Low levels of haptoglobin and putative amino acid sequence in Taiwanese Lanyu miniature pigs

Porcine haptoglobin (Hp) is an acute phase protein. Its plasma level increases significantly during inflammation and infection. One of the main functions of Hp is to bind free hemoglobin (Hb) and inhibit its oxidative activity. In the present report, we studied the Hp phenotype of Taiwanese Lanyu miniature pigs (TLY minipigs; n=43) and found their Hp structure to be a homodimer (beta-alpha-alpha-beta) similar to human Hp 1-1. Interestingly, Western blot and high performance liquid chromatographic (HPLC) analysis showed that 25% of the TLY minipigs possessed low or no plasma Hp level (<0.05 mg/ml). The Hp cDNA of these TLY minipigs was then cloned, and the translated amino acid sequence was analyzed. No sequences were found to be deficient; they showed a 99.7% identity with domestic pigs (NP_999165). The mean overall Hp level of the TLY minipigs (0.21 +/- 0.25 mg/ml; n=43) determined by enzyme-linked immunosorbent assay (ELISA) was markedly lower than that of domestic pigs (0.78 +/- 0.45 mg/ml; p<0.001), while 25% of the TLY minipigs had an Hp level that was extremely low (<0.05 mg/ml). In addition, the initial recovery rate (first 40 min) in the circulation of infused fluorescein isothiocyanate (FITC)-Hb was significantly higher in the TLY minipigs with extremely low Hp levels than those with high levels. This data suggests that the low concentration of Hp-Hb complex is responsible for the higher recovery rate of Hb in the circulation. TLY minipigs have been used as an experimental model for cardiovascular diseases; whether they can be used as a model for inflammatory diseases, with Hp as a marker, remains a topic of interest. However, since the Hp level varies significantly among individual TLY minipigs, it is necessary to prescreen the Hp levels of the animals to minimize variation in the experimental baseline. The present study may provide a reference value for future use of the TLY minipig as an animal model for inflammation-associated diseases.     

北京油鸡与贵妃鸡杂交效果研究

本研究以北京油鸡和贵妃鸡为素材,比较北京油鸡♂×北京油鸡♀(YY)、贵妃鸡♂×贵妃鸡♀(GG)、北京油鸡♂×贵妃鸡♀(YG)、贵妃鸡♂×北京油鸡♀(GY)4种组合的外貌、体重、屠宰性能和肉质性状.结果表明:杂交后代凤头、胡须、毛腿、双五趾个体比例分别是99.4％、88.1％、95.2％和72.0％;6～20周体重显著高于贵妃鸡(P＜0.01),16周屠宰率、半净膛率、全净膛率和胸肌率高于北京油鸡(P＜0.01).以北京油鸡为母本的杂交组,同周龄体重有高于北京油鸡为父本杂交组的趋势,且屠宰率和胸肌率较高(P＜0.05),为最佳组合.     

套式PCR在猪流行性腹泻病毒感染病例诊断中的应用

猪流行性腹泻是对养猪业危害较大的一种疾病,导致腹泻的原因多种多样,给临床诊断带来了困难。本研究将套式PCR技术成功应用于猪流行性腹泻临床病例的快速诊断中,旨在为猪流行性腹泻临床病例的快速鉴别诊断提供一种新的思路。     

禽致病性大肠杆菌pilA基因的克隆及其在乳酸菌中的表达

根据GenBank中禽致病性大肠杆菌pilA基因序列设计合成1对引物,以本实验室分离的禽致病性大肠杆菌基因组DNA为模板,采用PCR技术扩增得到pilA基因片段,经测序鉴定准确后将其克隆到乳酸乳球菌表达载体pMG36e中,构建重组质粒并将其电转入乳酸乳球菌MG1363,得到重组乳酸乳球菌。SDS-PAGE分析显示,表达的蛋白约为19 ku,与预期相符。Western blot进一步证实了该蛋白的免疫反应性。     

