Zinc Ameliorates Fruit Yield and Quality of Mangoes Cultivated in Calcareous Soils

Zinc (Zn) deficiency is frequently observed in mangoes grown in calcareous soils. Inherent inability of mango to extract sufficient Zn from the soil exacerbates this situation. Therefore, mitigating effects of Zn application was investigated on flowering, fruit setting, yield and quality of mango cv. Samar Bahisht Chaunsa growing in calcareous soils. The experiment consisted of five treatments: control—no Zn, soil application of 50 and 100 g ZnSO₄ per tree and foliar application of 0.5? and 1.0?% ZnSO₄ solution. Zn was applied twice in the 2^nd week of November, 2011 and 3^rd week of March, 2012, respectively. The experiment was repeated on the same dates during the next growing season. Zn application significantly increased number of flower panicle, fruits matured panicle, leaf Zn concentrations, fruit size, peel thickness, fruit weights at harvest and after ripening and fruit yield tree, whereas it reduced malformed panicles and early fruit drop. However, flower sex ratio and fruit set remained statistically unaffected. Similarly, fruit quality in terms of total soluble solids, flesh color, aroma, flavor and overall acceptability significantly improved as compared to control. Foliar application mitigated Zn deficiency more effectively than the soil application in calcareous soils.     

Demography of the threatened endemic shrub, <Emphasis Type="Italic">Arbutus pavarii,</Emphasis> in the Al-Akhdar mountainous landscape of Libya

We sampled twenty populations of the vulnerable endemic shrub or tree, Arbutus pavarii Pampan., at different elevations and aspects within the Al-Akhdar mountainous region of Libya. Our sampling sites were at elevations ranging from 285 to 738 m above sea level, and several different habitats: vallies (locally known as wadis), north- and south-facing slopes, and mountaintops. All individuals within each quadrat were studied. Population size and structure, and plant functional traits were assessed. None of the populations had a stable distribution of size classes. Some consisted mostly of small plants, with little or no fruit production; others consisted only of mid-sized and large plants, with high fruit production, but no juvenile recruitment. There was a significant increase in percent cover with increasing elevation; reproductive output (the number of fruits per branch and total number of fruits per individual) also generally increased with elevation. In some of these populations the lack of recruitment, and in others the failure to produce fruit, together constituted serious demographic threats. In light of these results, recommendations are made for conservation of this vulnerable endemic species.     

The Impact of Development Plans on Hydrological Changes in the Shazand Watershed,Iran

Development plans are mainly responsible for population changes and the conversion of forest and rangelands into agricultural lands and human settlements. Qualitative and quantitative analysis of population and land use changes are necessary to assess the impacts of change on hydrological processes. However, such important issues have been less considered worldwide particularly in developing countries. Therefore, we selected the Shazand Watershed (1740 km²) because of rapid industrialization to track the effects of land use and population changes on streamflow and sediment yield. The data were collected from statistical yearbooks and satellite imageries from 1973 to 2008. All available measurements on discharge and suspended sediment concentration at the Pole doab hydrological station were also collected. The study was conducted for the whole period, as well as the pre‐1991 and post‐1991 as a basis for the economic development growth in the region. We found that the land use and population changes have occurred in the Shazand Watershed, especially in the vicinity of industrial zones. The results showed that the cities, industrial zones, roads, and bare lands quickly increased from 58 · 8 to 134 · 3 km² during post‐1991. The flow durations, sediment rating curves and trend analyses indicated distinct variations in the relationship between streamflow and sediment and also caused changes within different periods. Based on the results, the mean annual flow and sediment yield in post industrialization (1991–2008) were respectively 0 · 84 and 1 · 19 times of those for pre‐industrialization period and the annual sediment yield increased from 25,000 to 29,850 Mg. Copyright © 2016 John Wiley & Sons, Ltd.     

Cloning and expression of coxsakievirus B3 viral protein-1 in E. coli

BACKGROUND: Viral protein-1 (VP1) is a major capsid protein of Coxsakievirus B3 (CVB3) that plays an important role in directing viruses towards permissive cells and acts as a main antigenic site of the virus in eliciting of host immune response, hence it seems VP1 can be considered as a vaccine candidate against CVB3 infection. In this study, cDNA of VP1 was prepared, cloned into pET expression vector and the recombinant protein (VP1) was over expressed in E. coli. METHODS: The viruses were grown in suspension cultures of Vero cells with an input virus multiplicity of 10-50 plaque-forming units/cell. After observing complete cytopathic effect, the total RNA (cells and virus) was prepared for RT-PCR and by using specific primers, VP1 cDNA was amplified and ligated into pET vectors (32 a and 28 a). The recombinant vector was transferred into competent E. coli (BL-21) and after selection of proper colony, which carried correct cDNA within the vector; cells were cultured and induced with isopropyl B-D-thiogalactopyranoside, in order to express protein (VP1). The cultures were tested for presence of VP1 by SDS-PAGE and Western-Blotting analysis. RESULTS: Molecular techniques such as PCR which showed exact defined size of the VP1 (819 bp), restriction digestion and finally immunoblot analysis of over expressed protein; all confirmed the correct cloning and expression of VP1 in this research. CONCLUSION: In this research, full length of VP1 as major capsid protein of CVB3 was over expressed in E. coli which, can be used for further studies, including neutralizing antibody production against CVB3.     

