利用游离小孢子培养育成早熟大白菜新品种‘豫新5号’

 ‘豫新5号’是采用游离小孢子培养双单倍体育种技术选育的一代杂种，该品种生育期60 d，单球质量23 kg，球形指数1.35，高抗病毒病、霜霉病和软腐病，净菜产量56.7 t·hm^-2，可兼作早熟、晚熟栽培。     

Sca-1+ cells from mouse fetal liver can differentiate into neurons in vitro

AIM: To study whether Sca-1⁺ cells from fetal liver can be induced to differentiate into neuronal cells in vitro. METHODS:Sca-1⁺cells from 14 5-days-old murine fetal liver were isolated with a magnetic cell sorting kit, and were cultured in Dulbecco s modif ied Eagle s medium(DMEM)/F12 supplemented with 10%fetal bovine serum(FBS), and passaged at a rat io of 1 3 when cells reached more than 80%confluence.The 5 passage cells were induced by 10^-3mol/Lβ-mercaptoethanol(β-ME)and 5×10^-7 mol/L all-trans-retinoic acid(RA)for 24 hours, and then incubated in serum-free medium for 5 hours to 5 days.The characteristics of treated cel s were assayed by immunocytochemistry staining analysis at 5 hours, or 5 days.RESULTS: Cells treated with β-ME and RA exhibited neuronal phenotype and expressed neuron-specific protein such as neuron-specific nuclear protein (NeuN), neuronfilament-M, and neuron-specific tubulin-1 (TuJ-1) but not tau, MAP-2, or the astrocyte-specific marker glial fibrillary acidic protein (GFAP).CONCLUSION: Sca-1⁺ cells from fetal liver, of which most are regarded as hematopoietic stem cells, could differentiate into early immature neuronal cells in vitro. These findings suggest that Sca-1⁺ cells from fetal liver may be an alternative source in cell therapy and gene therapy of neural dysfunction.     

The transdifferentiation of Sca-1+ cells from murine fetal liver

AIM:To explore transdifferentiation potential of Sca-1⁺ cells from murine fetal liver. METHODS:2×10³ of Sca-1⁺ cells from male murine fetal liver were transfused into female mouse irradiated lethally with γ ray from ⁶⁰ Co source (10 Gy) via tail vein. Two months later, FISH and immunohistochemistry were used to detect the situation for transdifferentiating of the donor cells (male cells) in tissues of female recipient mouse. RESULTS:The renal tubular epitheliocyte-like and neurocyte-like cells with Y chromosome were found on the sections of renal and brain tissues from female recipient mice. These cells have phenotype characteristics of RCA⁺/CD₄₅^-F_4/80^- and NueN⁺/CD₄₅^-F_4/80^-, respectively. CONCLUSION:The evidence is provided for Sca-1⁺ cells from murine fetal liver to transdifferentiate into both renal and brain tissue cells.     

胶体金免疫层析法快速检测烟草环斑病毒

采用柠檬酸三钠还原法制备胶体金颗粒 ,标记烟草环斑病毒的抗体 ,制成免疫层析检测试纸条。检测粗提纯病毒的灵敏度为 1 0 0 0ng/ml,病汁液稀释 1 0 0 0倍后仍可快速检出。对大豆病种子、烟草冻干病叶等不同材料进行检测也有良好的效果 ,1～ 2min即可出现结果。用 9种不同的病毒进行测试 ,未出现非特异性反应。     

植物杀虫剂苦皮藤素对柑橘潜叶蛾控制效果的研究

用植物性杀虫剂苦皮藤素Ⅳ(KPT乳油)有效成分30、15mg/kg防治柑橘潜叶蛾,采用离体叶片浸叶法处理5s,3d后,幼虫校正虫口减退率分别为89.63%、63.02%;枝梢浸液法处理5s,5d后,潜道伸长度校正抑制率分别为76.12%和41.51%;10d后,对秋梢的相对保叶效果分别为69.34%和52.29%。结果表明,采用KPT乳油有效成分30mg/kg对柑橘潜叶蛾有较好的控制作用,对柑橘秋梢有良好的保护效果。     

不同抗性的大豆品种感染细菌性疫病后POD、PPOD的研究

 大豆细菌性瑾病(Pseudomonas syringae pv.glycinea简称PSG)是我国和世界大豆产区的主要病害在适宜的发病条件下可使大豆减产18%~22%,最高可达到29%以上。     

根癌农杆菌介导绿色荧光蛋白基因转化印度酸桔的研究

 通过根癌农杆菌介导将绿色荧光蛋白基因转入印度酸桔的胚性愈伤组织中, 经潮霉素筛选,获得抗性愈伤组织, 并再生植株。对这些植株进行GUS 染色、PCR 分析、绿色荧光检测和Sourthern 杂交验证, 结果表明绿色荧光蛋白已经在转基因植株中表达。     

‘新红星’苹果果实蔗糖合酶的活性及亚细胞定位

 在‘新红星’苹果果实发育过程中, 伴随果糖、葡萄糖和蔗糖的积累, 蔗糖合酶的分解活性逐渐下降, 而合成活性逐渐升高。苹果果实的蔗糖合酶由分子量为90 kD 的亚基组成, 其免疫信号强度随发育进程而增加。蔗糖合酶主要定位于果肉细胞的细胞质中, 发育中后期该酶的分布密度有一定增加。综合结果认为, 蔗糖合酶调控发育早期蔗糖的降解和发育后期蔗糖的积累。     

苹果低氧气调新组合贮后效应的利用研究

利用0.12mm聚乙烯薄膜大帐进行低氧气调新组合的较大规模应用试验,对2394kg玫瑰红苹果进行低氧处理,帐内O2浓度可下调至约2%,CO2浓度可自动维持在5%～8%的较理想状态,处理结束后很易转为MA自发气调。低氧处理后再MA贮藏的苹果,虎皮病果率低、症状轻,腐烂果少,果柄和果皮新鲜,叶绿素保持效果好,果肉硬度明显高于小包装气调和冷藏对照。说明利用塑膜大帐进行前期低氧新组合处理与后期自发气调、实现低氧气调新组合贮后效应的利用是可行的。