鸡肠炎沙门氏菌外膜蛋白F的原核表达及免疫原性分析

根据GenBank中收录的鸡肠炎沙门氏菌OmpF基因序列设计1对引物,以鸡肠炎沙门氏菌辽宁分离株基因组为模板,扩增鸡肠炎沙门氏菌OmpF基因,用IEDB Analysis Resource在线预测分析,该基因可能存在15个线性B细胞抗原表位;同时将该基因克隆至原核表达载体PGEX-4T-1中,构建重组表达质粒PGEX-4T-1-OmpF,转入大肠杆菌BL21中,获得OmpF重组蛋白。Western-blot检测结果表明,该重组蛋白的分子质量约为66 kD(含标签蛋白),能被鸡沙门氏菌阳性血清识别,初步证实该蛋白具有免疫原性,为鸡肠炎沙门氏菌亚单位疫苗的进一步研究和开发奠定了理论基础。     

家蚕种茧育稚蚕人工饲料育的比较试验

通过对日系种茧育稚蚕人工饲料育繁育的一代杂交种进行比较试验。探讨人工饲料在种茧育上的应用可能性。     

两个海大香肉鸡品系屠宰性能与肉质性状的比较

以在相同条件下饲养的72日龄海大香Ⅱ号鸡、海大香特Ⅱ号鸡为研究对象,测定其屠宰性能及部分肉质指标.结果表明:两品系产肉性能均良好,Ⅱ号鸡和特Ⅱ号鸡的屠宰率平均为90.99%及89.72%,全净膛率平均为69.90%及67.78%;无论在两品系间还是性别间,肌间脂宽、皮下脂厚、腹脂率以及胸腿肌含脂率等各项脂肪指标皆差异不显著(P＞0.05);特Ⅱ号鸡(慢速生长)的脂肪沉积能力要比Ⅱ号鸡(中速生长)高;两品系的胸腿肌肉含脂率均表现为母鸡比公鸡高的趋势;无论在两品系间还是性别间,胸腿肌失水率及蒸煮损失率皆差异不显著(P＞0.05);同一样本中,胸肌失水率大于腿肌,腿肌的蒸煮损失率大于胸肌.     

目前畜禽规模养殖存在的问题调查及探讨

随着新一轮西部大开发的开始,关中-天水经济区规划的实施,国家对畜牧业投入的增加,各项相关惠农政策的到位,全区发展规模养殖的积极性空前高涨,养殖小区、专业户、专业村如雨后春笋般涌现,规模化生产模式逐步取代了传统一家一户的散养模式。规模养殖已成为畜牧业发展的主力军,与此同时,我区规模养殖场也存在着一些不容忽视的问题,主要是无序建场,养殖规模小,投资不到位和防疫、无害化处理设施缺乏。笔者就天水市秦州区规模养殖场存在的问题,结合天水市规模化畜禽养殖场(小区)动物防疫暨标准化生产实施意见要求,进行了专项调查,提出可行性建议,以期对规模养殖的健康发展提供参考。     

Studies on the pathogenicity ofBabesia bovis in water buffaloes after cryopreservation and resuscitation

Tropical Animal Health and Production - Packed erythrocytes infected withBabesia bovis were mixed with an equal volume of 16% dimethyl sulphoxide (DMS0) in Alsever’s solution and dispensed...     

略论我国草地资源综合评价的基本内容

草地资源综合评价是以草地自然植被，生态环境条件，草地资源经济和社会因素等为特性的草地利用、管理、保护等综合鉴定，在实践中草地资源综合评价与农牧业生产紧密联系的，这对合理利用草地，保护草地环境起到了重大的作用，它推动农、牧、林业生产的持续发展。到目前为止，草地资源综合评价工作，无论在理论上和方法上还缺乏系统性的论述。本文着重从三个方面的问题略加阐述，以便将草地资源评价的研究推向一个高层次，促进草地科学更全面、更系统地向前发展，进一步丰富草地学内容