不同品种无核葡萄在贮藏过程中品质及生理变化研究

以"无核白"、"费雷无核"、"克瑞森无核"3个无核葡萄品种为试材,研究了在保鲜剂处理后低温(-1～0℃)贮藏期间各品种果实品质及生理变化情况,以期筛选出耐贮品种。结果表明:"克瑞森无核"在整个贮藏过程中好果率下降幅度、失重率和果梗褐变指数上升幅度显著低于其它2个品种,品质生理指标和生理代谢变化小,表现出良好的耐贮性,适合长期贮藏。     

Effects of astragaloside on the levels of sex hormone and oxidative stress injury in rats with polycystic ovary syndrome

AIM To investigate the effects of astragaloside on the levels of sex hormone and oxidative stress in rats with polycystic ovary syndrome （PCOS）. METHODS Female SD rats （n=60） were randomly divided into normal control group, model group, Diane-35 （0.339 2 mg/kg） group, low dose astragaloside （12.5 mg/kg） group and high dose astragaloside （50 mg/kg） group, with 12 rats in each group. The PCOS model was induced by letrozole （1 mg/kg）, which was administered by gavage once a day for 3 weeks. After administration, the estrus cycle of the rats was observed by vaginal smear, and the ovarian index was calculated. HE staining was used to observe the histopathological changes of the ovaries. Serum levels of the sex hormones testosterone （T）, estradiol （E₂）, luteinizing hormone （LH） and follicle-stimulating hormone （FSH） were measured by ELISA. The levels of superoxide dismutase （SOD）, malondialdehyde （MDA） and glutathione peroxidase （GSH-Px） in serum and ovarian tissue were detected by colorimetry, and the protein levels of steroidogenetic acute regulatory protein （StAR） and apoptosis-related proteins cleaved caspase-3, Bax and Bcl-2 in ovarian tissue were detected by Western blot. RESULT Compared with control group, the oestrous cycle of the rats in model group was disorder, and the ovarian index was increased, ovary was polycystic. The serum levels of T, LH and MDAwere significantly increased （P<0.05）, while the contents of E2, FSH and the activities of GSH-Px and SOD were significantly decreased （P<0.05）. The levels of MDA, StAR, cleaved caspase-3 and Bax proteins in ovarian tissue were significantly up-regulated （P<0.05）. GSH-Px and SOD activities and Bcl-2 protein levels were significantly down-regulated （P<0.05）. CONCLUSION Astragalosideeffectively balances the levels of sex hormone in PCOS rats and relieves the oxidative stress injury, the mechanism may be related to the inhibition of StAR expression.     

不同番茄品种氮素吸收利用的差异

以吉林省市场上常见的25个番茄品种为材料,研究了同一氮素水平不同番茄品种氮素吸收利用差异。结果表明：番茄总吸氮量和氮素利用效率的变幅分别为262.8～460.1 kg?hm-2和281～526 kg?kg-1。聚类分析表明,吸氮能力较弱、氮素利用效率较高、产量高的品种有4个：朝研219、瑞琦1号、四季粉红、佳粉19,产量平均值为145 754 kg?hm-2。相关分析及通径分析表明,总吸氮量与番茄产量呈显著正相关,并对提高番茄产量起主导作用。     

早熟耐贮浓黄皮甜瓜品种黄子金玉的选育

黄子金玉是针对目前国内市场栽培面积较大、黄皮、厚薄皮杂交品种丰甜1号类型选育的新型甜瓜品种,通过6代定向选育,结合商品性及抗病力筛选而成的新杂交种。该品种具有黄子标记、抗病能力强、易栽培、极早熟和商品性好等优点;植株生长势强,苗期即比同类品种生长快,叶色深,茎干粗壮,适应性强。     

设施秋延迟番茄主要农艺性状与产量的相关通径分析

对16个番茄品种的13个农艺性状和产量进行了相关和通径分析.试验结果表明,单株产量和总采果数、株高、叶片数相关性达显著水平,相关系数分别为0.538 6,0.614 4和0.518 2.回归和通径分析结果表明,早期采果数、平均单果质量、果肉厚、株高、总采果数对单株产量直接作用较大,早期采果数通过总采果数、第1稳果数、平均单果质量、株高、茎粗、果肉厚对单株产量间接作用均为负值且较大.     

