整合素αvβ3在人工受精奶牛受孕前后表达规律研究

本试验通过对10头荷斯坦奶牛在受孕前后的整合素αvβ3表达变化规律与奶牛早期妊娠关系的研究,筛选早期妊娠检测标志性因子,从而提高奶牛的繁殖效率。试验表明,选取的10头荷斯坦奶牛中有7头受孕3头未受孕,检测发现,10、13、16、19d,αv基因和β3基因转录及整合素αvβ3表达水平在受孕奶牛血液中的表达量均高于未孕奶牛,特别是输精后第16d受孕奶牛的表达量显著高于未孕奶牛。因此,αvβ3检测可将早孕检测的时间窗口提前到人工输精后第16d。     

圈养象的足部护理实用要点

足部护理是圈养象饲养管理中不可或缺的部分,对保障圈养象健康、提升个体福利方面发挥着非常重要的作用。本文参考相关文献资料,结合太原动物园现已开展的圈养象足部护理实践,总结了包括日常检查及清洁、趾甲护理、脚掌护理、角质护理及脓肿处理5项最主要的足部护理工作,探讨了各项工作的护理要点、护理频率及工具选用等实践操作流程,旨在为动物园行业开展圈养象足部护理提供参考,提高圈养象福利。     

Up-regulation of Dicer1 gene expression by prostate-specific transcriptional factor NKX3.1

AIM: To investigate the effect of NKX3.1 on the Dicer1 gene expression.METHODS: The NKX3.1 eukaryotic expression plasmid was transfected into PC3 cells. The stable clones were isolated using cloning cylinders and grew continuously under G418 selection. The gene expression profile in PC3 (+) cells induced by NKX3.1 was analyzed by cDNA microarray. The effect of NKX3.1 on the Dicer1 expression was further investigated by RT-PCR and Western blotting in PC3 and PC3 (+) cells according to the results of gene chip. To determine if the increase in Dicer1 promotes the mature of microRNA, the pMIR-report luciferase expression plasmid of miRNA let-7a1 target sequence (pMIR-report-let7a1T) was constructed and transfected into PC3 and PC3 (+) cells. The effect of the miRNA let-7a-1 on its target sequence was determined by luciferase reporter assay.RESULTS: The result of gene chip showed that the expression level of Dicer1 gene was higher in PC3 (+) cells than that in PC3 cells. The results of RT-PCR and Western blotting indicated that the expression of Dicer1 gene was much higher in PC3 (+) cells than that in PC3 cells. The relative luciferase activity was much lower in PC3 (+) cells than that in PC3 cells when the cells were transfected with the pMIR-report-let7a1T vector.CONCLUSION: Up-regulation of Dicer1 expression induced by NKX3.1 promotes the mature and functions of microRNAs in prostate cancer PC3 cells.     

葛根渣栽培香菇培养料配方优化试验

为了实现葛根渣的资源化利用,降低香菇生产成本,试验用葛根渣按不同比例代替常规培养料中木屑栽培香菇。结果表明,供试配方香菇均能生长,但香菇长势和产量有明显的差异,其中以配方3(葛根渣40%,木屑40%,麸皮17%,蔗糖1%,石膏1%,碳酸钙1%)培养料栽培的香菇子实体产量及品质均优于其他配方。     

基于芡实叶脉分叉结构的穹顶温室设计与试验

基于芡实叶脉的分叉结构及三角形的稳固特性设计了一分二-原、不分叉、一分二-全三角形和一分三4种穹顶温室。利用ANSYS有限元分析软件对4种温室进行了静力及线性屈曲分析,静力分析结果表明,4种温室在组合2(风载组合)作用下差别显著,即随着分叉级数增多,位移与强度分别降低了98. 92%和86. 46%,一分二-全三角形和一分三满足强度要求,且两者力学特性相近,耗材量相比不分叉结构分别增加了11. 88%和30. 18%;屈曲分析结果表明,竖直和水平荷载作用下,屈曲荷载分别是许用荷载的63. 52～26 737. 22倍与26. 86～11 300. 97倍(以单元长度为限),4种温室稳定性皆满足要求,且对竖直荷载响应显著,即分叉结构使屈曲荷载平均增加了334. 02%;综合得出一分二-全三角形为最优结构。基于第二相似理论,以1∶25制作的缩尺模型在12组应变试验中,与仿真结果无显著差异的组数占75%,25%略有偏差,试验相对误差为0. 18%～0. 69%,仿真过程准确可靠;模型横梁对组合1(雪载组合)响应明显,即各组应变均值在组合1作用是组合2作用下的5. 6倍,竖梁对组合2响应明显,即各组应变均值在组合2作用是组合1作用下的6. 2倍。     

