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71.
Acetylcholinesterase (AChE) was purified by affinity chromatography from two populations of the oriental migratory locust, Locusta migratoria manilensis (Meyen), collected from Huanghua and Pingshan Counties, Hebei Province of China. The purification factors and yields were 1661-fold and 19.3%, respectively, for the Huanghua population, and 3897-fold and 39.6% for the Pingshan population. Both the purification factor and yield were significantly lower in the Huanghua population than in the Pingshan population. AChE activity was almost completely inhibited by 10−6 M eserine and BW284C51, but ?5.8% of AChE activity was inhibited by ethopropazine at the same concentration, suggesting that purified AChE from either population was a typical insect AChE. However, AChE purified from the Huanghua population was 62-, 2.0-, and 1.6-fold less sensitive to inhibition by the three organophosphate compounds, chlorpyrifos oxon, demeton-S-methyl, and paraoxon, respectively, than that from the Pingshan population. Significantly lower purification factor and low yield associated with reduced sensitivity of AChE to inhibition by the organophosphates indicated that AChE purified from the Huanghua population was biochemically and pharmacologically different from that of the Pingshan population. Reduced sensitivity of AChE appeared to contribute to organophosphate resistance in the locust from Huanghua County, where insecticides have commonly been used to manage outbreaks of the locust. 相似文献
72.
肉鸡业是我国畜牧业的重要组成部分,肉鸡产业化经营推动了地方农村经济的发展,同时也大大降低了肉鸡业的投资和生产成本,我国肉鸡业正在向高度集约化、工厂化的方向发展。像其他工业生产一样,这种高效率生产肉产品的企业也存在废物处理、产品的安全性及生产过程中对大气、水体和土壤的污染问题,这些问题如果处理不当就会造成公害。本文就目前肉鸡业存在的公害, 相似文献
73.
绵羊肺腺瘤病毒NM株前病毒gag基因的克隆与序列分析 总被引:4,自引:0,他引:4
参照GenBank中已发表的绵羊肺腺瘤病毒(JSRV)的全基因序列,设计合成3对引物,对JSRVNM株的gag基因分3段进行PCR扩增,经琼脂糖凝胶电泳分析,分别呈3条531、888和949 bp的特异条带,将其分别克隆人pMD-18T载体中,进行序列测定并拼接序列,得到完整的gag基因序列。分析结果表明,与南非代表株(基因序列号NC-001494)的gag基因序列比较,核苷酸同源性为89.0%,推导出的氨基酸同源性为90%。与美国代表株(基因序列号AF105220)的gag基因序列比较,核苷酸同源性为86.3%,氨基酸同源性为87%。 相似文献
74.
Qiu-yan Chang Fu-cheng Guo Xue-rui Li Jian-hua Zhou Xuepeng Cai Qiuwei Pan Xiao-xia Ma 《Veterinary research communications》2018,42(4):309-313
Peste des petits ruminants virus (PPRV) causes highly contagious diseases in domestic and particular wild small ruminants, leading to substantial economic loss. The development of effective and cheap antiviral medications shall help to circumvent this emerging burden. In this study, we found that ribavirin, a competitive inosine-5’-monophosphate dehydrogenase (IMPDH) inhibitor, significantly inhibits the replication of PPRV. As IMPDH is a key enzyme in purine nucleotide synthesis, supplementation of exogenous guanosine attenuate the anti-PPRV effect of ribavirin. Interestingly, an uncompetitive IMPDH inhibitor, mycophenolic acid (MPA), exerted more potent antiviral effect again PPRV. Similarly, this effect was largely restored upon supplementation of guanosine. Thus, we have demonstrated that the IMPDH inhibitors ribavirin and MPA combat PPRV infection through purine nucleotide depletion. Because both regimens have been widely used in the clinic for treating viral infection or organ rejection in transplantation patients for decades, respectively, repurposing these existing safe and cheap medications may provide a new avenue for combating PPRV infection. 相似文献
75.
Based serum metabolomics analysis reveals simultaneous interconnecting changes during chicken embryonic development
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M. L. Peng S. N. Li Q. Q. He J. L. Zhao L. L. Li H. T. Ma 《Journal of animal physiology and animal nutrition》2018,102(5):1210-1219
Metabolic disorder is a major health problem and is associated with a number of metabolic diseases. Due to native hyperglycaemia and resistance to exogenous insulin, chickens as a model had used in the studies of adipose tissue biology, metabolism and obesity. But no detailed information is available about the comprehensive changes of serum metabolites at different stages of chicken embryonic development. This study employed LC/MS‐QTOF to determine the changes of major functional metabolites at incubation day 14 (E14d), 19 (E19d) and hatching day 1 (H1d), and the associated pathways of differential metabolites during chicken embryonic development were analysed using Metabolite Set Enrichment Analysis method. Results showed that 39 metabolites were significantly changed from E14d to E19d and 68 metabolites were significantly altered from E19d to H1d in chicken embryos. Protein synthesis was promoted by increasing the concentrations of L‐glutamine and threonine, and gonadal development was promoted through increasing oestrone content from E14d to E19d in chicken embryos, which indicated that serum glutamine, threonine and oestrone contents may be considered as the candidate indicators for assessment of early embryonic development. 2‐oxoglutaric acid mainly contributed to enhancing the citric cycle, and it plays an important role in improving the growth of chicken embryos at the late development; the decreasing of L‐glutamine, L‐isoleucine and L‐leucine contents from E19d to H1d in chicken embryonic development implied their possible functions as the feed additive during early posthatch period of broiler chickens to satisfy the growth. These results provided insights into understand the roles of serum metabolites at different developmental stages of chicken embryos, it also provides available information for chicken as a model to study metabolic disease or human obesity. 相似文献
76.
