毛乌素沙地草地畜牧业经营模式的开发与示范

根据盐池县毛乌素沙地自然特点,坚持以发展种植业、畜牧业为主线,以科技创新为动力,开发出5种毛乌素沙地草地畜牧业经营模式并进行示范,以扩宽增加农民经济收入渠道,促进区域经济的发展,实现生态环境、经济、社会的协调发展。     

不同海拔芭蕉根药材UPLC-ELSD的指纹图谱分析

为更好地开发、利用芭蕉根药材,对不同海拔芭蕉根药材的UPLC-ELSD指纹图谱进行分析,采用Agilent ZORBAX RRHD Eclipse Plus C18(2.1 mm×100mm,1.8μm)色谱柱,0.1％乙酸水-乙腈洗脱系统,梯度洗脱,流速0.2 mL/min,蒸发光散射检测器(ELSD)检测,雾化温度为45℃,载气氮气流速1.5 L/min测定15批不同海拔的芭蕉根药材,对其进行聚类分析和相似度评价.结果表明:在不同海拔(298.52～1 357.82m)采集的芭蕉根药材的UPLC-ELSD指纹图谱有较好的相似度,芭蕉根中弱紫外吸收和缺少发色基团的化学成分的种类较统一；系统聚类分析可将芭蕉根药材归为4类,提示不同海拔的芭蕉根药材的质量存在较大差异.结论:建立的指纹图谱测定方法稳定、重复性好,可为芭蕉根药材的开发、利用及质量控制提供参考.     

Effect of Dietary Supplementation with Hydrolyzed Wheat Gluten on Growth Performance,Cell Immunity and Serum Biochemical Indices of Weaned Piglets （Sus scrofa）

To investigate the effects of dietary supplementation with hydrolyzed wheat gluten (HWG) on growth performance, cell immunity and serum biochemical indices of weaned piglets, 160 crossed (Large White×Landrace) and weaned piglets were randomly divided into 4 treatments with 4 replicates of 10 piglets each. The piglets in each treatment were fed an experimental diet containing either 0 g kg-1 HWG (control group), 30 g kg-1 HWG (3% HWG group), 50 g kg-1 HWG (5% HWG group), or 2.5 g kg-1 glycyl-L-glutamine (0.25% Gly-Gln group). The results showed that the diarrhea rates in 3% HWG and 5% HWG groups were significantly lower than in control group from d 1 to 14 (P＜0.05), while the average daily gain (ADG) in each of two groups was increased (P＞0.05). When compared with control group, dietary supplementation with 3% HWG increased the ratio of CD4+:CD8+ and the ratio of serum albumin and globulin concentrations (A:G) on d 14 and 28, as well as the proliferation of T- and B-lymphocytes (P＞0.05) on d 28. In addition, on d 14 and 28, the A:G ratio in 5% HWG group was significantly higher than in control group (P＜0.05), while the ratio of CD4+:CD8+ increased slightly (P＞0.05). Interestingly, 0.25% Gly-Gln group had higher proportion of CD3+ (P＞0.05) and CD4+ (P＜0.05) on d 14 than control group, but growth performances of 0.25% Gly-Gln group were negatively affected at all experiment stages. These results suggested that HWG might improve the growth performance of piglets by strengthening cell immunity and decreasing the occurrence of diarrhea during the prophase after weaning.     

逍遥津公园的改造研究

通过分析逍遥津公园面临的问题,探讨了对逍遥津公园进行保护与更新改造的可行性方法,力求证实通过这项改造将使此地块在交通流量、业态功能、地区形象及商业价值中均能取得长足发展,为今后的城市旧公园改造设计提供参考性建议。     

日粮阴阳离子水平对围产期奶水牛泌乳性能的影响

本试验研究了日粮阴阳离子(DCAD)水平对围产期的泌乳水牛采食量、尿液pH值、泌乳初期泌乳性能的影响。选择32头2胎以上、品种、上期泌乳量、健康体况相似的处于围产期的泌乳水牛,随机分成4个组,分别饲喂DCAD水平为+139.89、+54.22、-29.11和-110.21mmoL/kg的4种日粮进行试验,试验期为产前21 d开始至产后30 d。结果表明:随着日粮DCAD水平的降低,各组间试验水牛干物质采食量无显著差异,尿液pH值随之显著降低。各组产奶量无显著差异,但可提高水牛产后泌乳量2.50%～4.86%,极显著降低水牛乳体细胞数(P<0.01);乳蛋白率、乳脂率、乳糖率均有显著提高(P<0.05)。综上所述,泌乳水牛日粮适宜DCAD水平为-110.21 mmoL/kg左右。     

