不同抗性苹果砧木叶片接种轮纹病菌后防御酶活性的变化

以高感砧木3-1-29-4和高抗砧木1-1-10-8为试材,对其叶片接种轮纹病菌(Botryosphaeria berengriana.f.sp.piricola),测定了接种后防御酶活性的变化。结果表明:轮纹病菌侵染后,叶片的过氧化物酶(POD)和过氧化氢酶(CAT)的活性均表现为先升后降的趋势,且抗性砧木1-1-10-8的活性高于高感砧木3-1-29-4。叶片的超氧化物歧化酶(SOD)和苯丙氨酸解氨酶(PAL)的活性在接种后持续升高。接种后,抗病砧木1-1-10-8的多酚氧化酶(PPO)活性先升高后降低,高感砧木3-1-29-4的PPO活性持续升高。     

Analysis of genetic variation in eggplant and related Solanum species using sequence-related amplified polymorphism markers

Collection and characterization of all sorts of germplasm resources are required for the development of new cultivars. Molecular characterization is more reliable than morphological characterization. Here, we employed sequence-related amplified polymorphism (SRAP) markers to evaluate genetic variation in a diverse collection of 56 Solanum accessions. Fifty-five SRAP primer combinations were used and a total of 635 polymorphic bands were observed. Cluster analysis by the unweighted pair-group method with arithmetic averages based on similarity matrices indicated that there were three clusters: (i) S. melongena; (ii) S. aethiopicum; (iii) S. surattense. The coefficients of genetic similarity among all the accessions ranged from 0.04 to 0.96 with an average of 0.73, and averaged 0.78 among S. melongena accessions originated from China, indicating extensive genetic variation. These results demonstrated that SRAP can be efficiently used to estimate genetic diversity and analyze phylogenetic relationship.     

白檀未成熟胚的器官发生和植株再生

以白檀未成熟胚为试材,以改良MS为培养基,研究了白檀未成熟胚的器官发生和植株再生.结果表明:改良MS(mMS)能够很好的诱导愈伤组织,在添加了0.2 mg/L 6-BA+0.1 mg/L NAA的培养基中,诱导率高达92.5％;胚性愈伤组织分化最佳培养基为mMS+0.25 mg/L 6-BA+0.15 mg/L NAA,分化率为72.4％;分化出的胚性愈伤组织在空白mMS培养基上继代培养15 d,76.6％的组织分化出芽,再转入1/2mMS+ 1.5％蔗糖培养基上培养20 d,芽长至1.5 cm.     

60Co-γ辐射对茭白叶片光合特性和荧光参数的影响

分别以50,75和100Gy剂量水平的60Co-γ射线对3叶期的茭白(Zizania latifolia)幼苗进行辐射处理,通过测定其叶绿素含量、光合气体交换指标和叶绿素荧光参数来探讨γ射线辐射对茭白植株光合和荧光生理特性的影响.试验结果表明,75 Gy剂量处理茭白的叶绿素a、叶绿素b和总叶绿素含量降低显著;不同剂量的...     

不同基因型的青菜、白菜耐镉性差异研究

通过溶液培养,对重金属镉(Cd)处理下不同品种的青菜和白菜地上部可食部分的生物量以及对镉的吸收和积累特点进行了比较研究.结果表明:在50μmol/L的Cd处理下,青菜和白菜的生长均受到不同程度的抑制作用,其地上部的生物量均显著低于对照,青菜对Cd具有较强的敏感性,其相对生物量均下降到对照的50%以下,白菜对Cd的耐性高于青菜;镉处理下,不同品种的青菜和白菜对镉的吸收积累具有显著性差异,青菜单位重量的Cd含量明显高于白菜;对青菜和白菜进行了镉耐性和敏感性品种筛选,为无公害蔬菜的种植及镉污染土壤的植物修复提供理论参考.     

