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31.
The objectives were to use high performance liquid chromatography (HPLC) to validate an established method for adenine nucleotide separation in equine colonic mucosal tissue, to determine the inherent variability in the tissue and extraction method, and to determine the stability of ATP, ADP, and AMP in the tissue with time. Equine colonic mucosal tissue obtained from a single horse was immediately submersed in liquid nitrogen, and stored at -70 degrees C. Samples were lyophilized, extracted, and separated by HPLC. The limit of quantitation was 0.05 microg/mL. The coefficient of variation for the instrument was less than 10% for all nucleotides measured. When the tissue was not homogenized prior to sampling, there were significant differences in adenine nucleotide content between samples. However, when the tissue was homogenized prior to analysis, these differences were no longer significant. There was no significant decrease in ATP, ADP, or AMP content over a 54-day analysis period.  相似文献   
32.
The objective of this study was to determine if porcine reproductive and respiratory syndrome virus (PRRSV) could persist in non-pregnant sows and if persistently infected sows could transmit virus to naive contact controls. Twelve PRRSV-naive, non-pregnant sows (index sows) were infected with a field isolate of PRRSV and housed in individual isolation rooms for 42 to 56 days postinfection. Following this period, 1 naive contact sow was placed in each room divided by a gate allowing nose-to-nose contact with a single index sow. Index sows were not viremic at the time of contact sow entry. Virus nucleic acid was detected by polymerase chain reaction, and infectious virus was detected by virus isolation in sera from 3 of the 12 contact sows at 49, 56, and 86 days postinfection. All 3 infected contacts developed PRRSV antibodies. Virus nucleic acid was detected in tissues of all of the 12 index sows at 72 or 86 days postinfection. Nucleic acid sequencing indicated that representative samples from index and infected contacts were homologous (> 99%) to the PRRSV used to infect index sows at the onset of the study. This study demonstrates that PRRSV can persist in sows and that persistently infected sows can transmit virus to naive contact animals.  相似文献   
33.
The object of this investigation was to determine the outcome of limb amputation in wapiti. Medical records of 13 wapiti that underwent limb amputation were reviewed to determine age, weight, sex, injury preceding amputation, limb amputated, amputation location, length of hospital stay, complications, and outcome. Ages ranged from 3 months to 8 years and weights from 70 kg to 280 kg. All animals were female. Eleven animals sustained catastrophic long bone fractures prior to amputation. Five animals had front limb amputations and 8 had hind limb amputations. Of these animals, 1 with a front limb amputaiton and 5 with hind limb amputations survived. Postsurgical inability to stand is associated with hospitalization of > 1 day. Of the 13 wapiti reviewed, 5 with hind limb amputations have produced and reared offspring. Hind limb amputation can be considered a viable alternative to euthanasia when catastrophic orthopedic injuries occur in female wapiti.  相似文献   
34.
当压缩机内气体的流量较低时,气体容易出现旋转脱离现象,导致压缩机不能正常工作,采取增加压缩机内部液体的流量,在出口处设置单向阀防止气体倒流等措施,可有效地防止喘振的发生,保证输气管道的正常运行.  相似文献   
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压力实时监测法在管道泄漏检测中的应用   总被引:2,自引:0,他引:2  
检测输油管道漏油的方法很多,介绍了通过压力实时监测确定输油管道漏油点方法,以及实际应用情况。实践证明,通过压力实时监视确定输油管道漏油点的方法在实际输油运行中是切实可行的。  相似文献   
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陕西秦巴山区的野生果树种质资源   总被引:3,自引:0,他引:3  
  相似文献   
40.
该文探讨了不用编制削度表直接建立高精度立木干曲线的方法.当采用干曲线是3次多项式时,通常根据10分法测定树干各部位直径,用最小二乘法求出该方程式的参数.从数学角度,只要知道树干任意3个部位的直径,就可以用最小二乘法或联立方程式求解干曲线参数.本文以樟子松为例,探讨用树干哪3个部位直径拟合的干曲线最接近实际干曲线,为建立立木干曲线提供有效方法.利用9个部位半径(方法Ⅰ)和利用其中3个部位半径(方法Ⅱ,28种组合)分别拟合干曲线,结果表明方法Ⅱ的6种半径组合的精度良好,其中的3种组合,(r1.3,r0.3,r0.7),(r1.3,r0.4,r0.7)和(r1.3,r0.4,r0.8)是拟合现实干曲线的有效方法.  相似文献   
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