Molecular cloning and functional analysis of goose interferon gamma

The cDNA for goose interferon gamma (goIFN-gamma) was cloned from PHA-stimulated goose peripheral blood mononuclear cells (PBMCs) by RT-PCR. This cDNA encodes a 19-amino acid signal peptide and a 145-amino acid mature protein, which shares a high homology with duck IFN-gamma. Recombinant mature goose interferon gamma (rgoIFN-gamma) generated from both prokaryotic and eukaryotic expression systems effectively inhibited the replication of goose paramyxovirus and recombinant vesicular stomatitis virus in vitro. These antiviral activities were abrogated by rabbit anti-rgoIFN-gamma antibodies in vitro. Furthermore, rgoIFN-gamma stimulated goose peritoneal macrophages to produce nitric oxide (NO) in vitro, demonstrating its macrophage activating factor (MAF) activity. Therefore, the availability of bioactive rgoIFN-gamma and its specific antibodies provides valuable tools for studying T cell immunity in geese.     

宠物犬比美貌工作犬展雄姿——记者眼中的"2007南京全犬种展评暨训练比赛"

在这里可以学习各类犬展赛事的专业知识,如牵犬秘诀、赛前美容、犬展赛制等等,同时也能找寻到你心目中喜爱的犬的身影。     

洛伐他汀(Lovastatin)对鸡蛋和鸡血浆中胆固醇含量的影响

将44只新扬州鸡随机平均分成4组.对照组用基础日粮,试验组在饲喂基础日粮的同时分别添加0.015%、0.03%和0.06%的洛伐他汀(Lovastatin),研究洛伐他汀对鸡蛋和血浆中胆固醇含量的影响,试验期为5周.试验结果表明:洛伐他汀能显著降低产蛋鸡血浆和鸡蛋中胆固醇水平(P＜0.05),但对产蛋率的影响不显著(P＞0.05).     

城市巡逻犬的训练

一、巡逻犬的挑选(一)品种。作为城市巡逻犬,主要是要具备对犯罪分子的威慑力,因此,大型犬是首选的目标。目前,主要有德国牧羊犬、昆明犬、罗威纳犬和马里努阿犬,以及上述犬种的杂交犬。(二)年龄。0.6～2岁为最佳训练年龄。(三)体型外貌。所选择受训犬的体型外貌必须是:外观各部匀称,体格强壮,毛色光亮,肌肉发达,牙齿整齐并结实有力,嗅觉灵敏,视觉和听觉良好,还要注意犬的自身协调性,灵活性,奔跑速度以及应激反应等。(四)神经类型。最好选择属于兴奋型和活泼型的两大类犬。(五)主要反应。受训犬必须是猎取反射积极,主动防御反应强烈,食物反…     

蒽酮-硫酸比色法测定天然鸽乳中可利用糖含量的研究

采用蒽酮-硫酸比色法测定玉米中的可利用糖含量以确定试验测定的最佳条件,并以此条件为对照测定天然鸽乳中可利用糖含量.玉米及天然鸽乳可利用糖溶液经乙醚去脂,高峰氏糖化酶水解,乙醇提取,饱和乙酸铅脱色后制得.结果表明,葡萄糖标准溶液的测定量在0.1～0.6 mg/mL的浓度范围内有良好的线性关系,其回归方程为y=1.6771x 0.0737,相关系数R=0.9987.测定不同的天然鸽乳样品,试验精密度高,重复性好,其可利用糖含量,0～3日龄天然鸽乳为7.45%～7.64%,4～6日龄天然鸽乳为32.08～60.28 %.     

堵源截流的先锋贩毒分子的克星——前进中的临沧警犬缉毒工作

在滇西南边陲临沧290.79公里的中缅边境线上,常年活跃着一支能征善战的缉毒专业队伍——临沧市公安局禁毒支队缉毒警犬防暴大队。他们在长期的禁毒斗争中取得了令人瞩目的成绩,以每年侦破数起万克以上特大案件的辉煌战绩,成为临沧边疆堵源截流的先锋,贩毒分子的克星,为减少毒品的内流,减轻毒品对社会的危害做出了突出的贡献。2000年,刑侦犬“双刮”代表云南省参加在南京举行的第一届全国警犬技术大赛,     

绍鸭光过敏

１９９７年８月２日笔者曾在兽医门诊接触１例绍鸭光过敏,现报告如下。１　发病情况　我县沿河乡芦南村村民赵××于１９９７年４月初自浙江绍兴引进１日龄绍鸭１万余只,赵留下６００只,于天然河荡处放牧,其余分给他人于大河畔圈养。自４日龄起,赵发现整个鸭群精神萎顿,采食减少,并见近４００只幼鸭的上喙出现红斑,１～２天后,红斑逐渐变黑,且上喙变得异常柔软,触之易出血。病鸭采食困难,食量骤减,增重缓慢。该畜主曾用青霉素、土霉素、复方新诺明治疗,均无明显效果。将１５只弱雏隔离舍饲,发现症状减轻。大群中,发病严重的…     

Virus antigen expression and alterations in peripheral blood mononuclear cell subpopulations after classical swine fever virus infection.

Depletion in the number of lymphocytes and viral persistence are thought to be the most important outcomes of classical swine fever virus (CSFV) infection. To define the change in peripheral blood mononuclear cells (PBMC) and virus replication in leukocytes after CSFV infection, 8-week old pigs were infected with the LPC vaccine strain or virulent CSFV (HCV-YL strain). Changes in the relative number of PBMCs were analyzed by flow cytometry. The results showed a significant increase in the relative percentage of monocytes in PBMCs during acute CSFV infection of naive pigs (p < 0.05). Monocyte frequencies were not changed in LPC-vaccinated pigs and control pigs. There was also a significant decrease in the number of IgM+ cells (p < 0.05) and a slight decrease in the number of CD4+ lymphocytes after 5 days of infection. There was no change in the frequency of CD8+ lymphocytes in PBMCs after infection. To define which subpopulation of PBMCs was the target for CSFV infection, PBMC populations from CSFV infected pigs were separated and stained for virus antigen expression. Alveolar macrophages (AM) were also studied. The results showed that CSFV replicated in all PBMC subpopulations: CD4+, CD8+, and IgM+ lymphocytes, and monocytes as well as AMs. However, virus antigen expression was more intense in monocytes and AMs. The infection of lymphocytes may, therefore, contribute to the depletion in their numbers after infection and lead to defective antibody production during virulent CSFV infection.     

冷温血停搏液心肌保护效应血清CK—MB,CTn—T的变化研究

为探讨血停搏液不同灌注对心肌保护效应,２８只低温体外循环犬,按停搏液灌注方式随机分为四组：Ａ组为冷晶组,Ｂ组为冷血组,Ｃ组为温血组,Ｄ组为冷温血组。