家蚕18项血液生理生化性状的杂种优势分析

测定了家蚕 3对现行品种及其杂交种的 18项血液生理生化指标 ,计算其杂种优势指数。发现 13项指标呈正负超亲优势 ,其中 :葡萄糖含量有较高的正超亲优势 ;钙、无机磷及球蛋白含量与乳酸脱氢酶活性基本没有杂种优势 ;谷丙转氨酶等 8项性状表现正杂种优势的趋势较强 ;尿酸等 4项性状则表现负超亲优势的趋势较强。按代谢类型分 :蛋白质代谢相关的 9项性状中呈现正负超亲优势的有 5～ 8项 ,占 5 5 .6 %～ 88.9% ;能量代谢相关的 6项中有 2～ 5项 ,占 33.3%～ 83.3% ;脂代谢相关的 2项中有 1项呈负超亲优势 ,占 5 0 %。表明家蚕经济性状广泛的杂种优势有其普遍的生理生化基础     

植物叶片吸附大气颗粒物的研究综述

空气中的颗粒物包含TSP、PM10和PM2.5,严重影响和威胁了城市人口的生活质量及健康。为了降低城市居民的健康风险,控制污染源刻不容缓。植物对环境的修复具有很大的潜力,这已得到了共识。现从叶片解剖结构、叶片表观形态、叶片平面几何形态以及叶片其它方面阐述了植物叶片滞尘能力差异。不同绿化树种对颗粒物的吸附净化作用不同,通过比较不同城市绿化树种对大气中颗粒物的净化能力,来探索经济高效的环境治理方法,这在当今显得尤为重要。     

金花菜新品种‘淮扬金花菜’

‘淮扬金花菜'是通过对江苏扬州、镇江等地种质资源调查并搜集整理,经多年筛选出的新品种,具有蛋白质含量高、品质好、产量高、再生性好、抗逆性强,综合性状优良的特点。粗蛋白含量29.41%,粗灰分含量7.94%,中性洗涤纤维含量21.71%,酸性洗涤纤维含量10.29%,适宜多次刈割,鲜草产量30 000 kg·hm~(-2)以上,适宜在长江中下游地区保护地或露地秋季种植。     

Down-regulated BARF1 expression induces EBV-positive gastric carcinoma cell apoptosis via activating caspase-dependent mitochondrial pathway

AIM: To investigate the effects of BARF1 down-regulation on EBV-positive gastric carcinoma cell apoptosis, and the molecular mechanisms by BARF1 silencing-mediated apoptosis. METHODS: After NUGC3 and SNU719 cells were transfected with NCsiRNA and siRNA, respectively, the protein levels of BARF1, Bcl-2, Bax, cytochrome C, caspase 3 and capase 9 were detected by Western blot, and the mRNA expression of BARF1, Bcl-2 and Bax was determined by RT-PCR. The cell viability was measured by the method of Trypan blue exclusion and the cell apoptosis was analyzed by flow cytometry analysis with Annexin V-FITC/PI staining. The expression of the apoptosis-related proteins in the cells transfected with siRNA and NCsiRNA was examined by human apoptosis antibody arrays. Mitochondrial membrane potential was determined by flow cytometry. The interaction between Apaf-1 and caspase 9 was confirmed by immunoprecipitation. RESULTS: Compared with untreated and NCsiRNA groups, BARF1 gene silencing significantly inhibited the cell viability, induced apoptosis, and reduced the mitochondrial membrane potential in the NUGC3 and SNU719 cells transfected with siRNA. BARF1 gene silencing up-regulated the expression of pro-apoptotic proteins and down-regulated the expression of anti-apoptotic proteins, and the Bcl-2/Bax ratio was significantly decreased. In BARF1 gene silencing cells, the caspase inhibitor z-VAD-fmk inhibited BARF1 silencing-mediated apoptosis, and significantly increased the levels of cleaved caspase 3 and caspase 9. The concentration of cytochrome C significantly increased as compared with NCsiRNA group, and Apaf-1 interacted with caspase 9 in the cytoplasm. CONCLUSION: BARF1 silencing induces apoptosis via the mitochondrial pathway through regulating the expression of Bcl-2 and Bax proteins in a caspase-dependent manner in the NUGC3 and SNU719 cells.     

