水稻种子总RNA提取质量问题的探究

水稻种子总RNA提取在水稻分子遗传基础实验中起着非常重要的作用,然而,高质量水稻种子RNA的获取难度高,极大阻碍了cDNA文库构建、RNA-Seq和基因表达分析等相关研究工作的开展和深入。为了探讨水稻种子总RNA提取的相关影响因素,寻找合适的水稻种子总RNA提取方式,本研究通过比较Trizol法、GeneMark试剂盒法和艾德莱试剂盒法3种不同的提取方式、不同的耗材处理方法、研磨方式等不同因素改进水稻种子总RNA的提取效果。不同的检测结果显示,艾德莱多糖多酚提取试剂盒能够提取到28S、18S条带完整、降解少和纯度高的水稻种子总RNA;不同耗材处理方法中,蛋白酶K处理过的耗材提取的总RNA比另外两种方法处理过的耗材提取的总RNA降解更少,28S和18S条带更清晰和完整、总RNA质量最高、效果最好;而研磨方式上,液氮冷冻研磨法能够有效抑制RNA酶对RNA的降解。本研究对水稻种子RNA提取过程相关因素的分析和改进,为促进以水稻种子为研究对象的分子实验奠定了一定的技术基础。     

早杂新组合T78优2155综合栽培技术措施初探

通过对T78优2155早季栽培施肥水平、施用方法、密植规格的统计分析,结果表明以每667m2施纯N14kg、插植规格20 cm×20 cm,基肥、蘖肥、穗肥比例为5:3:2效果最佳,有利于获得高产。     

Uterine Expression of WNT7A and β-catenin After Induction of Oestrus in Sheep

WNT7A and β-catenin localisations and roles in regulating periimplantation ovine conceptus development under natural estrous conditions have been elaborated.However,their locations and expression patterns have not been reported under induction of oestrus.The localisation,expression and function of WNT7A and β-catenin in the uterine tissues of the early pregnant and non-pregnant sheep on days 10,12,14,16 and 18 following artificial induction of oestrus were investigated by means of in situ hybridisation,real-time RT-PCR,immuno-histochemistry and western blotting methods.WNT7A and β-catenin mRNA and protein were both restricted to the apical surfaces of the uterine luminal epithelium (LE) and glandular epithelium (GE).In pregnant sheep,protein localisation of WNT7A and β-catenin was observed both in the endometrial LE and GE.Their staining presented on day 10,increased between day 12 and day 16,and decreased on day 18.WNT7A and β-catenin mRNA and protein expression increased initially and then decreased from day 10 to day 18,peaking on day 16,and β-catenin reaching a peak on day 18 in the uterine tissues of pregnant sheep (p0.05).By contrast,no significant changes in WNT7A and β-catenin mRNA and protein expression levels were observed from day 10 to day 18 of the oestrus cycle in the uterine tissues of non-pregnant sheep (p0.05).Additionally,WNT7A and β-catenin mRNA and protein expression levels in the uterine tissues of the early pregnant sheep were significantly higher than those of non-pregnant sheep (p0.05).Treatment of endometrial epithelial cells with WNT7A increased the mRNA expressions of β-catenin,c-myc and Cyclin D1.These results provided an underlying mechanism of periimplantation ovine conceptus development under induction of oestrus.     

晚春造林技术研究

通过简单易行而有效的技术措施,成功试验且规模推广晚春造林技术,解决了当地春季火烧迹地更新造林季节脱节的矛盾。     

水丰水库渔获变化和鱼类资源现状及小型经济鱼虾种间关系分析

水丰水库的渔获量1961－1980年间变化于47－300t,1981-1984年增长到740－1321t,1985-2000年为2270－4926t。渔获物组成中以亚洲公鱼为主，1980－2000年亚洲蚣鱼占总渔获的29．6％－93．0％，平均为65．6％。鲁鲫鱼占6．2％－16．4％，鲢鳙鱼占8．1％－13．3％，鳊鲂占1．8％－11．3％，虾占5．9％－16．1％，其它鱼类占2．4％－9．3％。2000年大银鱼占总渔获的2．8％。1985－2000年水库鱼类资源相对稳定。亚洲公鱼、青虾和大银鱼三种小型经济鱼虾的种间关系表现为互利共生，竞争和残敌关系，可能向种间形成平衡调节方向发展。     

Effect of Chitosan on Intestinal Bacteria of Allogynogenetic Crucian Carp,Carassius auratus gibelio,as Depicted by polymerase chain reaction‐denaturing Gradient Gel Electrophoresis

A 16S rDNA‐based polymerase chain reaction‐denaturing gradient gel electrophoresis (PCR‐DGGE) method was applied to detect intestinal bacterial communities of juvenile allogynogenetic crucian carp, Carassius auratus gibelio, fed with chitosan‐containing diet. This is the first time to use the molecular method to analyze the bacterial communities in the allogynogenetic crucian carp intestine. The DGGE profile with universal bacterial primers revealed simple communities in all treatment groups. Sequencing and phylogenetic analysis of excised DGGE bands showed that the dominant bacteria belonged to class γ‐Proteobacteria and Fusobacteria. The relative abundance and diversity of detected bacteria suggested that 0.5 and 0.75% of chitosan in diet were optimum for juvenile allogynogenetic crucian carp. As in these concentrations, some detected pathogen bacteria either disappeared or decreased. However, the DGGE profile with Aeromonas‐specific primers showed a similar composition among all treatment groups, which suggested that Aeromonas was one of relative stable bacteria components in the intestine of juvenile allogynogenetic crucian carp.     

关于公鱼属鱼类分类的讨论

本文回顾了公鱼属鱼类的分类历史,讨论了分类存在的问题,提出了赞同把该属分成形态特征和生态习性明显不同的两个种的意见:淡水产卵的定为池沼公鱼(H.olidus(Pallas),海水产卵的定为海公鱼(H.pretiosus(Girard)。     

江黄颡鱼人工繁育的初步研究

对江黄颡鱼人工繁殖和育苗进行了试验。以PG(鲤鱼脑垂体激素)、HCG(绒毛膜促性腺激素)等催产药物进行混合催产,水温24～26℃,效应时间为20～24h,产卵率为90%以上。采用等渗液稀释磨碎的精巢进行人工授精,用塑料网片着卵板粘卵,在孵化池内孵化,平均孵化率为87%。研究结果表明:江黄颡鱼在广西的适宜催产季节为4月中旬至6月底,工厂化育苗的饵料可用丰年虫和适口虾料来解决。     

干燥过程的变质量热力学分析

对一种典型热泵干燥系统进行了实验研究,并进行了干燥过程的变质量热力学分析,其方程具有普遍性。对过程进行质量守恒分析和变质量热力学第一定律分析,得到了干燥过程需热量和干燥效率等参数,并与经典热力学分析方法做出比较,最后给出了实际干燥过程为等焓过程的条件。     

一种基于PERCLOS驾驶员疲劳检测方法的实现算法

PERCLOS法能方便的对驾驶员进行实时、非接触式疲劳检测,在肤色检测的基础上结合眼睛的形状并利用梯度信息定位眼睛,通过计算眼睛的面积变化来确定眼睛的睁开、闭合状态,记录眼睛睁开闭合的次数及其开始、结束时刻,计算PEKCLOS值,当PERCLOS值大于40%,眼睛持续闭合时间大于3 S时,就认为驾驶员属于疲劳驾驶.