Cyanobacteria mediated plant growth promotion and bioprotection against Fusarium wilt in tomato

Cyanobacteria - phytopathogenic fungi - tomato plant interactions were evaluated for developing suitable biological options for combating biotic stress (Fusarium wilt) and enhancing plant vigour. Preliminary evaluation was undertaken on the fungicidal and hydrolytic enzyme activity of the cyanobacterial strains (Anabaena variabilis RPAN59, A. laxa RPAN8) under optimized environmental/nutritional conditions, followed by amendment in compost-vermiculite. Such formulations were tested against Fusarium wilt challenged tomato plants, and the Anabaena spp. (RPAN59/8) amended composts significantly reduced mortality in fungi challenged treatments, besides fungal load in soil. Cyanobacteria amended composts also led to an enhancement in soil organic C, nitrogen fixation, besides significant improvement in growth, yield, fruit quality parameters, N, P and Zn content. The tripartite interactions also enhanced the activity of defence and pathogenesis related enzymes in tomato plants. A positive correlation (r?=?0.729 to 0.828) between P content and pathogenesis/defense enzyme activity revealed their role in enhancing the resistance of the plant through improved nutrient uptake. Light and scanning electron microscopy (SEM) revealed cyanobacterial colonization, which positively correlated with reduced fungal populations. The reduced disease severity coupled with improved plant growth/ yields, elicited by cyanobacterial treatments, illustrated the utility of such novel formulations in integrated pest and nutrient management strategies for Fusarium wilt challenged tomato crop.     

Genetic Relationship and Structure Analysis of Root Growth Angle for Improvement of Drought Avoidance in Early and Mid-Early Maturing Rice Genotypes

Deeper rooting 1(Dro1) and Deeper rooting 2(Dro2) are the QTLs that contribute considerably to root growth angle assisting in deeper rooting of rice plant. In the present study, a set of 348 genotypes were shortlisted from rice germplasm based on root angle study. Screening results of the germplasm lines under drought stress identified 25 drought tolerant donor lines based on leaf rolling, leaf drying, spikelet fertility and single plant yield. A panel containing 101 genotypes was constituted based on screening results and genotyped using Dro1 and Dro2 markers. Structure software categorized the genotypes into four sub-populations with different fixation index values for root growth angle. The clustering analysis and principal coordinate analysis could differentiate the genotypes with or without deeper rooting trait. The dendrogram constructed based on the molecular screening for deep rooting QTLs showed clear distinction between the rainfed upland cultivars and irrigated genotypes. Eleven genotypes, namely Dular, Tepiboro, Surjamukhi, Bamawpyan, N22, Dinorado, Karni, Kusuma, Bowdel, Lalsankari and Laxmikajal, possessed both the QTLs, whereas 67 genotypes possessed only Dro1. The average angle of Dro positive genotypes ranged from 82.7° to 89.7°. These genotypes possessing the deeper rooting QTLs can be taken as donor lines to be used in marker-assisted breeding programs.     

ZINC-COATED UREA IMPROVES PRODUCTIVITY AND QUALITY OF BASMATI RICE (ORYZA SATIVA L.) UNDER ZINC STRESS CONDITION

A field study conducted for two years (2006 and 2007) at the Research Farm of the Indian Agricultural Research Institute, New Delhi, India showed that zinc (Zn) fertilization increased yield attributes, grain and straw yield, enhanced Zn concentrations and its uptake and improved kernel quality before and after cooking in basmati rice ‘Pusa Sugandh 5’. A 2% Zn-coating with zinc sulfate (ZnSO₄·7H₂O) was found to be the best but a 2% Zn-coating with zinc oxide (ZnO) was very close to it in terms of grain and straw yield and Zn concentrations in basmati rice grain and straw under Zn stress conditions. Partial factor productivity (PFP) of applied Zn varied from 984–3,387 kg grain kg Zn^?1, agronomic efficiency (AE) varied from 212–311 kg grain kg^?1 Zn (applied) and physiological efficiency (PE) of Zn varied from 6,384–17,077 kg grain kg^?1 Zn (absorbed). Thus, adequate Zn fertilization of basmati rice can lead to higher grain yield and Zn-denser grains with improved cooking quality in basmati rices under Zn stress soil conditions.     

Balanced and integrated nutrient management for enhancedand economic food production: case study from rainfed semi-arid tropics in India

Soil degradation in the semi-arid tropics (SAT) is mainly responsible for low crop and water productivity. In Madhya Pradesh and Rajasthan states in India, the soil analyses of farmers’ fields revealed widespread deficiencies of S (9–96%), B (17–100%) and Zn (22–97%) along with that of P (25–92%). Soil organic C was deficient in 7–84% fields indicating specifically N deficiencies and poor soil health in general. During on-farm evaluations in rainy seasons 2010 and 2011, the soil test based addition of deficient nutrient fertilizers as balanced nutrition (BN) increased crop yields by 6–40% (benefit to cost ratios of 0.81–4.28) through enhanced rainwater use efficiency. The integrated nutrient management (INM), however, decreased the use of chemical fertilizers in BN by up to 50% through on-farm produced vermicompost and recorded yields at par or more than BN with far better benefit to cost ratios (2.26–10.2). Soybean grain S and Zn contents improved with INM. Applied S, B, Zn and vermicompost showed residual benefits as increased crop yields for succeeding three seasons. Hence, results showed INM/BN was economically beneficial for producing more food, while leading to resilience building of SAT production systems.     

