Schmallenberg virus—Two years of experiences

In autumn 2011, a novel species of the genus Orthobunyavirus of the Simbu serogroup was discovered close to the German/Dutch border and named Schmallenberg virus (SBV). Since then, SBV has caused a large epidemic in European livestock. Like other viruses of the Simbu serogroup, SBV is transmitted by insect vectors. Adult ruminants may show a mild transient disease, while an infection during a critical period of pregnancy can lead to severe congenital malformation, premature birth or stillbirth. The current knowledge about the virus, its diagnosis, the spread of the epidemic, the impact and the possibilities for preventing infections with SBV is described and discussed.     

Impact of maritime traffic and whale‐watching on apparent survival of bottlenose dolphins in the Strait of Gibraltar

1. The Strait of Gibraltar is an important habitat for cetaceans due to its high marine productivity. However, it is also the second most navigated channel in the world, subjecting cetaceans to a high level of vessel traffic, including an established whale‐watching fleet. Both maritime traffic and whale‐watching activities have been shown to impact the behaviour of cetaceans, but little is known about their impact on the demography and dynamics of cetacean populations.
2. The aim of this study was to evaluate the impacts of both ferry traffic and whale‐watching vessels on the apparent survival probability of a bottlenose dolphin (Tursiops truncatus) population that occurs in the Strait.
3. A Bayesian hierarchical mark–recapture modelling approach was applied to 8 years of photo‐identification data (2002–2009). Apparent annual survival probability was negatively correlated with ferry traffic, which explained >70% of temporal variation in survival, in contrast to whale‐watching, the effect of which was almost negligible. Despite these results, other natural and human‐related factors are likely to drive apparent survival in the study area.
4. Abundance increased between 2002 and 2005, and then decreased between 2006 and 2009, while local per‐capita recruitment decreased from 2004 until the end of the study period. These shifts correlated temporally with the construction of a large harbour on the Moroccan coast, which increased maritime traffic significantly, including a 40% increase in ferry traffic that regularly transited the Strait.
5. These results highlight the need to better understand the impact of maritime traffic on the demography of the dolphin population in the Strait, in order to implement evidence‐based conservation regulations in a region of high cetacean occurrence.

     

Wood preservation (carbon sequestration) or wood burning (fossil-fuel substitution), which is better for mitigating climate change?

Context

The effective ways of using wood production with a view to mitigating climate change are still disputed. Currently, there are two major opposing conceptions. One proposes to increase the carbon stock in forests, in wood products or in some kind of long-term wood storage, thus giving primacy to carbon sequestration. The other invokes the concept of biomass carbon neutrality to assert that the substitution of wood for fossil fuels avoids carbon emissions.

Aim and method

This paper contributes to this debate by comparing carbon footprints of heat generation when choosing wood or other fuels as alternatives.

Result

On condition that wood can be preserved with sufficient durability to meet the time frame of the necessary transition towards carbon-free energy resources (decadal to centennial time scales), one can demonstrate that the use of fossil fuels, with the exception of coal, is still preferable. The reasons are that the intrinsic carbon emission factor for wood has the highest value among all fuels in common use and that reference to the concept of wood carbon neutrality neglects the possibility of storing carbon positively in wood for a long time.

Conclusion

The conclusion is that to mitigate climate change it is better to store wood than use it as a fuel.     

Modification of Campylobacterjejuni Broiler Colonization by a Feed Additive Composed of Encapsulated Organic Acids and Essential Oils

The objective of this study was to evaluate the effect of a novel feed additive on chicken intestinal colonization and carcass contamination by Campylobacterjejuni. The feed additive was composed of microencapsulated organic acids and essential oils （OA/EO）. The feed additive tested was provided by Jefo Nutrition Inc., St-Hyacinthe, Quebec, Canada. Day-old birds were separated into two rooms and subdivided into two groups. Chicken were fed with OA/EO or not fed with OA/EO until they reached 35 d of age. At 14 d of age, chickens received an oral suspension of two well characterized C. jejuni strains, depending on the room they were housed in. The levels of C. jejuni were periodically monitored in the caecum and on the carcasses. C. jejuni colonization was further characterized by the use of high-resolution melt analysis of the C. jejuniflaA gene （HRM-flaA）. The effect of the feed additive was strain-dependent. In room two, the feed additive had no effect on the caecal counts. In room one, at 35 d of age, caecal C. jejuni counts were higher with OA/EO, as opposed to carcasses counts which were lower in the treated group. The HRM-flaA analysis showed that an amplification profile was predominant in birds fed with OA/EO at 35 d of age in room one, suggesting the selection of a C. jejuni strain. In conclusion, the OA/EO seemed to be effective to reduce C. jejuni levels but this effect appeared strain dependent.     