B allele in Iα1 hs1,2 VNTR region is associated with IgA nephropathy

AIM: To investigate the relationships between Iα1 hs1,2 VNTR polymorphism and IgA nephropathy. METHODS: Four hundred and ninteen patients with IgA nephropathy and their first-degree relatives were recruited. Two hundred and one sex and age-matched normal Chinese Han volunteers were also recruited as controls. After extracting genomic DNA, the VNTR genotypes of Iα1 hs1,2 region were determined by PCR and electrophoresis, and the results were analyzed by transmission disequilibrium test (TDT) and haplotype relative risk (HRR) in the families, and Chi-Square test in the case-control analysis. RESULTS: ① TDT analyses showed that B allele of the Iα1 hs1,2 VNTR region was significantly more transmitted from heterozygous parents to patients than expected (101 Trios, χ2=6.818, P＜0.01), extended TDT produced the same results (164 families, χ2=7.583, P＜0.01). ② Consistent with the TDT results, HRR also showed that B allele was over-transmitted to patients (P＜0.05, χ2=4.122, HRR=1.180), and the BB genotype conferred a higher risk of developing the disease (P＜0.05, χ2=4.411, OR=1.538). ③ The case-control study indicated that the B allele had a higher frequency in the IgA nephropathy group (χ2=6.968, P＜0.05). CONCLUSION: B allele in Iα1 hs1,2 VNTR region is associated with susceptibility to IgA nephropathy.     

鸡缩胆囊素(CCK)在T4噬菌体表面的展示及免疫原性研究

采用T4噬菌体表面展示系统，将CCK-33肽四多串联体及八多串联体展示在T4噬菌体表面，并研究其免疫原性。将CCK四串联体和八串联体与SOC基因缺失的、依赖溶菌酶的噬菌体ФT4-Z1重组，获得重组噬菌体CT-4CCK和西T-8CCK。SDS-PAGE和Western Blot检测结果表明，CCK多串联体蛋白已经成功展示在T4噬菌体表面，其融合蛋白分子量约30ku和41ku，能够与CCK标准阳性血清发生特异性反应。以重组噬菌体为免疫原制备油佐剂疫苗主动免疫胡须肉鸡后，抗体浓度显著升高，肉鸡的体重、采食量都有所提高，而料肉比有所下降。结果说明，鸡CCK蛋白展示在T4噬菌体表面具有良好的免疫原性。     

本地毛形线虫HSP70基因的克隆与序列分析

根据GenBank中已发表的布氏旋毛虫HSP70基因序列设计了1对引物，用Trizol法从本地毛形线虫肌幼虫虫体中提取总RNA，用RT-PCR方法扩增了HSP70基因，将目的基因克隆至pMD18-T载体，转化大肠杆菌DH5α。重组质粒用PCR、限制性内切酶BarnHⅠ和HindⅢ进行单、双酶切鉴定。测序结果表明，成功克隆了本地毛形线虫HSP70基因。序列分析表明，HSP70基因比较保守，不同物种之间氨基酸的同源性在40％～80％。与布氏旋毛虫HSP70序列相比，CDS区多出9个碱基，但核苷酸序列和氨基酸同源性分别为98%和94％，存在1个糖基化位点和1个潜在的信号肽位点。     

樟树叶水浸液对钉螺过氧化物酶等的影响

用1%~0.1%浓度梯度的新鲜樟树叶的水浸液处理血吸虫的唯一中间寄主———钉螺,设清水饲养为对照。结果表明:樟树叶水浸液处理4~5d后可达到100%的杀灭钉螺效果。采用聚丙稀酰胺电泳技术分离经浓度为1%~0.1%的樟树新鲜叶水浸液处理钉螺样品的过氧化物酶同工酶,经处理24h时样品的酶活高于对照组,而处理48h样品的酶活则大大减弱。由此在微观领域探究了樟树化感作用导致钉螺的病理变化等方面所表现出的杀伤钉螺的机理,获得强他感作用植物樟树灭螺的证据。     

基因芯片及其在病原微生物检测中的应用

基因芯片是近年来迅速发展的一门生物高新技术,它以其能够快速、高效、大规模地同步检测生物遗传信息的卓越功能而得到发展。在基因测序、基因表达分析、药物筛选、基因诊断等领域显示出重要的理论和实用价值。作者就基因芯片技术的原理、技术要点以及在病原微生物检测中的应用作一综述。