实验性急性猪丹毒抗原定位和病理学研究

用猪丹毒强毒株人工接种试验猪,采取其心、肺、肾、肝等器官制成样品,光镜和电镜观察结果,靶器官为心、肾、脑、脾、淋巴结,猪丹毒杆菌定位在器官的微血管。     

蚕沙中黄酮类化合物的提取工艺

采用L9(3^4)正交试验设计对浙江海宁蚕沙中黄酮类化合物的热回流提取工艺进行了优化，所得出最佳工艺条件为；乙醇浓度为30％，料液比1：30，提取时间3h。     

Effect of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponense

This study examined the effects of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponese . The response of the cellular defense was determined by monitoring changes in reactive oxygen intermediates in hemocytes, superoxide dismutase activity, catalase activity, glutathione peroxidase activity, glutathione reductase and glutathione transferase activity in muscle. The results showed that prawns fed the basal diet had a significant increase in reactive oxygen intermediates in response to exposure to ammonia-N. When dietary vitamin C was added to the feed, there was a significant decrease in reactive oxygen intermediates in response to exposure to ammonia-N. Total superoxide dismutase and glutathione peroxidase activity of prawns fed the basal diet decreased as ammonia-N increased. This decrease in activity was significantly less pronounced in prawns that were fed the dietary vitamin C supplement. In prawns that were fed the dietary vitamin C supplement there was no significant variation in catalase reductase or gluxathione reductase activity in response to variations in ammonia-N concentration. The glutathione transference activity increased at a low concentration of ammonia-N in prawns that were fed the dietary vitamin C supplement, but there was no significant variation in the prawns fed the basal diet. Overall the results indicate that dietary vitamin C supplements enhance the capacity of M. nipponese to respond to elevations in ammonia-N. This response involved several biochemical reactions known to be involved in cellular defense.     

压力实时监测法在管道泄漏检测中的应用

检测输油管道漏油的方法很多，介绍了通过压力实时监测确定输油管道漏油点方法，以及实际应用情况。实践证明，通过压力实时监视确定输油管道漏油点的方法在实际输油运行中是切实可行的。     

表面技术在内燃机气缸套中的新进展

论述了气缸套的失效机理，针对这一缺点，较全面地阐述了当前表面技术在气缸套上的应用现状，并提出了未来气缸套的发展方向。为同行提供了参考和启迪，也为拓宽热喷涂技术的应用指明了一个方向。     

PRRSV弱毒细胞疫苗与灭活疫苗生殖道黏膜免疫效果观察

应用组织学方法比较观察了猪繁殖与呼吸综合征病毒(PRRSV)弱毒细胞疫苗与灭活疫苗生殖道黏膜免疫的效果。试验选用5月龄长白×大约克夏二元杂交母猪18头。PRRSV弱毒细胞疫苗和灭活疫苗通过生殖道黏膜免疫接种。结果表明PRRSV弱毒细胞疫苗生殖道黏膜接种组母猪的子宫黏膜上皮内淋巴细胞数较灭活疫苗生殖道黏膜免疫接种组和对照组母猪显著减少(P<0.01);而且子宫黏膜上皮变矮变薄,变得有些不规则。灭活疫苗生殖道黏膜接种组母猪子宫黏膜上皮内淋巴细胞数与对照组母猪间差异不显著(P>0.05)。本试验结果显示PRRSV弱毒细胞疫苗经生殖道黏膜免疫接种则对生殖道黏膜上皮内淋巴细胞产生抑制作用。说明PRRSV弱毒细胞疫苗和灭活疫苗经生殖道黏膜免疫效果不理想     

熵产生原理和耗散结构

本文讨论了熵产生原理和耗散结构理论的基本概念和有关问题,以及上述理论在生物学中的应用。     

从空育131的试种成功看寒地粳稻种质资源的创新

由于空育131熟期适宜、稳产、丰产、优质、耐寒、抗倒等特点,近几年在黑龙江省种植面积处于领先地位。但随着种植年限的增加,稻瘟病有加重的趋势,应对其正确评价。针对寒地水稻育种缓慢的主要原因———种质资源的贫乏落后,提出了资源创新两种主要途径———自已创新及外引优异种质资源。     

如何加强市场经济条件下工程造价管理

本文根据目前工程造价管理的实践，指出了存在的问题，并提出了如何加强市场经济条件下建设工程造价管理的四点具体意见，很有现实意义和指导意义。     

一种快速分析香蕉果实采后mRNA水平变化的新方法

采用SMARTPCRcDNA合成与计算机软件相结合的方法,快速分析香蕉果实采后mRNA水平的变化。在28℃条件下分别将处于同株同穗同梳相邻位置的香蕉果实放置24、48、72h。应用这种方法对其总mRNA水平进行了分析,结果表明,香蕉采后48h时,与采后0h相比,mRNA水平最高。该方法由于使用了计算机软件TheScionImage,可对cDNA电泳图进行数字化分析,具有用材少,敏感性高,快捷方便等特点,特别适用于对较多样品进行分析。