Adaptation of crop management to water-limited environments

Farmers must combine various crop management strategies to cope with water deficit resulting from soil, weather or limited irrigation: drought escape, avoidance or tolerance, crop rationing, irrigation (supplemental, deficit). These strategies can be translated into six objectives: (i) increasing soil stored water at plant sowing, (ii) increasing soil water extraction, (iii) reducing the contribution of soil evaporation to total water-use, (iv) optimizing the seasonal water use pattern between pre- and post-anthesis, (v) tolerate water stress and recover after stress alleviation, and (vi) irrigate at the most-sensitive growth phases. To reach these objectives, tactical decisions concern soil tillage, type of crop and cultivar, sowing date and density, N fertilization, irrigation timing, amount and frequency. Flexible crop management systems based on decision rules should be preferred to the recommendation of fixed packages of techniques. Timing, intensity, and predictability of drought (intermittent, terminal) are important features for choosing the cropping alternatives. Simulation models may help the farmer to select best-bet management options on the basis of historical long-term weather records. Simple soil and plant indicators associated with real-time decision support systems should be developed to revise the initial management plan by integrating in-season weather information.     

Effect of high temperature on the production of 2n pollen grains in diploid roses and obtaining tetraploids via unilateral polyploidization

To integrate the gene pool of a wild species (primarily diploid) into a cultivated pool (primarily tetraploid), a crossing between a dihaploid cultivated rose and a hybrid of Rosa wichurana allowed to obtain interspecific diploid hybrids that produced 2n pollen grains. A return to a tetraploid level sought by breeders can then be considered using sexual polyploidization, obtained by crossing a tetraploid cultivated rose with these hybrids. Application of a high‐temperature regime led to a small but significant increase in the percentage of 2n pollen grains in these hybrids of up to 4.6%. This result was obtained by applying high temperatures close to 32°C during the day to plants cultivated in a glasshouse during recurrent cycles of bloom. Crosses were made between an unreleased tetraploid hybrid tea rose, as a female, and the diploid hybrid that produces the most 2n pollen grains. Tetraploid (42.1%) and triploid (57.9%) offspring were obtained. The use of these 2n pollen grains of the first division restitution type should facilitate the introgression of complex traits of interest.     

DNA sequence polymorphisms and their application to bread wheat quality

Single-nucleotide polymorphisms (SNPs) constitute an abundant source of DNA polymorphisms, which have been successfully used to identify loci that are associated with a particular phenotype. Additionally, such markers could be efficiently used in combination with doubled haploid technology to improve the efficiency of breeding programmes. Information on such markers in plants is still scarce. For bread wheat, SNP data are restricted to a few genes. This can be explained by the hexaploidy of Triticum aestivum which makes SNP discovery difficult. We developed a novel method for SNP discovery in bread wheat. The strategy is based on the development of highly specific PCR-primers, which were used to sequence 27 lines. SNPs were discovered from sequence alignment data. Some SNPs were identified by mass spectrometry in a collection of 113 lines, which were both evaluated for agronomic traits and genotyped at 42 neutral microsatellite loci. Traits investigated include: protein content, the quantity of high-molecular-weight glutenins and that of the GluBx subunit. The 42 markers were used to infer population structure, which was included in linear models for association studies. The results of this preliminary study showed 89 SNPs in approximately 20 kbp, i.e., one SNP every 223 bp on average. Six SNPs were genotyped: three were located along the sequence of Glu-B1-1, while three non-synonymous SNPs were located along the sequence of the B homoeologous gene coding for SPA (Storage Protein Activator). The SNPs from Glu-B1-1 had a significant effect on the studied variables, whereas those of SPA had no effect. Such results might indicate that some haplotypes for Glu-B1-1 are linked to higher protein content, through an increased amount of high-molecular-weight glutenins, especially the GluBx subunit.     

