大肠杆菌肠毒素STI、STII、LTB融合基因植物表达载体的构建

产肠毒素性大肠杆菌(ETEC)LT和ST是导致人和动物腹泻的主要病原菌。利用PCR方法分别从pGEM-5Zf( )-STI、K88ab(LT ,ST )质粒中扩增到了STI、STII基因,然后通过三引物PCR实现了STI、STII基因的融合,再与LTB基因融合,并置同一阅读框。LTB基因的5′位于STII基因的3′端,并在ST基因和LTB基因之间插入7个氨基酸的连接肽。将融合基因STI-STII-LTB构建到带有核基质结合区序列(MARs)的植物表达载体pBI121-MARs中,并通过冻融法导入根癌农杆菌EHA105。     

人工控制条件下的灰树花菌株筛选

对收集到的36个灰树花(Grifola frondosa)菌株在人工控制的环境条件下进行品比试验,为工厂化生产选择表现优良的菌株.共进行4轮筛选,从出菇正常的15个菌株中选择4个表现良好的菌株进行扩大栽培,对其稳定性进行研究.筛选出原基形成率高、抗逆性好的2个来自庆元的菌株(灰M良和灰M云)用于人工控制条件下生产.     

宁夏南部山区农民增收问题探讨

通过分析宁夏南部山区农民收入的现状以及制约农民增收的因素,提出了相应的对策思路。     

电子废弃物循环利用堵在哪里？

小王是个有些环保意识的人．电池回收起来攒在盒子里。旧的手机坏了一直放在家里．最近交到了移动营业厅．因为那里开通了“绿箱子”回收手机。家里的旧电脑已经闲置两年多．却不敢卖给大街上骑三轮车收废品的人。他听说有人把计算机的电路板投入酸溶液从中提炼金银等责金属．但废液会污染河流．土地。现在他能把计算机放心地交给谁呢？[编者按]     

Design,Semisynthesis, Insecticidal and Antibacterial Activities of a Series of Marine-Derived Geodin Derivatives and Their Preliminary Structure–Activity Relationships

To enhance the biological activity of the natural product geodin (1), isolated from the marine-derived fungus Aspergillus sp., a series of new ether derivatives (2–37) was designed and semisynthesized using a high-yielding one-step reaction. In addition, the insecticidal and antibacterial activities of all geodin congeners were evaluated systematically. Most of these derivatives showed better insecticidal activities against Helicoverpa armigera Hübner than 1. In particular, 15 showed potent insecticidal activity with an IC₅₀ value of 89 μM, comparable to the positive control azadirachtin (IC₅₀ = 70 μM). Additionally, 5, 12, 13, 16, 30 and 33 showed strong antibacterial activity against Staphylococcus aureus and Aeromonas salmonicida with MIC values in the range of 1.15–4.93 μM. The preliminary structure–activity relationships indicated that the introduction of halogenated benzyl especially fluorobenzyl, into 1 and substitution of 4-OH could be key factors in increasing the insecticidal and antibacterial activities of geodin.     

Optimizing Rank of Landscape Planning Works of Urban Wetland Park

Classifying and ranking the huge amounts of landscape planning works of urban wetland park is always difficult due to the multi-functions (ecological, leisure, educational and disaster prevention) of the urban wetland park. Therefore, an optimizing rank system is urgently needed. Analytic Hierarchy Process (AHP) and Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) models were used to rank the planning works of 30 urban wetland park based on four mainly factors, which included landscape ecological planning, landscape planning, ecological planning and economic planning. The study indicated that the AHP- TOPSIS model had good discrimination in the classification and ranking of landscape planning works of urban wetland park and it was also applicable to the planning works of other urban greenbelts.     

放养密度和投饲率对南美白对虾单产的影响

为了提高养殖南美白对虾的单产水平,采用梯度设计法和回归分析法研究了放养密度和日投饲率对养殖南美白对虾单产的影响.结果 表明,新疆北疆地区南美白对虾单产(Y)与放养密度(SD)呈极显著二次曲线关系Y=-0.0461SD2+ 11.204SD-78.959(R2=0.9707)(F＞Fn01),当地南美白对虾最佳放养密度为...     

国内外养殖水产品质量安全管理体系建设现状及比较分析

对中国现行养殖水产品质量安全管理体系进行归纳与分析,同时选择日本、美国、挪威、泰国等在水产品质量管理体系建设方面具有代表性的国家,对其养殖水产品质量安全管理机构、行政管理体系、法律法规标准体系等方面进行介绍与评述,总结这些国家养殖水产品质量安全管理的发展概况和主要特点,并与中国的现行体系进行对比分析。结论认为,要确保水产品质量安全,实现政府的有力监管,进一步增强出口水产品的国际竞争力,关键是在于拥有完善的监督管理模式和先进的水产品质量安全监管体系。