Impaired respiratory and body temperature control upon acute serotonergic neuron inhibition

Physiological homeostasis is essential for organism survival. Highly responsive neuronal networks are involved, but their constituent neurons are just beginning to be resolved. To query brain serotonergic neurons in homeostasis, we used a neuronal silencing tool, mouse RC::FPDi (based on the synthetic G protein-coupled receptor Di), designed for cell type-specific, ligand-inducible, and reversible suppression of action potential firing. In mice harboring Di-expressing serotonergic neurons, administration of the ligand clozapine-N-oxide (CNO) by systemic injection attenuated the chemoreflex that normally increases respiration in response to tissue carbon dioxide (CO(2)) elevation and acidosis. At the cellular level, CNO suppressed firing rate increases evoked by CO(2) acidosis. Body thermoregulation at room temperature was also disrupted after CNO triggering of Di; core temperatures plummeted, then recovered. This work establishes that serotonergic neurons regulate life-sustaining respiratory and thermoregulatory networks, and demonstrates a noninvasive tool for mapping neuron function.     

Amplification and molecular cloning of HTLV-I sequences from DNA of multiple sclerosis patients

Techniques of gene amplification, molecular cloning, and sequence analysis were used to test for the presence of sequences related to human T-lymphotropic virus type I (HTLV-I) in peripheral blood mononuclear cells of six patients with multiple sclerosis (MS) and 20 normal individuals. HTLV-I sequences were detected in all six MS patients and in one individual from the control group by DNA blot analysis and molecular cloning of amplified DNAs. The viral sequence in MS patients were associated with adherent cell populations consisting predominantly of monocytes and macrophages. Molecular cloning and nucleotide sequence analysis indicated that these amplified viral sequences were related to the HTLV-I proviral genome.     

Antigen-specific proliferation and activation of peripheral blood mononuclear cells from Mycobacterium bovis-infected reindeer

To evaluate antigen-specific proliferative and activation-associated responses from Mycobacterium bovis-infected reindeer, blood mononuclear cells from M. bovis- (n = 10) and non-infected reindeer (n = 4) were stimulated with a recombinant early secretory antigenic target-6 and culture filtrate protein-10 fusion protein (rESAT6:CFP10), M. bovis purified protein derivative, pokeweed mitogen, or medium alone and evaluated by flow cytometry using dye tracker analysis and cell surface marker staining. gammadelta TCR+ and CD8+ cells, but not CD4+ cells, from M. bovis-infected reindeer proliferated in response to specific antigen stimulation. Expression (i.e., mean fluorescence intensity) of CD44 was increased and CD62L decreased on proliferative as compared to non-proliferative fractions in antigen- and mitogen-stimulated cultures. In response rESAT6:CFP10 stimulation, MHC II fluorescence intensity was increased on CD4+, gammadelta TCR+, CD172a+, and IgM+ cells from infected reindeer as compared to that of non-stimulated cells from the same reindeer. Recombinant ESAT6:CFP10 stimulation also induced expansion of a CD172a+, MHC II+ population within mononuclear cell cultures from M. bovis-infected reindeer. Despite a moderate challenge dose and extended duration of incubation, experimental infection of reindeer was generally limited to lymph nodes draining the inoculation site, suggestive of host resistance to progressive disease. Present in vitro findings, therefore, may be predictive of host responses by reindeer that limit progression to disseminated disease.     

Identification of gut‐associated amylase,cellulase and protease‐producing bacteria in three species of Indian major carps

Isolation and enumeration of amylase, cellulase and protease‐producing autochthonous bacteria in the proximal intestine (PI) and distal intestine (DI) of three species of Indian major carps, catla (Catla catla), mrigal (Cirrhinus mrigala) and rohu (Labeo rohita), were investigated using the conventional culture‐based technique. Population levels of amylolytic strains were the highest in the PI of catla and the lowest in the DI of rohu. The highest viable count of cellulase and protease‐producing bacteria was recorded in the DI and PI of mrigal respectively. Among the bacteria isolated, 10 strains (five from PI and five from DI) were selected as potent enzyme producers according to a quantitative enzyme assay. The chosen strains were further identified by 16S rRNA gene sequence analysis. The five strains isolated from catla showed high similarity to Citrobacter sp. clone W2, Enterobacter sp. JA24, Bacillus coagulans strain TR, uncultured bacterial clone Hel3bc04 and Bacillus cereus strain UST2006‐BC004. The four strains isolated from mrigal were most closely related to Bacillus sp. KCd2, uncultured bacterial clone Hel3bd09, B. cereus strain BU040901‐020 and Citrobacter freundii strain YRL11, while the strain isolated from rohu probably belonged to Bacillus sp. GV.     

