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991.
992.
F. Parrot 《Potato Research》1975,18(3):446-450
Résumé L’addition de sels minéraux dans le milieu de culture retarde la tubérisation des fragments de tige de pomme de terre. L’AG
renforce cet effet tandis que le CCC le contrarie. Les modifications de croissance apportées par ces différents facteurs sont
également considérées. Un essai d’interprétation est proposé. 相似文献
993.
Plasma and joint fluids from turkeys experimentally inoculated with Chlamydia psittaci strain TT3 were evaluated by immunoblotting to identify antibodies elicited by chlamydial antigens during the course of infection. Protein antigens from elementary bodies of TT3 were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred electrophoretically to nitrocellulose before being probed with plasma or synovial fluid from TT3-inoculated birds. The major outer-membrane protein (MOMP), the 60,000-molecular-weight proteins, and a 97,400-molecular-weight protein were the predominant antigens recognized by IgG in the plasma and joint fluids. Plasma IgG specific for the 97,400 protein band was first detectable at day 10 postinoculation (PI). Antibodies to the 60,000-molecular-weight protein and MOMP were first detected at days 14-17 PI and at days 7-10 PI, respectively, in some birds, and as late as days 36-42 PI and days 42-70 PI in others. The antibodies were still present at day 142 PI. Immunoblotting techniques indicated that the antigens to which these antibodies were reacting were protein. These observations may have implications for the development of serodiagnostic assays as well as the identification of potential proteins for subunit immunogens in birds. 相似文献
994.
van Beurden SJ Leroy B Wattiez R Haenen OL Boeren S Vervoort JJ Peeters BP Rottier PJ Engelsma MY Vanderplasschen AF 《Veterinary research》2011,42(1):105
ABSTRACT: Many of the known fish herpesviruses have important aquaculture species as their natural host, and may cause serious disease and mortality. Anguillid herpesvirus 1 (AngHV-1) causes a hemorrhagic disease in European eel, Anguilla anguilla. Despite their importance, fundamental molecular knowledge on fish herpesviruses is still limited. In this study we describe the identification and localization of the structural proteins of AngHV-1. Purified virions were fractionated into a capsid-tegument and an envelope fraction, and premature capsids were isolated from infected cells. Proteins were extracted by different methods and identified by mass spectrometry. A total of 40 structural proteins were identified, of which 7 could be assigned to the capsid, 11 to the envelope, and 22 to the tegument. The identification and localization of these proteins allowed functional predictions. Our findings include the identification of the putative capsid triplex protein 1, the predominant tegument protein, and the major antigenic envelope proteins. Eighteen of the 40 AngHV-1 structural proteins had sequence homologues in related Cyprinid herpesvirus 3 (CyHV-3). Conservation of fish herpesvirus structural genes seemed to be high for the capsid proteins, limited for the tegument proteins, and low for the envelope proteins. The identification and localization of the structural proteins of AngHV-1 in this study adds to the fundamental knowledge of members of the Alloherpesviridae family, especially of the Cyprinivirus genus. 相似文献
995.
996.
A case of selenium poisoning was suspected following the administration of 325 micromol selenium a day as sodium selenate (25 mg Se) for five consecutive days. The main signs were confined to changes in the integument which were loss of hair from the mane and tail, disintegration of the skin of the anus, lips, prepuce and scrotum, and separation of the hooves from the coronary corium. Periodic analyses of blood, hair and hoof parings from the sole correlated strongly with blood and identified horn and hair as important additional routes of excretion. 相似文献
997.
998.
D C Baker L F James W J Hartley K E Panter H F Maynard J Pfister 《American journal of veterinary research》1989,50(8):1396-1399
Three groups of 5 pigs each were fed a high selenium (Se) diet by mixing either Astragalus praelongus (31.6 ppm Se in feed), A bisulcatus (31.7 ppm Se in feed), or sodium selenate (26.6 ppm Se in feed) with commercial hog feed. Ten control pigs were fed only commercial hog chow containing trace selenium (0.44 ppm Se). Pigs were fed for 9 weeks and necropsied when they had ataxia or paralysis. Blood was collected for hematologic and serum biochemical determinations, and samples of various tissues were collected and fixed in neutral-buffered 10% formalin for histologic evaluation or frozen for determination of selenium concentration. All forms of selenium induced clinical signs of weight and hair loss, with cracked hooves and inflamed coronary bands developing in all Na2SeO4-fed pigs and 1 A praelongus-fed pig, but not in A bisulcatus-fed pigs. Serum calcium, phosphorus, and albumin concentrations were unchanged or significantly decreased from prefeeding values in groups fed selenium. Serum aspartate transaminase (AST) activities in Astragalus species-fed groups, and amylase activities and PCV in all groups of pigs fed selenium, were increased. Serum alkaline phosphatase and creatine kinase activities were significantly increased in the A praelongus-fed pigs and significantly decreased in Na2SeO4-fed pigs. Terminal tissue and body fluid selenium concentrations were determined in all groups of pigs fed selenium and compared with values in control pigs. Urine and bile concentrations were increased by the greatest factor (40 to 100x), with tissue concentrations of selenium increased by a lesser factor (6 to 17x).(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
999.
1000.
Mestorino N Formentini EA Lucas MF Fernandez C Modamio P Hernández EM Errecalde JO 《Veterinary research communications》2008,32(1):21-33
A comparative pharmacokinetic study was conducted to determine the order and the rate of absorption of triclabendazole (TCBZ)
in cattle and sheep. A commercial suspension of TCBZ (Biofasiolex, Biogénesis S.A., Argentina) was administered at a dose
rate of 10 mg/kg by the oral route to six Holstein female calves and six Corriedale female sheep. The plasma concentration
profiles of the metabolites triclabendazole sulfoxide (TCBZ-SO) and triclabendazole sulfone (TCBZ-SO2) were analysed by means of the non-compartmental method. The order of the absorption process of the active metabolite, TCBZ-SO,
was determined by construction of curves of cumulative absorbed fraction of the drug by means of the Wagner-Nelson method.
The appearance of TCBZ-SO in plasma of cattle and sheep resembles the entry of a constant quantity of drug into the organism
per unit time. This is explained by the reservoir effect of the rumen, which acts as a biological slow-release system for
TCBZ-SO and its precursor TCBZ to the posterior digestive tract where they are absorbed. The plasma concentration profiles
of TCBZ-SO in both species were well described by a one-compartment open model with zero-order process of absorption and first-order
process of elimination. The values of AUC0-∞ and C
max of TCBZ-SO did not differ between species, while other kinetic parameters except for λ
z
had higher values in calves than in sheep. In the case of TCBZ-SO2, t
max was the only parameter that did not differ between species, while other kinetic parameters except for λ
z
had higher values in calves than in sheep. 相似文献