基于外力估计的并联机器人柔顺控制策略研究

针对并联机器人在作业过程中的位置精确控制及柔顺控制问题,提出了基于外力估计的并联机器人柔顺控制策略,实现并联机器人在作业过程中位置和力的高性能动态交互。基于外力估计的柔顺控制实现过程中,考虑到接触力传感器成本较高,提出一种无传感器外力估计的方法。首先建立并联机器人以及伺服运动系统动力学模型,利用所建立的动力学模型和电机的电流反馈值来估算外力作用时机器人关节力的变化。其次根据估算的并联机器人关节力,设计基于位置的阻抗控制,使并联机器人末端执行器与环境柔性接触,确保并联机器人的作业精准度与柔顺度。最后选取合适的阻抗控制参数,对所提出的柔顺控制策略进行仿真分析并且在搭建的实验平台上进行了实验验证,实验结果表明所提出的方法可以实现并联机器人的精确柔顺作业。     

两个棉花几丁质酶基因的克隆与表达分析

 从棉花黄萎病缩减杂交库中得到了分属Ⅲ型和Ⅳ型几丁质酶的两个片段,它们都具有完整的3’末端。通过RACE与RT-PCR结合获得了这2个基因完整的读码框序列。其中Ⅳ型几丁质酶的开放读码框为678 bp,编码长为226个氨基酸的蛋白,命名为Ghachi4;Ⅲ型几丁质酶的全长为1390 bp,编码长为298个氨基酸的蛋白,命名为Ghachi3。它们与拟南芥同源基因的相似性分别达到65%和71%。利用网上分析软件发现这2个基因都有信号肽序列,并预测编码的蛋白位于胞质体外。半定量RT PCR发现Ghachi3在花、蕾和韧皮部中的表达量较高,而Ghachi4在韧皮部表达量最高。Ghachi4仅在抗病品种受黄萎病和枯萎病的诱导后表达上调,而Ghachi3还在感病品种中受黄萎病的诱导表达。同时,在常抗棉中2个基因的表达都受到ABA的强烈诱导。推测这2个基因可能参与生物胁迫或非生物胁迫的防御反应。     

Effect of S-nitrosylation induced by IL-1β and IFN-γ on DNA binding activity of cAMP response element binding protein in fibroblast-like synoviocytes

AIM: To investigate the effect of S-nitrosylation induced by recombinant interleukin-1β (rIL-1β) and recombinant interferon-γ (rIFN- γ) on DNA binding activity of cAMP response element binding protein (CREB) in fibroblast-like synoviocytes (FLSs). METHODS: (1)FLSs were incubated with rIL-1β and rIFN-γ in the presence or absence of inducible nitric oxide synthase inhibitor aminoguanidine (AG) for 12 h. The supernatant of the cell culture was collected to determine the contents of nitric oxide (NO). The total proteins prepared from each group ［only the total proteins prepared from rIL-1β+rIFN-γ group was reacted with dithiothreitol (DTT) for 15 min in vitro］ were subjected to the biotin switch assay, and the level of S-nitrosylation was determined by Western blotting. (2)FLSs were incubated with rIL-1β, rIL-1β+ rIFN-γ and AG respectively for 12 h. The nuclear extracts from each group were prepared. The nuclear extracts from each group were subjected to electrophoresis mobility shift assay to analyze the DNA binding activity of CREB (only the nuclear extracts from rIL-1β +rIFN-γ group was reacted with DTT for 15 min in vitro before assaying). RESULTS: rIL-1β plus rIFN-γ increased the production of NO and the level of S-nitrosylation, which was inhibited by AG. Administration of DTT in the total proteins reversed the induction of S-nitrosylation by rIL-1β and rIFN-γ. Co-incubation with rIL-1β and rIFN-γ inhibited the CREB activity induced by rIL-1β alone, which was reversed by AG. Administration of DTT in the nuclear extracts reversed the effect of co-incubation of the cytokines. CONCLUSION: Co-incubation with rIL-1β and rIFN-γ may increase the level of S-nitrosylation through inducing the production of endogenous NO, leading to reversible thiols modification of CREB and inhibit the DNA binding activity of CREB in FLSs.     

提高工程质量方法初探

针对目前建筑工程质量存在的问题 ,探讨了一些结合实际的提高工程质量的方法。