盐胁迫条件下甜高梁幼苗的光合特性及光系统Ⅱ功能调节

通过气体交换和叶绿素荧光猝灭动力学研究了盐胁迫对甜高粱幼苗碳同化能力和光系统Ⅱ光化学效率的影响。结果表明，50和100mmol L^-1的盐（NaO）处理对叶绿素含量、相对含水量和膜质过氧化程度影响很小；200mmol L^-1 NaCl处理导致叶绿素含量和相对含水量明显下降、膜质过氧化程度增加。50mmol L^-1 NaCl处理未影响甜高粱幼苗的净光合速率；NaCl浓度大于50mmol L^-1时，净光合速率开始迅速降低；同时，气孔限制值（Ls）也减小；而且，光合能力的下降未能通过增加CO2浓度得以恢复。甜高粱幼苗的初始荧光（Fo）、最大荧光产量（Fm）和最大光化学效率（Fv/Fm）只在200mmol L^-1 NaCl处理时有较大程度的下降。此外，50mmol L^-1 NaCl胁迫也没有影响甜高粱幼苗的荧光猝灭动力学参数；当NaCl浓度大于50mmol L^-1时，光系统Ⅱ开放反应中心转化效率（Fv，F’m），光化学猝灭系数（qp）和光系统Ⅱ实际光化学效率（ΦPSⅡ）开始下降，而非光化学猝灭（NPQ）提高。因此认为，盐胁迫导致的碳同化能力的降低属于非气孔限制；碳同化能力的降低改变了甜高粱光系统Ⅱ的激发能利用和分配。100mmol L^-1盐胁迫条件下，甜高粱幼苗主要通过增加热耗散来消耗过多的激发能，而200mmol L^-1盐胁迫条件下通过减少光能吸收和增加热耗散来维持光能捕获和利用的平衡。     

Modular Design Base on Variety Theory

Variety Design not only support to short down development time, and cut down development cost, but also support to response marketing rapid, is a kind of modern design theory which apply on Mass Customization. In some way, Variety Design is a kind of design that integrates standard design and modular design, and Variety Design object is high cost and universal parts that among platform. The authors discuss the modular design role and the different between integral design, promote the method and the step of modular design, so change the low cost parts design, and give an new product based on the platform. Based on this model, they not only save the cost of develop new product, but also save time, achieve advantage which Mass produce process.     

Innovation of Construction Projects Management Based on the Five-dimension Modeling

Nowadays,the computer technology is applied more and more widely in the architecture,engineering and construction industries(AEC).This paper introduces a methodology of construction project management based on the five-dimension modeling which integrates three-dimension building models,resources and schedules by the development of virtual construction technology.The application of this method also influences several aspects of project management such as range,time,cost,resource,communication and information management.Finally,the process of 5D modeling and the management practice are presented through a real life case in Beijing which proves to be a more efficient,productive and economy project.     

1-MCP处理对桃冷藏期间品质和生理特性的影响

秦王”桃采收后分别用不同浓度1-MCP处理,然后置于(0±1)℃冷库中贮藏。结果表明:1-MCP处理对贮藏期果实的硬度、可溶性固形物含量无显著影响。经处理的果实在贮后20天果皮细胞膜透性开始异常增加。10nL/L和30nL/L1-MCP处理的果实比对照提前5天出现了呼吸高峰和乙烯释放高峰;500nL/L的处理抑制了果实的呼吸作用,与对照相比大幅度降低了乙烯峰值,在相同贮藏时间多酚氧化酶(PPO)和过氧化物酶(POD)活性也显著降低(p<0.05),表明500nL/L的1-MCP处理能有效地抑制果肉褐变,延长果实贮藏时间。     

1-MCP对果实的影响

1-MCP是一种乙烯受体抑制剂,对果实成熟起着明显的抑制作用。在阐述影响1-MCP效应的因素和1-MCP对香蕉、梨和苹果影响的同时进一步调查了1-MCP在果实贮藏中起的作用。     

3种白三叶品种萌发期对干旱胁迫的响应及抗旱性评价

用不同浓度(0、5％、10％、15％、20％、25％)的聚乙二醇6000(PEG-6000)溶液模拟干旱胁迫条件,对3种白三叶萌发期的相对发芽率、相对发芽指数、相对活力指数、抗旱指数、相对根长、相对芽长共6项指标进行研究.结果表明:1)随着干旱胁迫程度的加剧,3种白三叶种子相对发芽率、相对发芽指数、相对活力指数、抗旱指数、相对根长、相对芽长总体均呈下降趋势,且瑞文德下降幅度最大;2)5％ PEG浓度能促进vns和荷兰白种子萌发和幼苗的生长;3)采用隶属函数法对3种白三叶萌发期的抗旱性进行了综合评价,抗旱性强弱顺序为:vns＞瑞文德＞荷兰白.