On‐farm diagnosis and management of iron chlorosis in groundnut

Abstract

Iron (Fe) chlorosis is a major nutritional constraint to groundnut (Arachis hypogaea L.) productivity in many parts of the world. On‐farm research was conducted at a Fe‐chlorotic site to evaluate the performance of three genotypes (TMV‐2, ICGS‐11, and ICGV‐86031), three fertilizer practices [no fertilizer control, fanners practice (125: 200: 0 kg NPK ha^?1), recommended practice (20: 50: 30 kg NPK ha^?1)], and two Fe treatments (non‐sprayed control and foliar FeSO₄ sprays) for their effect on Fe‐chlorosis and haulm and pod yields. These treatments were tested in a strip‐split plot design with four replicates. Results revealed that TMV‐2 and ICGS‐11 were susceptible to Fe‐chlorosis and produced significantly smaller haulm and pod yield, whereas, ICGV‐8603 1 was tolerant to Fe‐chlorosis. Farmer's fertilizer practice had the highest incidence of Fe‐chlorosis. Extractable Fe and chlorophyll content in the fresh leaves were the best indices of Fe‐status and were significantly (P<0.01) correlated with visual chlorosis ratings. Foliar application of FeSO₄ (0.5 w/ v) was effective in correcting Fe‐chlorosis and increased pod yield by about 30 to 40% in susceptible genotypes. These results suggests that use of tolerant genotypes such as ICGV‐86031 or foliar application of FeSO₄ in susceptible genotypes such as TMV‐2 and ICGS‐11 in combination with recommended fertilizer levels is an effective management package for alleviating Fe‐chlorosis in groundnut.     

Feeding quality assessment of fresh maize stover by means of near-infrared spectroscopy with a new sample presentation unit

Stover quality traits are important in breeding programmes of maize cultivars. However, conventional procedures for evaluation of stover quality are expensive and thus limit the full exploitation of the available genetic variability. Our objective was to assess the potential of near‐infrared spectroscopy (NIRS) with a new sample presentation unit to determine feeding quality of maize stover. The plant material comprised maize inbreds and hybrids. The sample presentation unit was equipped with a near‐infrared diode‐array spectrometer. Coefficient of determination in cross‐validation ranged between 0.77 and 0.94 for contents of dry matter, nitrogen, neutral detergent fibre and in vitro fermentability expressed as gas volume after 24h incubation time, but it was lower for ash content. It was concluded that NIRS with the new sample presentation unit was less accurate than laboratory NIRS to predict the standard reference methods. However, the new sample presentation unit might be used as a fast and efficient technique to perform large screenings of maize stover accessions in breeding programmes.     

Folklore medicinal plants of North Andaman Islands, India

The rural folk of North Andaman, India use the traditional medicine for their primary health care. Folklore medicinal uses of 72 interesting medicinal plant species along with botanical name, local name, family, habit, part used, disease for which the drug is administrated, mode of administration are presented. These 72 plant species which provide the crude drugs pertain to 67 genera and 43 families of Magnoliophyta from tropical rainforests. These plants used to cure 40 ailments. Most remedies were taken orally, accounting for 76% of medicinal use. Most of the remedies were reported to have been from trees (55.6%) and herb (22.2%) species. The most widely sought after plant parts in the preparation of remedies in the areas are the stem bark (33.8%) and root (23.9%).     

Growing stock variation in different teak （Tectona grandis） forest stands of Mizoram,India

The growing stock assessment of three different teak forest stands （Tuirial： 500 m asl, Sairang： 200 m asl and Phunchawng： 550 m asl） was done in 2006 in Mizoram, India. Five diameter classes were arbitrarily established for knowing the volume attribute data and population structure, viz. a （10-20 cm）, b （20-30 cm）, c （30-40 cm）, d （40-50 cm）, and e （50-60 cm）. Results revealed that the density of the individuals among the studied stands varied from 280 stems/ha to 620 stems/ha. The average diameter of all the individuals ranged between 27.48 cm and 35.43 cm. Similarly, the average height was oscillated between 17.87 m and 22.24 m. The total basal area was recorded between 24.28 m2.ha-1 and 45.80 m2.ha＂l. The maximum and minimum values of total growing stock under all the diameter classes were 669.01 m3.ha-1 and 284.7 m3.ha-1, respectively. The representation of population structure of different stands explained that the perpetuation of this species was ensured for a quite long time.     

Colonization by Rhizobia of the seed and roots of legumes in relation to exudation of phenolics

A study of the colonization pattern of Rhizobium inoculated either in the soil or on to the seeds of green gram (Phaseolus aureus Roxb.), black gram (P. mungo L.) and sun hemp (Crotalaria juncea L.) revealed a significant reduction of Rhizobium in the spermosphere of sun hemp which was attributed to excretion of phenolic compounds by the seed. As the plants aged, the quantity of phenolics exuded by the roots was reduced and there was a parallel increase in the Rhizobium population in the rhizospheres of the three plant species.     

Inactivation of seed-treatment fungicides by Rhizobium

2-Methoxyethylmercury chloride (MEMC) applied to cowpea seeds with Rhizobium provided little or no control of seedling rot caused by Rhizoctonia solani; similar treatment, but without Rhizobium, gave >40% control of disease. Treating seeds with quintozene and then with Rhizobium rendered the fungicide completely ineffective against R. solani infections; disease control was >70% when seeds were treated with quintozene only, and >60% when seeds were coated first with Rhizobium and then with quintozene; treatment with quintozene+Rhizobium gave about 25% control. Simultaneous treatment with Rhizobium considerably reduced the efficacy of captafol, chloroneb, carbendazim and thiophanate-methyl. Disease control by carboxin, benomyl and thiabendazole remained unaltered in the presence of Rhizobium, but only thiabendazole gave good control. Seeds treated with MEMC and Rhizobium produced seedlings without root nodules; nodulation was also decreased by captafol and by carboxin but six other fungicides had no effect. The implications of Rhizobium-fungicides interactions are discussed in the context of fungicidal control of root diseases.