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131.
L.H. Wang X.H. Gu M.Y. Hua S.L. Mao Z.H. Zhang D.L. Peng X.F. Yun B.X. Zhang 《Scientia Horticulturae》2009
The root-knot nematodes (Meloidogyne spp.) are important nematode pests and cause serious diseases in pepper in the world. No molecular markers linked to the nematodes resistance N gene have been reported. In this paper, ‘Carolina Wonder’ (Capsicum annuum L.), a sweet pepper line resistant to root-knot nematode with N gene, ‘20080-5-29’ (C. annuum L.), an inbred line susceptible to root-knot nematode with good horticultural characteristics, and their F2 progeny with 320 individuals were used as materials. Evaluation of resistance and susceptibility of parental lines, F1 and F2 progeny inoculated with root-knot nematodes (Meloidogyne incognita) were carried out. ‘Bulked segregant analysis’ method was used to search for polymorphic markers from 512 pairs of AFLP primers. Based on the assessment of resistance and susceptibility and polymorphism of the AFLP marker in F2 population, the genetic linkage distance between the AFLP marker and the N gene was estimated. One AFLP marker E39/M41-339 was obtained and transferred to a SCAR marker amplifying a 315 bp DNA fragment linked to the N resistant allele and a 331 bp fragment linked to the N+ susceptible allele. The distance between the molecular marker and the nematodes resistance N gene is 6.3 cM. This research delivered a valuable tool for the marker assisted selection of nematodes resistance in pepper. 相似文献
132.
J.J. MA Z.R. XU Q.J. SHAO J.Z. XU SILAS S. O. HUNG W.L. HU L.Y. ZHUO 《Aquaculture Nutrition》2008,14(5):464-471
Effects of dietary l ‐carnitine were studied in juvenile black sea bream (Sparus macrocephalus). The semipurified basal diet [crude protein 450 g kg?1 dry matter (DM); crude lipid 126 g kg?1 DM] was formulated to choose white fishmeal as the protein source and fish oil plus corn oil (1 : 1) as the lipid source. Six diets (control + diets 1–5) containing 0.1, 0.12, 0.16, 0.24, 0.39 and 1.1 g of l ‐carnitine kg?1 diet were fed to triplicate groups of black sea bream (initial weight 13.10 ± 0.05 g) for 8 weeks. At the end of the feeding trial, growth performance, body composition and antioxidant status were determined. The results showed that relative growth rate (RGR) was significantly improved by the elevation of dietary l ‐carnitine level from 0.1 to 0.24 g kg?1, but decreased with further increment (P < 0.05). Lipid content decreased significantly (P < 0.05) in the dorsal muscle whereas increased (P < 0.05) in the liver with the addition of dietary l ‐carnitine. Dietary l ‐carnitine supplements elevated enzymatic antioxidants (superoxide dismutase, SOD; catalase, CAT; glutathione‐S‐transferase, GST) activities (P < 0.05) yet decreased the content of non‐enzymatic factor, total sulphydryl groups (TSH) (P < 0.05). In summary, the optimum dietary l ‐carnitine level was 0.284 g kg?1 diet by second‐polynomial regression analysis based on RGR (y = ?647.4x2 +367.97x + 234.55; R2 = 0.977, x = dietary l ‐carnitine levels, y = RGR), and dietary l ‐carnitine addition within the levels adopted in our study could depress lipid peroxidation in tissues of juvenile black sea bream. 相似文献
133.
