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81.
姜瘟病是中国生姜生产上发生较为普遍的病害,是由青枯劳尔氏菌(Ralstoniasolanacearum)引起的。此病原菌寄主范围广,可侵染44个科300多种植物。菌体短杆状,在TZC选择性培养基上培养出现白边较宽和白边较窄的两种菌落。菌系划分为5个生理小种和5个生化型,中国的姜瘟菌主要属生理小种1号,生物型属II、III、IV。此病害是一种较难防治的土传病害,在高温、高湿、雨水多、氮肥过量的情况下发生较为严重。中国植病工作者对此病害作了大量的研究工作,对中国姜瘟病的病原菌、发病规律、及防治等研究进展作一综述。 相似文献
82.
Differences in Mycoplasma hyopneumoniae colonization were evaluated in experimentally inoculated pigs sired by 3 different boars of the same genetic line. Forty-six pigs were used, including a treatment group and positive and negative control groups. The pigs were intratracheally inoculated with an M. hyopneumoniae suspension or with Friis media as a placebo. To evaluate differences in the magnitude of colonization during a 35-day period, nasal and tracheal swabs were collected weekly and tested by nested polymerase chain reaction (N-PCR). Temperature, weight and circulating antibodies were measured for 35 days. At 11 and 35 d postinoculation the pigs were necropsied and macroscopic and microscopic lesions were determined. A section of bronchus was tested by the indirect immunofluorescence test (IFAT), scanning electron microscopy (SEM) and N-PCR. The N-PCR results from the nasal and tracheal swabs showed that the pigs sired by one boar (B3) had a distinctive colonization pattern, different from that of the pigs from the other 2 boars and from the positive controls. SEM studies demonstrated that at 35 d postinoculation a higher proportion of B3 pigs had lower numbers of mycoplasmas attached to the cilia compared with B1 and B2 offspring. No significant differences were observed in temperature and weight gain among groups by ANOVA; however, with use of a 2 × 2 table, temperature differences were observed between pigs sired by boars B1 and B2 at 4 d postinoculation. No pigs seroconverted, showed gross or microscopic lesions, or had positive IFAT results. These results provide evidence of differences in patterns of colonization between pigs sired by different boars, suggesting a possible genetic effect. 相似文献
83.
江苏省盐城市所辖的射阳县洋马乡和东台市三仓镇,近几年来,在农业结构调整中,因地制宜确立主导产业,千方百计培育主导产业,分别成为远近闻名的中药材之乡和西瓜之乡,1999年洋马乡中药材种植面积达3333.3公顷,实现产值1.6亿元,分别占全乡耕地面积和种植业产值的70%和75%;三仓镇种植大棚西瓜2020公顷,实现产值1.2亿元,分别占全镇耕地面积和种植业产值的40%和64.6%. 相似文献
84.
对24例成黄牛的前肢脚部动脉采用解剖,铸型和动脉造影等方法进行研究。结果:1。中国黄牛前脚部掌侧动脉主干为正中动脉,指掌侧第Ⅱ总动脉,在近指节骨远端指掌侧第Ⅲ总动脉分为第Ⅲ,Ⅳ指掌侧固有轴动脉,经远指节骨轴孔入骨质管。2.指掌侧第Ⅲ总动脉在掌骨远端分出拽第Ⅱ总动脉,在近指节骨远端指掌侧第Ⅲ总动脉,分出背侧支和掌侧支,后者分别与指掌侧第Ⅱ,Ⅳ总动脉吻合形居近指节深弓,在掌侧支稍下方,指掌侧第2,Ⅳ指 相似文献
85.
利用犬温热,细小病毒性肠炎,犬传染性肝炎,狂犬病等弱毒株,以香菇多糖和动物的核糖,多肽做为免疫增强剂,对异源动物羊,猪,犊牛,家兔进行了免疫应答,筛选出了猪做为异源免疫动物。在免疫增强剂的参与下,完成了多次免疫应答,达到了理想的免疫球蛋白(IgG),通过试验摸清了该制剂的免疫程序,确定了生产工艺,经重复性试验,稳定性试验及含量测定,以及临床应用试验和现志应用试验均达以了预期效果,该制剂经冷冻干燥后 相似文献
86.
1959年,将初选出的35个优良家系作进一步的区域性试验,经过4a观测研究后认为.①61号等8个家系,表现突出,在我市推广,增产明显,②选出的17株优树,同初级良种相比,第4年增产138.2%,成熟期可望增产37.7%,③杉木4a生时,进行选择是较为可靠的. 相似文献
87.
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89.
The effect of exploratory laparotomy on the serum and peritoneal haptoglobin concentrations of the pony. 总被引:1,自引:0,他引:1
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Serum haptoglobin concentration was used as an indicator of the acute phase response in ponies undergoing exploratory laparotomy. Preoperative, 1 h intraoperative, 3 h, 6 h, 12 h and 24 h postoperative blood samples and 48 h postoperative peritoneal fluid samples were obtained for haptoglobin analysis. A spectrophotometric assay based on cyanmethemoglobin binding capacity (CyanBC) was used to determine haptoglobin concentrations. The preoperative reference range for serum haptoglobin concentrations in these ponies was 25-60 mg CyanBC/dL. Intraoperative and 3 h postoperative blood samples had decreased haptoglobin concentrations when compared to preoperative values. Serum haptoglobin concentrations began to rise by the 6 h postoperative sample and were generally elevated above preoperative values by the 24 h postoperative sample. Two of the ten ponies had mild signs of postoperative colic which were associated with twofold elevations in serum haptoglobin concentrations and fivefold elevations in peritoneal fluid haptoglobin concentrations. 相似文献
90.
J Tetens S A Barker M Waguespack G Hosgood 《Canadian journal of veterinary research》2001,65(2):133-135
The objectives were to use high performance liquid chromatography (HPLC) to validate an established method for adenine nucleotide separation in equine colonic mucosal tissue, to determine the inherent variability in the tissue and extraction method, and to determine the stability of ATP, ADP, and AMP in the tissue with time. Equine colonic mucosal tissue obtained from a single horse was immediately submersed in liquid nitrogen, and stored at -70 degrees C. Samples were lyophilized, extracted, and separated by HPLC. The limit of quantitation was 0.05 microg/mL. The coefficient of variation for the instrument was less than 10% for all nucleotides measured. When the tissue was not homogenized prior to sampling, there were significant differences in adenine nucleotide content between samples. However, when the tissue was homogenized prior to analysis, these differences were no longer significant. There was no significant decrease in ATP, ADP, or AMP content over a 54-day analysis period. 相似文献