Response of CREG1 promoter to serum starvation in human vascular cells

AIM: In order to explore the regulatory mechanisms of the human cellular repressor of E1A-stimulated genes (hCREG1) in vascular smooth muscle cells (VSMCs)-HITASY and in human umbilical vein endothelial cells (HUVECs), we clone and construct hCREG1 promoter vector to detect its expression in different vascular cells. METHODS: With the results of biological information, the fragments including -3 677 bp, -2 310 bp and -945 bp upstream sequences of hCREG1 were amplified respectively using human genomic DNA template by PCR. The products were inserted into pMD18-T vector, and then were subcloned into pEGFP-1 report vector to obtain the pEGFP-hCREG1-promoter vectors. The pEGFP-hCREG1-P3677, pEGFP-hCREG1-P2310 and pEGFP-hCREG1-P945 vectors were transfected into HITASY cells and HUVECs transiently and the expression of green fluorescent protein (GFP) was detected respectively by Western blotting and fluorescent microscope. RESULTS: It was confirmed that all three PCR fragments inserted into vectors were corrected by sequencing analysis. However, the expression of GFP was different significantly in both types of vascular cells. The expression of GFP in HUVECs was higher than that in HITASY cells. Meanwhile, the expression of GFP in HITASY cells with 0.5% FBS was increased obviously compared to that in HITASY cells with 10% FBS. CONCLUSION: The reporter vectors of hCREG1 promoter are constructed successfully in which the core promoter region might be located in -945 bp-0 bp of 5′ upstream sequences. This study will provide an experimental basis for exploring the regulation of hCREG1 expression.     

Role of gap junction in pentylenetetrazol-induced epilepsy in rats

AIM: To study the role of gap junction(GJ) in the pathogenesis of epilepsy. METHODS: The expression of connexin(Cx) 32 and Cx43 was evaluated in penlylenetetrazol(PTZ)-induced epilepsy rats at different time points with immunohistochemistry and real-time RT-PCR. The uncoupler of gap junction,carbenoxolone (CBX), and anti-epilepsy drug,carbamazepine (CBZ), were introduced to investigate the role of gap junction in epilepsy. RESULTS: The expression of Cx32 in the cortex and hippocampus of rats increased 2 h after PTZ administration, and was more obvious 8 h later, so was the expression of Cx43. CBX not only notably inhibited the expression of Cx32 and Cx43, but also suppressed the seizures. CBZ had no obvious effect on Cx32/43 expression. Real-time RT-PCR examination showed that the level of Cx32 mRNA went up rapidly 2 h after seizures, and began to decrease 10 h later. The level of Cx43 mRNA in PTZ group was higher than that in control group from 2 h to 5 h. CBX inhibited both the seizures and the increase in the mRNA expression of Cx32/43, while CBZ only suppressed the seizures and did not affect the expression of Cx32/43. CONCLUSION: GJ between neurons is reinforced after epileptic activities and takes part in the pathophysiological mechanism of synchronization and epilepsy. CBZ has no effect on the expression of Cx32 and Cx43, indicating that the anti-epileptic mechanism of CBZ is independent of GJ.     

黄瓜叶面积测量方法的评价

采用方格纸法、等腰三角形法及数码相机拍照法测定黄瓜叶面积。结果表明,采用这3种方法测定的结果差异不显著。综合考虑耗时、信息量、先进性等因素,数码相机拍照法为最优测定方法。     

解淀粉芽孢杆菌抗菌脂肽研究进展

解淀粉芽孢杆菌作为益生菌已被广泛应用于生物防治领域,其产生的抗菌脂肽在植物病害防控上发挥重要作用,具有绿色、安全、高效等优势。该研究从抗菌脂肽类型、抗菌脂肽合成酶相关基因、抗菌脂肽生防作用及抑菌机理、通过突变菌株的选育和发酵工艺提高抗菌脂肽产量等方面对解淀粉芽孢杆菌抗菌脂肽进行了综述,同时对其发展前景进行了展望,以期为植物病害的生物防控提供参考依据。     

紫花苜蓿叶片对硒胁迫的生理响应研究

以紫花苜蓿"大叶苜蓿""维多利亚""阿尔冈金"为试材,采用土培法,研究了硒胁迫对3种紫花苜蓿叶片酶促防御系统的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)和抗坏血酸过氧化物酶(APX)活性及丙二醛(MDA)、可溶性蛋白质含量的影响。结果表明:在100μmol·L-1Se时,硒胁迫显著提高了3种紫花苜蓿叶片SOD、APX、POD、CAT活性和可溶性蛋白质含量。Se为900μmol·L-1时,SOD、APX活性和可溶性蛋白质、MDA含量显著增加,而CAT、POD活性显著降低。不同品种间抗氧化酶活性相比,"大叶苜蓿"抗氧化酶SOD、APX、POD、CAT活性显著高于其它2个苜蓿品种,且差异显著。综合评价表明,"大叶苜蓿"的抗氧化能力最强,其次为"阿尔冈金","维多利亚"苜蓿的抗氧化能力最差。