The precautionary principle and biological invasion: the case of the House Sparrow on the Lesser Antilles

The capacity to react quickly to species introductions and to plan effective management countermeasures is always difficult, and methodology is lacking. This paper summarizes the different steps of a decision-making protocol applied to the recent establishment of an exotic species, the House Sparrow Passer domesticus, in the Lesser Antilles. This pest bird damages crops, fruits and stored food products and also competes with numerous hole-nesting birds in a large part of its range, and this requires more or less rapid management decision to limit its impact. In order to construct a base for decision-making in the Lesser Antilles case, we designed and conducted a brief biological study involving three spatial scales. At the regional scale, we defined the state of spread of House Sparrows throughout the West Indies. At the island scale, we estimated the number and distribution of breeding colonies. At the local scale, we estimated the numbers and behaviour of House Sparrows. To enable a quick decision to be made, we also suggested organizing a collective decision-making process applying a multi-actor guideline taking account of the different kinds of actors and levels of competence. We applied this method and we presented our results to a multi-actor workshop. The collective decision-making chose to apply the precautionary principle on one island (bird eradication) and to install both communications and monitoring plans. The procedure involving both multi-spatial scale assessment and a multi-actor workshop needs to be refined but its prospects are promising.     

Indole acetic acid and ACC deaminase-producing <Emphasis Type="BoldItalic">Rhizobium leguminosarum</Emphasis> bv. <Emphasis Type="BoldItalic">trifolii</Emphasis> SN10 promote rice growth,and in the process undergo colonization and chemotaxis

This study focuses on the chemotaxis, colonization and rice growth promoting ability of indole acetic acid (IAA) and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-producing rhizobacteria Rhizobium leguminosarum bv. trifolii SN10, previously isolated from root nodules of Trifolium alexandrium L. We show here that R. leguminosarum bv. trifolii SN10 promote the growth of four different rice varieties grown in India in terms of biomass, root branching and N content. In addition, using scanning electron microscopy and viable cell counts, we provide evidence that the bacteria successfully colonize the root surface of the rice variety which showed maximum growth promotion upon inoculation. Not only this, R. leguminosarum bv. trifolii SN10 exhibit a strong chemotaxis response towards the rice seed and root exudates despite the presence of a bacteriostatic phenolic compound, 7-hydroxycoumarin (umbelliferone). Further, R. leguminosarum bv. trifolii SN10 secretion of phytohormones such as IAA and ACC deaminase suggest the potential of the plant growth promoting rhizobacteria to be used as biofertilizer to enhance rice crop production in the subcontinent.     

A comparison of LiDAR-based DEMs and USGS-sourced DEMs in terrain analysis for knowledge-based digital soil mapping

While LiDAR-based digital elevation models (DEM) are more accurate and precise than the USGS-sourced DEM that are widely used in soil mapping in the US, their high cost and other problems prohibit an easy decision of adopting them in service-oriented soil mapping conducted by a government agency like USDA-NRCS. This study compares the performances of LiDAR-based DEM and the USGS-sourced DEM in calculating slope gradient as an input for knowledge-based digital soil mapping (KBDSM), aiming to provide scientific evidence and more importantly, propose a scientific approach to evaluating the two types of DEM for KBDSM. We conducted the comparison by evaluating how closely the DEM-based slope gradient values match the field-measured values. For a small watershed in northern Vermont, US, we prepared three DEM, including a 10-m DEM interpolated from the 7.5-minute USGS topographic map, a 1-m DEM based on LiDAR points, and a 5-m DEM resampled from the 1-m DEM. When calculating slope gradient, we applied two neighborhood sizes (10 m and 30 m), two neighborhood shapes (square and circular), and three slope gradient algorithms (Evans–Young, Horn, and modified Zevenbergen–Thorne) to the three DEM. We then compared the calculated slope gradient values with the values measured by soil scientists at 159 sample locations in the study area. Statistics show that across all the tested settings, the LiDAR-based DEM perform significantly better than the USGS-sourced DEM. We conclude that LiDAR-based DEM may considerably improve the quality of inputs for KBDSM. We also find that the results from the 1-m LiDAR-based DEM and the resampled 5-m DEM do not show considerable and consistent differences.