest CY and WUE obtained under CB followed by EF and AF methods. Furrow application methods saved 12-24% irrigation water over CB method. Maximum soil water stress coefficient (K_s) recorded at curd development stage in comparison to other stages. Both seasonal evapotranspiration (ET_a) and yield-moisture stress index (Kys) recorded positive linear relationships with CY. Present study shows a crop response factor of 0.822 for cauliflower. In this region, cauliflower should be irrigated with check basin method at an interval of 8-10 days.     

Sulfur accumulation in Vertisols due to continuous gypsum application for six years and its effect on yield and biochemical constituents of soybean (Glycine Max L. Merrill)

Application of S through inorganic sources is recommended to the crops in the cropping system in S deficient soils of India. However, transformations of S in the soil profile following continuous gypsum application are not known. The accumulation of S into different fractions due to application of a total of 180—540 kg S ha^—1 over a six‐year‐period in soybean — wheat cropping system and its effect on the yield and quality of soybean produce were investigated in a vertisol area. About 25—38% of the S added was retained in the 90 cm depth of profile. Significant changes in the total S content in soil layers were recorded wherein a total of 360 kg S ha^—1 or more S was applied. Maximum increase in total S content was recorded in upper layers. Soluble S accumulated more in the 30—75 cm depth; whereas, proportion of sorbed fraction of S increased with depth. Build up of S in the surface layer of the profile was mostly related to the increase in organic fraction. The S accumulation in lower layers of the profile was mostly due to increase in soluble and sorbed fractions of S. Such an increase of the S content in the soil caused an increase in seed yield, uptake of S as well as content of oil and S containing amino acids, cystine, and methionine in seed of soybean crop.     

Influence of farmyard manure, inorganic fertilizers and weed control practices on some soil physical properties in a long-term experiment

Abstract. In a long-term experiment, soil physical properties were studied after 20 annual cycles of maize-wheat-fodder cowpea cropping with annual application of inorganic fertilizers and farmyard manure (FYM). Weeds were controlled by hand or by the use of herbicides. The hand weeding treatment resulted in a lowering of the bulk density of the surface layer (0-15 cm) and a significant increase in the subsurface (15–30 cm) density. Application of FYM significantly increased the soil organic carbon (OC), infiltration rate, water retention, aggregation and aggregate stability in water. Application of inorganic fertilizers had small but statistically significant effect in increasing soil OC.