Efficacy of omeprazole paste for prevention of gastric ulcers in horses in race training

OBJECTIVE: To determine the minimal effective dosage of omeprazole oral paste for the prevention of naturally occurring ulcers in horses starting race training. DESIGN: Prospective study. ANIMALS: 175 horses. PROCEDURE: Horses in the dose selection portion of the study were sham dose treated or received 1 mg (0.45 mg/lb) or 2 mg (0.9 mg/lb) of omeprazole/kg, PO, every 24 hours for 28 days or 4 mg of omeprazole/kg (1.8 mg/lb; loading dose), PO, every 24 hours for 4 days, then 1 or 2 mg of omeprazole/kg, PO, every 24 hours for 24 days. Horses in the dose confirmation portion of the study were sham dose treated or received 1 mg of omeprazole/kg, PO, every 24 hours for 28 days. Gastric ulcer scores at the beginning and end of the study were compared. RESULTS: Sham-dose-treated horses had significantly higher ulcer scores than did horses treated with any of the omeprazole dosages evaluated. Among horses treated with omeprazole, there was no significant interaction of dose (1 or 2 mg/kg) and loading dose; therefore, the lowest effective dose (1 mg/kg) was evaluated in the dose confirmation portion of the study. In the dose confirmation study, 4 of 39 (10%) sham-dose-treated horses remained ulcer free, which was significantly different from the proportion of horses (31/38 [82%]) receiving 1 mg of omeprazole/kg that remained ulcer free. CONCLUSIONS AND CLINICAL RELEVANCE; Results indicated that omeprazole administered at a dosage of 1 mg/kg, PO, every 24 hours for 28 days was effective for prevention of gastric ulcers in horses starting race training.     

Efficacy of omeprazole paste for prevention of recurrence of gastric ulcers in horses in race training

OBJECTIVE: To determine whether omeprazole oral paste administered at a dosage of 0.5 or 1 mg/kg (0.23 or 0.45 mg/lb), PO, every 24 hours would effectively prevent the recurrence of gastric ulcers in horses in race training. DESIGN: Prospective study. ANIMALS: 135 horses. PROCEDURES: Horses with gastric ulcers were treated with omeprazole at a dosage of 4 mg/kg (1.8 mg/lb), PO, every 24 hours for 28 days. Horses in the dose selection portion of the study were sham dose treated or received 0.5 or 1 mg of omeprazole/kg, PO, every 24 hours for an additional 28 days. Horses in the dose confirmation portion of the study were sham dose treated or received 1 mg of omeprazole/kg, PO, every 24 hours for an additional 28 days. Gastric ulcers were scored before and after the preventive phase of the study (day 28 to day 56) via gastroscopy, and ulcer scores were compared. RESULTS: Sham-dose-treated horses and horses receiving 0.5 mg of omeprazole/kg had significantly higher ulcer scores than did horses receiving 1 mg of omeprazole/kg. There was a significant difference between the proportion of horses receiving 1 mg of omeprazole/kg (38/48 179%]) that remained ulcer free and the proportion of sham-dose-treated horses (7/44 [16%]) that remained ulcer free. CONCLUSIONS AND CLINICAL RELEVANCE: Omeprazole oral paste administered at a dosage of 1 mg/kg, PO, every 24 hours for 28 days was effective for prevention of recurrence of gastric ulcers in horses in race training.     

Comparative antiviral and proviral factors in semen and vaccines for preventing viral dissemination from the male reproductive tract and semen

Many animal and human viruses are disseminated via semen, but there is little information on how to measure and stimulate protective antiviral immunity in the male reproductive tract and semen. This information is important since successful vaccination through the stimulation of protective immune responses could be a mechanism to prevent viral contamination of semen and subsequent wide spread viral dissemination. Even control of the infection by shortening the duration of viral shedding and lowering the viral load in semen would lessen the chances of viral dissemination through this route. This review will highlight the current knowledge of immunity in the male reproductive tract and summarize 'antiviral' as well as 'proviral' factors in semen such as cytokines, cells, antibodies, antimicrobial peptides, enzymes, hormones and growth factors. These factors must provide a fine balance between 'immunosuppression' in semen needed to protect sperm viability and 'immunocompetency' to prevent pathogen contamination. The review will also suggest continuing challenges to researchers for preventing viral dissemination via semen and propose a large animal model for continued research in this important area.     

Effects of N–P–K deficiency and temperature regime on the growth and development of Lilium longiflorum ‘Nellie White’ during bulb production under phytotron conditions

One-year-old scale bulblets of Lilium longiflorum Thunb. ‘Nellie White’ (Easter lily) were grown under a combination of six constant day/night temperature regimes and five N–P–K nutrient treatments under short days for 107 d (growing period 1 or GP-1) to compare the effects on growth and development and bulb production. Results during GP-1 were as follows: failure of bulblets to produce a shoot (“no-shows”) was found at high temperatures (30/26 and 26/22 °C) and not influenced by the nutrient treatments. Flower bud abortion was observed in the minus-N, minus-P, and minus-N–P–K treatments at high temperatures (30/26 or 26/22 °C), but not observed at any temperatures in the complete and minus-K treatments. The loss of bulb fresh weight in minus-N treated bulblets was less than in the other treatments resulting in less root and shoot growth in the minus-N treatment. At the intermediate temperatures where growth was highest, omission of N, P, K, or all three resulted in losses in stem bulb fresh weight, stem plus leaf fresh weight, number of flowers, and stem root fresh weight. Omission of N, P, or all three nutrients resulted in lowest basal root fresh weight. Bulb N and K concentrations were lowest in plants grown with complete nutrient solution at the two coldest temperature regimes (14/10 and 10/6 °C). Bulb P concentration was lowest at the three coldest (18/14, 14/10 and 10/6 °C) and the warmest (30/26 °C) temperature regimes. Stem length was shorter when P was omitted. Omission of any of the three nutrients resulted in lower concentrations of the other nutrients. The one exception was where low K did not affect N concentration. In the second phase of the experiment, plants grown at 18/14 °C and irrigated with the complete nutrient solution for 107 d (GP-1) were continued at this day/night temperature regime and five N–P–K nutrient treatments for another 89 d under long days (growing period 2 or GP-2). Results during GP-2 were as follows. Basal bulb yield was not impacted by omission of N, P, or K, or all three. Of all growth measurements, only stem plus leaf fresh weight was lower and only when all three nutrients (minus-N–P–K) were omitted. At the end of GP-2, basal bulb concentrations of N and P did not differ from the concentrations in bulbs at the beginning of GP-1; however, K concentration was lower at the end of GP-2. Omission of N or P further resulted in lower bulb K concentration, suggesting that a moderate supply of N, P, and K be applied during GP-2 since an additional year of bulb production is needed to produce forcing-sized bulbs.     