Effect of Ginkgo biloba extract on the expression of connective tissue growth factor in the initiating stage of fibrosis in lungs of rats

AIM: To study the effect of Ginkgo biloba extract (GbE) on the expression of connective tissue growth factor (CTGF) in the initiating stage of pulmonary fibrosis of rats after administration of bleomycin (BLM).METHODS: The expression of CTGF in lungs was detected by Western blotting. The content of hydroxyproline was assayed by the method of chloramines T. The content of malondialdehyde (MDA) in plasma was investigated by colorimetry.RESULTS: On day 14 after administration of BLM, the contents of CTGF in lungs and MDA in plasma in BLM+NS group were higher than those in NS group, respectively (P<0.05; P<0.01). On day 30 after BLM, the contents of hydroxyproline in lungs and MDA in plasma in BLM+NS group were higher than those in NS group, respectively (both P<0.01). Treatment with GbE ameliorated the above changes induced by BLM. CONCLUSION: GbE ameliorates the up-regulation of CTGF in the initial stage of fibrosis in lungs of rats after administration of BLM. GbE prevents the hyperoxidative injury in lungs of rats after BLM, which might be one of mechanisms underling the effect of GbE on CTGF.     

套袋对黄瓜产量和品质的影响及经济效益分析

以"中农16号"为试材,研究套袋对黄瓜生长和品质的影响,探讨套袋黄瓜预期产生的经济效益。结果表明:套袋可显著抑制弯瓜及畸形瓜的产生,增产效果显著;套袋黄瓜平均长度、平均重量、单位重量分别比对照组高9.83%4、2.99%、29.94%;套袋处理对黄瓜的水分含量和瓜皮表面色泽无显著影响;套袋处理可显著提高黄瓜糖酸比,改善黄瓜口感风味;套袋黄瓜VC和可溶性蛋白含量分别比对照提高了45.80%和16.82%;套袋黄瓜预期产生的经济效益远远大于套袋所消耗的薄膜材料以及人工费用。     

不同落叶提取液对豇豆种子化感效应的研究

以豇豆种子为受体材料,研究5种落叶提取液对豇豆种子的化感作用.结果表明:不同浓度的落叶提取液对豇豆种子发芽和幼苗的生长有不同的影响,落叶提取液对种子萌发表现出高浓度抑制、低浓度促进的现象.对幼苗的生长有一定的促进作用,小叶榕、黄桷兰落叶提取液不同程度地抑制豇豆的干物质重,且随着浓度的增加,抑制作用逐渐增强;桂花落叶提取液对豇干物质重的影响呈现出低浓度促进、高浓度抑制现象.     

Advances in prevention of nonsteroidal anti-inflammatory drug-induced gastropathy by proton pump inhibitor

Nonsteroidal anti-inflammatory drugs (NSAIDs) are powerful in anti-inflammatory, analgesic and antirheumatic effects, and widely used in treating the corresponding diseases. Over the years, many new dosage forms and structures of NSAIDs appear since aspirin was developed 112 years ago. However, the universal use of NSAIDs produces unavoidable mucosal lesions in gastrointestinal tract. As yet, proton pump inhibitor (PPI) has been used in the treatment of gastropathy induced by NSAIDs. This article will focus on the advances in prevention of NSAIDs-induced gastropathy by proton pump inhibitor.     

杨梅简易气调保鲜过程中气体成分变化及保鲜效果

以东魁杨梅为试材,研究简易自发气调贮藏(MA)和充氮气调贮藏(NA)条件下,杨梅贮藏小环境中气体成分的变化和对贮藏效果的影响。结果表明:在MA中,乙烯浓度在2d达到高峰;O2前4d浓度急剧下降,后维持在2.48%至1.78%;CO2一直呈上升趋势,试验过程浓度提高了124.3%。在NA中,乙烯高峰出现时间明显推迟、峰高降低;O2浓度维持在2.28%-1.82%,CO2变化与MA相似,浓度提高了131.3%。MA和NA均有效延缓杨梅鲜果的软化变质,减缓果实硬度和总糖的下降速率。贮藏至16d,NA和MA中果实变质率分别比对照下降31.6%和22.5%,硬度比对照增加25.93%和21.98%,总糖含量处理与对照无明显差异。