辣椒全基因组WRKY转录因子的分析

基于已公布的辣椒全基因组数据,利用生物信息学方法对辣椒WRKY转录因子家族进行全面鉴定和系统命名,并在此基础上对基因分类、染色体定位、系统进化关系和结构域序列保守性进行了研究。结果表明:辣椒CaWRKY家族包含71个基因,根据WRKY结构域的数量及锌指结构的特征可将其分为GroupⅠ、GroupⅡ和GroupⅢ等3大类,GroupⅡ又可分为Ⅱ(a)、Ⅱ(b)、Ⅱ(c)、Ⅱ(d)和Ⅱ(e)等5个亚类。辣椒12条染色体上均有WRKY转录因子分布,其中第1号染色体上分布最多,共有10个,第4号染色体上分布最少,仅有2个。辣椒每类/亚类WRKY几乎含有相同的保守基序。辣椒WRKY编码的蛋白在132~869个氨基酸范围内,平均氨基酸数量为373个。     

用于黄瓜白粉病抗性鉴定的InDel标记

根据黄瓜白粉病抗性主效基因/QTL的插入/缺失突变,将其设计成共显性的In Del分子标记。通过群体连锁分析,发现In Del-MLO1标记与黄瓜白粉病抗性主效基因共分离。应用该标记对24份来自世界各地且白粉病抗性已知的黄瓜材料进行抗性验证,21份材料的抗性表型与标记的带型一致,说明在黄瓜进化和驯化过程中,造成抗病表型的该插入/缺失突变发生普遍。因此,该In Del标记适合于大多数黄瓜材料的白粉病抗性鉴定和分子标记辅助育种,从而加快黄瓜白粉病抗病育种的进程。     

花毛茛新品种“橙色年代”的选育

"橙色年代"是以"乐园"花毛茛为亲本经混交、杂交选育而成的新品种。该品种平均株高23.6cm,平均花径14.6cm,极适合盆栽;平均每株花数5.5朵,平均花期54.5d。在-5℃环境下无冻害,综合抗性好,繁殖能力强。适合江苏省全境及长三角地区保护地栽培。     

营养液条件下油酸对黄瓜幼苗生长的影响

水培条件下，研究不同油酸水平对黄瓜幼苗生长的直接影响。与对照相比，生物量和生理指标等表明，油酸10μL/L水平对黄瓜生长几乎没有负面影响，而油酸水平为100，500，1000μL/L则表现出一定的抑制效应。其中，1000μL/L水平中黄瓜的生长明显受到抑制。试验还表明，油酸对黄瓜幼苗没有表现出直接的生长刺激效应。     

天祝白牦牛KAP1.1基因亚型B2A克隆及鉴定

通过对天祝白牦牛高硫角蛋白基因启动子B2A克隆得到其序列,将序列提交到NCBI,登录号:KJ910005,并对其进行生物信息学分析,通过亚细胞定位,磷酸化分析,二级、三级结构和保守结构域预测结果比较,MEGA 5.10软件进行NJ法进化树构建和Meg Align进行蛋白质相似分析来分析B2A基因与KAP1.1(Keratin associated protein1.1)基因的差异性。B2A基因的CDS区全长为471 bp,共编码156个氨基酸,二级结构含有延伸链、β转角和无规卷曲3种。B2A蛋白和KAP1.1蛋白的亚细胞定位,磷酸化分析,保守结构域,二级结构、三级结构和同源性差异较大。但是从功能预测结果显示2个蛋白功能完全一致,并且使用Clustal X进行氨基酸序列比对分析显示B2A蛋白从第33位开始相对于KA1.1蛋白有10个氨基酸的缺失以外,其余序列之间的保守性相当高。因此,可以证明B2A基因是KAP1.1基因的基因亚型。     

高产优质多抗玉米杂交种黔单20号制种技术

根据黔单20号亲本在贵州山区生态条件下的特征特性以及制种过程中的产量表现，总结出该品种的制种经验，为我省及周边地区该品种制种提供参考。     

纳雍珙桐自然保护区珍稀濒危植物资源初步研究

通过野外调查及标本采集,对纳雍珙桐自然保护区珍稀濒危植物的分布特征、生态习性、利用价值、资源现状进行了初步研究并对其濒危程度和致危原因进行初步探讨。结果表明,该保护区共有珍稀濒危植物16科35属43种(包括变种)。其中国家Ⅰ级保护植物3属3种,国家Ⅱ级保护植物8属8种;《濒危野生动植物种国际贸易公约(附录Ⅱ)》(CITES)1科16属24种;贵州省重点保护树种8属8种。     

Research on PDM Technology Oriented Dynamical Data

对冬枣采后主要病害种类、症状及防治方法进行阐述,并介绍了生物防治技术在果蔬贮运病害防治中的应用,展望了该技术在冬枣采后病害防治中的应用前景.     

3-D Simulation on the Transient Heat Transfer Characteristics of Municipal Solid Waste Lump in Clean Incinerator

To be effectively heated in incinerator for MSW is very important for stabilization and completeness combustion, the smaller produce of the PCDD/Fs. At the same time, the transient heat transfer characteristics of the MSW lump decides the stay time of the MSW in incinerator and effects on the operation feature of the combustion equipments. All of these would lead to design the reasonable and clean combustion project, the optimum incinerator and recover energy system. The component, shape, dimension, property, the hardly and inner structure feature of the MSW lump are taken in account, and the effective conduct heat coefficient has been adopted. And then, the physical model is setup with different shape(ball, cylinder, squareness), and the 3-D mathematical model and conduct heat efficient with porous feature for transient heat transfer of MSW lump are presented, at the same time, the simulation tests are gotten in various boundary and the interesting conclusions are gotten: The center stable temperature of the MSW lump is mostly decided by the inner gas temperature of the incinerator and the heated area of the lump. The transient heat transfer characteristics is variously effected by the shape of the MSW lump in incinerator. The transient process becomes longer and the center stable temperature decreases, the volatile can't be easily separated out and the combustion efficiency decreases because of the increasing of the lump feature dimension.     

Mechanical Performance and Thermology Performance of 20CrMo Steel at High Temperature

Employing the Gleeble-1500D thermal analogue machine and the STA449 synthesis thermal analyzer,the study on hot ductility and parts of thermology performance of 20CrMo steel has been carried out , many consulted foundation data has been obtained, the optimum ductility temperature arrange has been found, contraction of cross sectional area is only 30% between 600 degree and 775 degree, but contraction of cross sectional area is more than 80% between 800 degree and 1 250 degree, contraction of cross sectional area drops to under 60% rapidly between 1 350 degree and melting point,a curve is drawn showing the relationshi Pbetween heat capacity and the variation of temperature for the first time, the counting formulas of the heat capacity are regressed by disjunction. A curve is drawn showing the relationshi Pbetween the coefficient of thermal expansion and the variation of temperature, and their application in continuous casting is discussed herein.