Shrimp (Litopenaeus vannamei) production and stable isotope dynamics in clear‐water recirculating aquaculture systems versus biofloc systems

Closed recirculating aquaculture systems (RAS) offer advantages over traditional culture methods including enhanced biosecurity, the possibility of indoor, inland culture of marine species year‐round and potential marketing opportunities for fresh, never‐frozen seafood. Questions still remain regarding what type of aquaculture system may be best suited for the closed‐system culture of marine shrimp. In this study, shrimp (Litopenaeus vannamei) were grown in clear‐water RAS and in biofloc‐based systems. Comparisons were made between the system types with respect to water quality, shrimp production and stable isotope dynamics used to determine the biofloc contribution to shrimp nutrition. Ammonia and nitrite concentrations were higher, and shrimp survival was lower in the biofloc systems. Although stable isotope levels indicated that biofloc material may have contributed 28% of the carbon and 59% of the nitrogen in shrimp tissues, this did not correspond with improved shrimp production. Overall, the water column microbial communities in biofloc systems may be more difficult to manage than clear‐water RAS which have external filters to control water quality. Biofloc does seem to offer some nutritional contributions, but exactly how to take advantage of that and ensure improved production remains unclear.     

Air Pollution and its Impact on Lung Function of Children in Delhi, the Capital City of India

Air pollution is a major contributor to several respiratory problems, it affects the whole population in general but children are more susceptible. Exposure to automobile exhaust is associated with increased respiratory symptoms and may impair lung function in children. In view of this, the study was conducted among the children of Delhi, the capital city of India, where ambient air quality was much above the National Ambient Air Quality Standards. The study was conducted in children aged 9–17 years. Pulmonary function test was carried out following the guideline of American Thoracic Society using a portable, electronic spirometer. Air quality data was collected from Central and State Pollution Control Boards. In addition, the level of particulate matter in indoor air was measured by portable laser photometer. Lung function was reduced in 43.5% schoolchildren of the urban area compared with 25.7% of control group. The urban children had increased prevalence of restrictive, obstructive, as well as combined type of lung functions deficits. Besides higher prevalence, the magnitude of lung function deficits was also much more in them. After controlling potential confounders like season, socioeconomic conditions and ETS, PM10 level in ambient air was found to be associated with restrictive (OR?=?1.35, 95% CI 1.07–1.58), obstructive (OR?=?1.45, 95% CI 1.16–1.82), and combined type of lung function deficits (OR?=?1.74, 95% CI 1.37–2.71) in children. Spearman's rank correlation test reaffirmed the association. The study confirms that the level of air pollution is affecting the children.     

Effects of Population Density and Feeding Rate on Growth and Feed Consumption of Red Swamp Crawfish Procambarns clarkii

Wire-mesh enclosures were used in production ponds to conduct growth trials in which population density and feeding rate were evaluated in a 2 × 2 factorial arrangement for their effects on crawfish growth. Juvenile Procambarus clarkii of uniform size (0.5 g) were stocked at 2 or 20 animals/m² in experiment 1 and weighed biweekly for 12 wk. Rice forage served as the detrital base, and supplemental feed (25% crude protein) was provided at either a low (26–52 kg/ha per week) or high (104–418 kg/ha per week) rate. In experiment 2, mixed sizes of crawfish were stocked at 10/m² or 20/m² and fed the formulated feed at either 52 or 312 kg/ha per week. Feed consumption was estimated for each treatment combination. Only population density significantly affected crawfish growth. Mean weight gain and final weights were inversely proportional to density but only when total crawfish biomass exceeded 1,235 kg/ha. Growth was not significantly influenced by feeding rate, but the high feeding rate resulted in a significantly greater ratio of hepatopancreas weight to body weight and tended to decrease hepatopancreas moisture levels, indicating improved condition. Population density, feeding rate, and their interaction had significant effects on estimated feed consumption. From the simple linear regression of mean feed consumption on crawfish standing crop, it was estimated that crawfish consume about 0.1 kg of dry feed per ha per week per kg of crawfish biomass on a 5 d per week feeding schedule at mean water temperatures, ranging from 14 to 30 C.     

Remote sensing for agricultural water management and crop yield prediction

Research we have conducted over the past several years relative to agricultural application of remote sensing is reviewed. In addition, new data are presented from recent experiments reported here for the first time.The subjects treated are soil moisture, evaporation, irrigation scheduling, and crop yield estimation. The analyses indicate that we have the technology at hand to successfully integrate remote sensing techniques into agricultural operations designed to enhance production via intelligent water management.Avenues for additional fruitful research are indicated.