全文获取类型
收费全文 | 50538篇 |
免费 | 3220篇 |
国内免费 | 4384篇 |
专业分类
林业 | 3942篇 |
农学 | 2346篇 |
基础科学 | 2286篇 |
4748篇 | |
综合类 | 25123篇 |
农作物 | 3815篇 |
水产渔业 | 2219篇 |
畜牧兽医 | 7696篇 |
园艺 | 3709篇 |
植物保护 | 2258篇 |
出版年
2024年 | 419篇 |
2023年 | 1093篇 |
2022年 | 2385篇 |
2021年 | 2438篇 |
2020年 | 2232篇 |
2019年 | 2170篇 |
2018年 | 1490篇 |
2017年 | 2441篇 |
2016年 | 1533篇 |
2015年 | 2419篇 |
2014年 | 2605篇 |
2013年 | 3168篇 |
2012年 | 4274篇 |
2011年 | 4362篇 |
2010年 | 4060篇 |
2009年 | 3663篇 |
2008年 | 3588篇 |
2007年 | 3364篇 |
2006年 | 2814篇 |
2005年 | 2111篇 |
2004年 | 1482篇 |
2003年 | 872篇 |
2002年 | 897篇 |
2001年 | 923篇 |
2000年 | 808篇 |
1999年 | 281篇 |
1998年 | 39篇 |
1997年 | 16篇 |
1996年 | 17篇 |
1995年 | 16篇 |
1994年 | 14篇 |
1993年 | 13篇 |
1992年 | 15篇 |
1991年 | 15篇 |
1990年 | 9篇 |
1989年 | 4篇 |
1988年 | 5篇 |
1987年 | 8篇 |
1986年 | 9篇 |
1985年 | 2篇 |
1981年 | 11篇 |
1965年 | 2篇 |
1964年 | 2篇 |
1962年 | 15篇 |
1961年 | 1篇 |
1958年 | 2篇 |
1957年 | 1篇 |
1956年 | 24篇 |
1955年 | 7篇 |
1954年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
111.
112.
鸭大肠杆菌的药物敏感性试验 总被引:3,自引:0,他引:3
采用纸片扩散法测定了14株鸭大肠杆菌对10种抗菌药物的敏感性,结果鸭大肠杆菌对头孢唑啉、头孢噻肟、丁胺卡那霉素最为敏感,敏感率均为100%;其次为庆大霉素,敏感率为78.6%;对其他抗菌药物均产生不同程度的耐药性,耐药性最为严重的是四环素、复方新诺明及多西环素,耐药率分别为92.9%、85.7%、78.6%,对阿莫西林、氯霉素、环丙沙星的耐药率分别为57.1%、50.0%、64.3%;78.6%的菌株对10种抗菌药物表现为多重耐药性.耐阿莫西林菌株经碘-淀粉测定法测定均为产β-内酰胺酶菌株,阿莫西林对它们的最小抑菌浓度范围为256~ 1 024 mg/L. 相似文献
113.
亚热带牧用草地中威提特东非狼尾草的种子繁殖研究 总被引:4,自引:0,他引:4
在3月和4月对全日制放牧的东非狼尾草草地上的风干牛粪取样,进行发芽试验,分别测定其中的东非狼尾草发芽种子数量。结果,3月取样平均432.5 g的牛粪中东非狼尾草平均发芽种子数量为118.25粒,4月取样平均437.8 g的牛粪中东非狼尾草平均发芽种子数量为52.8粒,二者平均牛粪重为435.15 g,平均发芽种子为85.5粒,说明牛粪中东非狼尾草种子数量较多,是其繁殖扩展较快的主要原因;3月取样的种子数量为4月取样数量的1.38倍,说明人工利用牛粪进行东非狼尾草繁殖时,需在有降雨来临前准备,即1~3月进行牛粪的准备工作。 相似文献
114.
谷氨酰胺对肉仔鸡脾组织结构发育的影响 总被引:2,自引:1,他引:2
160只 1日龄艾维茵肉仔鸡随机分成 4组 ,分别饲喂添加 0 % ,0 2 % ,0 4%和 0 8%谷氨酰胺的饲粮 2 8d。每周末每组取 6只鸡 ,颈静脉放血致死 ,取脾脏 ,Bouin液固定 ,制作石蜡切片 ,HE染色 ,光镜观察 ,显微摄影 ,研究基础日粮中添加谷氨酰胺对肉仔鸡脾组织结构的影响。结果显示 :添加谷氨酰胺的各试验组脾小结增多、增大 ;动脉周围淋巴鞘增厚 ;椭球增多、增大 ,细胞排列疏松 ,鞘毛细血管内皮细胞增高、增多 ;红髓比例降低 ,脾索内浆细胞和巨噬细胞增多。添加 0 8%谷氨酰胺的脾小结变化明显 ,添加 0 4%谷氨酰胺的动脉周围淋巴鞘和椭球变化明显。说明日粮中适量添加谷氨酰胺可促进脾内淋巴细胞的增殖与分化 ,从组织学角度证明 ,谷氨酰胺可促进脾的免疫应答能力 ,提高机体的免疫功能 相似文献
115.
