Simultaneous quantification of ginsenosides in American ginseng (Panax quinquefolium) root powder by visible/near-infrared reflectance spectroscopy.

Near-infrared reflectance spectroscopy (NIRS) was examined as a possible alternative to high-performance liquid chromatography (HPLC) for the analysis of ginsenosides from American ginseng (Panax quinquefolium) root powder (n = 26). NIR spectra were collected over 400-2500 nm. For each sample and individual ginsenoside quantified by HPLC, spectral data were regressed against the chemical data to develop prediction equations. The spectral prediction equations produced high correlation coefficient (1-VR) values and low standard errors of cross validation (SECV) values for the determination of individual and total ginsenosides. The contents of individual ginsenosides, Rb(1), Rb(2), Rc, Rd, Re, Rg(1), Ro, m-Rb(1), m-Rb(2), m-Rc, m-Rd, and total ginsenosides (X +/- SECV) were (1.29+/-0.18)%, (0.273+/-0.096)%, (0.298+/-0.052)%, (0.091+/-0.027)%, (1. 015+/-0.114)%, (0.116+/-0.018)%, (0.25+/-0.040)%, (0.776+/-0.116)%, (0.197+/-0.074)%, (0.239+/-0.083)%, (0.143+/-0.042)%, and (4.393+/-0.283)%, respectively. The (1-VR) values of cross validation were 0.877, 0.872, 0.955, 0.834, 0.899, 0.919, 0.325, 0.849, 0.902, 0.877, 0.871, and 0.963, respectively. Results indicated that the NIRS method could be used for the analysis of the major ginsenosides, Rb(1), Re, and m-Rb(1), as well as the total ginsenosides in American ginseng.     

Comparison of mass spectrometry-based electronic nose and solid phase microextraction gas chromatography-mass spectrometry technique to assess infant formula oxidation

Two headspace techniques based on mass spectrometry detection (MS), electronic nose, and solid phase microextraction coupled to gas chromatography-mass spectrometry (SPME-GC/MS) were evaluated for their ability to differentiate various infant formula powders based on changes of their volatiles upon storage. The electronic nose gave unresolved MS fingerprints of the samples gas phases that were further submitted to principal component analysis (PCA). Such direct MS recording combined to multivariate treatment enabled a rapid differentiation of the infant formulas over a 4 week storage test. Although MS-based electronic nose advantages are its easy-to-use aspect and its meaningful data interpretation obtained with a high throughput (100 samples per 24 h), its greatest disadvantage is that the present compounds could not be identified and quantified. For these reasons, a SPME-GC/MS measurement was also investigated. This technique allowed the identification of saturated aldehydes as the main volatiles present in the headspace of infant milk powders. An isotope dilution assay was further developed to quantitate hexanal as a potential indicator of infant milk powder oxidation. Thus, hexanal content was found to vary from roughly 500 and 3500 microg/kg for relatively non-oxidized and oxidized infant formulas, respectively.     

翻转卸料双轴桨叶饲料混合机工作性能试验研究

该文介绍了作者设计并研制的新型翻转式双轴桨叶混合机,阐述了其工作原理。为了提高双轴桨叶混合机的混合性能,采用二次回归正交旋转组合试验,对设计样机进行混合均匀度及机内残留率的试验研究,得出了其工作参数的合理组合：混合时间为180 s,充满系数为0.75,桨叶轴转速为56 r/min;机内残留量极小(约为0.015%),满足其作为配合饲料生产,尤其是预混合饲料生产的工艺要求,也为进一步改进设计提供了依据。     

辽宁省东部山区5种森林植被类型水文生态效益综合评判

利用层次分析与Fuzzy数学相结合方法,对辽宁省东部山区5种森林植被类型水源涵养能力进行了多目标综合评判。首先利用层次分析法确定了评价因子林冠截留、林冠蒸散、枯落物蓄水、土壤容重、非毛管孔隙度、初渗速率、稳渗速率、土壤总蓄水和土壤有效水的权重集为C=(0.0245,0.0051,0.1993,0.0109,0.0762,0.0565,0.2827,0.0573,0.2866),然后利用最大模糊熵原则确定了评价因子的隶属函数,最后利用Fuzzy综合评判得出油松、落叶松、红松、柞木林、杂木林评价结果模糊子集为B=(0.4686,0.3784,0.4145,0.6128,0.4808),结果表明阔叶林水源涵养效益高于针叶林,其中柞木林效益最佳。     

Molecular characterization and PCR detection of a nitrogen-fixing Pseudomonas strain promoting rice growth

Nitrogen-fixing plant growth-promoting rhizobacteria (PGPR) from the genus Pseudomonas have received little attention so far. In the present study, a nitrogen-fixing phytohormone-producing bacterial isolate from kallar grass (strain K1) was identified as Pseudomonas sp. by rrs (16S ribosomal RNA gene) sequence analysis. rrs identity level was high with an uncharacterized marine bacterium (99%), Pseudomonas sp. PCP2 (98%), uncultured bacteria (98%), and Pseudomonas alcaligenes (97%). Partial nifH gene amplified from strain K1 showed 93% and 91% sequence similarities to those of Azotobacter chroococcum and Pseudomonas stutzeri, respectively. The effect of Pseudomonas strain K1 on rice varieties Super Basmati and Basmati 385 was compared with those of three non-Pseudomonas nitrogen-fixing PGPR (Azospirillum brasilense strain Wb3, Azospirillum lipoferum strain N4 and Zoogloea strain Ky1) used as single-strain inoculants. Pseudomonas sp. K1 was detected in the rhizosphere of inoculated plants by enrichment culture in nitrogen-free growth medium, which was followed by observation under the microscope as well as by PCR using a rrs-specific primer. For both rice varieties, an increase in shoot biomass and/or grain yield over that of noninoculated control plants was recorded in each inoculated treatment. The effect of Pseudomonas strain K1 on grain yield was comparable to those of A. brasilense Wb3 and Zoogloea sp. Ky1 for both rice varieties. These results show that nitrogen-fixing pseudomonads deserve attention as potential PGPR inoculants for rice.     

