Phylogenetic analysis of livestock oxacillin-resistant Staphylococcus aureus

The aim of this study was to characterize oxacillin-resistant Staphylococcus aureus (ORSA) isolates from livestock environments and meat market workers by molecular epidemiological analysis. Staphylococcal enterotoxin reversed passive latex agglutination (RPLA) and multiplex polymerase chain reactions (PCR) were used to detect enterotoxin-producing S. aureus. The molecular genetic similarity of ORSA was also compared by pulse-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). A total of 30 ORSA isolates were identified and 27 of these strains were from human sources-a higher contamination potential from human origin in the animal raising and handling field was suspected. The most common type of enterotoxin detected in this study was type B. Regarding the bacterial phylogenetic analysis of ORSA isolates, five major clusters of PFGE patterns were suggested with >80% similarity in cluster I. Seven MLST patterns were identified with the most prevalent types being ST338/ST338(slv) and ST59. Population genetic studies based on MLST have shown that major ORSA clones have emerged from six clonal complexes (CCs), with CC59 being the dominant one. In conclusion, a high prevalence of ORSA with enterotoxin type B as well as ST59 and ST338/ST338(slv) colonization was observed among livestock with human origins in this study. We suggest further tracking and comparing of the epidemiological evidence of community-acquired and hospital-acquired ORSA in human living environments and livestock-producing environments.     

烟草白粉病的研究进展

摘要：烟草白粉病是由专性寄生菌引起的烟草重要病害之一,主要危害烟草叶片,导致烟叶受损,严重时造成整株死亡,使烟草的产量和品质下降,近年来部分地区发病严重,是严重制约烟草绿色高质量发展的重要原因之一。为了了解烟草白粉病的发病全过程,本文对烟草白粉病的病原菌形态特征、发病原因、发病特征以及选用抗病品种、加强农业防治、生物防治、药剂防治措施等研究进行综述,并从抗病基因筛选、植物代谢产物开发、混合使用等提出防控建议,为烟草白粉病的绿色防控提供参考。     

Molecular epidemiological studies on foot-and-mouth disease type O Taiwan viruses from the 1997 epidemic

Sequence diversity was assessed of the complete VP1 gene directly amplified from 49 clinical specimens during an explosive foot-and-mouth disease (FMD) outbreak in Taiwan. Type O Taiwan FMD viruses are genetically highly homogenous, as seen by the minute divergence of 0.2-0.9% revealed in 20 variants. The O/HCP-0314/TW/97 and O/TCP-022/TW/97 viral variants dominated FMD outbreaks and were prevalent in most affected pig-raising areas. Comparison of deduced amino acid sequences around the main neutralizable antigenic sites on the VP1 polypeptide showed no significant antigenic variation. However, the O/CHP-158/TW/97 variant had an alternative critical residue at position 43 in antigenic site 3, which may be due to selective pressure in the field. Two vaccine production strains (O1/Manisa/Turkey/69 and O1/Campos/Brazil/71) probably provide partial heterologous protection of swine against O Taiwan viruses. The type O Taiwan variants clustered in sublineage A1 of four main lineages in the phylogenetic tree. The O/Hong Kong/9/94 and O/1685/Moscow/Russia/95 viruses in sublineage A2 are closely related to the O Taiwan variants. The causative agent for the 1997 epidemic presumably originated from a single common source of type O FMD viruses prevalent in neighboring areas.     

Effects of dietary methionine and lysine sources on nutrient digestion, nitrogen utilization, and duodenal amino acid flow in growing goats

This study investigated the effects of supplementation of various sources of Met and Lys on nutrient digestion, N utilization, and duodenal AA flows in growing goats. Four 4-mo-old Liuyang Black wether goats were used in a 4 x 4 Latin square experiment and were assigned to 4 dietary treatments: (1) control, (2) control + lipid-coated Met-Zn chelate and Lys-Mn chelate (PML), (3) control + Met-Zn chelate and Lys-Mn chelate (CML), and (4) control + dl-Met, l-Lys-HCl, ZnSO(4).7H(2)O, and MnSO(4).H(2)O (FML). Compared with control, PML reduced (P < 0.05) ruminal NH(3) concentration, urinary N excretion, and plasma urea N concentration and increased (P < 0.05) the activity of ruminal endo-1,4-beta-d-glucanase and beta-glucosidase, the duodenal flow of N, N retention (g/d as well as % of absorbed N), the duodenal flows of Met, Lys, His, Val, and total essential AA, and plasma concentrations of Lys, Val, Phe, and total essential AA. Supplementing Zn-Met and Mn-Lys chelates had similar (P > 0.05) but lesser effects on these measures compared with PML, and the effects on most of the measures were not statistically significant (P > 0.05) when compared with control. Supplementing free-form Met and Lys had no effects compared with control (P > 0.05). The results indicate that lipid coating and chelating of AA provide a protection, and to a lesser extent by only chelating, of the AA from microbial degradation in the rumen and possibly has effects on rumen fermentation, which increases MP supply. This technology could improve productive performance and be of potential benefit to ruminant production if cost-effective products are developed.     