77.
基于冗余分析的高寒草原土壤与草地退化关系 总被引:2,自引:0,他引:2
在高寒草原退化草地设置研究样地,观测植物群落特征、采集土壤样品、分析土壤物理化学性质,依据数量生态学基本原理,探讨高寒草原退化草地与土壤因子间关系。结果表明:不同退化程度样地沿冗余分析排序图第一排序轴分布,第一排序轴反映草地退化程度的变化;草地植物群落中与第一排序轴负相关且按相关程度大小排序的指标为盖度>地上生物量>地下生物量;第一、第二排序轴能够解释97.1%的土壤因子与草地退化关系;土壤沙砾、p H与第一排序轴正相关,土壤有机碳、土壤含水量、容重、全氮、有效氮与第一排序轴负相关;第一排序轴及所有排序轴所反映的土壤因子均与草地退化之间呈极显著相关关系(P<0.01);不同土壤因子与草地退化之间相关程度不同,土壤有机碳(r=-0.890)、土壤含水量(r=-0.864)、容重(r=-0.847)、全氮(r=-0.836)、有效氮(r=-0.821)等与高寒草原退化相关程度更高且相关关系极显著(P<0.01)。利用退化草地群落特征和土壤因子数据矩阵进行冗余分析能够较好地综合反映土壤因子与草地退化之间的关系及相关程度,土壤有机碳、土壤含水量、容重、全氮、有效氮是反映高寒草原退化的重要土壤指标。 相似文献
78.
玛曲高寒草甸放牧强度的遥感监测 总被引:1,自引:0,他引:1
为给玛曲县高寒草甸草-畜平衡和草地资源的可持续利用提供科学依据,本研究利用遥感技术,结合2016年地面实测数据和同时期的MODIS影像资料,分别建立了研究区草地地上生物量和植被指数(NDVI)、相对放牧强度间的回归模型以及不同相对放牧强度和NDVI值之间的幂回归模型。结果表明,6-9月调查点NDVI和地上生物量间存在较好的相关性,即随着NDVI值的增加生物量呈上升趋势,且9月份的拟合(R2=0.510 2)达到了极显著水平;实测地上生物量和相对放牧强度之间有很好的拟合关系(R2=0.965 1),随着放牧强度的增加地上生物量呈下降趋势;相对放牧强度和植被指数间相关性较好(R2=0.631),即随着NDVI值的变小,相对放牧强度逐渐增强,当相对放牧强度增强到一定程度时,NDVI对其响应不灵敏。 相似文献
79.
为了解新疆乌鲁木齐市犬布鲁氏菌病流行特征,分析犬感染风险因素,初步掌握养犬人员对布鲁氏菌病的认知程度和行为特点,从而提出合理的预防对策和控制措施,采用流行病学横断面研究方法,通过估计流行率计算抽样数量,然后随机抽样,采用IELISA方法检测光滑型和粗糙型犬布鲁氏菌,同时结合调查问卷,获得乌鲁木齐市犬布鲁氏菌病流行率、感染风险因素和养犬人员对布鲁氏菌病认知情况,并对调查结果采用SPSS和Excel软件进行数据统计分析。结果显示:乌鲁木齐市犬布鲁氏菌病总流行率为25.5%(95%CI:20.9%~30.1%),且犬种和牛羊种并存,公共卫生威胁严重;从不同群体看,牧区流行率最高,为41.5%(95%CI:31.0%~52.0%);外购、散养、常接触牛羊、喂生肉、不处理犬粪便等行为是乌鲁木齐市犬布鲁氏菌感染的潜在风险因素,其中"常接触牛羊"(OR=1.97,95%CI:1.47~15.35)、"喂生肉"(OR=5.06,95%CI:1.69~20.74)是主要风险因素;乌鲁木齐市养犬人员对布鲁氏菌病的认知程度低,个人防护意识差。因此,应加强对犬感染牛羊种布鲁氏菌的公共卫生防控,减少犬与牛羊的直接接触,不喂生肉,定期开展犬布鲁氏菌病专项检测,同时加强宣传,提高养犬人员的布鲁氏菌病认知度。 相似文献
80.