气吸滚筒式排种器种盘参数对种子分布规律的影响

种盘的结构参数和工作参数直接影响种子的运动状态,从而影响吸种效果,通过对种盘的振动试验分析,为进一步提高气吸滚筒式排种器种盘前端种子分布的均匀性,该文以气吸滚筒式排种器种盘为试验对象,研究了种盘与滚筒角度、激振力加载位置、振动器压力对种子在种盘前端吸种区域分布规律的影响。采用3因素3水平实验设计,得出了种子分布与三个因...     

苹果杂交后代植株果实着色性状的遗传分析和预测

苹果着色问题是影响我国许多产区苹果质量的重要因素,本研究利用苹果着色相关基因MdMYB1（GenBank登录号：DQ886414）设计引物进行PCR扩增,开发出一个酶切位点为HaeⅢ的CAPS标记Mb2,该标记可用于区分杂交亲本‘富士’和‘嘎拉’苹果及分析其杂交后代植株。进一步利用Mb2标记对该杂交组合的后代植株进行遗传分析,结果表明,可将77个后代植株分为2组,一组42个后代植株含有来自‘嘎拉’亲本的非红色性状等位基因以及来自‘富士’亲本的非红色性状等位基因或者红色性状等位基因,其果实颜色预测为非红色与红色或者非红色与桔红色;另一组35个后代植株含有来自‘嘎拉’亲本的红色性状等位基因以及来自‘富士’亲本的非红色性状等位基因或者红色性状等位基因,其果实颜色预测为桔红色与红色或者只有红色。本研究结果将为选育优质苹果新品种提供依据和方法参考。     

对高职物业管理专业校企合作人才培养模式的实践探索

高职教育校企合作人才培养模式不仅可以满足高职院校培养具有全面素质、高技能人才的需求,也是解决高职毕业生就业难的有效途径。以物业管理专业为例,论述了校企合作人才培养模式的具体实践,提出了符合高职教育特色的校企合作方式。     

Impact of physicochemical parameters on the decomposition of deoxynivalenol during extrusion cooking of wheat grits

Deoxynivalenol (DON) is a toxic secondary metabolite produced by molds of the Fusarium genus and is known to cause a spectrum of diseases in animals such as vomiting and gastroenteritis. It is found in cereals and cereal products as most processing techniques lead only to a partial reduction of deoxynivalenol levels. One technique with a reported relatively high impact on deoxynivaleol decomposition is extrusion cooking. In the current work, systematic studies of a range of physicochemical parameters, such as temperature, moisture, compression, residence time in the extruder, pH value, and protein content, on their impact on deoxynivalenol decomposition during extrusion cooking were performed. The analysis of deoxynivalenol was made by high-performance liquid chromatography--tandem mass spectrometry using a quick, easy, cheap, effective, rugged, and safe-based cleanup with 15-d(1)-deoxynivalenol as an internal standard. It could be shown that the reduction of deoxynivalenol levels is dependent on a set of parameters partially interacting with each other. Especially the moisture content and compression are key factors for the reduction of deoxynivalenol levels. A correlation between residence time of the mycotoxin in the extruder and deoxynivalenol degradation was also observed when screws without a compression factor were used. Generally, the reduction of deoxynivalenol levels was increased by the use of screws with a high compression factor. As known from cooking, deoxynivalenol could also be easily degraded by extrusion under alkaline conditions. Furthermore, an increase of the protein content of the starting material resulted in higher reduction rates of deoxynivalenol.     

Growth kinetics of amyloid-like fibrils derived from individual subunits of soy β-conglycinin

The amyloid-like fibrillation of soy β-conglycinin subunits (α, α', and β) upon heating (0-20 h) at 85 °C and pH 2.0 was characterized using dynamic light scattering, circular dichroism (CD), binding to amyloid dyes (Thioflavin T and Congo red), and atomic force microscopy. The fibrillation of all three subunits was accompanied by progressive polypeptide hydrolysis. The hydrolysis behaviors, fibrillation kinetics, and morphologies of amyloid-like fibrils considerably varied among α, α', and β subunits. Faster hydrolysis rates and special fragments were observed for the α and α' subunits compared to the β subunit. However, the order of the fibrillation rate and capacity to form β-sheets was α' > β > α, as evidenced by CD and Thioflavin T data. Moreover, sequential growth of twisted screw-structure fibrils, leading to macroscopic fibrils with distinct morphological characteristics, was observed for β-conglycinin and individual subunits. The different fibrillation kinetics and morphologies of α, α', and β subunits appear to be associated with the differences in the amino acid composition and typical sequence of peptides. Besides, the disruption of ordered structure of fibrils occurred upon further heating (6-20 h) due to extensive hydrolysis. These results would suggest that all subunits are involved in the fibrillation of β-conglycinin, following multiple steps including polypeptide hydrolysis, assembly to amyloid structure, and growth into macroscopic fibrils with a fibril shaving process.     