猪大肠杆菌水肿毒素SLT-IIeA基因的克隆和序列分析 总被引:2,自引:0,他引:2
研究以本地猪水肿病大肠杆分离物ED1株为材料 ,利用 PCR克隆了含猪水肿病大肠杆菌 (VTEC) slt-IIe A基因 987bp的片段 ,并测定了含该克隆片段的 Bam HI、Hind 酶切片段的核苷酸序列。结果表明 ,slt-IIe A基因的编码区全长 960 bp,编码 3 1 9个氨基酸的蛋白质 ,序列与国外报导的 S1 1 79株进行比较发现其核苷酸同源性为 98.9%。经推导的氨基酸序列的同源性为 99.7%。这为进一步研究 slt-IIe A的生物学特性、水肿病的分子诊断及其防制打下基础 相似文献
116.
丹参(Salvia miltorrhiza Bge.)为唇形科鼠尾草属草本植物,以根入药,是我国一种常用大宗中草药。近年来河北安国中药材基地丹参上一种退化病十分严重,该病田间发病率高,有的田块高达60%~80%,表现为花叶、斑驳、卷叶、黄化、矮化等症状,严重影响了丹参的产量和品质。我们利用生物学、电镜观察、血清学方法和基因序列测定等方法对病原进行了初步研究,发现黄瓜花叶病毒(Cucumber mosaic virus,CMV)与该病害密切相关 相似文献
117.
AIM: To study the role of liver in immune regulation in experimental endotoxemia. METHODS: 17 castrated male goats were subjected to simultaneously installing catheters in jugular, hepatic and portal veins by surgery. Four days later, lipopolysaccharide (LPS) was infused in term of three groups as followings: In group ①, LPS of 20 EU (endotoxin unit, EU)·kg-1 was infused into portal vein; In group ②, LPS of 20 EU·kg-1 was infused into jugular vein and LPS of 1 500 EU·kg-1 infused into jugular vein in group ③. Before and after infusion, blood samples were collected from the three veins through the catheters for 8 h.The plasma levels of TNF-α were measured by RIA. RESULTS: In group ①, the plasma TNF-α levels of hepatic and portal vein rose to peak value at 5 h, but that of the jugular vein did not changed. In group ②, the plasma TNF-α levels in hepatic vein rose to peak value at 3 h. The TNF-α levels of jugular vein rose to peak value at 1 h and the one in portal vein enhanced continuously between 0-8 h. In group ③, the plasma TNF-α levels in jugular, hepatic and portal vein rose to significant peaks at 1 h simultaneously. CONCLUSION: During experimental endotoxemia,liver showed different dynamic characteristics in TNF-α secretion according to the pathway and doses of LPS delivery. 相似文献
118.
DING Gui-xia ZHANG Ai-hua HUANG Song-ming WU Yuan-jun FEI Li GUO Mei CHEN Rong-hua 《园艺学报》2004,20(10):1754-1758
AIM: To investigate the role of NF-κB/IκB signal pathway in the regulation of cyclooxygenase-2 (COX-2) expression in human mesangial cells (HMC). METHODS: The PGE2 concentration in supernatants of HMC was measured by radioimmunoassay. COX-2 mRNA and protein expression were determined by RT-PCR and Western blot. Electrophoretic mobility shift assay (EMSA) and Western blot were used to detect the activity of NF-κB and degradation of IκB. RESULTS: IL-1β significantly upregulated COX-2 expression and PGE2 production in HMC. Significant up-regulation of NF-κB activation, nuclear translocation of p65 subunit, and degradation of IκB α and IκB β were observed in IL-1β-induced HMC. CONCLUSION: Expression of COX-2 in IL-1β-induced HMC is mediated by NF-κB/IκB signal pathway. 相似文献
119.
AIM: To investigate the influence of Ginkgo biloba extract (EGb761) on c-jun expressions and motoneurons survival following root avulsion. METHODS: One hundred and eighty adult Sprague-Dawley female rats were randomly divided into control and EGb761 groups. Immediately after avulsion of C5-T1 nerve roots, the rats were injected ip with either 1 mL of EGb761 25 mg·kg-1·d-1 or the same volume of normal saline, and the treatment repeated everyday. At 4 h to 6 weeks following avulsion, the C7 spinal segments of all rats were collected and prepared for c-jun immunocytochemistry and neutral red stain. The numbers of c-jun positive and survival motoneurons were counted and compared between two groups at each time point. RESULTS: In control rats following avulsion, c-jun positive motoneurons appeared at 4 h, reached its maximum at 1 d and declined to 2 weeks. Avulsion-induced motoneurons death started at 2 weeks, climbed to its maximum at 4 weeks-6 weeks. In EGb761 treated rats, both numbers of c-jun positive and survival motoneurons were more than that in control group at each time point. CONCLUSION: EGb761 attenuates avulsion-induced motoneurons death, and this effect may be related to up-regulation of c-jun gene in avulsed motoneurons. 相似文献
120.
AIM: To explore the role of Akt signal pathway in apoptosis of neural cells in adult rats treated with Zuogui Pill, a Chinese medicine. METHODS: Flowcytometry and Western blotting methods were used to investigate the changes of cellular apoptosis rate and Akt signal pathway. RESULTS: Monosodium glutamate (MSG) could increase cellular apoptosis rate and significantly restrained the phosphorylation of Akt (Ser473) and Akt (Thr308), and markedly increased the levels of phospho-FKHR (ser256), GSK-3β (Ser9) and PTEN. Zuogui Pill partly inhibited the above effects of MSG. CONCLUSION: Zuogui pill effectively inhibits the neural apoptosis induced by MSG, and Akt pathway is involved in the neuronal protection of Zuogui pill. 相似文献