羽叶决明代木栽培鸡腿菇的物质转化

研究羽叶决明(Chamaecrasta nictitans)代木栽培鸡腿菇(Coprinus ovatus)对其绝对生物学效率、基物失重、呼吸消耗及木质纤维素转化的影响.结果表明,羽叶决明牧草替代60%木屑(A60)栽培鸡腿菇的绝对生物学效率最高,均为10.77%.覆土前和采收后的基物失重和呼吸消耗都以A0(CK)为最高,分别为11.47%、11.47%和31.75%、25.55%,其木质素转化率也最高,为79.21%;纤维素转化率以A100(羽叶决明牧草替代100%木屑)为最高,达50.93%;半纤维素转化率以A40(羽叶决明牧草替代40%木屑)为最高,为49.74%.回归分析结果表明,绝对生物学效率和半纤维素转化率与替代比例呈抛物线相关,而基物失重、呼吸消耗及木质素、纤维素转化率与替代比例呈线性相关.     

整地施肥对尾叶桉生长效应的研究

整地施肥试验结果表明，全垦，带垦和穴垦３种整地方式及施肥对尾叶桉造林成活率和保存率均没有显的影响，这３种整地处理加上施肥，对促进尾叶桉生长均能取得理想的效果，５年生材积，施肥是不施肥的９．８倍，全垦和带垦整地虽然在一定程度上促进了林木的生长，但在贫瘠的立地上，加施肥料才能达到速丰产经营目的，整地和施肥间的交互作用不明显，施肥对尾叶桉的促进作用可延至５年以后。     

刚果12号桉施肥效应研究

在贫瘠低丘上对刚果１２号桉进行不同肥料配合和不同用量的施肥试验，结果表明ＮＰＫ，ＮＫ，ＮＰ和ＰＫ配合施用的能极显著地促进刚果１２号桉的生长，５年生时蓄积比对照分别增加１９．４０，１８．４５，１６．４０，１５．０５ｍ＾３／ｈｍ＾２，Ｎ，Ｐ，Ｋ肥单施的蓄积比对照纷别增加５．３０，５．２５，４．８０ｍ＾３／ｈｍ＾２肥料的配合施用极显著地优于Ｎ，Ｐ，Ｋ单施，材积的增长量随施肥量的增加而增加。     

浙江省银杏生产的现状和对策

分析了浙江省银杏资源及其开发利用现状的基础上，揭示了发展银杏的优势和有利条件，以及银杏生产的存在问题，提出了发展银杏生产的对策和措施。     

岷江上游干旱河谷辐射松种子育苗试验研究

经过连续 3年对辐射松种苗特性及育苗技术的试验研究 ,结果表明 :( 1)辐射松种子在 5℃的低温条件下贮藏 2年其发芽率比对照提高 6 4 5 % ;( 2 )用 0 5 %的高锰酸钾液对种子进行消毒处理其幼苗出苗率提高 39 2 % ;( 3)用湿润河砂层积催芽的种子其苗木平均苗高 2 4 0cm ,平均地径 0 33cm ,比对照苗高多 4 8cm ,地径大 0 12cm ;( 4)酸性土上培育的苗木平均苗高比碱性土上培育的苗木平均苗高多 9 8cm ,平均地径大 0 16cm ;( 5 )营养袋规格 10cm× 15cm ,营养土配方为腐殖含量 5 0 %的平均苗高 2 3 3cm ,平均地径 0 3cm ,达到一级苗标准 ;( 6 ) 2月底 3月初播种的苗木比 4月底 5月初播种的苗木苗高多 9 1cm ,平均地径大0 16cm ,出苗率提高 35 6 %。经过综合试验研究 ,场圃出苗率由 40 %提高到 80 %以上 ,每公顷产一年生合格营养袋苗达 12 0万株。     

Seasonal variations in photosynthesis of Picea morrisonicola growing in the subalpine region of subtropical Taiwan

From January 1999 to May 2001, we investigated seasonal variations in the photosynthetic capacity of Taiwan spruce (Picea morrisonicola Hay.) growing in the subalpine region of subtropical Taiwan (23 degrees 29' N, 120 degrees 53' E, 2600 m a.s.l.). Photosynthetic capacity (near light-saturated net photosynthetic rate, Pnsat, chlorophyll fluorescence (Fv/Fm) and soluble protein concentration of needles all increased from mid or late spring to early winter. Even when minimum air temperature of the measuring day dropped to near 0 degrees C, Pnsat remained at about 20% of the highest value observed in winter. There was a curvilinear relationship between Fv/Fm and the minimum or mean air temperature of the measuring day. The increase in Fv/Fm with temperature was slowed when the daily mean air temperature was above 7 degrees C, or the minimum air temperature was above 3 degrees C; however, when air temperatures dropped below these values, Fv/Fm varied sharply. Seasonal variations in Pnsat paralleled those in Fv/Fm and needle soluble protein concentration. In early or mid spring when air temperature and Fv/Fm increased, Pnsat and soluble protein concentration remained low. Multiple regression analysis showed that seasonal variations in Pnsat were affected by Fv/Fm, air temperature and needle soluble protein concentration, and the multiple regression equation could be used to estimate Pnsat in different seasons. We conclude that the decrease in photosynthetic capacity of Taiwan spruce in winter and its subsequent recovery in spring were mainly caused by photoinhibition and its reversal, and changes in needle soluble protein concentration. Another possible explanation for the delayed recovery of photosynthetic capacity in spring may be associated with the slow increase in needle soluble protein concentration.     

修枝间伐对松突圆蚧抑制作用的研究

严重危害广东省沿海地区马尾松的松突圆蚧(Hemiberlesia pitysophila Takagi),自1982年在深圳、珠海等地发现后，至今危害面积约43万ha，其中枯萎或涉死的约8万ha。     

Genetic characterization of bovine viral diarrhea virus strains in Beijing,China and innate immune responses of peripheral blood mononuclear cells in persistently infected dairy cattle

To acquire epidemiological data on the bovine viral diarrhea virus (BVDV) and identify cattle persistently infected (PI) with this virus, 4,327 samples from Holstein dairy cows were screened over a four-year period in Beijing, China. Eighteen BVD viruses were isolated, 12 from PI cattle. Based on genetic analysis of their 5''-untranslated region (5''-UTR), the 18 isolates were assigned to subgenotype BVDV-1m, 1a, 1d, 1q, and 1b. To investigate the innate immune responses in the peripheral-blood mononuclear cells of PI cattle, the expression of Toll-like receptors (TLRs), RIG-I-like receptors, interferon-α (IFN-α), IFN-β, myxovirus (influenza virus) resistance 1 (MX1), and interferon stimulatory gene 15 (ISG15) was assessed by qPCR. When compared with healthy cattle, the expression of TLR-7, IFN-α, and IFN-β mRNA was downregulated, but the expression of MX1 and ISG-15 mRNA was upregulated in PI cattle. Immunoblotting analysis revealed that the expression of interferon regulatory factor 3 (IRF-3) and IRF-7 was lower in PI cattle than in healthy cattle. Thus, BVDV-1m and 1a are the predominant subgenotypes in the Beijing region, and the strains are highly divergent. Our findings also suggest that the TLR-7/IRF-7 signaling pathway plays a role in evasion of host restriction by BVDV.