喀斯特石漠化综合治理区表层土壤有机碳密度特征及区域差异

以中国南方喀斯特地区具有代表性的贵州为例,选取3个典型喀斯特石漠化综合治理示范区,以划分出的4条核心小流域为监测单元,对各示范区内及示范区之间不同等级石漠化样地表层土壤有机碳密度特征进行比较。结果表明:各示范区之间表层土壤有机碳密度变异性较大,表现为红枫湖示范区(6.12 kg m-2)>鸭池示范区(5.21 kg m-2)>花江示范区(2.80 kg m-2);不同等级石漠化样地之间土壤有机碳密度整体表现为无明显、潜在石漠化样地大于中、强度石漠化样地;岩石裸露率是影响喀斯特地区表层土壤碳密度的重要影响因子之一,特别对于中、强度石漠化区;地貌、气温和不同工程措施也在一定程度上影响到土壤有机碳密度特征。喀斯特石漠化综合治理具备巨大的固碳减排效应,采取有效措施增加有机碳蓄积量是今后石漠化综合治理的关键。     

SYBR Green I荧光定量PCR法检测朗德鹅填饲前后黑素皮质素受体-4基因(MC4R)的表达

黑素皮质素受体4基因(MC4R)在各组织中的不同表达对于鹅脂肪代谢具有重要作用.本研究应用强制填饲技术建立腹型肥胖朗德鹅(Casuarius casuarius)模型,提取填饲后及正常朗德鹅新鲜腹脂、肝脏等13种组织的总RNA,SYBR GreenI荧光定量PCR法检测各组织MC4R基因的表达,选择β-actin作为内参基因,使用半定量2-△△Ct法分析.PCR结果显示,MC4R基因及β-actin在填饲前后朗德鹅心脏、肝脏、脾脏、肺、肾脏、肌胃、小肠、胰腺、脑、胸肌、腿肌、腹脂、皮下脂等13种组织中均存在不同程度表达.SYBR Green I荧光定量PCR结果表明,在肾、小肠和心这3个组织中,填饲后朗德鹅MC4R表达量均比对照组中朗德鹅该基因表达量低,填饲后分别为填饲前表达量的0.41±1.80、0.65±5.75和0.72±1.22倍.而在其他组织中,MC4R基因表达量均有不同程度的增加,填饲组/对照组相对表达量倍数由高到低顺序分别为:22.78±1.00倍(脑),9.08±2.80倍(肝),5.28±1.83倍(肺),3.78±3.01倍(胰),3.07±3.64倍(腿肌),2.90±0.97倍(胃),2.34±1.66倍(皮下脂肪),2.18±3.01倍(腹脂),2.07±0.37倍(胸肌),2.01±1.75倍(脾).结果符合MC4R作用机理,为畜牧生产及人类肥胖问题的研究提供间接依据.     

黄土坡面细沟径流输沙对水动力学参数的响应

细沟径流输沙是细沟侵蚀产沙的重要过程,阐明细沟径流输沙与水动力学参数之间的关系可以有效地揭示细沟径流输沙的动力学机制,并为建立细沟侵蚀过程物理模型奠定基础。采用具有定流量人工放水的组合小区模拟降雨试验,研究黄土坡面细沟径流输沙对水动力学参数的响应关系。结果表明:1)一次降雨径流产生的细沟输沙模数对水动力学参数平均值响应关系大小顺序为平均水流断面单位能量(R=0.99)>平均水流功率(R=0.88)>平均水流切应力(R=0.82)>平均单位水流功率(R=0.76);2)降雨径流过程中,细沟输沙率对水动力学参数瞬时值响应关系为水流切应力(R=0.88)>水流功率(R=0.47),水流断面单位能量和单位水流功率的相关性较差,细沟输沙率对水流切应力的响应关系呈幂函数方程关系。     

乡镇规划调控下的农村居民点空间布局优化

为科学确定农村居民点空间布局优化类型,有效指导农村居民点空间布局优化及新农村建设实践。该文以武川县可可以力更镇为例,在乡镇规划调控的前提下,按农村居民点不同演化方向,将位于城镇建设用地扩展边界范围内的农村居民点确定为城镇化型,位于扩展边界范围外的农村居民点确定为非城镇化型。针对城镇化型农村居民点,因其未来演化方向而将其空间布局优化类型确定为社区化管理型;针对非城镇化型农村居民点,从乡镇总规协调度、生产生活便利度、居民点活跃度3大方面构建农村居民点斑块综合影响力评价指标体系,评价其综合影响力,并据此将其空间布局优化类型划分为综合发展潜力型、原地整治保持型、迁村并点扶持型3类。结果表明,可可以力更镇确定为社区化管理型的农村居民点6个,面积15.29 hm2;综合发展潜力型46个,面积178.28 hm2;原地整治保持型53个,面积189.20 hm2;迁村并点扶持型40个,面积141.22 hm2,并针对4种农村居民点空间布局优化类型提出较为具体的优化方案。研究结果符合可可以力更镇实际情况,能为农村居民点空间布局优化与整治提供新的思路。     

引江济淮工程河道边坡膨胀土开裂规律及影响因素

为合理确定膨胀土边坡加固措施,需研究膨胀土在自然条件下的土体裂隙演化规律。该文结合引江济淮试验工程,就新开挖暴露的膨胀土边坡进行裂隙开展观测试验,定量分析在自然条件下膨胀土边坡裂隙开展的影响因素。结果表明:膨胀土边坡表面裂隙率主要受含水率控制;初次脱湿情况下,土体表面裂隙率与含水率呈线性负相关关系,且随暴露时间增加,土体表面裂隙率发展存在一个近似极限稳定值,降雨短暂影响表面裂隙发展过程;膨胀土裂隙沿深度方向的开裂速率与大气温度和大气湿度分别呈现正、负相关关系;坡比对裂隙深度的开展存在影响,主要发生在土体开裂初期;单条裂隙沿深度方向呈现"V"字型状态,相同失水条件下,膨胀性高的土体开裂深度大。