Genotypic variation in grain cadmium concentration of lowland rice

Cadmium (Cd) contamination of paddy rice soils is commonly observed in the Yangtse River Delta, China. Large Cd uptake by rice plants and its translocation into the grains can entail human‐health risks. Genotypic variations in Cd uptake and a differential Cd partitioning into grains will be the basis for developing a rice screening or breeding tool for low grain Cd. A field experiment, conducted at the experimental farm of Jiaxing, Zhejiang province from 2002 to 2004, compared 38 rice genotypes of different types (indica vs. japonica) collected from the Yangtse River Delta. The results showed large differences in Cd concentrations in straw, brown rice, and grain chaff among the rice genotypes grown on Cd‐contaminated soil. Concentrations in brown rice ranged from 0.06 to 0.99 mg Cd kg^–1. The total Cd uptake in brown rice varied between 0.96 and 28.58 μg plant ^{– 1}. In general, indica‐type cultivars accumulated significantly more Cd than the japonica‐type cultivars. The Cd concentration in straw was highly correlated with that in brown rice. While significant differences in the Cd‐partitioning ratio (% share of total Cd uptake found in brown rice) among rice genotypes were observed, these were not correlated with Cd concentration of brown rice. This indicates that the Cd accumulation in rice grains appears to be governed mainly by the Cd uptake by the plant and probably not by differential Cd partitioning. The large genotypic variation suggests the possibility to lower the Cd content of rice by genotype selection. The development of such breeding tools should focus on low Cd uptake rather than Cd partitioning between straw and grain.     

长期定位施肥对无石灰性潮土酶活性的影响

试验研究长期定位施肥对无石灰性潮土酶活性的影响结果表明,长期单施有机肥及其配施无机化肥均能显著增强土壤脲酶、蛋白酶、磷酸酶和蔗糖酶的活性。土壤脲酶活性、蛋白酶活性与土壤有机质、全N、碱解氮含量呈极显著正相关,磷酸酶活性与土壤有机质、有机磷、有效磷呈极显著正相关,蔗糖酶活性与土壤有机质呈极显著正相关。表明土壤酶活性与土壤肥力密切相关,可作为评价土壤肥力性状的生物学指标。     

基于激光图像的苹果品质分析与模型

该试验利用波长650 nm，功率25 mW的半导体激光及计算机视觉技术，初步探讨了激光图像的影响因素以及利用激光图像对苹果(嘎拉、红富士)品质进行无损检测的可行性。分别测定贮藏期间苹果的硬度、固酸比、果面底色、果型等品质指标，并获取图像参数及其像素个数(S1、S2、S3、S4)。试验结果显示，苹果的向阳面和背阳面对获取的激光图像的像素个数有显著的影响，而果型大小对其无显著影响。其中图像参数S3与各品质指标的皮尔逊相关系数最高。逐步回归表明，引入变量S3后模型的解释率最好(最高R²为0.99)，且都为极显著水平(P≤0.01)。试验的结果为利用小功率激光图像对苹果品质进行无损检测提供了理论依据。     

广东惠阳水稻种植中耕牛与小型拖拉机使用的能值比较

以能值理论方法为手段，从资源的投入和输出结构角度，对广东省惠州市惠阳区典型水稻种植户的耕牛和小型拖拉机这两种典型的农业驱动力产出系统的转化与生产效率，环境负载和可持续性进行比较分析与评价。研究表明：耕牛系统在单位面积水稻田上消耗的不可更新资源量是拖拉机系统的4.57倍；人力能值消耗是拖拉机系统的6.14倍。相对于拖拉机动力，耕牛产出动力处于较低的能量等级，其环境负载率(ELR)仅是拖拉机系统的24%，可持续能值指标(ESI)是拖拉机系统的4.25倍，但有巨大的优化空间和可能。而广东小型拖拉机系统产出驱动力的使用效率则稍高于典型65 kW拖拉机系统。从提高能值效率角度分析，减少耕牛系统生产过程中过多的人力投入，着力提高耕牛系统产出驱动力的利用效率比发展小型拖拉机系统更有潜力。生产过程中的人力投入和使用过程中的维护投入是农机驱动力产出系统不可忽视的能值投入项。     

Capillary electrophoresis and high-performance liquid chromatography determination of polyglutamyl 5-methyltetrahydrofolate forms in citrus products

The 5-methyltetrahydrofolate (5mTHF) polyglutamates in citrus products were analyzed by capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC). Folate species were purified from citrus products and concentrated from 2- to 100-fold using combined folate-affinity chromatography and C18 extraction. Seven polyglutamyl 5mTHFs were found in most not-from-concentrate (NFC) orange juices (OJ) in total amounts of approximately 1 nmol/mL, with varying distributions of individual polyglutamates. Folate amounts and distributions were also measured in orange fractions, single-strength OJ from concentrate, NFC grapefruit juice, and citrus peel molasses. Models containing ascorbic acid had folate thermal degradation rates one-seventh that of models without ascorbic acid. Pasteurization studies demonstrated that folate loss was <2% for commercial OJ pasteurization conditions (i.e., 93 degrees C for 5 s, 88 degrees C for 15 s, and 82 degrees C for 30 s). Both methods were precise, reproducible, and potentially faster than traditional analytical procedures requiring enzymatic deconjugation and microbial assays.