Q.H. AI J.Z. ZHAO K.S. MAI W. XU B.P. TAN H.M. MA Z.G. LIUFU 《Aquaculture Nutrition》2008,14(6):515-522
A 30‐day feeding experiment was conducted in blue tanks (70 × 50 × 60 cm, water volume 180 L) to determine the effects of dietary lipid levels on the survival, growth and body composition of large yellow croaker (Pseudosciaena crocea) larvae (12 days after hatchery, with initial average weight 1.93 ± 0.11 mg). Five practical microdiets, containing 83 g kg?1 (Diet 1), 126 g kg?1 (Diet 2), 164 g kg?1 (Diet 3), 204 g kg?1 (Diet 4) and 248 g kg?1 lipid (Diet 5), were formulated. Live feeds (Artemia sinicia nauplii and live copepods) were used as the control diet (Diet 6). Each diet was randomly assigned to triplicate groups of tanks, and each tank was stocked with 3500 larvae. During the experiment, water temperature was maintained at 23(±1) °C, pH 8.0 (±0.2) and salinity 25 (±2) g L?1. The results showed that dietary lipid significantly influenced the survival and growth of large yellow croaker larvae. Survival increased with the increase of dietary lipid from 83 to 164 g kg?1, and then decreased. The survival of larvae fed the diet with 83 g kg?1 lipid (16.1%) was significantly lower than that of larvae fed other diets. However, the survival in larvae fed the diet with 16.4 g kg?1 lipid was the highest compared with other artificial microdiets. Specific growth rate (SGR) significantly increased with increasing dietary lipid level from 83 to 164 g kg?1 (P < 0.05), and then decreased. The SGR in larvae fed the diet with 164 g kg?1 lipid (10.0% per day) was comparable with 204 g kg?1 lipid (9.6% per day), but were significantly higher than other microdiets (P < 0.05). On the basis of survival and SGR, the optimum dietary lipid level was estimated to be 172 and 177 g kg?1 of diet using second‐order polynomial regression analysis respectively. 相似文献
134.
A.C.B. OLIVEIRA L.A. MARTINELLI M.Z. MOREIRA & J.E.P. CYRINO 《Aquaculture Nutrition》2008,14(1):10-13
Estimation of the apparent digestibility coefficient ADC(%) of C3 and C4 plants in experimental diets for fingerlings of tambaqui (Colossoma macropomum, Cuvier, 1818) was calculated by applying chromic oxide (Cr2O3) external marker methodology and by a proposed mathematical expression based on the isotopic composition (δ13C). A total of 240 tambaqui fingerlings each weighing ±48.2 g and measuring ±9.8 cm were maintained in eight 500‐L aquariums specially designed for faeces collection. The ADC(%) of the C3 and C4 diets did not differ significantly between the two methods, producing results of 75.6%; 76.2% and 74.4%; 72.8%, respectively. The ADC(%) results obtained by isotopic method presented less variation than by chromic oxide. The proposed mathematical expression for calculating the ADC(%) based on δ13C values offers an alternative methodology, which can reduce errors and diminish the effort required to collect biological material. However, it is important to note that this method is limited to analysis of diets or food items with distinct isotopic signals. 相似文献
135.
Clostridium butyrium CB2 isolated from chickens was tested as a potential fish probiotic in the Chinese drum, Miichthys miiuy . Fish were fed live (CB), dead CB2 (D-CB) cells (108 cells g−1 ) or spent culture supernatant (SCS), for 30 days and challenged with Vibrio anguillarum or Aeromonas hydrophila . Survival was higher in both the CB and the CB-D fed groups, but the SCS group was not significantly different from the control. After feeding live or dead CB2 cells, there was increase in phagocytic activity of the head kidney macrophages, the lysozyme activity of serum and gut mucosa and immunoglobulin (Ig) level. The SCS group showed no obvious change in immune parameters. The results suggest that live or dead Clostridium butyrium CB2 has an immunomodulatory effect on fish. 相似文献
136.
137.
水稻对稻瘟病抗性的分子生物学研究进展 总被引:17,自引:0,他引:17
介绍了水稻对稻瘟病抗性的分子生物学研究进展,主要包括:用于定位作图的分子标记的种类和特点、稻瘟病抗性基因的分子标记定位、稻瘟病抗性基因的等位性比较研究、稻瘟病菌生理小种无毒基因的克隆以及稻瘟病抗性基因克隆研究进展等。 相似文献
138.
