Morphological changes during the transition from freshwater to sea water in an amphidromous goby,Sicyopterus lagocephalus (Pallas 1770) (Teleostei)

The widely distributed amphidromous goby Sicyopterus lagocephalus needs drastic change of habitat to fulfil its life cycle: adults live and spawn in rivers, where eggs hatch into prolarvae that have to reach the sea to acquire characteristics of planktonic larvae. Postlarvae return to rivers where they recruit and grow to the adult reproductive stage. Here, we describe the prolarval stages, namely from hatching to first contact with sea water, as well as the first marine larval stages. The observations were made under experimental conditions. We described 3 prolarval substages in freshwater (L1a–L1c). Prolarvae present a slight but visible ontogenetic development in freshwater, during which the yolk sac begins to reduce, the pigmentation increases on the body and in the eyes, and the lenses appear, although the eyes are not functional. Prolarvae need to reach the sea in a maximum of 96 h to pursue their development. Their transfer in sea water at a salinity of 36.5 induces important morphological modifications (i.e. yolk sac full absorption, appearance of pectoral fins, migration of the eyes in anterolateral position of the head, opening of mouth and anus), enabling the organisms to adapt to their new environment. This marine stage is divided into two substages: L2a corresponding to the organisation of the morphological structures adapted to the marine environment and L2b during which these morphological structures become functional. Whether it is in freshwater or sea water, the duration of the substages depends on the water temperature, but is similar for all individuals for a given temperature.     

A decision support system to simulate and compare silvicultural scenarios for pure even-aged larch stands

In sustained forest management, it is particularly useful to test the adequacy of various silvicultural scenarios, but decision-making is also becoming increasingly complex because forest managers have to simultaneously meet several different objectives in response to society demands. In order to help forest managers make appropriate choices in silvicultural systems, we propose a SDSS (Silvicultural Decision Support System) that applies to pure and even-aged larch stands (Larix sp.) in lowland areas where site conditions are similar to those encountered in Southern Belgium. Its main purpose is (i) to predict the influence of silvicultural treatments on stand evolution and (ii) assist forest managers in comparing different scenarios with respect to predefined goals. This SDSS consists in three modules designed to elaborate silvicultural scenarios involving (i) stand growth prediction and thinning simulation; (ii) assessment of indicators selected to define the scenarios referring to wood production, financial, technico-economic and ecological components, tree stability and wood quality; and (iii) comparison of the scenarios on the basis of the set of previously assessed indicators (multi-criteria analysis). User-friendly “MGC_Larch” software helps managers to formulate, evaluate and compare different silvicultural scenarios for larch.     

Visualization of mixing flow phenomena in champagne glasses under various glass-shape and engravement conditions

For the very first time, a classical flow visualization technique was used to capture the fluid motion in traditional flutes and coupes poured with champagne. It was found that glasses engraved around their axis of symmetry produce a rising gas column along the vertical glass axis that induces, in turn, steady state recirculating flow regions. In the case of the classical engraved champagne flute, the whole domain of the liquid phase is homogeneously mixed, whereas in the case of the engraved champagne coupe, the recirculating flow region does not occupy the whole volume in the glass. In the engraved coupe, a dead-zone of very low motion was identified, which inhibits the formation of the collar at the glass edge. Our results finally strongly suggest that the glass-shape and engravement conditions should likely have a strong impact on champagne tasting by modifying the kinetics of release of carbon dioxide molecules and aromatic volatile organic compounds from the liquid medium.     

Effectiveness of an oral hygiene chew to reduce dental deposits in small breed dogs

Over a period of 4-months, feeding small breed dogs with a regular dry diet supplemented with a daily oral hygiene chew resulted in a significant reduction in plaque deposition (17.3%) and calculus accumulation (45.8%) compared with dogs fed the dry diet alone. Daily feeding of a specifically designed oral hygiene chew that reduces plaque and calculus accumulation may play a role in the severity of canine periodontal disease.     

Haemonchus contortus (Nematoda: Trichostrongylidae) infection in lambs elicits an unequivocal Th2 immune response

Selection of resistant animals and host immunization have been proposed as alternative methods for the control of gastrointestinal nematode parasites. However, a better knowledge of the mechanisms involved in protective immunity against these parasites is required for the development of optimal strategies. In this study, 3 month old INRA 401 lambs (n = 81) were allocated into three groups: uninfected control, challenged either once (primary-infected animals) or twice (previously-infected animals) with 10,000 Haemonchus contortus L3. Uninfected control and challenged animals were sequentially sacrificed at 3, 7, 15 and 28 days post challenge. In both challenged groups, a clear Th2-oriented immune response was observed in the abomasal lymph node and mucosa. IL-4 and IL-13 mRNA over-expression, recruitment of eosinophils, mast cells and globule leukocytes and production of specific systemic IgG and mucosal IgA were observed earlier in previously-infected animals than in primary-infected ones. At 28 days post infection, no differences between intensities of these responses were observed between the challenged groups. Worm establishment rates were similar in previously-infected and primary-infected lambs. However, reductions of worm development, female fecundity and fecal egg output were observed in previously-infected sheep. We conclude that H. contortus infection in young INRA 401 lambs induced an unequivocal Th2 immune response resulting in the regulation of worm egg production without affecting their establishment.     

Quantitative analysis of beta-sitosterol oxides induced in vegetable oils by natural sunlight, artificially generated light, and irradiation

UV radiation is able to induce lipid peroxidation. Photooxidation-induced beta-sitosterol oxides were monitored in four vegetable oils exposed to sunlight for 10, 20, and 30 days during May 2005 (northeastern France), exposed to artificial light generated by a high-pressure Hg lamp for 21, 42, and 63 h at room temperature, and exposed to a 10 MeV electron beam at 0.93, 2.69, and 9.30 kGy at 8 degrees C. Quantification was performed by capillary gas chromatography-mass spectrometry according to the total ion current mode and using a reconstructed ion trace chromatogram with specific ion fragments. Sunlight induced the formation of higher amounts of oxides than UV light, while no significant oxidizing effect was observed with electron beam irradiation. However, data suggested that the amount of the main oxides formed was strongly dependent on the dose rate (length of exposure). Accordingly, shorter but more intense treatments had lower oxidizing effects.