The injury effect of oxygen free radicals in vitro on cultured pulmonary artery endothelial cells from broilers

Pulmonary artery endothelial cells (PAEC) were isolated from broilers by the method of tissue explantation. The cells were identified using morphological features and immunocytochemical staining using a specific antiserum against factor VIII related antigen. Xanthine/Xanthine oxidase (X/XO) served as the oxygen free radical (OFR) generating system. In vitro model of oxidative injury of PAEC was established based on the X/XO system. The effect of OFR on the growth and viability of PAEC was determined with methylthiazol tetrazolium (MTT) colorimetric assay. Malondialdehyde (MDA, a product of lipid peroxidation) in culture medium of PAEC was detected by a thiobarbituric acid colorimetric assay. The results showed that PAEC survive in vitro and can be subcultured for 5-6 passages. Morphological and immunocytochemical observations of cultured cells demonstrated specific characteristics of endothelial cells. PAECs were severely damaged by OFR. The viability of cells was reduced by the X/XO system, and a dose-dependent decrease in cell viability was found with increasing XO dosages. OFR promoted lipid peroxidation of PAEC and increased the MDA concentration in culture media. These results suggest that OFR can injure the endothelial cells from broiler pulmonary arteries in vitro, which confirms previous results obtained in vivo. Oxidative injury may play an important role in the pathogenesis of pulmonary hypertension syndrome in broiler.     

手性农药的环境行为研究进展

本文对手性农药在环境中的行为研究进行了综述.从手性农药环境行为研究意义、手性农药环境行为研究方法、手性农药环境行为研究现状(有机氯、拟除虫菊酯类杀虫剂、苯氧羧酸类除草剂、酰苯胺类杀菌剂、手性分子作为环境示踪物)和手性农药代谢研究发展趋势等方面进行了论述.在宏观研究环境危害的同时,建议开展微观的手性农药代谢研究.以确定其对人类及其他动植物及微生物的毒力和毒理,更好地指导手性农药的应用.     

Simulation Analysis of Spatial Distribution of Roadway′s Surrounding Rock Stress

To get the spatial distribution characteristic of roadway's surrounding rock stress, circular section roadway was taken as a case study. The solution of stress was got by using complex function method, and roadway's surrounding rock stress field was analysed after the solution of mapping space was transformed into the solution of roadway space. As a result, the visual diagram of stress field distribution of roadway's surrounding rock was obtained, which could describe intuitively stress distribution of roadway's surrounding rock at any position. Considering the influence of different radius and side pressure coefficients on surrounding rock stress field, the following conclusions have been obtainded: the peak value and direction of circular roadway's surrounding rock stress has nothing to do with the radius; when side pressure coefficient is less than 1/3,the roof can produce tensile stress; when side pressure coefficient is more than 3, the surrounding rock in two sides can produce tensile stress. And the change rule of hoop stress, radial stress, shear stress and the maximum and minimum of stress have also been generated.     

Experimental Analysis on Flexural Performance of Reinforced Concrete Beam in Salt-frost Environment

Under the salt frost environment, the flexural behavior of reinforced concrete was studied. The bearing capacity and deformation performance of the beams were analyzed by comparing with the reference beam(undamaged) and beams subjected to 25,50,75 and 100 salt freeze-thaw cycles respectively. The results showed the plane section hypothesis still was satisfied for the concrete strains of frost beam section, and the mechanical characteristics of frost beam was similar to reference; The main reason to affect the beam bearing capacity under salt freeze-thaw environment was the concrete cover scaling in the compressive zone. Secondly, it was the reduction in concrete strength, while the degradation of bond properties between rebar and concrete was not a dominated factor; the deformations of concrete beam increased mainly due to the reduction in concrete strength, the damage of concrete cover and the degradation of bond properties. In addition to the number of freeze-thaw cycles, the positions of freeze-thaw damage and the load level affected the degree of reduction in beam stiffness.     

Analysis on Dredger Fill Electrical Response Under Additive Electrical Field

With the purpose of the study on change rule of internal charge in soil specimens under differing circumstances, dredger fill, which was collected from Guangxi Beibu Gulf Economic Zone, was studied in various parameters, including the absence/presence of the applied DC electric field, different levels of field strength, soil water content, soil compactness, and degree of desalination. Moreover, through tentatively simulating the electrical response between thundercloud and earth mass, the triggered lightning mechanism of rock soil mass was explored. The results show that both additive electrical field and atmospheric electric field had influence over charge variation of soil, the impact of which would become more significant with the increasing field strength. Higher soil water content and lower degree of desalination could enhance the charge variation and conductivity of soil, whilst this action effect would remain stable when soil water content reached a certain degree. Although soil compactness make some effect for the charge distribution, its regularity is undefined so far.     

栗斑腹鸦繁殖期栖木利用

采用人为观察法对内蒙古通辽市扎鲁特旗东北部区域内繁殖期栗斑腹鹀(Emberiza jankowskii)栖木与巢的距离及双亲是否对栖木重复利用进行了研究.结果表明:栗斑腹鹀在不同繁殖时期内,其栖木与巢的距离具有极显著差异(F=130.265,自由度为2,P≤0.001),产卵期为(21.90±5.06)m,孵化期为(16.80±5.10)m,育雏期为(7.80±3.85)m,且双亲均有重复利用同一栖木的习性,在育雏期栖木的重复利用最高.