利用16S rRNA全长序列鉴定植物乳杆菌

从标明含有嗜酸乳杆菌的活菌胶囊中分离到一株乳杆菌Lal菌株，利用16S rRNA全序列分析法和传统分类法对Lal菌株进行分类鉴定表明：Lal菌株属于植物乳杆菌种新株系，该菌株现在已经被中国科学院北京微生物所菌种保藏中心收录保存，编号为ASl．2986。     

An Expert System Approach to the Computer Aided Design of Intelligent Controllers

The idea for an expert system approach to the computer aided design of intelligent controllers (ESCAD1C) is discussed and the idea of two step modelling of an intelligent controller is presented on the basis of emulating human control behaviours to solve complex problems. The structure, functions and characteristics of ESCADTC system , and the design procedures of intelligent controllers are gived. The knowledge base and simulating functions for designing intelligent controllers are also described.     

A Study on Human Simulation Intelligent Control Algorithm to a Category of Objects

Based on the model of Human Simulation Intelligent Controller (HSIC) presented briefly, a detailed algorithm was described for the servo system control by using the method of HSIC. Simulation results obtained proves the good performance of the control algorithm.     

MACHINE PARAMETER IDENTIFICATION AND FAULT DETECTION FOR THE ALTERNATOR SYSTEM

In this paper, the machine( physical) parameters of the alternator system are on-line estimated on the basis of the derived system continuous-tims model, using Recursive Least-squares Estimation and Inverse Calculation Method proposed by Authors. Therefore, the estimated machine parameters can effectively track the changes of their true values and accomplish the fault detection for the alternator system. This paper gives a new way for machne parameter estimation and fault detection of the alternator systems.     

苹果水心病的光学无损检测

通过密度法和自行研制的水心病检测仪器对苹果样品进行检测 ,结果表明 :密度无法分离该样品水心病 ;而 1～ 4级水心病的透光强度之间存在显著差异 ,可以进行水心病判断。把近红外 810 nm的计算透光强度2 5 0 0 0作为界限值可以将好果 (1级 )与 3、4级果完全分离 ,2级果有 2 9%分离     

鄱阳湖区反嘴鹬种群数量动态与空间分布格局

2002—2015年,采用同步调查法对鄱阳湖区反嘴鹬(Recurvirostra avosetta)越冬种群数量动态与空间分布格局进行了监测。结果表明:鄱阳湖区分布有稳定的反嘴鹬越冬种群,年平均数量为(16684±14653)只,2004年、2005年、2013年种群数量均超过目前其全球数量的10%,2004年冬季鄱阳湖区越冬反嘴鹬数量达到最大值,为47520只。鄱阳湖区共有70个湖泊记录到反嘴鹬,其中反嘴鹬种群数量达到全球1%以上的湖泊有20个。每年冬季鄱阳湖区反嘴鹬集中分布在常湖、蚌湖、花庙湖和下坝湖,而反嘴鹬利用频次最高的是大湖池,每年反嘴鹬的越冬数量为(1819±1593)只。各湖泊中反嘴鹬的年平均数量与利用频次之间不存在显著相关性。自然保护区涵盖了反嘴鹬在鄱阳湖越冬的主要湖泊,分布在保护区内的反嘴鹬种群占鄱阳湖区越冬总数量的(75.3±26.0)%,但仍有很多重要的湖泊没有被纳入到保护区范围。反嘴鹬对保护区内湖泊的利用频次与保护区外湖泊的利用频次不存在显著性差异,保护区内反嘴鹬的种群数量显著大于保护区外。     

Saccharobisindole,Neoasterric Methyl Ester,and 7-Chloro-4(1H)-quinolone: Three New Compounds Isolated from the Marine Bacterium Saccharomonospora sp.

Analysis of the chemical components from the culture broth of the marine bacterium Saccharomonospora sp. CNQ-490 has yielded three novel compounds: saccharobisindole (1), neoasterric methyl ester (2), and 7-chloro-4(1H)-quinolone (3), in addition to acremonidine E (4), pinselin (5), penicitrinon A (6), and penicitrinon E (7). The chemical structures of the three novel compounds were elucidated by the interpretation of 1D, 2D nuclear magnetic resonance (NMR), and high-resolution mass spectrometry (HRMS) data. Compound 2 generated weak inhibition activity against Bacillus subtilis KCTC2441 and Staphylococcus aureus KCTC1927 at concentrations of 32 μg/mL and 64 μg/mL, respectively, whereas compounds 1 and 3 did not have any observable effects. In addition, compound 2 displayed weak anti-quorum sensing (QS) effects against S. aureus KCTC1927 and Micrococcus luteus SCO560.     

2BYS—6型水田中耕除草机设计与试验

简述了2BYS-6型水田中耕除草机的结构、工作原理及关键部件的设计,对该机旋转除草和摆动除草两种工作部件运动作了理论分析.田间试验结果表明:该机用于机插秧水稻田除草行间杂草平均除净率(相对除净率)78.1%,中耕深度4.39 cm,作物损伤率不大于6.89%.     

超临界脱除茶叶咖啡因的优化工艺

以完整茶叶为研究对象,通过单因素实验和正交实验研究萃取压力、萃取温度、夹带剂浓度、夹带剂用量4因素对咖啡因脱除率的影响,优化了超临界脱除茶叶咖啡因的工艺条件,即萃取压力35MPa、萃取温度80℃、浓度70%的乙醇作为夹带剂,其用量与茶叶的质量比为1.2∶1时脱除率最高,达到48.96,以此达到提高茶叶品质和价值的目的.     

Comparison of the chemical constituents of aged pu-erh tea, ripened pu-erh tea, and other teas using HPLC-DAD-ESI-MSn

Pu-erh tea is a popular beverage in southwestern China and South Asian countries. To explain the differences of aged pu-erh tea and ripened pu-erh tea, the chemical constituents of these teas were identified by HPLC-DAD-ESI-MS(n). In addition, HPLC was used to determine the contents of the major polyphenols, gallic acid, caffeine, and theobromine, in various types of teas. These results showed that the majority of chemical constituents in ripened pu-erh tea and aged pu-erh tea were similar, but the contents of catechins and gallic acid presented significant differences between these two teas. After fermentation by microorganism, the levels of catechins in ripened pu-erh tea were decreased, but the contents of gallic acid and caffeine were conversely elevated compared with aged pu-erh tea.