Effects of rake angle of chisel plough on soil cutting factors and power requirements: A computer simulation 总被引:1,自引:0,他引:1
A computer simulation was conducted to predict the effects of rake angle of a chisel plough and soil bulk density on angle of soil failure plane, rupture distance, width of side crescent, frictional, overburden, cohesion and adhesion soil cutting factors, draft forces and drawbar power requirements. The experimental work was carried out in two locations. Soil of the first location was sandy clay with the soil bulk densities of 1.75 and 1.70 g/cm3 for firm and loose soil conditions, respectively, with an angle of internal friction of 30° and a surface friction angle of 20°, cohesion of 2.5 kN/m2 and adhesion of 1.2 kN/m2. Soil of the second location was clay loam with the soil bulk densities of 1.65 and 1.50 g/cm3 for firm and loose soil conditions, respectively, with an angle of internal friction of 34° and a surface friction angle of 23°, cohesion of 2.4 kN/m2 and adhesion of 1.14 kN/m2. The prediction showed that the angle of failure plane found to decrease with the rake angle. The rupture distance decreased with the rake angle from 15° to 55° and then increased as the rake angle increased over 55°. The width of the side crescent increased as the rake angle increased and the maximum value and the minimum value were recorded at 75° and at 15°. Values of frictional and overburden factors decreased as rake angle increased. The maximum and minimum values were recorded at 15° and 75°, respectively. The values of cohesion factor increased as rake angle increased. The maximum value was recorded at rake angle of 75° and the minimum value was recorded at rake angle of 15°. Adhesion factor was found to change inversely with the rake angle from 15° to 55° and then to change directly with the rake angle over 55°. The draft force decreased with the rake angle and reached its minimum value at 45° rake angle. Over 45°, the draft force increased and reached its maximum value at 75° rake angle. The draft increased with soil bulk density. The power required for moving the plough recorded the maximum value at rake angle of 15°, while the minimum value was recorded at 55° rake angle. The values of power increased with decrease of soil bulk density. The predicted values demonstrated some deviations from the experimental values of the draft force and the drawbar power. 相似文献
139.
Distribution of light and heavy fractions of soil organic carbon as related to land use and tillage practice 总被引:10,自引:0,他引:10
Mass distributions of different soil organic carbon (SOC) fractions are influenced by land use and management. Concentrations of C and N in light- and heavy fractions of bulk soils and aggregates in 0–20 cm were determined to evaluate the role of aggregation in SOC sequestration under conventional tillage (CT), no-till (NT), and forest treatments. Light- and heavy fractions of SOC were separated using 1.85 g mL−1 sodium polytungstate solution. Soils under forest and NT preserved, respectively, 167% and 94% more light fraction than those under CT. The mass of light fraction decreased with an increase in soil depth, but significantly increased with an increase in aggregate size. C concentrations of light fraction in all aggregate classes were significantly higher under NT and forest than under CT. C concentrations in heavy fraction averaged 20, 10, and 8 g kg−1 under forest, NT, and CT, respectively. Of the total SOC pool, heavy fraction C accounted for 76% in CT soils and 63% in forest and NT soils. These data suggest that there is a greater protection of SOC by aggregates in the light fraction of minimally disturbed soils than that of disturbed soil, and the SOC loss following conversion from forest to agriculture is attributed to reduction in C concentrations in both heavy and light fractions. In contrast, the SOC gain upon conversion from CT to NT is primarily attributed to an increase in C concentration in the light fraction. 相似文献
140.
Nitrogen Accumulation and Translocation for Winter Wheat under Different Irrigation Regimes 总被引:12,自引:0,他引:12
The translocation of pre‐anthesis nitrogen to the grain is an important source for winter wheat. The relation between the nitrogen translocation and irrigation regime was studied in the field under a rain‐proof trough shelter. Nitrogen (N) translocation amount, N translocation efficiency decreased with a decline in irrigation amount or by excessive irrigation. Compared with different organs, the leaf and stem had higher N translocation amounts, and contributions to grain for both cultivars – Jinan 17 and Lumai 21, indicating that stem also is a major N source for grain development. The contribution of pre‐anthesis total above ground N to grain N ranged from 57 to 76 %, indicating the importance of pre‐anthesis storage of N for achieving high grain N concentrations. Grain nitrogen and yield (kg ha?1) were positively and significantly correlated with the N translocation amounts and contributions, respectively, suggesting that the sink strength may be involved in the translocation of N from a vegetative organ to the grain. N harvest index (NHI) was significantly correlated with N translocation efficiency, suggesting that the latter is a prerequisite for increasing grain N and improving grain quality. The experiment showed that N translocation status is enhanced by better irrigation practices, but limited by severely deficient or excessive irrigation. 相似文献