浅谈水生动物及其产品疫病的监控

随着我国水产养殖品种的增加 ,规模的不断扩大 ,养殖集约化程度的提高和国际间、区域间引种的频繁等致使水生动物疫病的发生机率不断增加。加之水生动物赖以生存的水环境污染 ,导致一些危害严重的水生动物疫病得以在全国范围内流行和扩散 ,给国家和养殖业者都造成了巨大的经济损失。养殖水产品的质量是当今国际贸易关注的焦点 ,也是我国水产品进入国际市场的一个关键。１９９７年 ,我国出口欧盟的水产品中屡次被检出副溶血性弧菌、霍乱弧菌等病菌 ,并且欧盟停止了双壳贝类和鲜活水产品对其的出口 ,也对其他水产品加工品也实行严格检疫。目前…     

黑龙江流域中俄保护区现状及展望

黑龙江地处中俄边界，在流域内中俄积极开展保护合作。本文分析了中俄在黑龙江两侧的保护区类型、保护对象，结果表明黑龙江流域的众保护区具有共同的保护对象，保护区的类型也惊人的相似。这些相同相似之处对于中俄保护合作深入发展有重要义。     

盆栽石榴养护技术要点

1、阳光充足石榴属阳性植物,喜阳光,整个生长季节要求有充足的光照。只要不缺水分,石榴是不怕强光的。光照不足必然影响植株生长,进而影响孕花孕蕾,开花结果。2、控花促长有时扦插新苗,当年就能开花结果,小苗首要的是先长“树身”,过早开花结果不利树身生长。头几年应抹去花芽,     

上虞海涂实验农场中心试验区排水系统探讨

上虞市海涂实验农场中心试验区的排水系统，能满足十年一遇的年最大２４ｈ降雨，两天内排出的要求。滨海盐土的地下水位高，农田基本建设除用浅、密的排水沟布置外，还应结合平整土地、发展灌溉以及适用的农、林技术等综合措施，才能达到治盐改土的效果。     

矸酆恐?,垩白率与糙米率、蛋白

Χ灾绷吹矸酆康挠跋焓钦?,直接影响比间接影响大。Study on the correlation between rice quality and agronomic traits of quality riceNIE Cheng rong, WEN Yu hui, WANG Yun bo, CHEN Ming zhong,LI Mei, LIU Dao quan(Agronomy Department, Foshan University , Nanhai 528231,China)The correlative analy     

Mycoplasma hyopneumoniae colonization of pigs sired by different boars

Differences in Mycoplasma hyopneumoniae colonization were evaluated in experimentally inoculated pigs sired by 3 different boars of the same genetic line. Forty-six pigs were used, including a treatment group and positive and negative control groups. The pigs were intratracheally inoculated with an M. hyopneumoniae suspension or with Friis media as a placebo. To evaluate differences in the magnitude of colonization during a 35-day period, nasal and tracheal swabs were collected weekly and tested by nested polymerase chain reaction (N-PCR). Temperature, weight and circulating antibodies were measured for 35 days. At 11 and 35 d postinoculation the pigs were necropsied and macroscopic and microscopic lesions were determined. A section of bronchus was tested by the indirect immunofluorescence test (IFAT), scanning electron microscopy (SEM) and N-PCR. The N-PCR results from the nasal and tracheal swabs showed that the pigs sired by one boar (B3) had a distinctive colonization pattern, different from that of the pigs from the other 2 boars and from the positive controls. SEM studies demonstrated that at 35 d postinoculation a higher proportion of B3 pigs had lower numbers of mycoplasmas attached to the cilia compared with B1 and B2 offspring. No significant differences were observed in temperature and weight gain among groups by ANOVA; however, with use of a 2 × 2 table, temperature differences were observed between pigs sired by boars B1 and B2 at 4 d postinoculation. No pigs seroconverted, showed gross or microscopic lesions, or had positive IFAT results. These results provide evidence of differences in patterns of colonization between pigs sired by different boars, suggesting a possible genetic effect.     

影响烟草花粉胚胎发生的某些因子——铁盐、蔗糖和腺素

本文研究了铁盐、蔗糖等培养基成份对烟草花粉胚胎形成的影响。结果表明,铁盐和蔗糖与烟草花粉胚形成有密切关系。培养基中没有铁盐,胚发育停止球形期;没有蔗糖、胚发育受阻在多细胞原胚期,不能进一步发育。培养基中仅有蔗糖,胚发育停止在鱼雷胚早期,将其转到只含铁盐的培养基中,鱼雷胚可继续发育直至成株。培养基中不附加铁盐或蔗糖,胚的发育均受阻。只有铁盐和蔗糖而无大量元素、微量元素及有机成份,即能形成胚状体并再生植株,但胚胎发生频率低,表明其它附加成份并不是没有意义的。另外,培养基中附加一定浓度的腺素对花粉胚形成是有利的,但高浓度是有害的。     

渭北旱塬油菜茎象甲药剂防治方法与效果

油菜茎象甲田间防治试验表明：５０％氧化乐果乳剂１０００倍液和２．５％溴氢菊酯乳剂４０００倍液喷雾对成虫的防治效果较好。春季始盛期喷药优于秋季。氧化乐果羊毛脂、久效磷羊毛脂和氧化乐果聚乙烯醇涂茎剂防治幼虫的效果较好。化学防治应以春季成虫出土始盛期药剂喷雾为主，辅以幼虫初期涂茎剂防治。     

I—ELISA特异诊断棘球蚴（包虫）病的研究

采用亲和层析技术纯化的兔抗棘球蚴抗体，建立了酶联免疫吸附抑制试验（Ｉ—ＥＬＩＳＡ）。取代反应和阻断反应均无非特异反应；检测健康对照血清１２６份（人３６，猪９０），均为阴性；与猪囊尾蚴病血清１３６份（人４６，猪９０）、细颈囊尾蚴病血清３８份（羊２２、猪１６）无交叉反应；对棘球蚴病血清１８３份（人６１、羊７１、猪５１）进行检测，其阳性符合率分别为８８．５％（５４／６１）、９８．６％（７０／７１）、９６．１％（４９／５１）。