Resistance selection and biochemical characterization of spinosad resistance in Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)

A Helicoverpa armigera population was collected from Shandong province, China. After 15 generations of selection in the laboratory, the H. armigera strain developed more than 20-fold resistance to spinosad. At LD₅₀ level, no significant cross-resistance was found between spinosad and chlorpyrifos, methomyl, avermectin and chlorfenapyr except for fenvalerate with a low cross-resistance of 2.4-fold. However, LD₉₉ values of fenvalerate against the parental and resistant strains were not different significantly. After inhibitors were used, spinosad resistance could be partially suppressed by piperonylbutoxide (PBO) and triphenylphosphate (TPP), but not by diethylmaleate (DEM). Activities of p-nitroanisole O-demethylase (ODM) developed to 8.26-fold compared with the parental strain, but no obvious changes were found in activities of carboxyl esterase (CarE) and glutathione-S-transferase (GST). The results indicated that resistance to spinosad in the cotton bollworm might be associated with an increase in cytochrome P450 monooxygenase.     

土壤水分含量与施肥量对辣椒产量与品质的影响

通过盆栽大方线辣椒,研究了土壤水分含量与施氮量对辣椒果实产量和品质的影响。结果表明,土壤水分含量和施氮量对产量影响显著,其中W2N2(土壤水分下限为60%Q,0.2gN/kg干土)产量最高;同一土壤水分含量,施氮能显著的增加辣椒素、维生素C、干物质和可溶性糖等物质含量,但施氮过多,辣椒素、维生素C含量下降;同一施氮量,土壤水分含量下降辣椒素、维生素C含量显著增加。综合来看,W2N2产量最高,品质较好。     

The frontier of soil science: Soil health

<正>Soils are a valuable resource with life activity in terrestrial ecosystem, and soil health and its sustainable management are becoming a major focus of global concern. A healthy soil is a “harmonious social system”, which should have good structure, functional state,and buffering performance to maintain the dynamic balance of soil ecosystem. Soil health has become the frontier of soil science. The development of theoretical and practical approaches for soil health evaluation and managem...     

沙土冬闲田种植青饲黑麦的可行性分析

在对沙地种植黑麦的方式，利用效益及增产技术进行研究的基础上，对沙土冬闲地的利用现状，存在问题及种植青饲黑麦的实际效益与可行性进行了分析，以期为其推广应用提供依据。     

河南省部分地区鸡源致病性大肠杆菌生物学特性的研究

从河南省不同发病鸡群中采取典型鸡大肠杆菌病痛料，分离鉴定出8株鸡大肠杆茵。8株分离茵对雏鸡具有不同程度的致病作用，其中以兰考株和南阳株致病力最强，可使接种雏鸡100％发病死亡，其次是西华株和登封株，致死率均达75％。药敏试验结果表明：8个茵株均对氟苯尼考、头孢噻呋、头孢曲松、头孢唑啉、阿米卡星、卡那霉素、氯霉素高度敏感；大多数茵株对氧氟沙星、环丙沙星、氨苄西林、链霉素严重耐药。O因子血清凝集试验结果表明：8个茵株的血清型，大多数为O78，占75％，少数为O119占25％。     

滇南红厚壳的造林技术研究

滇南红厚壳(Calophyllum polyanthum)是藤黄科(Guttifereae)高大乔木，高30～35m，胸径达1．2m。树冠在林中或散生时均呈塔形，树干通直圆满，自然整枝良好，枝下高高，出材率高；木材纹理直，结构较细且均匀，木质软硬适中、强度中等，锯解和旋刨性能极佳，表面平滑光洁，易干燥，不开裂，少变形，油漆后光亮红润，胶     

Safe conservation and utilization of peanut germplasm resources in the Oil Crops Middle-term Genebank of China

Peanut(Arachis hypogaea L.)is an important oil and cash crop in the world.Peanut germplasm collected in China are abundant,which provides important material gua...     

Apoptosis‐Like Events and In Vitro Fertilization Capacity of Sex‐Sorted Bovine Sperm

This study utilized three staining assays (Annexin V, mitochondrial membrane potential (JC‐1) and TUNEL) for flow cytometric analysis of apoptosis in sex‐sorted sperm from four different bulls (A, B, C and D). Correlations between sperm quality and IVF efficiency were then assessed to determine which assay provided the best prediction of IVF efficiency. The results of the Annexin V assays, as well as measures of viable sperm, early apoptosis, necrotic sperm and mitochondrial membrane potential (?ψm) showed that the sex‐sorted sperm collected from bull A significantly differed from those of the other three bulls (p < 0.05). In addition, the levels of DNA fragmentation in sex‐sorted sperm from bull A were significantly lower than those from bulls B and C (p < 0.05). The percentage of cells reaching the cleavage and blastocyst stages in sex‐sorted sperm from bull A were significantly greater than those from the other bulls (p < 0.05). A significant positive correlation was observed between viable sperm and the percentage of cells at the cleavage or blastocyst stages (p < 0.05). In contrast, a negative correlation was found between early apoptotic sperm and the percentage of cells at the cleavage or blastocyst stages (p < 0.05). In conclusion, these results indicated that the Annexin V assay was the most reliable technique for the prediction of the IVF success of sex‐sorted bovine sperm.