Acylated flavonol glycoside from Platanus orientalis

The ethylacetate and n-butanol fractions of ethanolic extract of Platanus orientalis leaves led to the isolation of new acylated flavonol glycoside as 3',5,7-trihydroxy-4'-methoxyflavonol 3-[O-2-O-(2,4-Dihydroxy)-E-cinnamoyl-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranosyl (1→2)]-β-D-glucopyranoside, along with seven known compounds. All the compounds were characterized by NMR including 2D NMR techniques. The isolates were evaluated for NF-κB, nitric oxide (NO), aromatase and QR2 chemoprevention activities and some of them appeared to be modestly active.     

Prevalence and molecular characterization of bovine Cryptosporidium in Qazvin province, Iran

The aim of this study was to determine the prevalence, variability with host age, and the genotypes of species of Cryptosporidium in cattle from 15 dairy farms in Qazvin province, Iran. Fecal samples, collected from 272 cattle during May 2006 to December 2007, were characterized microscopically. Oocysts from 51 positive samples were analyzed using PCR assay of 18S SSU rRNA, restriction fragment length polymorphism (RFLP) and sequencing. We identified 72.6% of the positive samples as Cryptosporidium parvum, 17.7% as Cryptosporidium andersoni, 7.8% as Cryptosporidium bovis and 1.9% as a novel genotype of C. parvum possessing a single mutation on MboII restriction. An infection rate of 19.5% of C. parvum among 174 pre-weaned calves was significantly higher than the 3.1% among 98 post-weaned calves (P < 0.0006). This is the first report of C. bovis and the new subgenotype of C. parvum in Iranian cattle.     

Effects of plant residue management on soil properties,surface runoff,and soil loss under rainfall simulation in a semi-arid region in Iran

A 5-year field study was conducted on the effect of plant residue management on soil erosion, runoff, bulk density, penetration resistance, and organic carbon. There were three treatments: burning residues on field (BR), returning residues unto the soil surface after harvesting (TR), and removing residues from soil surface (RR) (control treatment). At the end of the 5-year treatment, a rainfall simulation at 90?mm?h^?1 was applied to each plot. After rainfall simulation started, the runoff volume and soil loss amount in samples were collected at four sampling times. The results showed that the runoff volume for BR (from 145.5?±?12.2 to 190.0?±?11.8?mL) differed significantly (p?≤?0.01) from that of RR (from 32.3?±?5.5 to 67.5?±?11.1?mL) and TR (from 10.0?±?0.7 to 16.7?±?3.3?mL). A significant difference (p?≤?0.01) was also observed between RR and TR regarding runoff volume and soil loss amount in different sampling times, except for the first sampling time (2–4?min). The runoff volume in BR and TR was 215% higher and 294% lower than that of the control (RR), respectively. As compared to the control (RR), soil loss decreased by 96.5% in TR but increased by 192% in BR. The BR increased soil bulk density and penetration resistance by 4.9% and 12.4%, whereas TR reduced them by 2.1% and 15.8%, respectively, as compared to the control (RR). The results indicated that the highest (0.35) and lowest (0.03) runoff coefficients were obtained for BR and TR treatments, respectively. It is concluded that returning plant residues to soil is the best residue management practice in decreasing soil runoff volume and controlling soil erosion in semi-arid regions.     

Comparison of three models describing bromide transport affected by different soil structure types

This study was conducted to investigate the effects of soil structure on bromide (Br) transport through three soils with granular, prismatic, and single-grain structures. The breakthrough curve (BTC) of the single-grain structure was sigmoidal, symmetrical and similar to a piston flow, showing the dominance of mass flow. In contrast, the BTCs of the granular and prismatic structures were initially steep, becoming more gradual at high pore volumes (PVs). The stable structure and preferential pathways caused the early breakthrough of Br in the leachate of these columns. The convection–dispersion equation (CDE), mobile–immobile water (MIM), and dual-permeability (DP) models were fitted to observed data using the program HYDRUS-1D. The equilibrium transport model (CDE) was not as successful as non-equilibrium (MIM and DP) models in describing the Br transport in prismatic and granular soil columns, although it was able to describe the Br transport in single-grain column well. Overall, the results demonstrated the importance of soil structure in pollutant transport through soils.     

利用水稻功能基因SSR标记鉴定水稻种质资源

 利用 16对水稻功能基因的SSR引物研究了 2 3份世界 5个国家不同来源的水稻种质资源的遗传多样性 ,共检测出 78个等位基因变异 ,每对引物可检测 2～ 10个等位基因变异 ,平均为 5 .2个 ,品种间遗传相似系数在0 .13～ 0 .6 4之间。UPGMA聚类分析表明 ,在相似系数 0 .13处可以将材料分为 2大类 ,来自巴西、日本和中国的粳稻全部聚为第Ⅰ类 ,巴西陆稻和原产科特迪瓦的陆稻聚在第Ⅰ类的第三小群 ;第Ⅱ类全为籼稻 ,来源于巴基斯坦的籼稻和 1个来源于韩国的籼稻分布在第Ⅱ类 ,说明水稻功能基因在不同亚种、不同产地来源和不同生态类型的水稻之间存在差异 ,也进一步证实水稻功能基因的SSR标记是研究水稻种质资源分类、地理分布、生态类型和系谱分析的有效工具。