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41.
贵阳市乌当区某个体养鸡场鸡群发病,诊断为鸡伤寒沙门氏菌不同血清型的菌株混合感染,药敏试验表明分离菌株对部分β-内酰胺类抗生素、四环素、氟喹诺酮类、红霉素、磺胺类具有不同程度的耐药性.笔者用中药黄芩、土大黄结合阿莫西林进行治疗,取得较好效果,现报道如下. 相似文献
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选用6个羟基红花黄色素A(HSYA)含量差异较大的亲本,采用双列杂交方法配制杂交组合,测定2015年和2016年2年亲本及其后代F1和F2红花中的HSYA含量。运用双子叶植物种子数量性状遗传模型和统计分析方法,分析胚、细胞质和母体植株3套遗传体系的基因效应和环境互作效应。结果发现:在HSYA含量的遗传体系中,母体遗传效应影响最大,胚效应次之,细胞质效应影响最小。3套遗传体系均表现出基因主效应大于环境互作效应。机误方差较大,说明HSYA含量还受环境机误或抽样误差的影响。亲本遗传效应分析表明,豫红花1号(P1)做亲本表现稳定,有利于增加杂交后代HSYA含量,达到提高品质、改良品种的效果。胚显性方差和母体显性方差均达到极显著水平,表明同时存在种子杂种优势和母体杂种优势,而且其主效应基因不受环境影响。综合考虑遗传主效应、胚显性效应和母体显性效应,亲本组合(P1×P5)有利于提高后代杂交品种的HSYA含量。该研究结果可为后代材料在杂种优势利用中的亲本选择提供理论支持。 相似文献
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为明确小热激蛋白在沙葱萤叶甲Galeruca daurica应对温度胁迫中的作用,采用PCR方法克隆沙葱萤叶甲小热激蛋白基因Hsp20(GdHsp20.6)完整的开放阅读框(open reading frame,ORF)序列,应用在线软件对GdHsp20.6基因进行生物信息学分析,通过原核表达技术诱导表达及纯化其编码蛋白,并通过实时荧光定量PCR(real-time quantitative PCR,RT-qPCR)技术分析不同温度胁迫下GdHsp20.6基因的表达量。结果显示,GdHsp20.6基因ORF序列长度为543 bp,编码180个氨基酸,预测分子量为20.6 kD,无跨膜区和信号肽。GdHsp20.6氨基酸序列有高度保守的α-结构域。GdHsp20.6氨基酸序列与其它鞘翅目昆虫的Hsp20氨基酸序列有较高的一致性,其中与花绒寄甲Dastarcus helophoroides的Hsp20.99氨基酸序列的一致性最高,为63%。GdHsp20.6基因在大肠杆菌Escherichia coli BL21(DE3)细胞系中成功表达,经异丙基-β-D-硫代吡喃半乳糖苷(isopropyl-β-d-thiogalactoside,IPTG)诱导后GdHsp20.6蛋白成功表达,十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(sodium dodecyl sulfate-polyacrylamide gel electrophoresis,SDS-PAGE)和Western-blot分析表明融合蛋白大小与预测大小一致,并纯化获得了纯度较高的目的蛋白GdHsp20.6。低温(-10~5℃)和高温(35~40℃)处理1 h以及处理后25℃恢复30 min均能诱导GdHsp20.6基因表达上调,并且0℃处理30~120 min也能诱导GdHsp20.6基因表达上调。表明GdHsp20.6基因在沙葱萤叶甲应对低温和高温胁迫中可能起着重要作用。 相似文献
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Qinghui Ai Kangsen Mai Beiping Tan Wei Xu Qingyuan Duan Hongming Ma Lu Zhang 《Aquaculture (Amsterdam, Netherlands)》2006,260(1-4):255-263
A growth experiment was conducted to investigate the effects of replacement of fish meal (FM) by meat and bone meal (MBM) in diets on the growth and body composition of large yellow croaker (Pseudosciaena crocea). Six isonitrogenous (43% crude protein) and isoenergetic (20 kJ g− 1) diets replacing 0, 15, 30, 45, 60 and 75% FM protein by MBM protein were formulated. Each diet was randomly allocated to triplicate groups of fish in sea floating cages (1.0 × 1.0 × 1.5 m), and each cage was stocked with 180 fish (initial average weight of 1.88 ± 0.02 g). Fish were fed twice daily (05:00 and 17:30) to apparent satiation for 8 weeks. The water temperature ranged from 26.5 to 32.5 °C, salinity from 32 to 36‰, and dissolved oxygen content was approximately 7 mg l− 1 during the experimental period. Survival decreased with increasing dietary MBM and the survival in the fish fed the diet with 75% protein from MBM was significantly lower than other groups (P < 0.05). There were no significant differences in specific growth rate (SGR) among the fish fed the diets with 0 (the control group), 15, 30 and 45% protein from MBM. However, SGR in the fish fed the diets with 60 and 75% protein from MBM were significantly lower than other groups (P < 0.05). No significant differences in feeding rate were observed among dietary treatments. The digestibility experiment showed that the apparent digestibility coefficients (ADC) of dry matter, protein, lipid and energy of MBM were significantly lower compared with those of FM (P < 0.05). Essential amino acid index was found to be correlated positively with SGR in the present study, suggesting that essential amino acid balance was important. Body composition analysis showed that the carcass protein and essential amino acids were not significantly affected by dietary MBM. The lipid and n-3 highly unsaturated fatty acid (n-3 HUFA) in fish muscle, however, significantly decreased with increasing dietary MBM. These results showed that 45% of FM protein could be replaced by MBM protein in diets of large yellow croaker without significantly reducing growth. It was suggested that the reduced growth with higher MBM was due to lower digestibility and imbalance of essential amino acids. 相似文献
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Qianqian Huang Heizhao Lin Ruixuan Wang Zhong Huang Chuanpeng Zhou Wei Yu Pengwei Xun Lianjie Tan Yun Wang Jun Wang 《Aquaculture Research》2019,50(9):2359-2370
In order to investigate the dietary vitamin B6 (VB6) requirement for juvenile golden pompano, an experiment of six different diets with six dietary VB6 levels (0, 3.75, 7.47, 10.6, 13.7 and 18.5 mg/kg) was conducted. The results indicated that the content of dietary VB6 significantly increased weight gain rate (WGR), specific growth rate (SGR) and feed conversion ratio (FCR; p < 0.05). Golden pompano fed diet containing 7.47 mg/kg VB6 achieved the maximum ALT, AST, GPx, hepatic VB6 concentrations as well as the minimum MDA, however, the ALT, AST, GPx and hepatic VB6 concentrations decreased while MDA increased with a further increase in dieatry VB6. The highest values of GR and POD occurred at the 10.6 and 13.7 mg/kg dietary VB6 (p < 0.05) respectively. A diet supplemented with 7.47 mg/kg VB6 increased intestinal Na+, K+‐ATPase, Chymotrypsin, γ‐glutamyl transpeptidase, creatine kinase and amylase activities (p < 0.05). The relative abundance of Tenericutes and Bacteroidetes decreased while Proteobacteria and Firmicutes improved with an increase in dietary VB6 levels up to 7.47 mg/kg. Quadratic regression analysis on WGR, hepatic VB6 concentrations, AST and ALT indicated that the optimum dietary VB6 levels for juvenile golden pompano were 8.84–9.28 mg/kg. 相似文献
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