Area utilization of gulls in a coastal farmland landscape: habitat mosaic supports niche segregation of opportunistic species

The intensively farmed coastal lowland landscape of Germany, adjacent to the North Sea, provides important foraging opportunities for Black-headed, Common, Herring and Lesser Black-backed gull (Larus ridibundus, L. canus, L. argentatus and L. fuscus). We expected that spatial and temporal utilization of the landscape mosaic as well as behavioural traits and utilization of food resources would differ between these closely related species, facilitating niche segregation. We recorded habitat types and their utilization by the four species over a whole year. Furthermore, we related species abundance to several abiotic parameters. Black-headed and Common gulls were the most numerous species in the study area throughout the year. In general, the former species preferred bare fields with recently prepared soils and was often associated with tractors in the fields, whereas the latter species was most often found on pastures. Black-headed gulls seem to have a higher ability to exploit ephemeral, food sources associated with human activities whereas common gulls prefer habitats with low human activity and with naturally distributed prey. The most prominent abiotic parameter influencing gull abundance was presence of tractors. Black-headed gulls have most likely benefited from recent changes in agricultural practice, particularly the increase in cropped land, while Common gulls may have suffered from a decline in pastures. At present, utilization of the farmland habitat mosaic leads to niche segregation and supports coexistence, as two of the four gull species mainly forage in the marine environment, while there is significant habitat partitioning between the other two temporally, spatially and behaviourally.     

Nitrogen fertilization during planting and establishment of the urban forest: A collection of five studies

Today's urban forest increasingly consists of planted trees, especially as native forest fragments yield to urban sprawl. These trees are usually larger (over 2-m tall) than typical reforestation trees and grow very little for the first few years after planting. Stressful urban sites exacerbate this effect and many practitioners hope to shorten the time required to reach environmentally functional size by fertilizing at planting. This is a controversial practice since nitrogen (N) application creates the potential for water quality impairment and effectiveness is uncertain. It is not clear how nitrogen application affects large trees with radically altered root:shoot ratios or how nursery production methods and restrictive sites affect response. In a series of five separate studies, we tested several N rates on ten shade tree species (both field- and container-grown) and transplanted to a range of urban sites, from a relatively undisturbed forest fragment to a highly compacted cutover soil with an absent A horizon. Trunk diameter increase, as an integrative metric of tree biomass accumulation, was followed for up to 4 years on each experiment. Overall, we saw little effect from fertilizing at planting at any rate we tested, regardless of location. Three studies that included leaf analysis with a SPAD-502 chlorophyll meter indicated that neither SPAD meter values or N concentration within leaves was increased by fertilizing at planting, suggesting that the newly planted shade trees took up very little of the applied N. Overall, SPAD-502 readings correlated well with actual leaf N concentration (r=0.692). This group of studies indicates that fertilization at planting does not increase post-transplant growth, even in stressful urban sites and it is therefore not effective at shortening the establishment period of transplanted shade trees.     

Evaluation of urine specific gravity and urine sediment as risk factors for urinary tract infections in cats

Background: It has been suggested that diseases that promote isosthenuria predispose to urinary tract infections because of a lack of the common bacteriostatic properties present in concentrated urine. Objectives: The purpose of this study was to assess the clinicopathologic risk factors for positive urine culture outcome in cats with chronic kidney disease (CKD), diabetes mellitus (DM), uncontrolled hyperthyroidism (HT), or lower urinary tract disease (LUTD). Methods: For this retrospective study, medical records of all cats in which a urinalysis and aerobic bacterial urine culture were performed between January 1995 and December 2002 were reviewed. Signalment, body weight, and clinicopathologic data were recorded. Based on the medical records, cats were diagnosed with CKD, DM, HT, or LUTD. Prevalence odds ratios and 95% confidence intervals were calculated using logistic regression. Multivariate models were created for each variable of interest while controlling for the confounding effect of disease group. Results: Six hundred fourteen cats met the criteria for inclusion in the study. Overall, positive urine cultures were identified in 16.9% of cats with CKD, 13.2% of cats with DM, 21.7% of cats with HT, and 4.9% of cats with clinical signs of LUTD. Decreasing urine specific gravity was not associated with positive urine culture when controlled for disease but pyuria, bacteriuria, and hematuria were all associated with positive urine culture outcome. Persians, females, increasing age, and decreasing body weight were all associated with positive urine culture outcome. Conclusions: Performing a urine culture sample based solely on the presence of isosthenuria does not seem warranted. Further studies are warranted to help identify host predisposing factors for urinary bacterial colonization in cats with these diseases.